scholarly journals The variation of essential oil and carvacrol contents of native grown Thymbra Spicata var. Spicata L.

2020 ◽  
Author(s):  
Kazim Mavi ◽  
Durmus Alpaslan Kaya ◽  
Musa Turkmen ◽  
Filiz Ayanoglu
Keyword(s):  
Essential Oil ◽  
Unit Area ◽  
Single Plant ◽  
Oil Glands ◽  
Code Number ◽  
Breeding Lines ◽  
Genetic Pool ◽  
Main Component ◽  
Selection Stage ◽  
Thymbra Spicata

In the study, it is aimed to create breeding lines of thyme (Thymbra spicata L.), which is important both culturally and economically, by selecting a single plant among the plants grown in different regions in Hatay. For this purpose, a genetic pool was created for Thymbra spicata L. plant in the plant samples taken from the locations where the plants are densely grown, and these plants were examined in terms of leaf characteristics, number of oil glands per unit area, oil gud size and essential oil components. Plants were propagated and preserved with cuttings taken from these single plants. In this study, which includes the pre-selection stage, 213 plants from 68 different locations were determined in the province of Hatay. The essential oil ratios of the plants varied between 0.70% and 3.90% and showed a wide variation. The rate of carvacrol, which is the main component of the essential oil of the thyme plant, was between 28.12% and 78.48%. Plants with code number Z14, Z3, Z25, Z38, Z77, Z104, Z35 and Z43 with an essential oil ratio of 3.5% and above and plants with code number Z167, Z165 and Z64 with a high carvacrol ratio were selected to be used in future breeding studies.

scholarly journals Oil glands number and oil glands diameters of Thymbra spicata var. Spicata L. leaves

2020 ◽  
Author(s):  
Durmus Alpaslan Kaya ◽  
Filiz Ayanoglu
Keyword(s):  
Essential Oil ◽  
Gene Pool ◽  
Unit Area ◽  
Average Diameter ◽  
Plant Leaves ◽  
Oil Glands ◽  
Plant Samples ◽  
Genetic Pool ◽  
Thymbra Spicata

In the study, a genetic pool was created with plant samples taken from the locations where the Thymbra spicata var. spicata L. plant, which is culturally and economically important, grows densely in the province of Hatay. Plants were propagated with cuttings taken from these single plants. 213 plants were collected from 68 different locations for the genetic pool. The leaves of the plants in this gene pool were examined in terms of the number of essential oil glands per unit area and the diameter of the essential oil glands. The number of glands per unit area in plant leaves showed a wide variation and ranged from 5.61 to 56.04 pieces/mm². The diameters of the oil glands varied between 75.40 - 112.86 µm and the average diameter was determined as 94.09 µm. In the study, it was determined that some plants with low essential oil ratios such as Z144 and Z158 also have low oil glands number and oil glands diameter. It would be appropriate to evaluate these values obtained as preliminary knowledge together with future ontogenetic variability studies.

scholarly journals Behavioral and Antennal Responses of Tribolium confusum to Varronia globosa Essential Oil and Its Main Constituents: Perspective for Their Use as Repellent

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4393
Author(s):  
Cesar Auguste Badji ◽  
Jean Dorland ◽  
Lynda Kheloul ◽  
Dimitri Bréard ◽  
Pascal Richomme ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Confusum ◽  
High Dose ◽  
Synthetic Chemicals ◽  
Stored Product Pest ◽  
Indigenous Plant ◽  
Main Components ◽  
Electroantennogram Recordings ◽  
The Individual ◽  
Main Component

Essential oils of aromatic plants represent an alternative to classical pest control with synthetic chemicals. They are especially promising for the alternative control of stored product pest insects. Here, we tested behavioral and electrophysiological responses of the stored product pest Tribolium confusum, to the essential oil of a Brazilian indigenous plant, Varronia globosa, collected in the Caatinga ecosystem. We analyzed the essential oil by GC-MS, tested the effects of the entire oil and its major components on the behavior of individual beetles in a four-way olfactometer, and investigated responses to these stimuli in electroantennogram recordings (EAG). We could identify 25 constituents in the essential oil of V. globosa, with anethole, caryophyllene and spathulenole as main components. The oil and its main component anethole had repellent effects already at low doses, whereas caryophyllene had only a repellent effect at a high dose. In addition, the essential oil abolished the attractive effect of the T. confusum aggregation pheromone. EAG recordings revealed dose-dependent responses to the individual components and increasing responses to the blend and even more to the entire oil. Our study reveals the potential of anethole and the essential oil of V. globosa in the management of stored product pests.

Diterpenoid and phenolic contents of Sideritis hololeuca Boiss & Heldr. Apud Bentham with antioxidant and anticholinesterase activity

2020 ◽  
Vol 75 (5-6) ◽  
pp. 161-169
Author(s):  
Sema Carikci ◽  
Ahmet C. Goren ◽  
Turgut Kilic
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Reducing Power ◽  
Acetone Extract ◽  
Inhibition Activity ◽  
Phenolic Contents ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Main Component ◽  
Butyrylcholinesterase Inhibition

AbstractThe objective of this study is to determine essential oil, diterpenoid and phenolic contents of Sideritis hololeuca along with their biological activities. Phytol was found to be the main component of the essential oil. Seven known kaurane diterpenoids, siderol (140 mg, 0.16%, w/w), 7-acetoxy sideroxol (15 mg, 0.02%, w/w), eubol (6 mg, 0.01%, w/w), eubotriol (5 mg, 0.03%, w/w), 7-epicandicandiol (3 mg, 0.02%, w/w), ent-7α-acetoxy-18-hydroxykaur-16-ene (5 mg, 0.01%, w/w) and linearol [by liquid chromatography-tandem mass spectrometry (LC-MS/MS)] were determined from the species. Moreover, vanillin (21 mg, 0.10%, w/w), which was not isolated from any Sideritis species earlier, was isolated from an acetone extract of S. hololeuca. Quantitative amounts of some phenolic compounds in n-hexane, dichloromethane, acetone, methanol extracts and infusion and decoction of the plants were also investigated by LC-MS/MS. Antioxidant capacity and acetylcholinesterase, butyrylcholinesterase inhibition effects of the species were evaluated. The extracts of methanol and infusion and decoction of species showed moderate butyrylcholinesterase inhibition activity. The highest inhibition was observed from the decoction of species. The Cu2+ reducing power of infusion was determined as 1.435 mmol TR g−1.

scholarly journals Antiprotozoal Activity of Turkish Origanum onites Essential Oil and Its Components

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4421 ◽  
Author(s):  
Tasdemir ◽  
Kaiser ◽  
Demirci ◽  
Demirci ◽  
Baser
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Mammalian Cells ◽  
Leishmania Donovani ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Flame Ionization ◽  
Main Component ◽  
Origanum Onites

Essential oil of Origanum species is well known for antimicrobial activity, but only a few have been evaluated in narrow spectrum antiprotozoal assays. Herein, we assessed the antiprotozoal potential of Turkish Origanum onites L. oil and its major constituents against a panel of parasitic protozoa. The essential oil was obtained by hydrodistillation from the dried herbal parts of O. onites and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). The in vitro activity of the oil and its major components were evaluated against Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani, and Plasmodium falciparum. The main component of the oil was identified as carvacrol (70.6%), followed by linalool (9.7%), p-cymene (7%), γ-terpinene (2.1%), and thymol (1.8%). The oil showed significant in vitro activity against T. b. rhodesiense (IC50 180 ng/mL), and moderate antileishmanial and antiplasmodial effects, without toxicity to mammalian cells. Carvacrol, thymol, and 10 additional abundant oil constituents were tested against the same panel; carvacrol and thymol retained the oil’s in vitro antiparasitic potency. In the T. b. brucei mouse model, thymol, but not carvacrol, extended the mean survival of animals. This study indicates the potential of the essential oil of O. onites and its constituents in the treatment of protozoal infections.

scholarly journals In VitroEffect ofCinnamomum zeylanicumBlume Essential Oil onCandidaspp. Involved in Oral Infections

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Marianne de Lucena Rangel ◽  
Sabrina Garcia de Aquino ◽  
Jefferson Muniz de Lima ◽  
Lúcio Roberto Castellano ◽  
Ricardo Dias de Castro
Keyword(s):  
Cell Wall ◽  
Essential Oil ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Fungal Growth ◽  
Human Cells ◽  
Phytochemical Analysis ◽  
Oral Infections ◽  
Human Red Blood Cells ◽  
Main Component

The present study demonstrates the antifungal potential of chemically characterized essential oil (EO) ofCinnamomum zeylanicumBlume onCandidaspp. biofilm and establishes its mode of action, effect on fungal growth kinetics, and cytotoxicity to human cells. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values varied from 62.5 to 1,000μg/mL, and the effect seems to be due to interference with cell wall biosynthesis. The kinetics assay showed that EO at MICx2 (500μg/mL) induced a significant (p < 0.05) reduction of the fungal growth after exposure for 8 h. At this concentration, the EO was also able to hinder biofilm formation and reduceCandidaspp. monospecies and multispecies in mature biofilm at 24 h and 48 h (p < 0.05). A protective effect on human red blood cells was detected with the EO at concentrations up to 750μg/mL, as well as an absence of a significant reduction (p > 0.05) in the viability of human red blood cells at concentrations up to 1,000μg/mL. Phytochemical analysis identified eugenol as the main component (68.96%) of the EO.C. zeylanicumBlume EO shows antifungal activity, action on the yeast cell wall, and a deleterious effect onCandidaspp. biofilms. This natural product did not show evidence of cytotoxicity toward human cells.

scholarly journals In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

2018 ◽  
Vol 7 (9) ◽  
pp. 283 ◽  
Author(s):  
Sherif Hassan ◽  
Kateřina Berchová-Bímová ◽  
Miroslava Šudomová ◽  
Milan Malaník ◽  
Karel Šmejkal ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Clinical Practice ◽  
Essential Oil ◽  
Biological Activities ◽  
Structural Parameters ◽  
Antihypertensive Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Ic50 Values ◽  
Main Component

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 µg/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 µg/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 µg/mL compared with that of cisplatin (IC50 = 10.08 µg/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 µg/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 µg/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 µg/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

scholarly journals Karakterisasi Minyak Atsiri dari Simplisia Basah Ranting dan Daun sebagai Alternatif Subtitusi Kulit Batang Cinnamomum burmannii Blume

2018 ◽  
pp. 125-136
Author(s):  
Mery Budiarti ◽  
Wahyu Jokopriambodo ◽  
Ani Isnawati
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Chemical Constituent ◽  
Raw Material ◽  
Alternative Source ◽  
Water Distillation ◽  
Marker Compound ◽  
Cinnamomum Burmannii ◽  
Main Component ◽  
Material Scarcity

The bark of Cinnamomum burmannii Blume is the main raw material of Cinnamomum oil because its cinnamaldehyde content is higher than in the other parts. This condition lead to the exploitation of Cinnamomum burmannii Blume bark without any cultivation effort, thus it can cause the raw material scarcity. Twigs and leaves of Cinnamomum burmannii Blume are also known to contain cinnamaldehyde therefore it provide a potential alternative source of cinnamaldehyde. The purpose of this research is to investigate the characteristic of Cinnamomum burmannii Blume essential oil which includes refractive index, profiling of chemical constituent and percentage of cinnamaldehyde as a marker compound. This research used three fresh Cinnamomum burmannii Blume simplicia of bark, twigs and leaves from two selected growing locations: Tawangmangu and Purwokerto. Simple water distillation method was conducted, together with characterization and refractive index using refractometer, followed by qualitative and quantitative analysis with Thin Layer Chromatography (TLC) and Gas Chromatography (GC). The results showed Cinnamomum burmannii Blume oil derived from bark, twig and leaf have different profiles, but all have cinnamaldehyde as the main component. The leaf produces higher essential oil yield percentage compared to the twig with a cinnamaldehyde content that is comparable to the bark by 50% of cinnamaldehyde content in cinnamomum bark oil.

Essential oil variation of Thymbra spicata L. (Lamiaceae), an East Mediterranean “oregano” herb

2018 ◽  
Vol 80 ◽  
pp. 63-69 ◽  
Author(s):  
Anastasia Stefanaki ◽  
Catherine Margaret Cook ◽  
Thomas Lanaras ◽  
Stella Kokkini
Keyword(s):  
Essential Oil ◽  
East Mediterranean ◽  
Thymbra Spicata

scholarly journals Study on the Improvement of Essential Oil Quality and Its Repellent Activity of Betel Leaves Oil (Piper betle l.) from Indonesia

2018 ◽  
Vol 34 (6) ◽  
pp. 2913-2926 ◽  
Author(s):  
Dante Alighiri ◽  
Edy Cahyono ◽  
Willy Tirza Eden ◽  
Ersanghono Kusuma ◽  
Kasmadi Imam Supardi
Keyword(s):  
Citric Acid ◽  
Essential Oil ◽  
Sodium Carbonate ◽  
Iron Content ◽  
Oil Quality ◽  
Acid Value ◽  
Piper Betle ◽  
Significant Difference ◽  
Main Component

The study to improve the quality of Indonesia betel leaves oil is done by treatment of citric acid and sodium carbonate. The treatment showed that the iron content and acid value were reduced optimally in the addition of 5% (w/w) citric acid (12 hours, 50oC) and 5% (w/w) sodium carbonate (9 hours, 50oC) yielded 0.45 mg/L of iron content and 1.00 mg/g of acid value, respectively. The main component of Indonesia betel leaves oil are 4-allyl phenylacetate. The descriptive sensory analysis showed that there were differences between betel leaves oil from Bogor compared with Boyolali and Makassar. There is a significant difference that the content of betel leaves oil from Bogor contains an isoeugenol compound of 20.71% while, Boyolali and Makassar contain eugenol of 17.02 and 19.21%, respectively. The study of the insect repellency indicated that Indonesia betel leaves oil potentially used as mosquito of Aedes aegypti repellent.

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