Thiol-disulphide homeostasis in essential thrombocythemia patients

Summary Background This study aimed to show the status of thioldisulphide homeostasis in essential thrombocytosis patients, which is known to play a role in platelet function. Methods The study included 27 ET patients and a control group of 36 healthy subjects. Serum total (–SH + –S–S–) and native (–SH) thiol levels were measured in all subjects using an automatic method. Results Age and gender distribution were similar in both groups. Compared with the control group, in the ET group, there were increased native thiol and total thiol levels (p = 0.001, p = 0.046). There was no correlation between thiol, total thiol and disulphide ratios with Jak2 mutation, hemorrhage and thrombosis. A positive correlation was determined between thrombosis and thiol disulphide homeostasis (p = 0.058). The study results showed that thiol-disulphide homeostasis shifted to the proliferative side in ET, in which ineffective erythropoiesis was predominant. It is also known that platelets are more active in ET cases and thiol disulphide balance is important in platelet function. Conclusions This result suggests that thrombotic complications may be reduced if the formation is achieved of mechanisms (oxidation mechanisms) that will trigger the increase of disulphide groups. However, more extensive research is needed on this subject.

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Assessment of dynamic thiol/disulfide homeostasis in patients with asthma

LaboratoriumsMedizin ◽

10.1515/labmed-2017-0144 ◽

2018 ◽

Vol 42 (3) ◽

pp. 99-104

Author(s):

Rukiye Nar ◽

Aliye Gamze Çalış

Keyword(s):

Oxidative Stress ◽

Forced Expiratory Volume ◽

Control Group ◽

Reactive Protein ◽

Total Thiol ◽

Stress Markers ◽

Group A ◽

And Gender ◽

And Control

Abstract Background: Asthma is a chronic inflammatory lung disease and oxidative stress is an important component in airway inflammation. This study aims to investigate dynamic thiol/disulfide homeostasis in patients with asthma. Methods: A total of 103 subjects, including 56 patients with asthma and 47 healthy controls, of similar age and gender were included in the study. The native thiol, total thiol and disulfide levels and the disulfide-native thiol, disulfide-total thiol and native thiol-total thiol ratios were analyzed and compared between the asthma and control groups using a novel automatized spectrophotometric assay. Results: The levels of native thiol (p<0.001), total thiol (p<0.001) and disulfide (p<0.001) were significantly lower and the C-reactive protein (CRP) levels (p<0.001) were significantly higher in patients with asthma when compared with those in the control group. A negative correlation was detected between CRP levels and native thiol, total thiol and disulfide levels (p<0.05). A significant positive correlation was detected between forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) levels and native thiol and total thiol levels (p<0.01). Conclusions: The thiol/disulfide homeostasis parameters may be used as novel oxidative stress markers in asthma but further studies are needed to investigate the role of thiol/disulfide homeostasis in asthma.

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RATIO OF MAIN PHYLOTYPES OF GUT MICROBIOTA IN PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE DEPENDING ON THE BODY MASS INDEX

Wiadomości Lekarskie ◽

10.36740/wlek202103126 ◽

2021 ◽

Vol 74 (3) ◽

pp. 523-528

Author(s):

Galyna D. Fadieienko ◽

Nataliia I. Chereliuk ◽

Valentina Yu. Galchinskaya

Keyword(s):

Body Mass Index ◽

Gut Microbiota ◽

Body Mass ◽

The Body ◽

Control Group ◽

Healthy Individuals ◽

Alcoholic Fatty Liver ◽

The Status ◽

And Gender ◽

Alcoholic Fatty Liver Disease

The aim: To analyze the status of Gut microbiota (GM) at the level of the main phylotypes in patients with NAFLD, depending on the body mass index (BMI) and gender in comparison with a group of practically healthy individuals. Materials and methods: The study involved 120 patients with NAFLD, who were divided into two groups depending on BMI and the control group containing 20 practically healthy individuals. Results: In patients with NAFLD with comorbid obesity, a statistically significant increase in the relative amount of Firmicutes (52.12 [42.38; 67.39]%) and Firmicutes/Bacteroidetes ratio (3.75 [1.7; 9.5]) against the background of a significant decrease in the amount of Bacteroidetes (13.41 [7.45; 26.07]%); in NAFLD patients with overweight, the relative amount of Firmicutes was 49.39 [37.47; 62.73]%, Firmicutes / Bacteroidetes ratio was 1.98 [1.15; 5.92], and the relative amount of Bacteroidetes was 23.69 [12.11; 36.16]%. In the control group, the distribution of the basic GM phylotypes was significantly different; the relative amount of Bacteroidetes was almost the same as of Firmicutes — 34.65 [24.58; 43.53]% and 29.97 [22.52; 41.75]% respectively, and the Firmicutes/Bacteroidetes ratio was 0.64 [0.52; 1.47]. Conclusions: The most statistically significant changes in the composition of IM occur due to the increase in the relative amount of Firmicutes and the ratio of Firmicutes/ Bacteroidetes against the background of a decrease in the relative amount of Bacteroidetes. These changes were directly proportional to the increase in BMI, but had no gender features.

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Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury

BioMed Research International ◽

10.1155/2018/1898671 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Ganime Ayar ◽

Sanliay Sahin ◽

Mutlu Uysal Yazici ◽

Salim Neselioglu ◽

Ozcan Erel ◽

...

Keyword(s):

Oxidative Stress ◽

Kidney Injury ◽

Pediatric Intensive Care ◽

Control Group ◽

Total Thiol ◽

Before And After ◽

Low Levels ◽

And Gender ◽

Positive Effect ◽

And Oxidative Stress

Aim. To evaluate thiol/disulphide homeostasis as a new indicator of oxidative stress in AKI patients and to determine the effect of HD on antioxidant balance and oxidative stress through plasma thiols. Methods. This study was performed in patients aged between 12 months and 18 years prospectively who underwent hemodialysis due to AKI and were followed up for a year in a 22-bed tertiary pediatric intensive care unit. 20 patients and 39 controls were included. Results. No difference was present between the groups in terms of age and gender. Median values of plasma native thiol, total thiol, and percent thiol were significantly lower in AKI group both before and after dialysis when compared to control group. The median dynamic disulphide values were significantly lower in the AKI group of predialysis compared to the controls. When pre- and postdialysis values were compared, disulphide values were statistically higher after dialysis. When pre- and postdialysis native thiol, dynamic disulphide, total thiol, and percent thiol median values were compared, postdialysis values were significantly higher than the predialysis values. There was a positive correlation between albumin, total thiol, and native thiol values before dialysis in the patient group. Conclusion. AKI patients have low levels of thiol species showing the presence of oxidative stress and hemodialysis has a positive effect on thiol/disulphide balance. This new method may be an inexpensive and simple tool suitable for clinical studies and can be used in routine screening as a useful indicator to show oxidative stress.

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Is There a Supporting Value of Platelet Distribution Width and Plateletcrit in the Diagnosis of Acute Appendicitis in Children?

Journal of Pediatric Infectious Diseases ◽

10.1055/s-0039-1685466 ◽

2019 ◽

Vol 14 (04) ◽

pp. 176-179

Author(s):

Salim Bilici ◽

Tülin Öztaş ◽

Ahmet Dursun

Keyword(s):

Acute Appendicitis ◽

Demographic Data ◽

Diagnostic Value ◽

Control Group ◽

Healthy Children ◽

Platelet Distribution Width ◽

Blood Cell Count ◽

Distribution Width ◽

Study Results ◽

And Gender

AbstractThis study was designed to investigate the diagnostic value of platelet distribution width (PDW) and plateletcrit (PCT) for acute appendicitis in childhood. A retrospective review was conducted of the records of 373 patients younger than 18 years who underwent appendectomy. Demographic data, white blood cell count, neutrophil count, and PDW and PCT values were recorded for all patients. A control group was formed of age- and gender-matched healthy children. The study results showed that the PDW level is not valuable as a marker for the diagnosis of acute appendicitis in children. PCT levels were determined to be statistically significantly increased in pediatric acute appendicitis with low specificity.

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Discourse on Leadership and Gender Awareness in Higher Education Publications: A View through the Lens of Feminist Phase Theory

Journal for the Study of Postsecondary and Tertiary Education ◽

10.28945/3431 ◽

2016 ◽

Vol 1 ◽

pp. 103-120

Author(s):

Adrienne E. Hyle ◽

Peggy M. Delmas ◽

Bernita L. Krumm

Keyword(s):

Higher Education ◽

Leadership Development ◽

Analytical Framework ◽

Phase Theory ◽

Higher Education Journals ◽

Study Results ◽

The Status ◽

Gender Awareness ◽

And Gender ◽

Feminist Phase Theory

Using Feminist Phase Theory (FPT) as our analytical framework, we studied the status of gender awareness and influence in higher education leadership development trends in four premier higher education journals for the years 2008, 2011, and 2014. Our analysis was accomplished through the review of articles and book reviews published in two US and two international journals: Higher Education (Netherlands), Higher Education Quarterly (UK), Journal of Higher Education (US), and The Review of Higher Education (US). Study results indicated progress toward a multifocal set of perspectives in which gender was not an issue; rather other concerns such as social justice or diversity were the focus. Data also indicated that while gender was no longer a specific focus of the literature, it was still an underlying concern. Gender and leadership are still being examined, intentionally or not. An additional finding revealed through the study of these journals is a lack of research about leadership in higher education, particularly in the US. A focus on understanding leadership does not appear to be a priority among this higher education community.

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Effektivnost' primeneniya preparata«Magnerot®» pri proyavleniyakh sindromasosudistykh narusheniy i gemorragicheskogosindroma u bol'nykh s idiopaticheskimprolapsom mitral'nogo klapana

Cardiosomatics ◽

10.26442/cs44956 ◽

2011 ◽

Vol 2 (1) ◽

pp. 13-17

Author(s):

Evgeniya Vladimirovna Akatova ◽

Olesya Petrovna Nikolin ◽

Anatoliy Ivanovich Martynov

Keyword(s):

Severe Form ◽

Controlled Study ◽

Control Group ◽

Study Group ◽

Vascular Disorders ◽

Study Results ◽

Comprehensive Examination ◽

The Mean ◽

And Gender ◽

Single Blind

Summary. Aim. To evaluate the efficiency of placebo-controlled magnerot use on vascular and hemorrhagic manifestations in patients with idiopathic mitral valve prolapse (MVP). Subjects and methods. Seventy-four patients (31,7% males and 68,3% women) with idiopathic MVP were examined during a single-blind placebo-controlled study. They were randomized into 2 groups: 1) magnerot (a study group) and 2) placebo (a control group). The mean age of the study group patients was 30,80,4 years. The placebo group was matched by age (mean age 31,10,2) and gender. There was a female preponderance among the examinees in both groups. The study group patients received MagnerotR tablets containing 500 mg of Mg orotate (32,8 Mg of elementary Mg) in a daily dose of 3000 mg (196,8 mg of elementary Mg) for 6 months. All the patients underwent comprehensive examination before and 6 months after the study. Results. A clinical significant reduction in the degree of vascular disorders was noted in 71,3% of the patients with MVP. The incidence of vascular disorders remained virtually unchanged in the study group. Vascular disorders were significantly alleviated after the therapy. If before the therapy, mild, moderate, and severe vascular disorders were diagnosed in 30,2, 55,9, and 13,9% of cases, respectively; whereas after the therapy these were absent in 16,3% of cases; the patients with mild vascular disorders increased by 2,5-fold and their severe form was undetected. The clinical effect of the therapy on the severity of hemorrhagic syndrome was observed in 81,4% of patients (75% males and 89,5% females) and the therapeutic efficiency that very frequently corresponded to clinical improvement was seen in 2,3%. Conclusion. The effect of magnerot therapy is shown to significantly reduce the incidence and degree of vascular disorders and hemorrhagic syndrome and their clinical picture as a whole.

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POS1377 SOME PARAMETERS OF ENDOTHELIAL FUNCTION IN PATIENTS WITH IDIOPATHIC AVASCLAR NECROSIS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.3699 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 970.2-971

Author(s):

K. Gudkevich ◽

N. Martusevitch ◽

E. Dashkevich

Keyword(s):

Femoral Head ◽

Endothelial Function ◽

Vitamin B12 ◽

Microvascular Dysfunction ◽

Control Group ◽

Secondary Osteoarthritis ◽

Treatment And Prevention ◽

Study Results ◽

And Gender ◽

Femoral Condyles

Background:Avascular necrosis (AN) is a disease, which is the result of blood flow disturbance and necrosis of the bone tissue. It affects predominantly men of working age and steadily progresses to secondary osteoarthritis. AN is a multifactorial disease. In the context of vascular hypothesis it is of particular interest to evaluate homocysteine and its metabolism in patients with AN.Objectives:The aim of the present study was to evaluate some parameters of endothelial function in patients with AN of the femoral head or femoral condyles in order to determine possible treatment and prevention options.Methods:We included 53 patients with AN of the femoral head or femoral condyles into the study. The patients were aged 49 (35-62) years, 58,49% (n=31) were male. The inclusion criteria was diagnosis of AN confirmed by X-Ray, CT or MRI. The exclusion criteria were: traumatic AN, Legg-Calve-Pertes disease, connective tissue systemic diseases. We recruited 51 patients into the control group. The groups were comparable by age and gender. In order to analyse engothelial function in the groups we investigated the following serum parameters: homocysteine, vitamin B12, folate. In order to analyse the data obtained we used non-parametric statistics.Results:We determined a higher level of homocysteine in patients with AN (12,15 (7,55-14,60) vs. 8,18 (6,57-10,70) mcmol/l in the control group, p=0,04). Also we observed almost equal levels of vitamin B12 in patients with AN an in the control group (531,95 (340,80-793,55) vs. 532,75 (417,60-669,7) pg/ml, p=0,99). The level of folate was lower in the study group (5,66 (3,83-6,83) vs. 6,99 (5,02-10,38) ng/ml, p=0,01).Conclusion:The data obtained show that homocysteine level in patients with AN is higher. This can be a consequence of folate deficiency, which takes part in homocysteine metabolism. Therefore, homocysteine metabolism disturbances can lead to endothelial dysfunction, which results in cardiovascular events in the heart, brain, kidneys [1] as well as in the bone, according to the study results. Folate supplement is the possible way of AN treatment and prevention.References:[1]Nowroozpoor A, Gutterman D, Safdar B. Is microvascular dysfunction a systemic disorder with common biomarkers found in the heart, brain, and kidneys? - A scoping review. Microvasc Res. 2021 Mar;134:104123. doi: 10.1016/j.mvr.2020.104123. Epub 2020 Dec 15. PMID: 33333140.Disclosure of Interests:None declared

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Penatalaksanaan IMD pada Ibu Postpartum Sectio Caesarea Mempengaruhi Status Gizi dan Kecepatan Produksi ASI

Jurnal Bidan Cerdas (JBC) ◽

10.33860/jbc.v2i2.68 ◽

2020 ◽

Vol 2 (2) ◽

pp. 112-120

Author(s):

Nursari Abdul Syukur ◽

Susi Purwanti

Keyword(s):

Breast Milk ◽

Nutritional Status ◽

Milk Production ◽

Quantitative Research ◽

Milk Protein ◽

Sectio Caesarea ◽

Control Group ◽

P Value ◽

Study Results

Many mothers who give birth to Sectio Caesarea (SC) do not Initiate Early Breastfeeding (IMD), which fails exclusive breastfeeding. This study aimed to determine the effect of IMD management in postpartum SC mothers on nutritional status, speed of milk production, and quality of breast milk protein. Method: quantitative research with quasi approach experiment. The research design used was a pre-post-test control non-equivalent control group. A sampling of this study used the Consecutive method sampling with a sample of 20 mothers who gave birth by cesarean section (SC). Hypothesis testing uses the independent t-test and the Mann-Whitney test. The study results showed an influence on the management of IMD in postpartum SC mothers on the speed of ASI production (p-value=0.004) and nutritional status (p-value=0.028). There was no effect of IMD management on postpartum SC mothers on the quality of breast milk protein (p-value = 0.543). This study recommends that the hospital implement an IMD promotion program before the abdominal wall is closed as a form of intervention to increase milk production and maternal nutritional status

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Moderating Role of Gender in the Relationship between Cognitive Errors and Anxiety among Adolescents

Foundation University Journal of Psychology ◽

10.33897/fujp.v4i1.68 ◽

2020 ◽

Vol 4 (1) ◽

pp. 86-102

Author(s):

Tasnim Rehna ◽

Rubina Hanif ◽

Muhammad Aqeel

Keyword(s):

Mental Health Problems ◽

Adolescent Psychopathology ◽

Future Research ◽

Cognitive Errors ◽

Adolescent Anxiety ◽

Study Results ◽

The Status ◽

Manifest Anxiety ◽

The Relationship

Background: Widespread social paradigms on which the status variances are grounded in any society, gender plays pivotal role in manifestation of mental health problems (Rutter, 2007). A hefty volume of research has addressed the issue in adults nonetheless, little is vividly known about the role of gender in adolescent psychopathology. Sample: A sample of 240 adolescents (125 boys, 115 girls) aging 12-18 years was amassed from various secondary schools of Islamabad with the approval of the Federal Directorate of Education (FDE), relevant authorities of the schools and the adolescents themselves. Instruments: Taylor Manifest Anxiety Scale (Taylor & Spence, 1953) and Children’s Negative Cognitive Errors Questionnaire (CNCEQ) by Leitenberg et al., (1986) were applied in present study. Results: Multiple regression analysis revealed that cognitive errors jointly accounted for 78% of variance in predicting anxiety among adolescents. Findings also exhibited that gender significantly moderated the relationship between cognitive errors and adolescent anxiety. Implications of the findings are discoursed for future research and clinical practice.

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Thromboembolic complications prevention in the obstetric practice

HEALTH OF WOMAN ◽

10.15574/hw.2017.117.19 ◽

2017 ◽

pp. 19-24

Author(s):

O.V. Grishchenko ◽

◽

V.V. Bobrytska ◽

Keyword(s):

Pulmonary Embolism ◽

Comparison Group ◽

Main Group ◽

Control Group ◽

Efficacy And Safety ◽

Thromboembolic Complications ◽

Moderate Risk ◽

Obstetric Practice ◽

Thrombotic Complications ◽

And Control

The objective: To evaluate the clinical efficacy and safety of Enoxaparin-Pharmex for the prevention of thrombotic complications (pulmonary embolism) in the postoperative period in patients with moderate risk of these complications. Patients and methods. The study included 50 women after a caesarean section had an average degree of risk of pulmonary embolism. Patients were divided into the main group (n=25) and control group (n=25) in accordance with the treatment: patients of the main group received postoperative Еnoxaparin- Pharmex, group comparisons enoxaparin sodium (brand foreign manufacturer’s). Patients in both groups received the drug at a dose of 20 mg for 5 days, 1 time per day subcutaneously. Results. The research data analysis showed identity results of hemostasiogram of patients in the main group and the comparison group, no side effects after treatment in both groups. Conclusion. The clinical studies suggest the drug Enoxaparin-Pharmex is effective, safe LMWH, which can be used to prevent troboembolic complications, including post-operative treatment in obstetric practice. Spectrum of Enoxaparin-Pharmex can be extended to the prevention and treatment of thromboembolic conditions of varying severity with appropriate doses of the drug. Key words: Enoxaparin-Pharmex, prevention of pulmonary embolism.

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