A pilot study on the effect of a novel feed additive containing exogenous enzymes, acidifiers, sodium butyrate and silicon dioxide nanoparticles on selected cellular immune indices and body weight gains of calves

Abstract Introduction The rearing of calves is a difficult period for farmers due to health problems to which the animals are prone this time. Since the use of antibiotics as growth promoters has been forbidden, various innovative feed additives have been tested in many countries around the world. Material and Methods In this study, experimental (E) calves were supplemented with a novel feed additive consisting of the pancreatic-like enzymes protease and lipase, a fat-coated mixture of organic fumaric, malic, citric and sorbic acids, sodium butyrate and silicon dioxide nanoparticles. Control (C) calves received feed without additive. During the supplementation, white blood cell (WBC) counts with leukocyte differentiation, percentages of B lymphocytes and T lymphocytes and their subpopulations, phagocytic activity and oxidative burst of circulating monocytes and granulocytes were examined. Body weight (b.w.) gains of the calves were also monitored. Results The WBC counts in the E and C calves were within the reference ranges throughout the study. In the analysis of the percentages of the lymphocyte subpopulations, phagocytic activity and oxidative burst, no statistically significant differences were reported between the E and C groups. However, higher average daily body weight gains were obtained for the E calves. Conclusion The study revealed that the examined feed additive did not modulate the immune response of the calves significantly. The tendency to higher daily average b.w. gains in the E calves than in the C calves suggests a beneficial effect of this feed additive.

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Efficacy of feed additives in enhancing performance of growing pigs

Animal Science ◽

10.1017/s0003356100023849 ◽

1980 ◽

Vol 30 (1) ◽

pp. 95-103 ◽

Cited By ~ 4

Author(s):

T. L. Veum ◽

R. Lauxen ◽

J.-T. Yen

Keyword(s):

Body Weight ◽

Weight Gain ◽

Feed Intake ◽

Live Weight ◽

Growing Pigs ◽

Feed Additives ◽

Feed Additive ◽

Control Treatment ◽

Feed Ratio ◽

Weight Gains

ABSTRACTThe efficacy of feed additives in enhancing the performance of growing pigs, together with the effect of different protein levels (18 v. 16% from 13 to 18 kg body weight and 16 v. 14% from 18 to 39 kg body-weight), were evaluated in three experiments with 412 pigs. In Experiment 1, from 13 to 39 kg body weight, pigs receiving 55 p.p.m. carbadox (C) produced a greater (P<0·05) average daily live-weight gain than did the non-medicated (NM) pigs and those receiving 275 p.p.m. chlortetracycline-sulphamethazine-penicillin (ASP) with a similar but non-significant (P>0·05) trend n i daily feed intake and gain/feed ratio. The ASP pigs also produced a greater (P<0·05) weight gain than the NM pigs. The higher dietary protein level sequence produced a greater (JP<0·05) weight gain and gain/feed ratio.In Experiment 2, from 16 to 39 kg body weight, NM control pigs achieved high weight gains, and all the feed additive treatments studied [C+106 p.p.m. of the worming agent pyrantel tartrate (C+P); ASP+8 mg of the worming agent levamisole-HCl per kg body weight fed once on day 7 of the experiment (ASP+L); and 113 p.p.m. tylosin+13 p.p.m. of the worming agent hygromycin B] were similar to the NM controls in daily feed intake, weight gain and gain/feed ratio. However, in Experiment 3, the C+P and ASP+L treatments resulted in a greater (P<0·05) weight gain with a trend toward greater daily feed intake than did the NM control treatment from 17 to 35 kg. The weight gain of the ASP+L pigs was also greater (P<0·05) than that of 165 p.p.m. oxytetracycline and NM pigs from 35 to 57 kg. From 57 to 96 kg all the pigs received the same NM control diet and their weight gains were similar. The gain/feed ratio was similar among the treatments within each of the weight periods in Experiment 3. Pigs slaughtered at the beginning and at various stages during Experiments 2 and 3 were free of internal parasites and migration damage.

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System of antioxidant protection of the body of piglets under the action of feed additive “Sylimevit”

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet10402 ◽

2021 ◽

Vol 23 (104) ◽

pp. 10-17

Author(s):

L. V. Vyslotska ◽

B. V. Gutyj ◽

O. V. Kozenko ◽

V. I. Khalak ◽

M. V. Chornyj ◽

...

Keyword(s):

Body Weight ◽

Antioxidant Defense System ◽

Feed Additives ◽

The Body ◽

Biologically Active ◽

Feed Additive ◽

Free Access ◽

Direct Acting ◽

Active Substances ◽

Technological Stress

One of the conditions for obtaining high-quality pig products is the use of feed additives, which contain all the necessary biologically active substances, eliminating their deficiency in feed and acting as catalysts for metabolic processes in pigs. That is why the aim of the study was to study the effect of feed additive “Sylimevit” on the enzymatic and non-enzymatic parts of the antioxidant system of piglets after weaning. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogues – age, breed and body weight. During the weaning period, the piglets were kept under the sow in special machines, had constant access to the mother, and from the age of 5 days – free access to concentrated feed. Feeding of animals was carried out in accordance with the norms for a given age of pigs. Prior to the study, a clinical and physiological examination of the piglets was performed. Their general condition and activity when eating food were taken into account. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests in order to be further maintained during the period of fattening and rearing with a change in the structure of the diet, which served as technological stress for the animals. From the age of 5 days, piglets of all groups were fed pre-starter feed. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed supplement “Sylimevit” at a dose of 100 mg/kg body weight per day. Feeding piglets Silimevit feed enhances their antioxidant status. The use of this feed additive in piglets contributed to the activation of both enzymatic and non-enzymatic parts of the antioxidant defense system of piglets after weaning, as indicated by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.9 % (P < 0.001), catalase – 41.1 % (P < 0.001), glutathione peroxidase – by 52.7 % (P < 0.05), glutathione reductase – by 53.5 % (P < 0.05), the content of reduced glutathione – by 75 % (P < 0.001). This is due to the fact that silimevit contains active substances such as milk thistle and vitamins, which are strong direct-acting antioxidants and directly interact with free radicals and reactive oxygen species.

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«Energosil» in the diets of replacement gilts

Agricultural science Euro-North-East ◽

10.30766/2072-9081.2019.20.2.153-162 ◽

2019 ◽

Vol 20 (2) ◽

pp. 153-162

Author(s):

A. A. Suvorov ◽

в Sh. Gayirbegov ◽

A. S. Fedin ◽

A. M. Guryanov

Keyword(s):

Body Weight ◽

Live Weight ◽

Feed Additives ◽

The Body ◽

Feed Additive ◽

Control Group ◽

Practical Training ◽

Optimal Dosage ◽

Growth Energy ◽

Experimental Group

The article gives scientific substantiation of using silicon-containing new feed additive “Energosil” for replacement gilts. The studies were conducted in pig-breeding complex of the Center for Practical Training of Agricultural Specialists in Mordovia Republic. There were three experimental groups of replacement gilts (10 animals each). The effect of various dosages of Energosil (5.0, 7.5, and 10 mg per kg of body weight) on digestibility, nutrient utilization, and animal growth energy was studied. The most optimal dosage of a new feed additive in their rations was revealed, which constituted 5 mg / kg of the animal live weight. It was established that the animals from the first experimental group, who received silicon-containing feed additives of this amount digested the nutrients of the diet better than their counterparts from the other groups. Compared to the control group, the digestibility of dry matter increased by 3.62% (p<0.05), organic matter by 3.29% (p<0.01), crude protein by 4.09% (p<0.001), fat by 4.30% (p<0.01), cellulose by 3.79% (p<0.01) and nitrogen-free extractives by 2.03% (p>0.05).Replacement gilts of the first experimental group, who were given Energosil in the amount of 5 mg / kg body weight in addition to the basic ration, retained nitrogen in their bodies by 17.1%, calcium by 55.9% and phosphorus by 69.7% more than their counterparts from the control group. It contributed to an increase in body weight of gilts from the first group compared to control counterparts by 10.8%. Increased dosages of Energosil (7.5 and 10 mg / kg body weight) also had a better effect on the growth energy of gilts compared with the control group. The feed additive in the amount of 7.5 mg kg of animal live weight contributed to an increase in animal weight compared with control counterparts by 6.21 kg or 4.8% (p<0.05), with a third experimental group by 4.4 kg or by 3.4% (p<0.05). When given Energosil in the dosage of 10 mg/kg, the body weight of gilts from the third experimental group was higher by 1.81 kg or 1.4% (p> 0.05) compared with the control counterparts.

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Influence of phytogenic feed additives and prebiotic in vegetable protein based diet on broiler performance

Progressive Agriculture ◽

10.3329/pa.v28i4.36372 ◽

2018 ◽

Vol 28 (4) ◽

pp. 323-330 ◽

Cited By ~ 1

Author(s):

HR Tanzim ◽

GB Das ◽

M Ahmad ◽

M Barua ◽

K Islam

Keyword(s):

Body Weight ◽

Weight Gain ◽

Feed Intake ◽

Vegetable Protein ◽

Experimental Period ◽

Feed Additives ◽

Feed Additive ◽

Control Group ◽

Feed Conversion ◽

Broiler Performance

This study was conducted to determine the effect of phytogenic feed additive and prebiotic on vegetable protein base diet in broiler performance. A total of 90 chicks (Cobb-500) were weighted and randomly assigned to the three treatment groups (To, T1 and T2) and supplemented with either phytogenic feed additives or prebiotic and reared for 28 days. Data on live body weight, body weight gain, feed intake and feed conversion (FC) were taken at weekly interval. Results revealed that significant (P<0.05) increase in live body weight of broilers was evident in both phytogenic feed additive and prebiotic supplemented groups comparing to the control group at 4th weeks of age. No significant (P>0.05) differences were found in live weight gain among the treatment groups on weekly intervals along the whole experimental period. However, significant differences (P<0.05) were evident on cumulative weight gain at 3rd and 4th weeks of age. In case of feed intake at the age of 3rd and 4th weeks, significant (P<0.05) differences were obtained with decreased feed intake in both phytogenic feed additive and prebiotic supplemented groups comparing to the control group. In case of feed conversion (FC), there were significant (P<0.05) differences among the groups on 1st week and 4th week of age. Best FC was accounted for prebiotic supplemented group along the whole experimental period. The survivability rates were 96.67%, 100% and 100% in control group, phytogenic feed additive and prebiotic supplemented group respectively. Results finally showed that vegetable protein when supplemented with phytogenic prebiotic feed additives and prebiotic c an additive beneficial effect on performance of when compared to non-supplemented control diet. So, ration using vegetable protein mixed with phytogenic feed additives or prebiotic can be recommended for broiler.Progressive Agriculture 28 (4): 323-330, 2017

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The effect of phytogenic feed additives on the performance, meat quality and coccidial infection rates of rabbits

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.8482 ◽

2017 ◽

Author(s):

Stanislaw Lapiñski ◽

Leszek Gacek ◽

Joanna Gawroñska ◽

Iwona Guja ◽

Jerzy Kowal ◽

...

Keyword(s):

Body Weight ◽

Meat Quality ◽

Food Additives ◽

Feed Additives ◽

Garlic Extract ◽

Control Group ◽

Weight Gains ◽

Coccidial Infection ◽

Positive Effect ◽

Herbal Supplementation

The aim of the study was to evaluate the effect of herbal supplementation on rabbits’ health and production parameters. In experiment with herbal food additives, at 90 days of age animals fed with phytogenic additives (oregano, garlic and rosemary) had higher body weight compared to the group fed with coccidiostat. In experiment with herbal water additives (garlic extract or oregano) the phytobiotic supplementation had no effect on weight gains of animals. The highest body weight on slaughter day was obtained by rabbits drank water with garlic extract. Parasitological tests performed at 91 days of age showed that lowest average number of coccidia oocysts per gram of faeces (OPG) in “garlic” group (48.0 OPG) and the highest in control group (308.6 OPG). The research showed that feed with herbal supplementation is willingly eaten by rabbits, contributes to faster weight gains and has a positive effect on meat quality.

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Evaluation of Roselle Hibiscus sabdariffa Flower as a Nutritive Additives on the Productive Performance of Broiler

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v37i1.335 ◽

2013 ◽

Vol 37 (1) ◽

pp. 69-74

Author(s):

Mamdooh A.M. Nasrawi

Keyword(s):

Body Weight ◽

Body Weight Gain ◽

Feed Additives ◽

Feed Consumption ◽

Feed Additive ◽

Control Group ◽

Broiler Chicks ◽

Productive Performance ◽

Feed Conversion ◽

Broiler Diet

The study included 300 unsexed broiler chicks (Hubbard) were used to evaluate (Roselle Flower) as a feed additives on the productive performance of broiler. The chicks were divided into five groups with three replicates (20 birds in each) and fed on five diets. Diet 1 (control group) dried Roselle flower powder were supplemented to the diet 2, 3, 4 and 5 as 0.25, 0.5, 0.75 and 1% respectively. The result revealed that a significant (P<0.05) increase in body weight, body weight gain, feed consumption and improvement in feed conversion ratio. Although the differences between groups for edible parts percent were not significant (Heart, Liver and Gizzard). The hematological values PCV%, RBC, WBC and H/L ratio were increased significantly (P<0.05) and cholesterol values decreased significantly as compared with control group. It can conclude that Roselle as other herbal plants may be considered as a feed additive in broiler diet would improve the productive performance, hematological and biochemical values of broiler. Diet Roselle flower supplementation of (1g/kg) might be acceptable for achieving optimum broilers performance.

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Growth and immune response of Litopenaeus vannamei fed on β-(1, 3) glucan and poly-β hydroxybutyrate

Jurnal Akuakultur Indonesia ◽

10.19027/jai.12.121-127 ◽

2015 ◽

Vol 12 (2) ◽

pp. 121

Author(s):

, Sarmin ◽

Muhammad Agus Suprayudi ◽

Dedi Jusadi ◽

Julie Ekasari

Keyword(s):

Immune Response ◽

Body Weight ◽

Growth Performance ◽

Litopenaeus Vannamei ◽

Pacific White Shrimp ◽

Feed Additives ◽

Specific Immune Response ◽

White Shrimp ◽

Feed Additive ◽

Initial Body Weight

ABSTRACT This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei) fed on the diet supplemented with β-(1,3) glucan (BG) and poly-β-hydroxybutyrate (PHB) as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives), 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response. Keywords: growth, shrimp, non-specific immune response, Litopenaeus vannamei ABSTRAK Penelitian ini bertujuan untuk menguji kinerja pertumbuhan dan respons imun nonspesifik udang vaname Litopenaeus vannamei yang diberi pakan dengan penambahan feed additive berupa β-(1,3) glukan (BG) dan poli-β-hidroksibutirat (PHB). Juvenil udang 2,06±0,03 g dipelihara pada 12 unit akuarium dengan empat perlakuan dan tiga ulangan, serta padat tebar 20 ekor/tank selama 42 hari pemeliharaan. Perlakuan yang diberikan dalam penelitian ini yaitu penambahan BG (1,5 g/kg), PHB (10 g/kg), dan BG (1,5 g/kg)+PHB (10 g/kg), serta kontrol (tanpa penambahan feed additive). Hasil penelitian menunjukkan bahwa udang yang diberi 1,5 g/kg BG memiliki kinerja pertumbuhan dan respons imun nonspesifik yang terbaik. Kata kunci: pertumbuhan, udang, respons imun nonspesifik, Litopenaeus vannamei

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Hepatic protein synthesis and morphological parameters in blood of rats under oxidative stress and action of feed additive Butaselmevit-plus

Ukrainian Journal of Ecology ◽

10.15421/2019_800 ◽

2019 ◽

Vol 9 (4) ◽

pp. 628-633

Author(s):

M. Ivankiv ◽

N. Kachmar ◽

O. Mazurak ◽

T. Martyshuk

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Protein Synthesis ◽

Feed Additives ◽

Male Rats ◽

Farm Animals ◽

Feed Additive ◽

Intragastric Administration ◽

Morphological Parameters ◽

Experimental Group

The intensive development of animal husbandry at the present stage requires new approaches to organizing the feeding of farm animals and the introduction of modern feed additives, which are not normally used purely as feed, but are deliberately added to feed or water to improve their quality, increase productivity and animal welfare. To prevent the development of oxidative stress in animals, we used the feed additive "Butaselmevit-plus", which in its composition contains the fruits of milk thistle, selenium, methionine and vitamins A, E and D3. The aim of the study was to investigate the effect of feed additive Butaselmevit-plus on blood morphological parameters and protein synthesis of rat liver under tetrachloromethane poisoning. For research, we used Wistar male rats, which were divided into three groups of 20 animals each. Control rats were intact. The first and second experimental group of rats were administered tetrachloromethane. Experimental intoxication in animals was performed by twice (after 48 h) intragastric administration of tetrachloromethane at a dose of 0.1 ml per 100 g of rat body weight in the form of a 50% oil solution. Experimental group R2 was fed a feed additive of Butaselmevit-plus for a dose of 0.1 g per 100 g of body weight together with the feed for experimental toxicosis for 30 days. On the basis of the conducted researches positive effect of feed additive "Butaselmevit-plus" on the organism of rats, which were intoxicated with tetrachloromethane, is shown, which is shown by normalization of hematological parameters and protein synthesis of liver function. Restoration of hematopoietic function of bone marrow of rats by intoxication with tetrachloromethane is due to the fact that the feed additive "Butaselmevit-plus" contains in its composition fruits thistle, which have a high level of vitamins A and K, and trace elements, namely: Ferum, Cuprum and Cobalt that are directly involved in hematopoiesis. When feeding Butaselmevit-plus as feed supplement, the number of red blood cells in the blood of the second experimental group of rats was higher than that of the first experimental group, but the control values reached only on the 30th day of the experiment. When using the feed additive Butaselmevit-plus in rats under conditions of oxidative stress development, the leukocyte count decreased to the optimum level.

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Probiotics and Herbs Combination in Commercial Feed Additives as Growth Promoter in Broiler Chicken

BIO Web of Conferences ◽

10.1051/bioconf/20213304008 ◽

2021 ◽

Vol 33 ◽

pp. 04008

Author(s):

Thomas Emanuel Manggotu Nahak ◽

Agnesia Endang Tri Hastuti Wahyuni ◽

Yanuartono ◽

Charles Rangga Tabbu

Keyword(s):

Body Weight ◽

Organic Acids ◽

Feed Additives ◽

Feed Additive ◽

Growth Promoter ◽

Broiler Performance ◽

Group Iii ◽

Group I ◽

Commercial Feed ◽

Significant Difference

The prohibition on the use of Antibiotic Growth Promoter (AGP) causes the use of probiotics, herbs, enzymes and organic acids as an alternatives to AGP. This study aimed to determine the role of combination of probiotic and herbs in commercial feed additives on broiler performance. Sixty day-old male broiler were divided into 3 groups where each group consisted of 20 chickens with 4 replication each group. Group I was given commercial feed, group II was given commercial feed + AGP and group III was given commercial feed + combination of probiotics, herbs, enzymes and organic acids. All chickens were kept for 5 weeks. Body weight gain (BWG), body weight (BW) and Feed Convertion Ratio (FCR) were calculated every week. Carcass percentage was calculated at week 5. The results obtained from BWG and BW on group III at weeks 4 and 5 weeks were higher and significant difference (P<0.05) compared to groups I and II. The percentage of carcasses between groups did not have a significant difference (P>0.05). FCR values in group III from week 1 to weeks 5 were lower than groups I and II. The use of combination of probiotics, herbs, enzymes and organic acids has proven to have a good role on broiler performance so that it can replace AGP as a feed additive on broiler.

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Evaluation of the use of feed additives of humic nature by indices of leukocytes in rats after combined stress

Bulletin of Taras Shevchenko National University of Kyiv Series Biology ◽

10.17721/1728_2748.2019.77.16-21 ◽

2019 ◽

Vol 77 (1) ◽

pp. 16-21

Author(s):

L. Diachenko ◽

L. Stepchenko

Keyword(s):

Body Weight ◽

Vitamin E ◽

Feed Additives ◽

The Body ◽

Stress Factors ◽

Feed Additive ◽

Control Group ◽

Combined Stress ◽

Active Forms Of Oxygen ◽

Functional Changes

In response to the action of stress factors of different genesis in a living organism, a cascade of adaptive responses, which can neutralize functional disorders of homeostasis, is triggered. Combining the effects of various stress factors can cause excessive formation and accumulation of active forms of oxygen, which entails a number of pathological conditions that create the preconditions for the development of a large number of diseases. One of the indicators of the development of adaptation and the integral indicator of the body's state is the blood system, especially the leukocytic formula, whose redistribution makes it possible to analyze the nature of the effect and estimate the intensity of adaptive processes in the body due to functions that perform different forms of leukocytes. Leukocyte indices in which the parameters of the leukocyte formula were used, allow assessment of the degree of endogenous intoxication, the development of resistance and the character of cellular immunity for the influence of stress factors on the organism of animals and humans.The article considers the possibility of evaluating the preventive effect of natural antioxidants of humic nature on indices of leukocytes on the organism of rats after the influence of combined water-immobilization stress (WICS). for this, the animals were divided into five groups of 6 animals: 1- group of intact animals (control); 2-5 – experimental groups. Animals of all experimental groups were additionally received orally, individually with the help of a probe for 18 days, water, feed additives "Humilid" (at 5 mg / kg of body weight per active ingredient), "Eco-Impulse Animal" (at a rate of 2.5 mg / kg body weight) and vitamin E (at a rate of 50 mg / kg body weight). In animals, 2, 3, 4 and 5 groups simulated stress. It has been established that the effects of water-immobilization combined stress on blood of rats lead to the growth of cells of the leukocyte series, mainly due to the increase of eosinophils and rod-neutron neutrophils. The use of natural antioxidant, a feed additive "Humilid" in the pre-season period causes a decrease in the number of leukocytes by 41% compared to values in animals that received purified water during this period and did not differ from those in intact animals. The change in leukocyte indexes indicates functional changes in the body and the presence of a neutrophilic shear on the background of WICS in rats. Also, the effect of WICS leads to the release of the younger forms of neutrophils in the bloodstream, an increase in both the percentage of eosinophils and the quantitative. For preventive use of feed additives of humic nature, "Humilid" and "Eco-Impulse Animal" and vitamin E, the studied parameters are close to the values of the control group.

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