MOLECULAR GENETIC MODIFICATIONS OF κ-CASEIN

2020 ◽  
Author(s):  
А.Г. КРУЧИНИН ◽  
С.Н. ТУРОВСКАЯ ◽  
Е.Е. ИЛЛАРИОНОВА ◽  
А.В. БИГАЕВА
Keyword(s):  
Molecular Genetic ◽  
Technological Properties ◽  
Formation Model ◽  
Casein Micelles ◽  
Genetic Modifications ◽  
Polymorphic Variants ◽  
Resistance Decrease ◽  
Casein Genes ◽  
The Relationship ◽  
Selection Of

Дан анализ фракционного состава, и приведены основные модели молекулярной структуры мицелл казеина. Казеины являются фосфопротеинами и представляют коллоидный мицеллярный комплекс с фосфатом кальция и небольшим количеством других минералов. Внутренняя молекулярная структура мицелл казеина до конца не изучена, однако многие ученые согласны с моделью строения, в которой гидрофильная С-концевая часть κ -казеина находится на поверхности мицеллы, что обеспечивает стерическое и электростатическое отталкивание и предотвращает агрегацию мицелл. Фракция κ- казеина обладает широким полиморфизмом, однако на сегодняшний день наиболее распространенными являются аллельные варианты А и В, отличающиеся заменой аминокислот Thr157>Ile в аллельном варианте B. Данная генетическая модификация теряет один гликозидный центр, в результате чего повышается сыропригодность молока, но снижается его термоустойчивость. Молоко, несущее аллельный вариант В по гену CSN3, характеризуется более высоким содержанием белка, а аллельный вариант А влияет на увеличение надоев. Исследование взаимосвязи структуры мицелл казеина, полиморфных вариантов генов казеина и технологических свойств позволяет проводить селекцию коров для получения молока с требуемыми технологическими свойствами. The analysis of the fractional composition and the main models of the molecular structure of casein micelles are given. Being a phosphoprotein, casein is a colloid micellar complex with calcium phosphate and some other minerals. Inner molecule structure of casein’s micelle is not thoroughly studied. However, many scientists acknowledge one formation model, in which hydrophilic C-terminal part of kappa casein occupy micelle’s surface, thus providing steric and electrostatic repulsion and preventing micelles’ aggregation. Kappa casein fraction has a wide polymorphism, but the most common ones are A and B alleles with a distinctive replacement of amino acids Thr157>Ile in B allele. This genetic modification loses one glycoside center, resul- ting in milk’s cheeseability improvement, but also its heat resistance decrease. Moreover, milk with B allele of CSN3 has higher protein content, while A allele influences the increase in milk yield. The study of the relationship between the structure of casein micelles, polymorphic variants of casein genes and technological properties allows for the selection of cows to obtain milk with the required technological properties.

Evolution of Drosophila insecticide resistance

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 1-7 ◽  
Author(s):  
R. A. Morton
Keyword(s):  
Insecticide Resistance ◽  
Quantitative Traits ◽  
Molecular Genetic ◽  
Resistance Evolution ◽  
Target Molecules ◽  
Genetic Techniques ◽  
The Cost ◽  
The Impact ◽  
The Relationship ◽  
Selection Of

The impact of insecticide resistance is well documented. It includes the toxic effects of pesticides on the environment and the cost of the increased amounts of insecticides required to effectively control resistant insects. Resistance evolves by the selection of genes that confer tolerance to insecticides. Several resistance genes have been identified and cloned in Drosophila, including genes for mutant target molecules and genes that increase insecticide degradation. Drosophila is a useful system to understand the evolution of quantitative traits in general as well as the population genetics of insecticide resistance. Through it, we may hope to understand the relationship between discrete genetic change and continuously varying characters. In addition, molecular genetic techniques developed using Drosophila can eventually be transferred to other insects in order to help control pest populations.Key words: insecticide resistance, evolution of tolerance, selection of resistant genes, molecular genetics, Drosophila.

Thickness of endometrium: predictor of the effectiveness of IVF/ICSI programs (literature review)

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 113-116
Author(s):  
L A Bagdasaryan ◽  
I E Korneyeva
Keyword(s):  
Assisted Reproductive Technologies ◽  
Endometrial Thickness ◽  
Molecular Genetic ◽  
Uterine Cavity ◽  
Reproductive Technologies ◽  
Genetic Characteristics ◽  
Vitro Fertilization ◽  
Need To Evaluate ◽  
The Relationship

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.

IMPROVEMENT OF SPRING DURUM WHEAT SELECTION METHODOLOGIES UNDER CLIMATE CHANGE CONDITIONS

1970 ◽  
pp. 33-36
Author(s):  
А. I. Grabovets ◽  
V. P. Kadushkina ◽  
S. А. Kovalenko
Keyword(s):  
Grain Yield ◽  
Durum Wheat ◽  
Correlation Coefficient ◽  
Harvest Index ◽  
Unit Area ◽  
Correlation Coefficients ◽  
Chemical Mutagenesis ◽  
Main Method ◽  
The Relationship ◽  
Selection Of

With the growing aridity of the climate on the Don, it became necessary to improve the methodology for conducting the  breeding of spring durum wheat. The main method of obtaining the source material remains intraspecific step hybridization. Crossings were performed between genetically distant forms, differing in origin and required traits and properties. The use of chemical mutagenesis was a productive way to change the heredity of genotypes in terms of drought tolerance. When breeding for productivity, both in dry years of research and in favorable years, the most objective markers were identified — the size of the aerial mass, the mass of grain per plant, spike, and harvest index. The magnitude of the correlation coefficients between the yield per unit area and the elements of its structure is established. It was most closely associated with them in dry years, while in wet years it decreased. Power the correlation of the characteristics of the pair - the grain yield per square meter - the aboveground biomass averaged r = 0.73, and in dry years it was higher (0.91) than in favorable ones (0.61 - 0.70) , between the harvest and the harvest index - r = 0.81 (on average). In dry years, the correlation coefficient increased to 0.92. Research data confirms the greatest importance of the mass of grain from one ear and the plant in the formation of grain yield per unit area in both dry and wet years. In dry years, the correlation coefficient between yield and grain mass per plant was on average r = 0.80; in favorable years, r = 0.69. The relationship between yield and grain mass from the ear was greater — r = 0.84 and r = 0.82, respectively. Consequently, the breeding significance of the aboveground mass and the productivity of the ear, as a criterion for the selection of the crop, especially increases in the dry years. They were basic in the selection.

Analisa Pemilihan Moda Transportasi Umum Rute Tanjung Karang – Bandara Radin Inten II Dengan Stated Preference dan Uji Crame’s V

2018 ◽  
Vol 2 (2) ◽  
pp. 137
Author(s):  
Muhammad Abi Berkah Nadi
Keyword(s):  
Regression Analysis ◽  
Utility Function ◽  
Stated Preference ◽  
Technical Analysis ◽  
Chi Square ◽  
Time Headway ◽  
Independent Variable ◽  
The Relationship ◽  
Selection Of

Radin Inten II Airport is a national flight in Lampung Province. In this study using the technical analysis stated preference which is the approach by conveying the choice statement in the form of hypotheses to be assessed by the respondent. By using these techniques the researcher can fully control the hypothesized factors. To determine utility function for model forecasting in fulfilling request of traveler is used regression analysis with SPSS program. The analysis results obtained that the passengers of the dominant airport in the selection of modes of cost attributes than on other attributes. From the result of regression analysis, the influence of independent variable to the highest dependent variable is when the five attributes are used together with the R square value of 8.8%. The relationship between cost, time, headway, time acces and service with the selection of modes, the provision that states whether or not there is a decision. The significance of α = 0.05 with chi-square. And the result of Crame's V test average of 0.298 is around the middle, then the relationship is moderate enough.

A Biomarker Combining Imaging and Neuropsychological Assessment for Tracking Early Alzheimer's Disease in Clinical Trials

2018 ◽  
Vol 15 (5) ◽  
pp. 429-442 ◽  
Author(s):  
Nishant Verma ◽  
S. Natasha Beretvas ◽  
Belen Pascual ◽  
Joseph C. Masdeu ◽  
Mia K. Markey ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Clinical Trials ◽  
Cognitive Impairment ◽  
Latent Variable ◽  
Brain Regions ◽  
Disease Assessment ◽  
Latent Variable Analysis ◽  
The Relationship ◽  
Selection Of

Background: Combining optimized cognitive (Alzheimer's Disease Assessment Scale- Cognitive subscale, ADAS-Cog) and atrophy markers of Alzheimer's disease for tracking progression in clinical trials may provide greater sensitivity than currently used methods, which have yielded negative results in multiple recent trials. Furthermore, it is critical to clarify the relationship among the subcomponents yielded by cognitive and imaging testing, to address the symptomatic and anatomical variability of Alzheimer's disease. Method: Using latent variable analysis, we thoroughly investigated the relationship between cognitive impairment, as assessed on the ADAS-Cog, and cerebral atrophy. A biomarker was developed for Alzheimer's clinical trials that combines cognitive and atrophy markers. Results: Atrophy within specific brain regions was found to be closely related with impairment in cognitive domains of memory, language, and praxis. The proposed biomarker showed significantly better sensitivity in tracking progression of cognitive impairment than the ADAS-Cog in simulated trials and a real world problem. The biomarker also improved the selection of MCI patients (78.8±4.9% specificity at 80% sensitivity) that will evolve to Alzheimer's disease for clinical trials. Conclusion: The proposed biomarker provides a boost to the efficacy of clinical trials focused in the mild cognitive impairment (MCI) stage by significantly improving the sensitivity to detect treatment effects and improving the selection of MCI patients that will evolve to Alzheimer’s disease.

Excessive Belching: a Separate Syndrome or Another Symptom of Functional Dyspepsia?

2020 ◽  
Vol 30 (3) ◽  
pp. 55-60 ◽  
Author(s):  
A. A. Sheptulin ◽  
O. A. Storonova
Keyword(s):  
Functional Dyspepsia ◽  
Functional Gastrointestinal Disorders ◽  
High Resolution Manometry ◽  
Published Evidence ◽  
Key Points ◽  
Dyspeptic Symptoms ◽  
The Relationship ◽  
Rome Iv ◽  
Selection Of ◽  
Optimal Treatment Strategy

Aim of review. Interpretation of published evidence on the relationship between the excessive belching syndrome and functional dyspepsia (FD) and their management in patient care.Key points. According to the Rome IV criteria of functional gastrointestinal disorders (FGID), excessive belching in the absence of other dyspeptic symptoms is to be considered a manifestation of the excessive belching syndrome, which can be of gastric or supragastric nature. Combination of high-resolution manometry and impedancemetry allows an accurate diagnosis of belching and selection of optimal treatment strategy. Belching complicated by other symptoms of dyspepsia is to be considered yet another FD symptom according to the Rome IV criteria of FGID. Prokinetics are recommended to relieve belching in such cases.Conclusion. Understanding the relationship between the excessive belching syndrome and FD requires further research.

scholarly journals The Impact of Alkaloid-Producing Epichloë Endophyte on Forage Ryegrass Breeding: A New Zealand Perspective

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 158
Author(s):  
Colin Eady
Keyword(s):  
Seed Production ◽  
Health Issues ◽  
Abiotic Stressors ◽  
Livestock Health ◽  
The Right ◽  
The Impact ◽  
The Relationship ◽  
And Storage ◽  
Selection Of ◽  
Epichloë Endophyte

For 30 years, forage ryegrass breeding has known that the germplasm may contain a maternally inherited symbiotic Epichloë endophyte. These endophytes produce a suite of secondary alkaloid compounds, dependent upon strain. Many produce ergot and other alkaloids, which are associated with both insect deterrence and livestock health issues. The levels of alkaloids and other endophyte characteristics are influenced by strain, host germplasm, and environmental conditions. Some strains in the right host germplasm can confer an advantage over biotic and abiotic stressors, thus acting as a maternally inherited desirable ‘trait’. Through seed production, these mutualistic endophytes do not transmit into 100% of the crop seed and are less vigorous than the grass seed itself. This causes stability and longevity issues for seed production and storage should the ‘trait’ be desired in the germplasm. This makes understanding the precise nature of the relationship vitally important to the plant breeder. These Epichloë endophytes cannot be ‘bred’ in the conventional sense, as they are asexual. Instead, the breeder may modulate endophyte characteristics through selection of host germplasm, a sort of breeding by proxy. This article explores, from a forage seed company perspective, the issues that endophyte characteristics and breeding them by proxy have on ryegrass breeding, and outlines the methods used to assess the ‘trait’, and the application of these through the breeding, production, and deployment processes. Finally, this article investigates opportunities for enhancing the utilisation of alkaloid-producing endophytes within pastures, with a focus on balancing alkaloid levels to further enhance pest deterrence and improving livestock outcomes.

Selection of Materials and Sensors for Health Monitoring of Composite Structures

2003 ◽  
Vol 785 ◽  
Author(s):  
Seth S. Kessler ◽  
S. Mark Spearing
Keyword(s):  
Health Monitoring ◽  
Lamb Wave ◽  
Composite Structures ◽  
Structural Damage ◽  
Performance Metrics ◽  
Full Range ◽  
Transportation Systems ◽  
Health Monitoring Systems ◽  
The Relationship ◽  
Selection Of

ABSTRACTEmbedded structural health monitoring systems are envisioned to be an important component of future transportation systems. One of the key challenges in designing an SHM system is the choice of sensors, and a sensor layout, which can detect unambiguously relevant structural damage. This paper focuses on the relationship between sensors, the materials of which they are made, and their ability to detect structural damage. Sensor selection maps have been produced which plot the capabilities of the full range of available sensor types vs. the key performance metrics (power consumption, resolution, range, sensor size, coverage). This exercise resulted in the identification of piezoceramic Lamb wave transducers as the sensor of choice. Experimental results are presented for the detailed selection of piezoceramic materials to be used as Lamb wave transducers.

Decomposition of Fiscal Deterioration in the OECD

2010 ◽  
Vol 213 ◽  
pp. F13-F18 ◽  
Keyword(s):  
Financial Institutions ◽  
Government Support ◽  
Budget Deficits ◽  
Government Budget ◽  
Fiscal Stimulus ◽  
Advanced Economies ◽  
The Government ◽  
Cumulative Deterioration ◽  
The Relationship ◽  
Selection Of

Government fiscal positions in all the advanced economies suffered severe deteriorations during the financial crisis. Figure 1 illustrates the cumulative deterioration of the government budget ratio as a per cent of GDP between 2007 and 2009 in a selection of OECD economies. The sharpest declines materialised in Ireland, Spain and Finland, while public finances in Austria, Germany and Italy have held up better. Budget deficits have worsened in part because of the cyclical downturn, in part because of the policy response to the crisis, including both fiscal stimulus packages and certain fiscal costs related to government support of financial institutions, and in part because of a change in the relationship between revenue and production, which may prove longer-term.

Characterization and Selection of LAB Isolated from Inner Mongolia Traditional Meat Sausages

2012 ◽  
Vol 554-556 ◽  
pp. 1400-1405
Author(s):  
Yan Duan ◽  
Wen Rui Guo ◽  
Lin Su ◽  
Yue Ying Guo ◽  
Ye Jin
Keyword(s):  
Biogenic Amine ◽  
Inner Mongolia ◽  
Acid Production ◽  
Starter Culture ◽  
Technological Properties ◽  
Lactobacillus Curvatus ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Hydrolysis Of ◽  
Selection Of

A total of 69 gram-positive, catalase-negative strains isolated from Inner Mongolia traditional meat sausages were characterized in respect to their technological properties, including acid production, salt and nitrite tolerance, gas, slime and H2S production, hydrolysis of arginine and biogenic amine production. All of the isolated strains decrease the pH below 5.0 after 24h of cultivation. All isolates are able to grow with 6.5% NaCl or with 150mg L-1NaNO2and 51 isolates are able to grow with 10% NaCl. All isolates don’t produce gas and slime. 54 isolates don’t produce H2S. 23 isolated strains don’t produce NH3 from arginine, and 40 isolates don’t produce biogenic amine. Consolidation of these results, 8 strains show the best technological properties to be used as starter culture for sausage production. The bacterial selected are then subjected to biochemical tests using the API 50 CH and they are identified as 6 Lactobacillus planarum and 2 Lactobacillus curvatus.

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