scholarly journals Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam

2018 ◽  
Vol 2 (2) ◽  
pp. 119-125
Author(s):  
Esty Tyastirin ◽  
Risa Purnamasari ◽  
Irul Hidayati ◽  
Eva Agustina ◽  
Moch. Irfan Hadi ◽  
...  
Keyword(s):  
Mus Musculus ◽  
Laboratory Experiments ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Sucrose Induction ◽  
Cholesterol Levels ◽  
Randomized Design ◽  
Gaussian Data

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.

scholarly journals Pengaruh Ekstrak Rebusan Daun Tithonia diversifolia (Hemsl.) A. Gray Terhadap Kadar Glukosa Darah

2019 ◽  
Vol 7 (1) ◽  
pp. 41
Author(s):  
Rinawati Rinawati ◽  
E Suharyanto ◽  
Nastiti Wijayanti
Keyword(s):  
Blood Glucose ◽  
Young Leaf ◽  
Tithonia Diversifolia ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Adult Leaf ◽  
Reduce Blood Glucose ◽  
Completely Randomized Design

Tithonia diversifolia is a plant that has the potential to reduce blood glucose levels that is crucial in Diabetes Mellitus therapy. This study aims to determine the active compounds contained in the extract of T. diversifolia leaves decoction, the effect of decoction extract on the reduction of blood glucose, and the most effective decoction extract to reduce blood glucose levels as well. The study used a Completely Randomized Design (CRD) with the research treatment groups, namely: Kn = normal control (normal mice and not given leaves decoction extract), Ka = STZ control (DM mice, which are normal mice induced by 65mg/Kg of STZ), Kp = treatment control (normal mice given leaf decoction extract) , P1 = DM + mice were given young leaf decoction extract, P2 = DM + mice were given a mixture of young leaf and adult leaf decoction extract, P3 = DM + mice were given adult leaf decoction extract. The leaves used for decoction were 1-6 leaves from the shoots. The analysis of T. diversifolia leaves decoction compounds is using the visible spectrophotometers for tannins, phenols and flavonoids and GC-MS for terpenoid analysis. The result found that Tithonia diversifolia leaves decoction extracts contain tannins, flavonoids and phenols, while terpenoids are not detected. The leaves decoction extracts of T. diversifolia influenced the decreases in blood glucose levels of DM mice, especially the decoction extract of adult leaves which reduced blood glucose levels to 71.16%.

scholarly journals Teucrium polium: Potential Drug Source for Type 2 Diabetes Mellitus

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 128
Author(s):  
Yaser Albadr ◽  
Andrew Crowe ◽  
Rima Caccetta
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Insulin Secretion ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
Teucrium Polium

The prevalence of type 2 diabetes mellitus is rising globally and this disease is proposed to be the next pandemic after COVID-19. Although the cause of type 2 diabetes mellitus is unknown, it is believed to involve a complex array of genetic defects that affect metabolic pathways which eventually lead to hyperglycaemia. This hyperglycaemia arises from an inability of the insulin-sensitive cells to sufficiently respond to the secreted insulin, which eventually results in the inadequate secretion of insulin from pancreatic β-cells. Several treatments, utilising a variety of mechanisms, are available for type 2 diabetes mellitus. However, more medications are needed to assist with the optimal management of the different stages of the disease in patients of varying ages with the diverse combinations of other medications co-administered. Throughout modern history, some lead constituents from ancient medicinal plants have been investigated extensively and helped in developing synthetic antidiabetic drugs, such as metformin. Teucrium polium L. (Tp) is a herb that has a folk reputation for its antidiabetic potential. Previous studies indicate that Tp extracts significantly decrease blood glucose levels r and induce insulin secretion from pancreatic β-cells in vitro. Nonetheless, the constituent/s responsible for this action have not yet been elucidated. The effects appear to be, at least in part, attributable to the presence of selected flavonoids (apigenin, quercetin, and rutin). This review aims to examine the reported glucose-lowering effect of the herb, with a keen focus on insulin secretion, specifically related to type 2 diabetes mellitus. An analysis of the contribution of the key constituent flavonoids of Tp extracts will also be discussed.

scholarly journals Efek Serbuk Kunyit dan Kurkumin pada Spermatogenesis Mencit (Mus musculus) yang Diberi Minuman Beralkohol

2021 ◽  
Vol 6 (2) ◽  
pp. 154-160
Author(s):  
Helena Chika Valencia Hanisa ◽  
Tyas Rini Saraswati ◽  
Silvana Tana
Keyword(s):  
Analysis Of Variance ◽  
Mus Musculus ◽  
Alcoholic Beverages ◽  
Seminiferous Tubules ◽  
Treatment Groups ◽  
Alcohol Control ◽  
Randomized Design ◽  
Testicular Weight ◽  
Significant Difference ◽  
Spermatogonia Cells

Kunyit mengandung senyawa kurkumin yang dapat digunakan sebagai zat antiinflamasi dan membantu memperbaiki sel-sel yang rusak. Tujuan dari penelitian ini menganalisis pengaruh serbuk kunyit dan kurkumin pada jumlah dan ukuran sel spermatogonium; spermatosit primer; dan spermatosit sekunder; bobot testis serta diameter tubulus seminiferus Mus musculus yang diberi minuman beralkohol. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 12 ekor Mus musculus jantan yang dibagi kedalam 4 kelompok perlakuan dan 3 kali ulangan. R0 merupakan kontrol, R1 kontrol alkohol, R2 pemberian serbuk kunyit sebanyak 0,1 mg/hari, R3 pemberian kurkumin sebanyak 0,01 mg/hari. Perlakuan diberikan selama 30 hari. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa tidak terdapat perbedaan bermakna (p>0.05) pada jumlah spermatogonium dan ukuran sel (spermatogonium, spermatosit primer, dan spermatosit sekunder), namun terdapat perbedaan bermakna pada (P<0,05) pada bobot testis, diameter tubulus seminiferus dan jumlah sel (spermatosit primer, dan spermatosit sekunder). Turmeric contains curcumin compounds that can be used as anti-inflammatory substances and help repair damaged cells. The purpose of this study was to analyze the effect of turmeric powder and curcumin on the number and size of spermatogonia cells; primary spermatocytes; and secondary spermatocytes; testicular weight and diameter of the seminiferous tubules of Mus musculus given alcoholic beverages. This study is an experimental study with a completely randomized design (CRD), using 12 male Mus musculus which were divided into 4 treatment groups and 3 replications. R0 is control, R1 is alcohol control, R2 is 0.1 mg/day of turmeric powder, R3 is 0.01 mg/day of curcumin. The treatment was given for 30 days. The research data were analyzed using analysis of variance (ANOVA) at the 95% confidence level. Based on the results obtained, it can be concluded that there is no significant difference (p>0.05) in the number of spermatogonia and cell size (spermatogonia, primary spermatocytes, and secondary spermatocytes), but there is a significant difference (P<0.05) in testicular weight, diameter of the seminiferous tubules and the number of cells (primary spermatocytes, and secondary spermatocytes).

Protective effect of 4,6-O-ethylidene glucose against the cytotoxicity of streptozotocin in pancreatic β cells in vivo: indirect evidence for the presence of a glucose transporter in β cells

1987 ◽  
Vol 112 (3) ◽  
pp. 375-378 ◽  
Author(s):  
J. Kawada ◽  
M. Okita ◽  
M. Nishida ◽  
Y. Yoshimura ◽  
K. Toyooka ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Glucose Transporter ◽  
Indirect Evidence ◽  
Tolerance Test ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
In Vivo Experiments ◽  
Glucose Transport System

ABSTRACT Ethylidene glucose (4,6-O-ethylidene glucose; EG) is known to bind the outer surface of the glucose transporter in the membranes of human erythrocytes and other mammalian cells. If a glucose transport system is present on pancreatic β cells and recognizes the glucose moiety of streptozotocin (STZ), EG should protect β cells from the cytotoxicity of STZ when it is administered with STZ. This possibility was examined in in-vivo experiments in rats. When EG and STZ were injected into rats together the animals did not become diabetic, as judged by (1) their blood glucose levels, (2) response in a glucose-tolerance test, and (3) insulin secretion in response to feeding. These results suggest that there is a glucose transporter present in β cells and also the transport of streptozotocin into β cells through this system. J. Endocr. (1987) 112, 375–378

Cell-wide analysis of secretory granule dynamics in three dimensions in living pancreatic β-cells: evidence against a role for AMPK-dependent phosphorylation of KLC1 at Ser517/Ser520 in glucose-stimulated insulin granule movement

2010 ◽  
Vol 38 (1) ◽  
pp. 205-208 ◽  
Author(s):  
Angela McDonald ◽  
Sarah Fogarty ◽  
Isabelle Leclerc ◽  
Elaine V. Hill ◽  
D. Grahame Hardie ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Glutathione Transferase ◽  
Three Dimensions ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
Insulin Granules ◽  
Elevated Glucose ◽  
Glucose Stimulated Insulin Secretion

Glucose-stimulated insulin secretion from pancreatic β-cells requires the kinesin-1/Kif5B-mediated transport of insulin granules along microtubules. 5′-AMPK (5′-AMP-activated protein kinase) is a heterotrimeric serine/threonine kinase which is activated in β-cells at low glucose concentrations, but inhibited as glucose levels increase. Active AMPK blocks glucose-stimulated insulin secretion and the recruitment of insulin granules to the cell surface, suggesting motor proteins may be targets for this kinase. While both kinesin-1/Kif5B and KLC1 (kinesin light chain-1) contain consensus AMPK phosphorylation sites (Thr693 and Ser520, respectively) only recombinant GST (glutathione transferase)–KLC1 was phosphorylated by purified AMPK in vitro. To test the hypothesis that phosphorylation at this site may modulate kinesin-1-mediated granule movement, we developed an approach to study the dynamics of all the resolvable granules within a cell in three dimensions. This cell-wide approach revealed that the number of longer excursions (>10 μm) increased significantly in response to elevated glucose concentration (30 versus 3 mM) in control MIN6 β-cells. However, similar changes were seen in cells overexpressing wild-type KLC1, phosphomimetic (S517D/S520D) or non-phosphorylatable (S517A/S520A) mutants of KLC1. Thus, changes in the phosphorylation state of KLC1 at Ser517/Ser520 seem unlikely to affect motor function.

The Ins and Outs of Secretion from Pancreatic β-Cells: Control of Single-Vesicle Exo- and Endocytosis

Physiology ◽  
2007 ◽  
Vol 22 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Patrick E. MacDonald ◽  
Patrik Rorsman
Keyword(s):  
Plasma Membrane ◽  
Plasma Glucose ◽  
Secretory Vesicles ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
Single Vesicle

Exocytosis of insulin-containing secretory vesicles in pancreatic β-cells is crucial to maintenance of plasma glucose levels. They fuse with the plasma membrane in a regulated manner to release their contents and are subsequently recaptured either intact or through conventional clathrin-mediated endocytosis. Here, we discuss these mechanisms in β-cells at the single-vesicle level.

scholarly journals PEMANFAATAN INFUSA DAUN SIRIH MERAH (Piper crocatum) SEBAGAI ANTI-OBESITAS PADA MENCIT (Mus musculus)

2020 ◽  
Vol 10 ◽  
pp. 50-57
Author(s):  
Desty Apritya ◽  
Miarsono Sigit ◽  
Retina Yunani ◽  
Fuji Lestari
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Experimental Animals ◽  
Betel Leaf ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Obesity In Mice ◽  
Sugar Levels ◽  
The Relationship

The purpose of this research is to know the effectiveness of red betel leaf infusion (Piper crocatum) as anti-obesity in mice (Mus musculus). Other parameters of this study to know the relationship between changes in blood glucose levels to obesity in experimental animals. Experimental animals used were 24 male mice. The design of this research is completely randomized design (CRD) with 4 treatments 6 repetitions. There are four treatmens P0 (Control), P1 (6 mice by giving a betel leaf infusion with a concentration of 5%), P2 (6 mice by giving a betel leaf infusion with a concentration of 10%), and P3 (6 mice by giving a betel leaf infusion with a concentration of red 15%). The treatment is carried out once every day for 21 days. Before treatment, initial weight was weighed and blood glucose level examination continued on the 7th day, on the 14th day, and on the 21st day. Data obtained from the results of weighing and checking blood sugar levels were further analyzed using the ANOVA test which statistically showed no significant changes (P> 0.05) of body weight and blood glucose levels in mice (Mus musculus) given infusion red betel leaf (Piper crocatum).

scholarly journals The effect of exposure to liquid air freshener on histological of the bronchial mice (Mus musculus)

2021 ◽  
Vol 6 (02) ◽  
Author(s):  
Warobi Warobi ◽  
Delima Engga Maretha ◽  
Asnilawati Asnilawati ◽  
Mashuri Masri
Keyword(s):  
Mus Musculus ◽  
The Body ◽  
Control Group ◽  
Epithelial Tissue ◽  
Treatment Groups ◽  
Histological Picture ◽  
Randomized Design ◽  
Post Test ◽  
The One ◽  
Post Hoc

Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.

Emerging roles for the ubiquitin-proteasome system and autophagy in pancreatic β-cells

2009 ◽  
Vol 296 (1) ◽  
pp. E1-E10 ◽  
Author(s):  
Taila Hartley ◽  
John Brumell ◽  
Allen Volchuk
Keyword(s):  
Cell Function ◽  
Ubiquitin Proteasome System ◽  
Contributory Factor ◽  
Pancreatic Β Cell ◽  
Β Cells ◽  
Β Cell ◽  
Pancreatic Β Cells ◽  
Glucose Levels ◽  
Pathological Conditions ◽  
Ubiquitin Proteasome

Protein degradation in eukaryotic cells is mediated primarily by the ubiquitin-proteasome system and autophagy. Turnover of protein aggregates and other cytoplasmic components, including organelles, is another function attributed to autophagy. The ubiquitin-proteasome system and autophagy are essential for normal cell function but under certain pathological conditions can be overwhelmed, which can lead to adverse effects in cells. In this review we will focus primarily on the insulin-producing pancreatic β-cell. Pancreatic β-cells respond to glucose levels by both producing and secreting insulin. The inability of β-cells to secrete sufficient insulin is a major contributory factor in the development of type 2 diabetes. The aim of this review is to examine some of the crucial roles of the ubiquitin-proteasome system and autophagy in normal pancreatic β-cell function and how these pathways may become dysfunctional under pathological conditions associated with metabolic syndromes.

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