scholarly journals Investigation of Two Precipitation Methods for Extracting Immunoglobulin Y (IgY) from Egg Yolks

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Tran Thi Quynh Lan ◽  
Tran Trong Kha
Keyword(s):  
Protein Profile ◽  
Extraction Process ◽  
Control Group ◽  
Standard Curve ◽  
Immunization Group ◽  
Sds Page ◽  
Precipitation Processes ◽  
Egg Yolks ◽  
Yellow Pigments ◽  
And Control

Two groups of hens (control and immunization group) were arranged in an experimental design with an immunization schedule of 3 injections of BSA antigen. IgY antibodies were extracted from egg yolks by two precipitation processes (chloroform and polyethylene glycol precipitates) and quantified using a standard curve of protein concentration. The purification of IgYwas confirmed by SDS-PAGE. Total protein extracted from egg yoks were less contaminated with yellow pigments (lutein and zeaxanthin) by using chloroform precipitate. The 2nd week post-immunization, IgY concentration increased respectively to 3903 ± 726 μg.ml-1 (chloroform extraction process) and 2937 ± 294 μg.ml-1 (PEG extraction process) (P < 0.01). After 3rdimmunization, IgY level obtaining from in immunization group extracted by chloroform process (6633 ± 1166 μg.ml-1) increased 2.7 times higher than that in control group (2482 ± 414 μg.ml-1). Whereas IgY concentrations obtained from PEG extraction process were not significantly different between the experimental group and control group. Chloroform and PEG precipitation methods had the same protein profile on the SDSPAGE. IgY antibody was identified by the presence of bands corresponding with IgY heavy chain (67-70 kDa) and IgY light chain (25 kDa) for both precipitation processes.

scholarly journals Measurement of serum and peritoneal fluid LH concentrations as a diagnostic tool for human endometriosis

Reproduction ◽  
2001 ◽  
pp. 761-769 ◽  
Author(s):  
JC Illera ◽  
G Silvan ◽  
MJ Illera ◽  
CJ Munro ◽  
BA Lessey ◽  
...  
Keyword(s):  
Predictive Value ◽  
Peritoneal Fluid ◽  
Polyclonal Antiserum ◽  
Control Group ◽  
Standard Curve ◽  
Serum Lh ◽  
Assay Time ◽  
Cycle Dependent ◽  
And Control ◽  
One Way Anova

A rapid, sensitive enzymeimmunoassay for the measurement of LH concentrations in serum and peritoneal fluid samples of healthy women and women with endometriosis is reported. The ligand (LH) was captured by a readily available, widely used and well-characterized monoclonal antibody (mAb, 518B7) generated against the beta subunit of bovine LH. This mAb, although specific for LH, shows very little species specificity and detects LH by radioimmunoassay in humans. A polyclonal antiserum raised in rabbits against hCG was conjugated to horseradish peroxidase and was used as the second antibody signal. This anti-hCG antiserum crossreacts with LH. The enzymeimmunoassay uses the standard human LH (hLH) preparations (NIADDK-hLH-I-3, AFP-827OB) and results are based on the relative concentrations of LH in serum and peritoneal fluid. Total assay time was < 3 h. The range of the standard curve was 0.002-0.500 ng LH per well and the lowest concentration of hLH that could be distinguished from zero concentration was 0.15 +/- 0.02 ng ml(-1) serum and 0.058 +/- 0.021 ng ml(-1) peritoneal fluid. Clinical diagnostic parameters for the LH enzymeimmunoassay showed a sensitivity of 85.71%, specificity 92.50%, efficiency 88.54%, positive predictive value 94.11% and negative predictive value 82.22%. The study was retrospective. Serum LH concentrations of women with endometriosis were 13.67 +/- 7.21 ng ml(-1), whereas serum LH concentrations of women in the control group were 4.52 +/- 2.03 ng ml(-1). One-way ANOVA showed significant differences (P < 0.001) between women with endometriosis and control groups. Women in the control group had peritoneal fluid LH values of 5.65 +/- 2.43 ng ml(-1), whereas peritoneal fluid LH values of 64.06 +/- 16.44 ng ml(-1) were obtained in women with endometriosis (P < 0.001). A cycle-dependent pattern of serum and peritoneal fluid LH concentration was observed in women in the control group, which was not observed in the peritoneal fluid of the group with endometriosis. The application of this assay to serum or peritoneal fluid samples provides the attractive possibility that it could be included in the panel of markers used for diagnosis of endometriosis.

scholarly journals Impact of Meningococcal B (4CMenB) Vaccine on Pharyngeal Neisseria meningitidis Carriage Density and Persistence in Adolescents

2020 ◽  
Author(s):  
Mark McMillan ◽  
Luke Walters ◽  
Thomas Sullivan ◽  
Lex E X Leong ◽  
Mark Turra ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Quantitative Polymerase Chain Reaction ◽  
Cluster Randomized Trial ◽  
Control Group ◽  
Population Exposure ◽  
Standard Curve ◽  
Cluster Randomized ◽  
4Cmenb Vaccine ◽  
And Control ◽  
The Impact

Abstract Background Higher density of Neisseria meningitidis carriage may be associated with transmission of the meningococcus. Our aim was to establish the impact of meningococcal B (4CMenB) vaccine on N. meningitidis carriage density. Methods We compared 4CMenB vaccine to control among 913 South Australian students aged approximately 15–18 years in a cluster randomized trial who had N. meningitidis carriage at 12 months. Oropharyngeal swabs were collected at baseline and 12 months later to detect N. meningitidis carriage. Colony-forming units per milliliter (CFU/mL) were estimated by generating a standard curve that plotted quantitative polymerase chain reaction cycle threshold values against log-normalized CFU. Results Among the 913 students with N. meningitidis carriage at 12 months, there was no difference in mean carriage density between the vaccinated (n = 434; 3.80 log CFU/mL [standard deviation {SD}, 1.29]) and control group (n = 479; 3.73 log CFU/mL [SD, 1.30]; P = .51). Higher N. meningitidis carriage density at baseline was associated with an increase in the odds of persistent carriage at 12 months (n = 504; odds ratio [OR] per 1.0 log CFU/mL increase in density, 1.36 [95% confidence interval {CI}, 1.17–1.58]; P &lt; .001). Students with baseline carriage who were vaccinated had decreased persistent N. meningitidis carriage at 12 months compared to unvaccinated students (81/260 [31%] vs 105/244 [43%]; OR, 0.60 [95% CI, .40–.90]; P = .01). Conclusions 4CMenB vaccine did not reduce carriage density of N. meningitidis 12 months postvaccination, despite increased carriage clearance. Higher carriage density is likely to enable transmission through prolonged periods of population exposure. Clinical Trials Registration NCT03089086.

To compare the impact of sitting and supine position on behavioral distress during immunization among children (1-3 years) in selected immunization clinics

2021 ◽  
pp. 351-358
Author(s):  
Grace Kurian ◽  
Theresa Leonilda Mendonca
Keyword(s):  
Supine Position ◽  
Control Group ◽  
Sitting Position ◽  
Immunization Group ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
And Control ◽  
The Impact ◽  
Experimental Group

Background of the study: Immunizations cause distress in children due to its acute pain. Younger children are particularly in need of intervention because they report more pain and display more behavioral distress during painful procedures. One of the effective non-pharmacological interventions of acute management is comfort position given by the parent. Comfort position provided by the parent during immunizations has been demonstrated to be useful in infants, toddlers and pre-school children. Yet, this simple intervention is not used on a routine basis. Aim: The aim of the study was to compare the impact of sitting and supine position on behavioral distress during immunization among children (1-3 years) in selected immunization clinics. Objectives of the study: To determine the impact of sitting position on behavioral distress of children receiving immunization (Group I - experimental group). 1. To determine the impact of supine position on behavioral distress of children receiving immunization (Group II - control group). 2. To compare the impact of sitting and supine position on behavioral distress of children during immunization. Methods: The research design adopted for the study was post test only control group design. Immunization clinic selected based on the convenience of the investigator. Purposive sampling technique used to select the sample and the sample were assigned randomly in to Group I(experimental group) and Group II(control group).To assess the impact of position: PBRS-R was used to assess the behavioral distress of children during immunization injection. Results: The collected data was analyzed by descriptive and inferential statistics. 1. Assessment of behavioral distress scores during immunization injection showed significant difference in mean scores in Group I (16.4±2.30) and in Group II (28.45±2.59). 2. Comparison of behavioral distress scores in Group I and Group II showed that there is a significant difference (p<0.05) in behavioral distress between experimental (Group I) and control (Group II) group. 3. Item wise comparison of behavioral distress scores in Group I and Group II showed that there is no significant difference(p<0.05) in behavioral distress between experimental (Group I) and control (Group II) group. Interpretation and conclusion: Findings of the study revealed that the comfort position, i.e., sitting position was effective in reducing behavioral distress during immunisation. Hence, paediatric nurses ought to promote the use of comfort position to reduce behavioral distress associated with painful procedure.

scholarly journals Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System

2018 ◽  
Vol 54 (2) ◽  
pp. 146
Author(s):  
Sukarjati Sukarjati ◽  
Susie Amilah ◽  
Sudjarwo Sudjarwo
Keyword(s):  
Escherichia Coli ◽  
Dna Damage ◽  
Reproductive System ◽  
Assay Method ◽  
Control Group ◽  
Infertile Male ◽  
Infection Group ◽  
E Coli ◽  
Immunization Group ◽  
And Control

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

TEACHING READING BY UTILIZING HOT POTATOES SOFTWARE TOWARD STUDENTS’ READING COMPREHENSION OF DESCRIPTIVE TEXT

2018 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Abdullah Farih
Keyword(s):  
Reading Comprehension ◽  
Alternative Hypothesis ◽  
T Test ◽  
Control Group ◽  
Teaching Reading ◽  
Post Test ◽  
Quasi Experimental ◽  
Independent Variable ◽  
Level Of Significance ◽  
And Control

The objective of this study is to measure whether there is or not significant effect of teaching reading by utilizing Hot Potatoes software toward students’ reading comprehension of descriptive text. The variables used were the teaching treatment of Hot Potatoes Software as the independent variable and the students’ reading comprehension of Descriptive Text as dependent variable. Then, the research designed was Quasi-experimental design and the presentation of data used was quantitative. The data were obtained from the eleventh grade, of which 28 students were taken as sample. The students are divided into two groups; experiment group consisted of 12 students and control group consists of 16 students. To get the data, the pre-test and post-test were applied and then analyzed using t-test formula. The researcher had concluded that there is Significant effect of Hot Potatoes Software toward the students’ reading comprehension achievement. The result showed that mean of students’ post-test was increased. It is proved by the t-test (8.54) which is higher than t-table (2.05) at level of significance 5%. It means that the alternative hypothesis was accepted and it proved that Hot Potatoes Software had significant effect toward students’ reading comprehension of Descriptive Text

Perbandingan Efek Suplementasi Tablet Tambah Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Ibu Hamil Dengan Usiakehamilan 16-32 Minggu Di Desa Keniten Kecamatan Mojo Kabupaten Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 76
Author(s):  
Siti Asiyah ◽  
Dwi Estuning Rahayu ◽  
Wiranti Dwi Novita Isnaeni
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Gestational Age ◽  
Group Treatment ◽  
Sampling Technique ◽  
Hemoglobin Level ◽  
The Body ◽  
Control Group ◽  
Fetal Organ ◽  
And Control

The needed of Iron Tablet in pregnancy was increase than mother who not pregnant.  That  cause of  high metabolism at the pregnancy for formed of  fetal organ and energy. One of effort for prevent anemia in mother pregnant with giving the Iron tablet and vitamin c. The reason of  this research in 4 June – 11 July 2014 is for compare the effect of  iron tablet suplementation with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks In Desa Keniten Kecamatan Mojo Kabupaten Kediri. This research method using comparative analytical.  Research design type of Quasy Eksperiment that have treatment group and control group. Treatment group will giving by Iron tablet and 100 mg vitamin C, and control group just giving by iron tablet during 21 days. Population in this research are all of mother pregnant with Gestational Age Of 16-32 Weeks with Sampling technique is  cluster random sampling is 29 mother pregnant. Comparison analysis of  iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks, data analysis using Mann Whitney U-test and the calculated U value (44,5) less than U-table (51). So there was difference of iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks Therefore, the addition of vitamin C on iron intake is needed to increase the uptake of iron tablets. When the amount of iron uptake increases, the reserves of iron in the body will also increase, so as to prevent anemia in pregnant women; Keywords : Iron Tablet (Fe), Vitamin C, Hemoglobin level, Mother Pregnant

PENGARUH PENDEKATAN APTITUDE TREATMENT INTERACTION (ATI) TERHADAP PEMAHAMAN KONSEP MATEMATIKA SISWA KELAS VIII SMP NEGERI 25 PEKANBARU

2015 ◽  
Vol 4 (1) ◽  
pp. 79
Author(s):  
Herlina '
Keyword(s):  
Control Group ◽  
Treatment Interaction ◽  
Aptitude Treatment Interaction ◽  
Research Experiment ◽  
And Control

This research intent to see how big influence of approaching aptitude treatment interaction (ATI) to mathematics concept grasp student brazes VIII SMP Country 25 Pekanbaru. This research constitute my research experiment attention. Subjec in observational it is student braze VIII4 as agglomerate as experiment by totals student 40 person and VIII3'S classes as agglomerate as controls by totals students 40. Base analisis data to pretes's score to know student startup ability on agglomerate experiment and control group. On student experiment group that will study by ATI'S approaching has average early learned result mathematics (pretes) as big as 17,15. Meanwhile on group controls student who will study by ordinary learning (conventional) have average early learned result mathematics (pretes) as big as 13,85. Analisis is data to postes's score on agglomerate learned student experiment with ATI'S approaching has average final learned result mathematics (postes) as big as 74,63. Meanwhile on group controls learned student with ordinary learning (conventional) have average final learned result mathematics (postes) as big as 62,93. Of quiz result distinctive both of average usufruct to study mathematics finals (postes) that points out that there is difference which signifikan among both of experiment class with control class.Keywords: aptitude treatment interaction (ATI), mathematics concept

Non Emergency Calls-Depression Coupling in Call Handlers of Rescue 1122 Punjab, Pakistan

2019 ◽  
Vol 34 (Spring 2019) ◽  
pp. 43-55
Author(s):  
Syed Kamal Abid ◽  
Mujahid Hussain ◽  
Muhammad Raza ◽  
Rizwan-ul Haq ◽  
Rizwan Naseer ◽  
...  
Keyword(s):  
Occupational Stress ◽  
Independent Predictor ◽  
Depression Score ◽  
Demographic Variables ◽  
Workplace Stress ◽  
Control Group ◽  
Control Room ◽  
Emergency Calls ◽  
And Control ◽  
Evening Shift

The study was conducted to know whether bulk inflow of Non Emergency Calls (NECs) acts as an independent predictor for depression in call handlers of Rescue 1122, Punjab, Pakistan. Forty five (45) call handlers were recruited from evening shift of 9 districts. Similarly, same-sized control group was made out of field rescuers. The groups were compared for rate and severity level of depression using Beck’s Depression Inventory-II (BDI-II; Beck, Steer, & Brown, 1996). Probable predictors (except workplace stress) for depression in call handlers were evaluated through risk estimates. For workplace stress, a purposefully developed close- ended Workplace Stress Questionnaire including two subscales i.e. Non Emergency call and Control Room Environment of 30 items each was administrated. Twenty nine (64.4%) subjects of study group reported depression on BDI. Consequently, the same group had significantly higher mean depression score than control (18.2 vs. 12.6; p = .00). The scores also showed insignificant association with any of the probable predictors (demographic variables) of the call attendees. The respondents perceived more occupational stress against NECs. The findings attract the attention of authorities towards the severity of the concern.

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