scholarly journals Moderate Intensity Training By Listening To Music Decreases Interleukin-6 Levels In Rats

2020 ◽  
Vol 9 (1) ◽  
pp. 93-101
Author(s):  
Septyaningrum Putri Purwoto ◽  
Adi Pranoto ◽  
Hartono Hartono ◽  
Sugiharto Sugiharto
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Moderate Intensity ◽  
Control Group ◽  
Elisa Method ◽  
Analysis Techniques ◽  
High Intensity Training ◽  
Randomized Control ◽  
Post Hoc ◽  
Intensity Training

The design of this study is the randomized control group posttest-only design, 21 of Rattus Norvegicus Strain Wistar male, 20-24 weeks, 250 ± 350 grams and randomly divided into 3 groups namely CONT (n = 7, control without treatment), MIT (n = 7, moderate intensity training, treadmill with speed 14-16 m/min for 15-30 minutes listening to music tempo 160 beats/min) and HIT (n = 7, high intensity training, treadmill with speed 22-25 m/min for 15-30 minutes listening to music tempo 160 beats/min). The intervention was started from 17.00-20.00 WIB with a frequency of 3 times / week for 10 weeks. Blood is drawn for 12 hours after the last training. Measurement of IL-6 serum levels using the ELISA method. Data analysis techniques using ANOVA test and LSD post hoc test with the Statistical Package for Social Science (SPSS).

scholarly journals The relationship between the expression of PLIN3 and PLIN5 protein flowing endurance training in streptozotocin rats

2019 ◽  
Vol 21 (4) ◽  
pp. 194-199
Author(s):  
Mahdi Ghafari ◽  
Ebrahim Banitalebi ◽  
Mohamad Faramarzi
Keyword(s):  
Insulin Resistance ◽  
Endurance Training ◽  
High Intensity ◽  
Training Group ◽  
Control Group ◽  
High Intensity Training ◽  
Significant Difference ◽  
Post Hoc ◽  
The Relationship ◽  
Intensity Training

Background and aims: Intermuscular lipolysis disorder plays an important role in insulin resistance and diabetes mellitus and perilipin PLIN5 and PLIN3 are the key proteins in regulating muscle cellular lipolysis. Therefore, the purpose of this study was to examine the relationship between the expression of PLIN3 and PLIN5 protein following endurance training in streptozotocin (STZ) rats. Methods: A number of 24 male Wistar rats were randomly divided into low endurance training group (n = 8), high-intensity training group (n = 8), and control group (n = 8). Diabetes was induced in every rat by STZ injection. Three days after injection, the blood samples were taken from the cut tip of the tails of the mice and animals with blood glucose greater than 300 mg/dL were considered diabetic. The training program included eight weeks of aerobic training at different intensities. Training in high- and low-intensity groups included 22-25 and 5-8 m/min of training. Finally, one-way analysis of variance (ANOVA) and correlation was used to determine the significance of the differences between variables, followed by utilizing Tukey’s post-hoc test for significance. Results: The comparison between the groups by ANOVA showed significant differences in PLIN3 (P=0.0006) and PLIN5 (P=0.012). The results of Tukey post hoc test also demonstrated a statistical difference between the mean values of diabetic control group and high-intensity endurance group regarding PLIN3 (P=0.01) and PLIN5 (P=0.009), but no significant increase was observed in the lowintensity exercise group as compared to the control group (PLIN3, P=0.067 & PLIN5, P=0.44). As regards insulin resistance, there was a significant difference among the three groups (P=0.0001). Eventually, the result of the correlation between PLIN3 and PLIN5 showed similar enhancement by increasing the intensity (P=0.0026). Conclusion: According to research results, high-intensity endurance training increased the expression of PLIN3 and PLIN5 in diabetic specimens and PLIN3 and PLIN5 followed a similar increase pattern in high-intensity training

scholarly journals PENGARUH GELATIN IKAN PATIN (PANGASIUS DJAMBAL) TERHADAP JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS PUTIH (RATTUS NORVEGICUS)

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Fredy Mardiyantoro ◽  
Fidya Fidya ◽  
Dena Savira Andriani
Keyword(s):  
Rattus Norvegicus ◽  
Tooth Extraction ◽  
Control Group ◽  
Fish Gelatin ◽  
Tukey Test ◽  
White Rats ◽  
Significant Difference ◽  
Post Hoc ◽  
Hematoxylin Eosin ◽  
Test Result

Background: Gelatin has been used as a hemostatic agent to stop bleeding and it can be absorbed by human body within 6 hours. The patin fish (Pangasius djambal) gelatin has a high amount of amino acid, especially glutamine which has an important role in increasing the proliferation of fibroblast in wounds. The purpose of this study was to determine the effect of patin fish gelatin to the number of fibroblast in wound after tooth extraction.Method: Rat’s tooth extraction is done on the lower left incisive tooth. Thirty white rats were divided into two groups randomly with 3 time series. Control group was the rats without given of patin fish gelatin and treatment group was the rats given 1cc of patin fish gelatin with pipette. Socket taken for histologically processed by Hematoxylin Eosin staining.Result: It showed there were significant differences in the number of fibroblasts between groups. The Post Hoc Tukey test result showed there was a significant difference between the control group and the treatment group.Conclusion: Patin fish gelatin has effect increasing the number of fibroblast inwound after white rat’s (Rattus norvegicus) tooth extraction

Comparison of High-Intensity Training Versus Moderate-Intensity Continuous Training on Cardiorespiratory Fitness and Body Fat Percentage in Persons With Overweight or Obesity: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

2021 ◽  
pp. 1-14
Author(s):  
Nivash Rugbeer ◽  
Demitri Constantinou ◽  
Georgia Torres
Keyword(s):  
Body Fat ◽  
High Intensity ◽  
Mean Difference ◽  
Moderate Intensity ◽  
Body Fat Percentage ◽  
Fat Percentage ◽  
Continuous Training ◽  
High Intensity Training ◽  
Significant Difference ◽  
Intensity Training

Background: High-intensity training is comprised of sprint interval training (SIT) and high-intensity interval training (HIIT). This study compared high-intensity training with moderate-intensity continuous training (MICT) on cardiorespiratory fitness (CRF) and body fat percentage for overweight or obese persons. Methods: A systematic search of randomized controlled trials using the health science databases occurred up to April, 2020. Twenty-six studies were included for complete analysis. A total of 784 participations were analyzed. The unstandardized mean difference for each outcome measurement was extracted from the studies and pooled with the random effects model. Results: MICT was significantly better at improving CRF compared with SIT (mean difference = −0.92; 95% confidence interval, −1.63 to −0.21; P = .01; I2 = 10%). Furthermore, there was no significant difference between MICT versus HIIT on CRF (mean difference = −0.52; 95% confidence interval, −1.18 to 0.13; P = .12; I2 = 23%). There was no significant difference in body fat percentage between MICT versus HIIT and MICT versus SIT. Conclusions: MICT was significantly better at improving CRF than SIT in overweight or obese persons.

scholarly journals High Intensity Resistance Training Methods with and without Protein Supplementation to Fight Cardiometabolic Risk in Middle-Aged Males: A Randomized Controlled Trial

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Wolfgang Kemmler ◽  
Andreas Wittke ◽  
Michael Bebenek ◽  
Michael Fröhlich ◽  
Simon von Stengel
Keyword(s):  
Risk Factors ◽  
Resistance Training ◽  
High Intensity ◽  
Cardiometabolic Risk ◽  
High Volume ◽  
Protein Supplementation ◽  
Cardiometabolic Risk Factors ◽  
Control Group ◽  
High Intensity Training ◽  
Intensity Training

Time-effective protocols may potentially increase people’s compliance with exercise. The purpose of this paper was to compare the relative effects of 16 weeks of high intensity (resistance) training (HIT) with and without protein supplementation (HIT&P) and HVHIT (high volume/high intensity training) versus a nontraining control group on cardiometabolic risk factors. One hundred and twenty untrained males 30–50 years old were randomly assigned to 3 subgroups: (a) a HIT group; (b) a HIT&P group, and (c) a waiting-control group (phase I) that crossed over to (d) high volume/high intensity training (HVHIT) during the second study phase. HIT was defined as “single set to failure protocol” while HVHIT consistently applied two sets. Protein supplementation provided an overall intake of 1.5 g/kg/body mass. Primary study endpoint was the metabolic syndromeZ-Score (MetS-Z-Score). MetS-Z-Score significantly improved in all exercise groups (p≤0.001) with no significant difference between HIT, HIT&P, and HVHIT (p≥0.829). However, all the exercise groups differed significantly from the CG (p<0.001) which deteriorated significantly (p=0.039). In conclusion, all exercise protocols were similarly effective in improving cardiometabolic risk factors. Thus, HIT may be the best choice for people with low time budgets looking to improve their cardiometabolic health.

scholarly journals IMMUNE FUNCTION OF ZHENQI FUZHENG CAPSULE ON VOLLEYBALL PLAYERS AFTER HIGH INTENSITY TRAINING

2021 ◽  
Vol 27 (spe2) ◽  
pp. 66-69
Author(s):  
Lili Wang ◽  
Hongfei Wang ◽  
Wanliang Tuo
Keyword(s):  
Immune Function ◽  
High Intensity ◽  
White Blood Cells ◽  
Experimental Comparison ◽  
Control Group ◽  
Hemoglobin Content ◽  
Cell Content ◽  
Exercise Time ◽  
High Intensity Training ◽  
Intensity Training

ABSTRACT At present, many studies have proved that proper exercise can promote the immune function of human body to a certain extent, but athletes need a lot of high-intensity sports training, and their immune function declines instead of improving. In order to control the decline of immune function of athletes after high-intensity training, this study propose the Zhenqi Fuzheng capsule to achieve this goal. Through experimental comparison, the parameters such as white blood cell content, immunoglobulin number, T lymphocyte, human hemoglobin content and exercise exhaustion time were detected after high-intensity training. The results showed that compared with the control group taking Zhenqi Fuzheng, the weight of those who had taken qifuzhengs capsule did not change, and the content of white blood cells, immunoglobulin, hemoglobin content and exercise time increased to a certain extent. The results showed that Zhenqi Fuzheng could inhibit the decrease of body immune function after high-intensity exercise, then accelerate the recovery of human immune function. This study is expected to enhance the immunity of sports athletes, and reduce athletes’ pain after high-intensity training.

scholarly journals PENGARUH PEMBERIAN KONTRAS MEDIA NON IONIK LOW OSMOLAR INTRA VENA TERHADAP KADAR CYSTATIN–C SERUM PADA ORYCTALAGUS CUNICULUS

1970 ◽  
Vol 15 (2) ◽  
Author(s):  
Ariyo Sakso Bintoro ◽  
Soetojo Soetojo ◽  
Doddy M Soebadi
Keyword(s):  
Cystatin C ◽  
Sandwich Elisa ◽  
Intervention Group ◽  
Serum Levels ◽  
Control Group ◽  
Test Results ◽  
Elisa Method ◽  
Serum Cystatin C ◽  
Serum Cystatin ◽  
Ionic Contrast

Objective: To measure effects of low osmolar non ionic contrast media on cystatin–C serum levels on different days after administration. Material and Method: Twenty-two oryctalagus cuniculus were divided into two groups of 11 subjects, each subject receiving intravenous injection of low osmolar non ionic contrast or a placebo solution (NaCl 0,9%). Cystatin-C serum levels of each subject were measured before injection, on days 1, 3, 7, and 10. Cystatin–C serum levels were determined with a sandwich ELISA method. Statistical analysis was performed with t–test. Results: Mean cystatin–C serum levels before injection was 0,00337 ± 0,00101 mg/L. Means from days 1, 3, 7, and 10 after injections were 0,00498 ± 0,00153 mg/L; 0,00565 ± 0,00247 mg/L; 0,00468 ± 0,00157, and 0,00339 ± 0,00188 mg/L respectively. Conclusion: Increase in serum cystatin–C levels on days 1, 3, and 7 was significant. On the 10th day no significant escalation was observed. Compared to the control group, there were significant differences in serum cystatin–C increase on days 1 and 3 in the intervention group, but no significant differences 7 and 10 days after injection.

scholarly journals The Reasearch on the Anti-Fatigue Effect of Whey Protein Powder in Basketball Training

2015 ◽  
Vol 9 (1) ◽  
pp. 330-334 ◽  
Author(s):  
Sun Ronghui
Keyword(s):  
Whey Protein ◽  
Cycle Ergometer ◽  
Control Group ◽  
Blood Cell Count ◽  
Hematological Indices ◽  
Fatigue Effect ◽  
High Intensity Training ◽  
Red Blood Cell Count ◽  
Improve Exercise Capacity ◽  
Intensity Training

In order to observe the effects of whey protein powder on hematological indexes of players majoring in physical education in the basketball training, the authors divided the players randomly into a control group and a nutrition group. Athletes complete the 30 minutes quantitative exercise using cycle ergometer respectively before the trial and after one month trial. Then we exsanguinated immediately after exercise, extracted heparin and measured hemoglobin, red blood cell count, hematocrit and mean corpuscular volume and other hematological indices. The results showed that after taking whey protein powder, the HB, RBC, HCT of nutrition group was significantly higher that the control group. This suggests that in high-intensity training, taking whey protein powder can cause changes of HB, RBC and HCT in human body, meanwhile MCV essentially the same. So whey protein powder can improve exercise capacity, and has anti-fatigue effect.

scholarly journals PENGARUH PEMBERIAN EKSTRAK DAUN KELOR (Moringa oleifera) TERHADAP KADAR HEMOGLOBIN PADA TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR JANTAN

2021 ◽  
Vol 5 (2) ◽  
pp. 91-101
Author(s):  
Yesi Nurmalasari ◽  
Rakhmi Rafie ◽  
Efrida Warganegara ◽  
Lingga Desta Wahyuni
Keyword(s):  
Rattus Norvegicus ◽  
Moringa Oleifera ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Hoc ◽  
True Experiment ◽  
Paired T Test

Hemoglobin merupakan suatu protein tetrametrik eritrosit yang tersusun dari protein globin dan heme. Radikal bebas dapat menyebabkan lisisnya membran eritrosit. Proses tersebut dapat dicegah dengan pemberian antioksidan. Daun kelor termasuk dalam antioksidan alami yang memiliki sifat neurofektif melalui mekanisme antioksidatif. Mengetahui pengaruh pemberian ekstrak daun kelor (Moringa oleifera) Terhadap kadar hemoglobin pada tikus putih (Rattus norvegicus) galur Wistar jantan. Jenis penelitian eksperimental murni (true-experiment) menggunakan pre and post with control group design. Sampel adalah tikus putih (Rattus norvegicus) galur Wistar jantan berusia 1-4 minggu dengan  berat  100-150  gram sejumlah 28 ekor. Sampel dibagi empat kelompok meliputi Kelompok murni (KM) kelompok yang tidak diberikan ekstrak daun kelor, Kelompok Perlakuan 1 (KP1) kelompok yang diberi ekstrak daun kelor dosis 150 mg/kgBB, Kelompok Perlakuan 2 (KP2) kelompok yang diberi ekstrak daun kelor dosis 450 mg/kgBB,dan Kelompok Positif (KP) kelompok yang diberikan suplemen vitamin dosis 5,4 ml/kgBB. Uji Paired T-test menunjukkan tidak ada perbedaan yang bermakna (p>0,05) pada Kelompok Murni (KM) p=0,155, Kelompok Perlakuan 1(KP1) p=0,329, Kelompok Perlakuan 2(KP2) p=0,014 dan Kelompok Positif (KP) p=0,012. Uji Kruskal-Wallis didapatkan p=0,027 (p>0,05)berarti tidak terdapat perbedaan bermakna antar kelompok, uji Post Hoc dengan menggunakan Mann-whitney menunjukkan adanya perbedaan bermakna secara statistik(p<0,05) pada Kelompok Perlakuan 2 (KP2) dengan nilai p=0,002, dan Kelompok Positif (KP) dengan nilai p=0,002. Terdapat pengaruh pemberian ekstrak daun kelor (Moringa oleifera) terhadap kadar hemoglobin tikus putih (Rattus norvegicus) Galur wistar jantan pada  kelompok perlakuan 2 (KP2) dosis 450 mg/kgBB dan Kelompok Positif (KP) dosis 5,4 ml/kgBB.

scholarly journals Potensi Ekstrak Etanol Daun Afrika (Gymnanthemum amygdalinum (Delile) Sch. Bip, Ex walp) Terhadap Penurunan Kadar Glukosa Darah dan Histopatologi Pankreas Tikus Putih Jantan (Rattus norvegicus) yang Diinduksi Streptocotocin dan Pakan Tinggi Lemak

2020 ◽  
Vol 4 ◽  
Author(s):  
Joni Tandi ◽  
Ni Made Irma Mariani ◽  
Ni Putu Setiawati
Keyword(s):  
Rattus Norvegicus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
One Way Anova

Daun afrika (Gymnanthemum amygdalinum (Delile) Sch.Bip.Ex Walp) mengandung alkaloid, flavonoid, saponin, steroid dan tanin. Telah dilakukan penelitian dari ekstrak etanol daun afrika (EEDA) terhadap gambaran histopatologi pankreas dan glukosa darah tikus  putih jantan (Rattus norvegicus) hiperkolesterolemia-diabetes. Tikus hiperkolesterolemia-diabetes diperoleh dengan cara diinduksi pakan tinggi lemak dan streptozotocin. Penelitian bertujuan untuk membuktikan efek EEDA dalam meregenerasi sel β pankreas dan kadar glukosa darah pada tikus hiperkolesterolemia-diabetes, serta mengetahui dosis efektifnya. Jenis penelitian ini adalah true-experimental laboratorium dengan pre and post test with randomized control group design. Subjek penelitian yaitu 35 ekor tikus putih hiperkolesterolemia-diabetes dibagi menjadi 7 kelompok, tiap kelompok terdiri dari 5 ekor. Kelompok I (kontrol normal) dan II (kontrol negatif) diberikan Na-CMC 0,5%, III (kontrol positif 1 ) diberikan simvastatin, IV (kontrol positif 2 ) diberikan metformin, kelompok IV, V, dan VI diberikan EEDA dosis 50; 100; 150 mg/kgBB. Data kadar glukosa darah dan kolesterol total diuji secara statistik (one way ANOVA) dan dilanjutkan uji non parametik (Kruskal-Wallis) pada taraf kepercayaan 95%, jika terdapat perbedaan yang signifikan maka dilakukan uji (Man Whitney) untuk menentukan perbedaan yang berarti dari setiap kelompok. Hasil penelitian menunjukan pemberian EEDA mampu meregenerasi sel β pankreas dan glukosa darah tikus hiperkolesterolemia-diabetes. EEDA dosis 150 mg/kgBB efektif meregenerasi sel β pankreas dan EEDA dosis 150 mg/kg BB efektif terhadap kadar glukosa darah.

scholarly journals Effect of per oral sipermetrin exposure on serum 17 estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

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