scholarly journals MONITORING OF THE SIMMENTAL BREED IN UKRAINE

2017 ◽  
Vol 53 ◽  
pp. 179-184
Author(s):  
A. Ye. Pochukalin ◽  
O. V. Rizun ◽  
S. V. Priyma
Keyword(s):  
High Performance ◽  
Total Population ◽  
Comprehensive Evaluation ◽  
Live Weight ◽  
Animal Husbandry ◽  
The Body ◽  
Average Intensity ◽  
Breeding Lines ◽  
Breeding Stock ◽  
Domestic Breeding

Entry. The genesis of the Simmental breed of milk and meat direction of productivity in Ukraine lasted from imports of foreign (Swiss, Austrian) cattle and the creating of a large array of domestic breeding with a large number of genealogical groups (about 80 lines), to the conversion of the best to speeding up in the domestic Ukrainian Red-and-White Dairy breed dairy productivity. A long process of selection (about one and a half century) has created essentially a new combined Simmental productivity. The array that was created, and that since the mid 90's of the 15 regions and under 39,2 % of the total population of all breeds were characterized by a harmonious structure of the body, strong and dense constitution by well developed muscles. The average live weight of cows of the best breeding plants was 603 kg according to level of milk production of over 5000 kg. The goal of the research was to analyze the breeding stock for the breeding and productive qualities, which ensures systematic monitoring of status and trends of breed processes in population of Simmental breed in Ukraine. Materials and methods of research. Investigations were carried out on breeding stock Simmental 19-herds of 12 regions of Ukraine as of 1 January 2016. For a comprehensive evaluation used data from the population, the productivity of dairy cows (305 days of the last completed lactation), exterior, reproduction, rearing, presence and condition of cows with high performance  and genealogical structure. The results of the research. The driving factor of progress of region breeds is the number of breeding animals in the active population. According to the State registry of subjects of breeding business in animal husbandry the share of Simmental breed in Ukraine on January 1, 2016 the number is 3.1 %, including cows 3.43 percent. So, according to our data selection work with the Simmental breed in Ukraine is carried out in two breeding farms, and 18 breeding reproducers, and their breeding stock has 7840 goals. The average age 4300 Simmental cows in the calving is 3,6 ± 0,30. The distribution of the calving showed a tendency, according to which there is a gradual decrease in the proportion of cows starting with the fresh cows to the fifth calving by 9% and rapid to 10 and older (19.6 %). In breeding nucleus was allocated 1,403 cows, representing 32.6% of the number of the females. It was examined udder shape 900 fresh cows, of which 60% are similar to bath and 40% similar to a cup of milk at an average intensity of 1,90 ± 0,027 kg / min. The intensity of milk output for 45% of the fresh cows is at the level of 1.8 ... of 2.19 kg/min and 21% of fresh cows – above 2,20 kg/min. The type of body structure cows 3913 - 40% were rated "excellent" and only 1% "satisfactory" in 1059 fresh cows corresponding values are 38 and 1%. The genealogical structure of the Simmental breed in Ukraine is represented by two breeds, actually Simmental and Holstein red . Holstein red has 8 lines, among which the most numerous are the Starbuck 352790  (423 goal.) and Chifa 1427381 (323 goal.) for pairing was involved 18 of the bulls in 11 herds with a total population of 1046 of goals, including the 406 cows. Among the lines Simmental only 9.6% of broodstock take seven domestic breeding lines, which are concentrated mainly in the farms of Vinnytsia, Khmelnytsky and Kharkiv regions. Conclusions. Monitoring the condition in the population Simmental in Ukraine and the development of economically useful traits of breeding stock confirmed the general trend of reducing the number. So, if in the early of the 2000s the proportion of Simmental cattle was 5.3%, whereas in 2015 it decreased by 1.8% to 3.5%. Milk yield of cows in the the average is 5373 kg, 3.86% fat content and 3.17% protein in milk by live weight of 579 kg. The average age of disposals is 3.7 cows calving. The main causes of disposal of cows is low productivity and reproductive ability accounting for 54%, the average age of 3.7 calvings. The genealogical structure is represented by lines Simmental (domestic and foreign selection) and Holstein breeds. The share of domestic Simmental breed lines in Ukraine is not more than 10 %.

scholarly journals Hygienic assessment of water quality and its impact on the body of pigs

2020 ◽  
Vol 22 (98) ◽  
pp. 9-15
Author(s):  
N. U. Krempa ◽  
O. V. Kozenko ◽  
N. V. Chornyi
Keyword(s):  
Water Quality ◽  
Single Phase ◽  
Live Weight ◽  
Animal Husbandry ◽  
The Body ◽  
Natural Resistance ◽  
Norm Violation ◽  
Number Of Factors ◽  
Three Phase ◽  
Growth Development

The results of research on the overall impact of drinking water quality, microclimate parameters and adopted in the farms of FE PE “Hlyniany Agro” and LLC “Miasni Resursy” options for growing weaned young (single- and three-phase) on morphological and biochemical parameters of blood, the level of natural resistance and their growth indicates that single-phase cultivation is more favorable for the growth and development of young animals. This is confirmed by the live weight of animals at birth and at the age of 120 days. Thus, in the case of single-phase cultivation of FE PE “Glynyany Agro” the live weight of piglets at birth was 1.11 kg, and in the case of three-phase LLC “Miasni Resursy” – 1.07 kg. When the animals reached 120 days of age, the live weight of piglets was 53.2 kg in FE PE “Glynyany Agro” and 50.2 kg in LLC “Miasni Resursy”. It should be noted that according to most indicators, water quality in both farms is within acceptable limits of sanitary and hygienic requirements, although it needs to be improved. With regard to microclimatic indicators, an overestimated level of ammonia was found in FE PE “Glynyany Agro”, which exceeded the MPC by 6 and 3.33 mg/m3, respectively, in the lines and transition periods, and in LLC “Miasni Resursy” was close to its upper borders. In a farm with single-phase animal husbandry, a slight decrease in erythrocytes in the blood of piglets aged 30 and 60 days was found. Since the animals reached the 120th day of life, an increase in this indicator was noted and the number of erythrocytes in the blood of animals of both farms, within the physiological norm, was 6.47 T/l (FE PE “Hlyniany Agro”) and 7.31 T/l (Miasni Resursy LLC). The concentration of hemoglobin was low in animals of both farms, but the highest rates were in animals of FE PE “Hlyniany Agro” at 30 and 60 days of age, and at 120 days of age this figure (3.28 g/l) was higher already in piglets of Miasni Resursy LLC. The content of total protein in the blood of piglets from both farms was also low, but by the time the animals reached 120 days of age, it was already within the physiological norm. Violation of the albumin-globulin ratio with the vast majority of globulins was also noted. Regarding cellular immunity, it was noted that the content of T-lymphocytes only in animals 120 days of age was normal, exceeding the lower limit of physiological norm by 0.27 % in animals with FE PE “Hlyniany Agro” and 2.09 % in animals with Miasni Resursy LLC. Our research once again confirms that the health, growth, development, and productivity of animals are influenced by a number of factors, both hygienic and technological.

The use distribution properties of Poisson in the production of high-performance feed

2020 ◽  
pp. 7-22
Author(s):  
N. Kostomakhin ◽  
A. Spesivtsev
Keyword(s):  
High Performance ◽  
Live Weight ◽  
Animal Husbandry ◽  
Well Being ◽  
Biologically Active ◽  
Mathematical Concepts ◽  
Feed Production ◽  
Livestock Products ◽  
Livestock Productivity

In a market economics the main reference point for livestock enterprises is the factor of stability and financial well-being. With more than half of all expenses of livestock production is necessary for feed because livestock productivity is mainly dependent on feed, on their efficacy and safety must be first and foremost, the focus in feed production. Only under these environments can the growing productivity of animals, high quality of products and consistent reduction of its primecost be ensured. The influence of the feed base on the development of animal husbandry is shown in the following: properly balanced nutritional feed rations dramatically increase the productivity of animals, increase milk yield, and average daily gains of live weight. The use of biologically and economically effective rations of animals and poultry, first of all, helps to reduce the prime-cost of livestock products, achieve profitability of its production, increase competitiveness in domestic and global markets and ultimately improve the quality of life of Russians. The role of mathematics, including probability theory and mathematical statistics in feed production are indisputable. It makes it possible to build a mathematical model of the object under study, i.e. to describe its most significant features and properties in the language of mathematical concepts and equations. The examples of analytical calculations based on the use of Poisson distribution properties, which allow us to implement a model of high-performance feed with the content of biologically active substances of components in the specified diets of animals and poultry have been provided in the article. The results of the calculations have been summarized in the table to which this brief explanation.

The evaluation of productive traits of pigs with application methods of population selection and molecular genetic markers

2020 ◽  
pp. 36-49
Author(s):  
A. Balnikov ◽  
N. Kostomakhin ◽  
E. Gridyushko ◽  
I. Gridyushko ◽  
V. Ruzuvanova ◽  
...  
Keyword(s):  
Comprehensive Evaluation ◽  
Molecular Genetic ◽  
Average Daily Gain ◽  
Live Weight ◽  
Reproductive Traits ◽  
The Body ◽  
Genetic Studies ◽  
Population Selection ◽  
Breeding Herd ◽  
Productive Traits

The comprehensive evaluation of the productive traits of the initial genotypes of pigs in the creation of breeding herds using population selection methods has been carried out. Based on the results of evaluating the growth and development of breeding animals using the methods of index selection and DNA testing for the genes markers RYR1, ESR, IGF-2, MUC4 the genealogical structure of breeding herds has been formed on the basis of new stud lines of Belarusian stud type of pigs of Yorkshire breed. It has been found that sows of new lines had high reproduction rates: prolifi cacy was 11,6 piglets, milk capacity was 59,1 kg, number of piglets at weaning was 11,2 heads, and litter weight at weaning was 103,6 kg. The breeding herd of two new stud lines is represented by boars of the line Drug 6805 and Dobry 2313. The average productivity of boars-line continuers was: the age of reaching a live weight of 100 kg to 160 days, the average daily gain from birth to reaching the live weight of 100 kg was 636 g. At the age of 12 months, the live weight and body length of replacement boars were 268 kg and 175 cm, the thickness of the fat was 8,9 mm, the height of the longest back muscle was 51,5 mm, the content of lean meat in the body was 62,6 %, which corresponds to the class “elite”. Boars have been separated into reproductive traits. Improvement of lines is carried out through the continuators of related groups. Analysis of genetic studies of animals of new stud lines has shown that they have a high frequency of occurrence of desirable alleles by genes: RYR1 (stress-resistant animals) – 1,0; IGF-2 (fattening and meat traits) – 0,783; ESR (reproductive traits) – 0,608; MUC4 – (resistance to escherichiosis) – 0,786.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

CHARACTERISTICS OF PRODUCTIVE QUALITIES OF FAMILIES OF RED WHITE BELTED PIGS IN SE «RF ZORYANE» BREEDING FARM

2016 ◽  
Vol 52 ◽  
pp. 58-63
Author(s):  
L. V. Onishchenko ◽  
M. I. Danilchuk
Keyword(s):  
High Performance ◽  
Average Daily Gain ◽  
Live Weight ◽  
Litter Weight ◽  
Complex Evaluation ◽  
Appraisal Index ◽  
Daily Gain ◽  
Compactness Index

The most perspective genotype in our region is Red White-belted breed, which has high performance both under pure breeding and under crossing with other breeds. The results of complex evaluation of Red White-belted swine of the herd bred in SE "RF Zoryane" breeding farm are presented. It was revealed that animals of the farm comply with elite class and the first class. The main breeding boars used at the farm belong to such genealogical lines: Deviz, Division, Dantist, Debut, and Dobryak. In the structure of the herd, the most numerous was Dobryak line, which part was 58.8%, Division, Debut, Devis lines – 11.8% per line, Dantist line – 5.8%. The herd sows belong to 7 families: Drabowka, Dekada, Dyktsiya, Doina, Dylema, Delta, Dogma. Average prolificacy of the sows was 10.0 piglets. Litter weight at 60 days’ age was 181.0 kg, and live weight of piglet – 19.0 kg. The performance of sows as prolificacy increased by 0.6 head on average. The complex appraisal index for the herd was 104.1 points. The average daily gain of the replacement pigs was 570 g. The highest daily gain was from five to six months – 611.2 g, and the figure of compactness index characterized a certain elongation of animals, increasing meat traits. Further work with Red White-belted breed aims at preserving and expanding the breeding base and genealogical structure, as well as increasing the reproductive, fattening and meat traits.

Local mineral feed additives in the rations of steers of Hereford breed

2020 ◽  
pp. 28-39
Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya
Keyword(s):  
Economic Effect ◽  
Live Weight ◽  
Negative Influence ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Mineral Salts ◽  
Meat Productivity ◽  
Experimental Group ◽  
Mineral Feed Additives

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.

The effectiveness of use of enterosorbent Shelltic Es when rearing of young pigs

2020 ◽  
pp. 3-11
Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Internal Organs ◽  
Feed Digestibility ◽  
Young Pigs ◽  
Carcinogenic Effects ◽  
Experimental Group

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.

Indicators of metabolism and biochemical status of blood in horses of Yakut breed in winter and spring seasons

2020 ◽  
pp. 52-65
Author(s):  
U. Khompodoeva ◽  
R. Ivanov
Keyword(s):  
Classical Method ◽  
Live Weight ◽  
Weather Conditions ◽  
The Body ◽  
Metabolic Energy ◽  
Temperature Changes ◽  
Experimental Animals ◽  
Spring Period ◽  
Biochemical Status ◽  
Adaptive Mechanisms

The results of researches on the peculiarities of metabolism in the body of horses of Yakut breed in the winter and spring periods under the environments of Central Yakutia have been presented in the article. Physiological experiments on the digestibility of the main nutrients of the feed have been carried out in the winter (February) and spring (April) periods under the environments of the physiological yard in the Yakutsk Scientific and Research Institute of Agriculture named after M. G. Safronov with using the classical method. Five mares and five geldings of Yakut breed have been selected for researches. The average live weight of mares was 378–410 kg, geldings 394–403 kg. The experimental animals had average fatness. It has been found a higher intake of energy and the main nutrients of hay as a mono feed in winter compared to the spring period. At the same time pregnant mares in winter have been consumed 103,8±3,12 MJ of metabolic energy per head/day, which was 27,3 % significantly higher than in the spring period – 75,4±2,18 MJ (P ≥ 0,999). Geldings have been consumed in the experiment 67,70±2,85 MJ, which was by 43,7 % higher than in the spring period – 38,05 ± 4,40 MJ of metabolic energy per head/day (P ≥ 0,999). Experimental animals have been housed in stalls; there was no energy expenditure for movement, so the amount of energy consumed in winter indicates the production of heat in the animal’s body. Pregnant mares per 100 kg of live weight have consumed 31,5±4,11 MJ of metabolic energy or 33,3 % more than in the spring period – 21,0±1,98 MJ; geldings have consumed 27,69±1,12, which was 41,78 % significantly higher than in the spring – 16,12 ± 0,75 MJ (P ≥ 0,999). The high intake of metabolic energy into the body of Yakut horses in winter is explained by the manifestation of adaptive mechanisms that affect the effective metabolism for survival, adapted to frequent changes in weather conditions and sharp temperature changes. The established differences in the biochemical parameters of blood serum of horses of Yakut breed are associated with changes in the consumption and digestibility of basic nutrients and energy, depending on the ambient temperature.

USING THE KAZAKH WHITE-HEADED BREED TO INCREASE BEEF IN THE FLAT PROVINCE OF DAGESTAN

2020 ◽  
Author(s):  
М.М. САДЫКОВ ◽  
М.П. АЛИХАНОВ ◽  
А.Г. СИМОНОВ ◽  
Г.А. СИМОНОВ
Keyword(s):  
Growth And Development ◽  
Live Weight ◽  
Control Group ◽  
Beef Production ◽  
Daily Growth ◽  
Carcass Yield ◽  
Breeding Stock ◽  
Fattening Period ◽  
The Cross ◽  
Experimental Group

Изучены рост и развитие бычков — помесей красной степной с казахской белоголовой породой в равнинной провинции Дагестана. Установлено, что помесный молодняк, по сравнению с чистопородным скотом красной степной породы, имеет более высокую живую массу при рождении на 1,8 кг, или 7,3%. При отъеме в 8-месячном возрасте помеси имели живую массу 210 кг против 187,6 кг у красного степного молодняка, что на 22,4 кг, или 11,9% выше. В 12-, 15- и 18-месячном возрасте живая масса помесных бычков равнялась 291,8 кг, 359,7 и 436,6 кг, соответственно, или на 28 кг, 36 и 45,8 кг больше по сравнению с красным степным скотом. Среднесуточные приросты помесных бычков за период выращивания и откорма составили 760 г, что выше на 12,1% по сравнению с чистопородным красным степным молодняком. После заключительного откорма и убоя помесные бычки дали тяжеловесные туши с хорошим поливом. По массе парной туши они превосходили чистопородных аналогов на 31,3 кг, или 15,6% с преимуществом выхода туши на 2,4%. Убойный выход у бычков опытной группы составил 58,7%, контрольной — 55,8%. От помесных бычков были получены тяжеловесные шкуры массой 27,1 кг, от чистопородных — 21,6 кг, преимущество по этому показателю имели помесные животные 5,5 кг, или 25,4%. Для увеличения производства говядины в равнинной провинции Дагестана рекомендуем скрещивание коров красной степной породы с быками казахской белоголовой породы. The growth and development of bulls of red steppe crossbreeds with the Kazakh white-headed breed in the flat province of Dagestan were studied. It was found that young crossbreed animals in comparison with purebred red steppe cattle have a higher live weight at birth by 1,8 kg or 7,3%. When weaning at 8 months of age, cross-bred bulls had a live weight of 210 kg against 187,6 kg in red steppe young, which is 22,4 kg, or 11,9% higher. At 12, 15 and 18 months of age, the live weight of crossbred bulls was 291,8 kg, 359,7 and 436,6 kg, respectively, or 28 kg, 36 and 45,8 kg more than the red steppe cattle. The average daily growth of cross-bred bulls during the growing and fattening period was 760 g compared to 678 g, that is, they were 12,1% higher compared to purebred red steppe young. After the final fattening and slaughter, the cross-bred bulls gave heavy carcasses with good watering. By weight of the paired carcass, they were superior to purebred counterparts by 31,3 kg or 15,6% with an advantage of 2,4% carcass yield. The slaughter yield of bulls in the experimental group was 58,7%, 55,8% in the control group, respectively, with an advantage in favor of crossbreeds of 2,9%. Heavy skins weighing 27,1 kg were obtained from crossbreed bulls, 21,6 kg from purebred ones, and crossbreed animals of 5,5 kg or 25,4% had an advantage in this indicator. Studies have shown that in order to increase beef production in the lowland province of Dagestan, it is necessary to use crossbreeding of the breeding stock of the red steppe breed with bulls of the Kazakh white-headed breed.

Content and composition of phospholipids, fatty acids and sterols in commercial natural phospholipid excipients

2020 ◽  
Vol 16 ◽  
Author(s):  
Luxia Zheng ◽  
Xiong Shen ◽  
Yingchun Wang ◽  
Jian Liang ◽  
Mingming Xu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cluster Analysis ◽  
Fatty Acid ◽  
High Performance ◽  
Comprehensive Evaluation ◽  
Phospholipid Fatty Acid ◽  
Egg Yolk ◽  
Systematic Research ◽  
Manufacturing Enterprises ◽  
The Many

Background: Phospholipids are widely used in food and pharmaceutical industry as functional excipients. In spite of the many analytical methods reported, there are very limited reports concerning systematic research and comparison of phospholipid excipients. Objective: To present a comprehensive evaluation of commercial natural phospholipid excipients (CNPEs). Methods: Seventeen batches of CNPEs from five manufacturing enterprises, isolated either from soybean or egg yolk, were investigated. The content and composition of phospholipids, fatty acids and sterols as a whole were considered as the evaluative index of CNPEs. Eight kinds of phospholipids were determined by supercritical fluid chromatography (SFC), twenty-one kinds of fatty acids were determined by gas chromatography (GC) after boron trifluoride-methanol derivatization, and nine kinds of sterols were determined by high performance liquid chromatography (HPLC) after separation and derivatization of the unsaponifiable matter. Cluster analysis was employed for classification and identification of the CNPEs. Results: The results showed that each kind of CNPEs had its characteristic content and composition of phospholipids, fatty acids and sterols. Seventeen batches of samples were divided into eight groups in cluster analysis. CNPEs of the same type from different source (soybean or egg yolk) or enterprises presented different content and composition of phospholipids, fatty acids and sterols. Conclusion: Each type of CNPEs had its characteristic content and composition of phospholipid, fatty acid and sterol. The compositions of phospholipid, fatty acid and sterol as a whole can be applied as an indicator of the quality and characteristics for CNPEs.

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