scholarly journals Intermixing of Commercial Pure Breed Chickens with Indigenous (Sakini) Breed of Nepal

2021 ◽  
Vol 7 ◽  
pp. 92-96
Author(s):  
Neena Amatya Gorkhali ◽  
Chhiring Sherpa ◽  
Mana Raj Kolachhapati ◽  
Bhoj Raj Pokharel ◽  
Nirajan Bhattarai ◽  
...  

The intermixing of pure breeds of commercial chickens with Nepalese indigenous flock of chickens has not been explored yet genetically. The study aims to investigate the status of interbreeding of Nepalese indigenous chickens with exotic breeds. Thirty three samples of indigenous chicken, Sakini, from three different ecological regions, Terai, mid-hill and high hill, were taken for the study. The 522 bp hyper-variable region of D loop mtDNA of each sampled population was PCR amplified and sequenced. The Neighbor joining tree revealed that most of Nepalese Sakini and commercial chickens such as New Hampshire, Rhode Island Red, White Leghorn, White Plymouth Rock and Barred Plymouth Rock lied in same clade. Our study provides first direct evidence that the Sakini was already bred with commercial breeds for the sake of enhancing production performance and also alarms the government to introduce proper strategies and policies to avoid such genetic erosion.

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 824
Author(s):  
Sunghyun Cho ◽  
Prabuddha Manjula ◽  
Minjun Kim ◽  
Eunjin Cho ◽  
Dooho Lee ◽  
...  

Korean native chickens (KNCs) comprise an indigenous chicken breed of South Korea that was restored through a government project in the 1990s. The KNC population has not been developed well and has mostly been used to maintain purebred populations in the government research institution. We investigated the genetic features of the KNC population in a selection signal study for the efficient improvement of this breed. We used 600K single nucleotide polymorphism data sampled from 191 KNCs (NG, 38; NL, 29; NR, 52; NW, 39; and NY, 33) and 54 commercial chickens (Hy-line Brown, 10; Lohmann Brown, 10; Arbor Acres, 10; Cobb, 12; and Ross, 12). Haplotype phasing was performed using EAGLE software as the initial step for the primary data analysis. Pre-processed data were analyzed to detect selection signals using the ‘rehh’ package in R software. A few common signatures of selection were identified in KNCs. Most quantitative trait locus regions identified as candidate regions were associated with traits related to reproductive organs, eggshell characteristics, immunity, and organ development. Block patterns with high linkage disequilibrium values were observed for LPP, IGF11, LMNB2, ERBB4, GABRB2, NTM, APOO, PLOA1, CNTN1, NTSR1, DEF3, CELF1, and MEF2D genes, among regions with confirmed selection signals. NL and NW lines contained a considerable number of selective sweep regions related to broilers and layers, respectively. We recommend focusing on improving the egg and meat traits of KNC NL and NW lines, respectively, while improving multiple traits for the other lines.


2019 ◽  
Author(s):  
Grace M Kennedy ◽  
Philip M Panyako ◽  
Philip A Oyier ◽  
Emmanuel K Ndiema ◽  
Jacqueline K Lichoti ◽  
...  

Background: Climate change resulting in heat stress, is one of the most challenging environmental conditions affecting poultry. Studying thermotolerance is crucial in the prevention of heat stress in chickens because it may lead to the identification of genetic lines that can withstand adverse effects of heat stress. This study aimed at investigating polymorphisms in heat shock protein 70 (HSP70) gene in indigenous chicken ecotypes. We also analyzed the mitochondrial DNA (mtDNA) D-loop of indigenous chickens to assess their origins and genetic diversity. Methods: We collected samples From Turkana basin, Mt. Elgon catchment, Lake Victoria basin and Lamu chicken ecotypes in Kenya. Genomic DNA was extracted from 280 chicken samples. The first 360 bp region of HSP70 and the first 760 bp region of the mtDNA were then amplified via PCR. These were later sequenced using Sanger ABI 3730 method. Results: We reveal through a detailed analysis of the HSP70 gene fragment in 20 indigenous chickens the presence of three HSP70 haplotypes (GC, AC, and AG) and 28 mtDNA haplotypes. Phylogenetic analysis of HSP70 revealed the presence of the ancestral haplotype GC which dominated in Turkana basin ecotype. The 28 mtDNA haplotypes clustered in haplogroups A, B, C, D, E and I. Haplogroup E which has never been reported in commercial chickens dominated in Turkana basin ecotype indicating no admixture with commercial chickens. mtDNA haplogroups were shown to have originated from various parts of South and Southeast Asia. Lack of population structure in indigenous chicken ecotypes could be an indication of genetic admixture. The mtDNA nucleotide and haplotype diversity indices were low for Turkana basin ecotype and high for Lamu ecotype. High HSP70 nucleotide diversity indices were recorded in Turkana basin ecotype, while low values were recorded in Lamu ecotype. Most of the mtDNA genetic variations occurred within individuals for the three hierarchical categories considered while most variations in HSP70 gene occurred within populations. Conclusions: This is the first study to analyze the HSP70 polymorphisms in indigenous chickens in Africa and results obtained should pave the way for further in-depth studies on heat stress.


2019 ◽  
Author(s):  
Grace M Kennedy ◽  
Philip M Panyako ◽  
Philip A Oyier ◽  
Emmanuel K Ndiema ◽  
Jacqueline K Lichoti ◽  
...  

Background: Climate change resulting in heat stress, is one of the most challenging environmental conditions affecting poultry. Studying thermotolerance is crucial in the prevention of heat stress in chickens because it may lead to the identification of genetic lines that can withstand adverse effects of heat stress. This study aimed at investigating polymorphisms in heat shock protein 70 (HSP70) gene in indigenous chicken ecotypes. We also analyzed the mitochondrial DNA (mtDNA) D-loop of indigenous chickens to assess their origins and genetic diversity. Methods: We collected samples From Turkana basin, Mt. Elgon catchment, Lake Victoria basin and Lamu chicken ecotypes in Kenya. Genomic DNA was extracted from 280 chicken samples. The first 360 bp region of HSP70 and the first 760 bp region of the mtDNA were then amplified via PCR. These were later sequenced using Sanger ABI 3730 method. Results: We reveal through a detailed analysis of the HSP70 gene fragment in 20 indigenous chickens the presence of three HSP70 haplotypes (GC, AC, and AG) and 28 mtDNA haplotypes. Phylogenetic analysis of HSP70 revealed the presence of the ancestral haplotype GC which dominated in Turkana basin ecotype. The 28 mtDNA haplotypes clustered in haplogroups A, B, C, D, E and I. Haplogroup E which has never been reported in commercial chickens dominated in Turkana basin ecotype indicating no admixture with commercial chickens. mtDNA haplogroups were shown to have originated from various parts of South and Southeast Asia. Lack of population structure in indigenous chicken ecotypes could be an indication of genetic admixture. The mtDNA nucleotide and haplotype diversity indices were low for Turkana basin ecotype and high for Lamu ecotype. High HSP70 nucleotide diversity indices were recorded in Turkana basin ecotype, while low values were recorded in Lamu ecotype. Most of the mtDNA genetic variations occurred within individuals for the three hierarchical categories considered while most variations in HSP70 gene occurred within populations. Conclusions: This is the first study to analyze the HSP70 polymorphisms in indigenous chickens in Africa and results obtained should pave the way for further in-depth studies on heat stress.


2018 ◽  
Vol 12 (2) ◽  
pp. 313-328
Author(s):  
Fathul Aminudin Aziz

Fines are sanctions or punishments that are applied in the form of the obligation to pay a sum of money imposed on the denial of a number of agreements previously agreed upon. There is debate over the status of fines in Islamic law. Some argue that fines may not be used, and some argue that they may be used. In the context of fines for delays in payment of taxes, in fiqh law it can be analogous to ta'zir bi al-tamlīk (punishment for ownership). This can be justified if the tax obligations have met the requirements. Whereas according to Islamic teachings, fines can be categorized as acts in order to obey government orders as taught in the hadith, and in order to contribute to the realization of mutual benefit in the life of the state. As for the amount of the fine, the government cannot arbitrarily determine fines that are too large to burden the people. Penalties are applied as a message of reprimand and as a means to cover the lack of the state budget.


2020 ◽  
Vol 7 (1) ◽  
pp. 126 ◽  
Author(s):  
Fradhana Putra Disantara

This study aims to analyze the relevance of the �health emergency� status to the existing legal theory and condition as well as to identify the validity of the Circular Letter of the Rector of State Universities. To this end, this study applied the statute and conceptual approach. The study was conducted by inventorying primary and secondary legal materials to obtain a proper and critical review of the legal issues under study. The results showed that the determination of the �health emergency� status by the government was inappropriate due to the uncertainty of the regulations issued by the government to determine the current condition. Thus, the status of the COVID-19 pandemic is a �legal emergency� status. Further, the Rector�s policy through the Circular Letter is valid judicially, sociologically, and philosophically. The determination of the �legal emergency� status can be done by issuing a Perppu without a �state of emergency� from the President. Finally, it is suggested to firstly get an approval from the Ministry of Education and Culture regarding the issuance of the Rector�s Circular Letter. Besides, further study is needed as this study was conducted during the COVID-19 pandemic.�Keabsahan Surat Edaran Rektor Perguruan Tinggi dalam Pandemi Covid-19Tujuan dari penelitian ini adalah untuk menganalisa relevansi status �darurat kesehatan� dengan teori hukum dan kondisi yang ada dan keabsahan atas Surat Edaran Rektor Perguruan Tinggi Negeri. Metode yang digunakan dalam penelitian ini adalah statute approach dan conseptual approach. Penelitian dilakukan dengan menginventarisasi bahan hukum primer dan sekunder, guna mendapatkan kajian yang seyogianya dan telaah kritis terkait isu hukum. Hasil penelitian menyatakan penetapan status darurat kesehatan oleh pemerintah kurang tepat, dikarenakan tidak menentu-nya peraturan yang dikeluarkan oleh pemerintah untuk menetapkan kondisi saat ini. Sehingga, status pandemi COVID-19 merupakan status darurat hukum. Kebijakan rektor melalui Surat Edaran adalah absah secara aspek yuridis, sosiologis, dan filosofis. Penetapan darurat hukum cukup dilakukan dengan menerbitkan Perppu tanpa pernyataan darurat dari Presiden. Saran peneliti adalah di perlukan persetujuan pada Kementerian Pendidikan dan Kebudayaan terkait terbitnya Surat Edaran Rektor, dan dibutuhkan penelitian lebih lanjut dikarenakan penelitian ini dilakukan pada masa COVID-19 yang bersifat temporal.�


Author(s):  
Markus T Lasut ◽  
Adianse Tarigan

A study on water quality status of three riverine systems, S. Bailang (SB), S. Maasing (SM), and S. Tondano (ST), in coastal city of Manado, North Sulawesi Province, has been conducted to measure several water quality parameters, to analyse source and quality of wastewater discharge, and to assess the status of the rivers related to the water quality. Measurement of the parameters was conducted using three indicators, i.e. organic (BOD5) and in-organic (N-NO3 and P-PO4), and pathogenic microorganism (Escherichia coli [EC] and total coliform [TC]). The result showed that the level of water quality varied between the rivers. The average level of water quality (based on the observed parameters) in SB, respectively, was 0.317 mg/l, 0.093 mg/l, 2 mg/l, >2420 MPN, and  >2420 MPN; in SM, respectively, was 0.029 mg/l, 1.859 mg/l, 17.7 mg/l, >2420 MPN, and >2420 MPN; and in ST, respectively, was 0.299 mg/l, 0.252 mg/l, 3.5 mg/l, >2420 MPN, and >2420 MPN. The level of water quality between the rivers was not significantly different (p>0.05), except based on the parameter of N-NO3 which was significantly different (p<0.01). The status of the observed rivers varied based on the classes of their water utilities (according to the Government Regulation of Indonesia, No. 82, 2001); mostly was "unsuitable". Kajian tentang status kualitas air di 3 perairan sungai di kota pesisir Manado, S. Bailang (SB), S. Maasing (SM), dan S. Tondano (ST), Provinsi Sulawesi Utara, telah dilakukan yang bertujuan untuk mengukur beberapa parameter kualitas air, menganalisis sumber dan kualitas buangan limbah domestik, dan menilai status ketiga perairan sungai tersebut. Tiga indikator digunakan, yaitu: bahan organik (BOD5), bahan anorganik (N-NO3 dan P-PO4), dan mikroorganisme patogenik (Escherichia coli [EC] dan coliform total [TC]). Hasil kajian menunjukkan bahwa tingkat kualitas air perairan tersebut berbeda-beda. Konsentrasi rerata parameter kualitas air  (BOD5, N-NO3, P-PO4, EC, dan TC) di SB, berturut-turut, sebesar 0.317 mg/l, 0.093 mg/l, 2 mg/l, >2420 MPN, dan >2420 MPN; di SM, berturut-turut, sebesar 0.029 mg/l, 1.859 mg/l, 17.7 mg/l, >2420 MPN, dan >2420 MPN; dan di ST, berturut-turut, sebesar 0.299 mg/l, 0.252 mg/l, 3.5 mg/l, >2420 MPN, dan >2420 MPN. Konsentrasi kualitas air ketiga sungai tersebut tidak berbeda secara signifikan (p>0.05), kecuali parameter N-NO3 (p<0.01). Secara umum, kondisi kualitas air ketiga sungai tersebut, menurut Peraturan Pemerintah No. 82, 2001) berada dalam status “tidak cocok” untuk peruntukannya.


Author(s):  
Shalakha Rao ◽  
Shivani Kushwaha

The connection between poverty and women's lack of power over resources and decision-making has now caught the attention of policymakers in government and mainstream development all over the world. Women empowerment issues perceived nationally or locally are being addressed by both state and non-state agencies. Beside the government intervention, NGOs are implementing various types of Women Empowerment Programmes including IG Programmes. Women Empowerment Programmes in India include livelihood support Programme, rehabilitation and job placement for rescued women, safe motherhood Programme and so forth. In spite of involvement of various NGOs in women empowerment through Income Generation and Skill Development Programmes, the status of women is still not satisfactory in India as various official as well as unofficial reports claim and the outcomes against the stated objectives of the NGOs' Women Empowerment Programmes are often questioned. Therefore, the present study is focused in assessing the impact of IG Programmes run by non-government organizations in empowering women. The researcher hypothesizes that IG Programme with its components viz., skill training, resource inputs of loan and equipment help to increase income to the women through independent business or work in the related field; the increased income lessens their dependence on family heads and enables to spend for personal expenses; gives them certain freedoms as individuals; enables them to contribute to family affairs financially, which creates an environment in the family in favor or the women to accept her views and participation in family matters like education, marriage, purchase etc.


2020 ◽  
Author(s):  
Jeya Sutha M

UNSTRUCTURED COVID-19, the disease caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a highly contagious disease. On January 30, 2020 the World Health Organization declared the outbreak as a Public Health Emergency of International Concern. As of July 25, 2020; 15,947,292 laboratory-confirmed and 642,814 deaths have been reported globally. India has reported 1,338,928 confirmed cases and 31,412 deaths till date. This paper presents different aspects of COVID-19, visualization of the spread of infection and presents the ARIMA model for forecasting the status of COVID-19 death cases in the next 50 days in order to take necessary precaution by the Government to save the people.


2021 ◽  
Vol 13 (8) ◽  
pp. 4339
Author(s):  
Aditi Khodke ◽  
Atsushi Watabe ◽  
Nigel Mehdi

In the face of pressing environmental challenges, governments must pledge to achieve sustainability transitions within an accelerated timeline, faster than leaving these transitions to the market mechanisms alone. This had led to an emergent approach within the sustainability transition research (STR): Accelerated policy-driven sustainability transitions (APDST). Literature on APDST asserts its significance in addressing pressing environmental and development challenges as regime actors like policymakers enact change. It also assumes support from other incumbent regime actors like the industries and businesses. In this study, we identify the reasons for which incumbent industry and business actors might support APDST and whether their support can suffice for implementation. We examine the actor strategies by drawing empirical data from the Indian national government policy of mandatory leapfrog in internal combustion engine (ICE) vehicle emission control norms, known as Bharat Stage 4 to 6. This leapfrogging policy was introduced to speed up the reduction of air pollutants produced by the transport sector. A mixed-methods approach, combining multimodal discourse analysis and netnographic research, was deployed for data collection and analysis. The findings show that unlike the status quo assumption in STR, many incumbent industry and business actors aligned with the direction of the enacted policy due to the political landscape and expected gains. However, the degree of support varied throughout the transition timeline and was influenced by challenges during the transitioning process and the response of the government actors. The case suggests we pay more attention to the actors’ changing capacities and needs and consider internal and external influences in adapting the transition timelines. This study contributes to the ongoing discussion on the implementation of APDST, by examining the dynamism of actor strategies, and provides an overview of sustainability transitions in emerging economies.


2020 ◽  
pp. 194277862097931
Author(s):  
Halley L. Glier ◽  
Temperance Staples ◽  
Megan Martínez ◽  
Anita Fábos ◽  
S.E.D. Mitchell ◽  
...  

This paper draws on observational research conducted in McAllen, Texas, during the summer of 2019, of three major stakeholder groups involved in asylum management: Catholic Charities Humanitarian Respite Center (HRC); federal government agencies; and the McAllen community. Each group holds a unique, pluralistic perspective on migration, informing intra-group relations and exposing uneven power dynamics between them. Our analysis is contextualized by a local voice, a former long-term volunteer at the HRC, who speaks of the evolution of the McAllen border in her lifetime, as well as federal authority over McAllen and the HRC to process asylees. We dissect how this power dynamic produces a highly violent, detention-dominant immigration landscape in the Rio Grande Valley (RGV), antithetical to the practiced intersectional culture of voces locales. We reimagine how the US responds to asylum seekers by offering a community action-based framework, where these pluralistic perspectives are equitably valued. Based on interactions and conversations had with each group, we advocate a paradigm shift reflective of La Frontera’s (The Border’s) intersectional identity. This can be achieved by prioritizing voces locales and building capacity for the humanitarian sector, which is already doing critical work at the southern border. We look to contemporary movements like “Defund the Police” as examples, where divesting from the status-quo system of oppression can nurture reparative justice and empowerment to the RGV. In reimagining a more adaptive, asylum justice-oriented paradigm shift, we also recognize the need to abandon the government-controlled deterrence paradigm, which repeatedly causes tremendous harm.


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