scholarly journals Pollen viability of ‘Besztercei plum' clones depending on the effect of the year

2000 ◽  
Vol 6 (3) ◽  
Author(s):  
A. Horváth ◽  
Zs. Orosz-Kovács ◽  
D. Surányi ◽  
Z. Erdős ◽  
S. Gulyás ◽  
...  

The percentage of dark staining pollen grains was higher in spring of 1996 than in the previous year. Data in 1998 resemble those of 1995, concerning the large amount of medium staining pollen grains in the majority of clones. Some clones produced excellent quality pollen also in the third year. whereas there were significant differences in other clones in various years. The warmer February-March period in 1995 induced an early blooming, and frost affected the orchard not only in winter months, but also immediately before and during blooming. Thus, frost was the possible cause of weaker quality pollen this year. In 1996 warming began a bit late, but it was not broken by drastic falls in temperature, except for the middle of April, when a smaller frost affected the orchard. It is likely that this frost did not influence pollen quality of `Besztercei' and 'Early Besztercei' plum clones significantly. In 1998 warming was continuous and steady, the orchard was not affected by frost immediately before blooming. In March, however, there was frost almost every day, according to daily minimum temperatures.

Genetika ◽  
2017 ◽  
Vol 49 (1) ◽  
pp. 105-115 ◽  
Author(s):  
Dusica Calic ◽  
Ljiljana Radojevic

Pollen quality of horse chestnut, expressed as pollen productivity, viability and germination was studied. Anthers of horse chestnut genotypes had pollen production from 3.66 to 5.06 x 103 pollen grains per anther, depending of genotype. Also, pollen of horse chestnut Ah1-Ah4 genotypes showed different viability (from 56 to 68%), after staining with fluorescein diacetate. Pollen germination of Ah1-Ah4 genotypes varied from 50-66% on basic medium. Inclusion of polyethylene glycol-PEG from 10%, 15% and 20% v/w increased pollen germination. The best results were achieved on medium with the largest PEG concentration. On these medium 76-91% pollen grains were germinated, depending of genotype. The best pollen quality, for all tested parameters, had genotype Ah2. Knowledge about morphology, production, viability, in vitro germination, tube growth as well as pollen: ovule ratio can be of great importance for future pollen biology studies.


Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2222-2227
Author(s):  
Andrea Sotomayor ◽  
Jorge Merino ◽  
William Viera

The germination and viability of pollen are characteristics required for fecundation when individuals of different or the same species are crossed. For this reason, assessing these parameters in selected individuals to be used in breeding programs will increase the chances for the obtainment of new progeny. In this study, pollen from different accessions of the red-purple tree tomato (Solanum betaceum Cav.) was used: local cultivar (Morado Puntón), two commercial varieties (Large Red and Oratia Red) and six segregants [(Solanum unilobum x Solanum betaceum) x Solanum betaceum]. Three types of flowers were taken (A-day of anthesis, B-one day after anthesis, and C-two days after anthesis). The pollen was conserved in two temperatures (4° and 22° C) and four storage times (0, 5, 10, 20 days). The percentage of germination and pollen viability of the selected individuals were evaluated. It was observed that the commercial materials showed higher germination percentages than the segregants in flower A and B at a temperature of 4 ° C at all storage times, except for the segregants GT7P47 and GT7P48 at the same temperature on day 0. In addition, high percentages of viability were obtained both in flowers A and B, at both temperatures and at all storage times. However, the immediate use of pollen after it is collected is recommended because better germination is achieved. This study is helpful to improve breeding procedures in the initial stages of directed crosses.


HortScience ◽  
2020 ◽  
Vol 55 (2) ◽  
pp. 258-260
Author(s):  
Akide Özcan

The objective of this study was to analyze the effects of low storage temperatures on the quality of pollen obtained from the sweet cherry cultivars 0900 Ziraat, Regina, Starks Gold, and Sweet Heart. The pollen was stored at 4 °C, –20 °C, and –80 °C for 12 months, and its viability and germinability were determined at 3-month intervals. The results demonstrated that the initial pollen viability varied between 73.62% and 79.37%, while pollen germinability varied between 41.24% and 53.56%. The percentage of pollen viability declined remarkably from the third to 12th month by almost 3% in 4 °C storage. The pollen viability for the other two storage temperatures (–20 °C and –80 °C) was greater than 50% by the end of the 12th month. It can be concluded that the pollen quality of these cultivars can be preserved sufficiently at temperatures less than –20 °C.


2021 ◽  
pp. 16-21
Author(s):  
T. I. Saltanovish ◽  
L. I. Andronic ◽  
L. P. Antosh ◽  
A. N. Doncila

Relevance. The action of viral phytopathogens changes the quality of the male gametophyte, which ultimately leads to a decrease in plant productivity. So, information on the features of the variability of morphofunctional characteristics of the microgametophyte becomes topical. Taking into account the expression of a part of the plant genome in pollen, the male gametophyte can be used as a reliable system for assessing the response of genotypes to the action of viral pathogens. Presuming that the problems of variability of characteristics of the male gametophyte in conditions of viral pathogenesis have not been enough studied, as well as the main factors determining the variability have not been identified, the aim of the research was to study the effect of viral phytopathogens on the male gametophyte characters in tomato.Material and methods. Hybrids F1 and varieties of tomato were used in the experiments. Plants were grown in a greenhouse. They were inoculated with tobacco mosaic virus (TMV) or tomato aspermia virus (TAV) in the 4-5 leaf phase. To determine the quality of pollen, flowers of control and infected plants were collected; pollen was isolated and sown on a nutrient medium. The preparations were analyzed under microscope. The viability of the pollen and the length of the pollen tubes were assessed, and the ratio of these indicators was calculated.Results. Infection of plants with viruses changes some functional characteristics of the male gametophyte. The responses manifested as stimulation, inhibition, or neutral effect. The main sources of variability in characters of pollen were the genotype and viral agents. In viral pathogenesis, unequal indicators of pollen grains were found in terms of the rate of germination and growth of pollen tubes (PT). Analysis of the heritability coefficients of the ratio of pollen viability and PT length (experiment / control) revealed positive dominance and overdominance in 70% of cases. Genotypes have been identified that combine a high level of viability when infected with different viruses. The prospect of their use in further research is proposed. 


2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen


Author(s):  
Ekaterina Grinberg

In two parts of the article, more than 70 errors and violations of the Biotechnics of artificial breeding of Pacific salmon in salmon hatcheries are collected and summarized, which lead to an immediate or delayed deterioration of the quality of reared fry and a decrease in their survival rate. Deviations from the biological basis of fish farming are shown and possible consequences of such errors or violations are predicted. In the first part of the article (#7,2020) there were General errors (throughout the entire fish-breeding process), as well as violations of Biotechnics during the work with producers, collecting eggs and preparing them for incubation, in the second part-violations of Biotechnics during the incubation of eggs, holding pre-larvae, rearing and release of young. Their correction, prevention or minimization of consequences will significantly improve the quality of sexual products and offspring from producers, increase the linear and weight gain of juveniles during rearing, improve the coefficient of feed payment, reduce the time of rearing, improve the epizootic situation by developing immunity in fry and mobilizing other protective reactions of their body, significantly reduce waste at all stages of the production process, increasing survival by 10–15%. In conclusion, it is noted that the first and most important condition for an efficient salmon hatchery is the creation of optimal environmental conditions at each stage of the production process. The second condition is strict compliance with the Biotechnics of artificial salmon breeding and the complex of veterinary and sanitary, fish-breeding and meliorative and therapeutic and preventive measures. The third is the availability of competent specialists who regularly improve their skills in accredited Universities to implement, control and manage the first two conditions.


2014 ◽  
Vol 4 (2) ◽  
pp. 54-78
Author(s):  
Petr Adamec ◽  
Marián Svoboda

This paper deals with the results of sociological survey focused on identification of the attitudes of elderly people to further education. The research was carried out in September 2010. Experience of elderly people with further education, their readiness (determination) for further education as well as their motivation and barriers in further education were also subjects of this research. Detecting elderly population’s awareness of universities of the third age and finding out their further education preferences were an integral part of the research. Research sample consisted of citizens over 55 years living in the South Moravian region. The survey results are structured by socio-demographic features e.g.: age, sex, educational attainment etc. and provide an interesting insight into the attitudes of the target group to one of the activities that contributes to improvement of their quality of life.


Author(s):  
Howell A. Lloyd

Bodin arrived in Toulouse c.1550, a brief account of the economy, social composition, and governmental institutions of which opens the chapter. There follow comments on its cultural life and identification of its leading citizenry, with remarks on the treatment of alleged religious dissidents by the city itself, and especially on discordant intellectual influences at work in the University, most notably the Law Faculty and the modes of teaching there. The chapter’s second part reviews Bodin’s translation and edition of the Greek poem Cynegetica by Oppian ‘of Cilicia’, assessing the quality of his editorial work, the extent to which allegations of plagiarism levelled against him were valid, and the nature and merits of his translation. The third section recounts contemporary wrangling over educational provision in Toulouse and examines the Oratio in which Bodin argued the case for humanist-style educational provision by means of a reconstituted college there.


2020 ◽  
Vol 8 ◽  
pp. 232470962095777 ◽  
Author(s):  
Maria Elena Romero-Ibarguengoitia ◽  
Consuelo Cantú-Reyna ◽  
Dalia Gutierrez-González ◽  
Héctor Cruz-Camino ◽  
Arnulfo González-Cantú ◽  
...  

The intellectual disability syndrome characterized by seizures and dysmorphic features was initially described in 2017 and was associated with genetic variants in the OTUD6B gene, identified by exome sequencing (ES) in a large cohort. This multisystem disorder primarily affects the central nervous system, the gastrointestinal, and the skeletal systems. In this article, we describe the first Mexican patient diagnosed by ES. The homozygous c.433C>T (p.Arg145*) variant of the OTUD6B gene confirmed this intellectual disability syndrome. In addition to seizures and other more frequently reported manifestations of this condition, this is the third patient with associated hypothyroidism and hypogammaglobulinemia, underscoring the value of screening for these conditions in other patients. The current challenge with this patient is to ensure medical management of his seizures and provide him with a better quality of life. The possibilities of additional therapeutic approaches may increase by understanding the physiopathology of the involved pathways.


Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 4046
Author(s):  
Mateusz Bronis ◽  
Edward Miko ◽  
Lukasz Nowakowski

This article discusses the relationship between the kinematic system used in drilling and the quality of through-holes. The drilling was done on a CTX Alpha 500 universal turning center using a TiAlN-coated 6.0 mm drill bit with internal cooling, mounted in a driven tool holder. The holes were cut in cylindrical 42CrMo4 + QT steel samples measuring 30 mm in diameter and 30 mm in length. Three types of hole-drilling kinematic systems were considered. The first consisted of a fixed workpiece and a tool performing rotary (primary) and linear motions. In the second system, the workpiece rotated (primary motion) while the tool moved linearly. In the third system, the workpiece and the tool rotated in opposite directions; the tool also moved linearly. The analysis was carried out for four output parameters characterizing the hole quality (i.e., cylindricity, straightness, roundness, and diameter errors). The experiment was designed using the Taguchi approach (orthogonal array). ANOVA multi-factor statistical analysis was used to determine the influence of the input parameters (cutting speed, feed per revolution and type of kinematic system) on the geometrical and dimensional errors of the hole. From the analysis, it is evident that the kinematic system had a significant effect on the hole roundness error.


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