EFFECT OF NIFEDIPINE ON APOPTOSIS, NECROSIS AND VIABILITY OF GERMINAL EPITHELIAL CELLS IN THE CONTRALATERAL TESTICLE AND PLASMA TESTOSTERONE LEVELS IN MALE WHITE RATS (RATTUS NORVEGICUS, WISTAR STRAIN) AFTER UNILATERAL TESTICULAR TORSION

Objective: To compare the number of apoptosis, necrosis and viability of germinal epithelial cells in the contralateral testicle (CT) and plasma testosterone (PT) levels in white male rats which administered Nifedipine after unilateral testicular torsion (TT) compared with control group. Material & Methods: This research was an experimental study using male white rats (Rattus Norvegicus, Wistar strain) aged 10-12 weeks and body weight 150-200 gram. A total of 30 rats were then randomly divided into 5 groups (n=6) which were negative control group (KN), positive control group (KP1 and KP2) and Nifedipine-administered group (N1 and N2). Each group performed unilateral left side torsion of testicular of 1080o anticlockwise except the KN group. There was 4-hour ischemic duration in the KP1 and N1 groups while 10 hours in the KP2 and N2 groups. Administration of Nifedipine 30 minutes before detorsion by intraperitoneal injection dosed 100 μg/kgBW. All groups performed right orchidectomy and plasma blood sampling. Measurement of apoptosis, necrosis and viability of germinal epithelial cells in the CT using flowcytometry. Measurement of PT levels using Enzyme-Linked Immunosorbent Assay (ELISA). Results: The number of apoptosis, necrosis and viability of contralateral testicular germinal epithelial cells and PT levels in the KN group compared with KP1 and KP2 groups were significantly different (p<0.05). There was no significant discrepancy in apoptosis (p>0.05) in KP1 group compared with N1 group, as well as in KP2 group compared with N2 groups. The number of necrosis, viability of germinal epithelial cells in the CT and PT level in KP1 group in compared with N1 group, as well as in KP2 group in compared with N2 group were significantly different (p<0.05). Conclusion: Nifedipine administration prior to testicular detorsion can maintain cell viability and decrease the amount of necrotic germinal epithelial cells in the CT and prevent the decrease in PT levels after unilateral TT.

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UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽

10.35799/pha.9.2020.31367 ◽

2020 ◽

Vol 9 (4) ◽

pp. 572

Author(s):

Asriyani Samiun ◽

Edwin De Queljoe ◽

Irma Antasionasti

Keyword(s):

Rattus Norvegicus ◽

Ethanol Extract ◽

Male Rats ◽

Test Material ◽

Control Group ◽

White Rats ◽

Wistar Strain ◽

One Way Anova ◽

Vernonia Cinerea ◽

Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

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THE EFFECTIVENESS OF CORIANDER (Coriandrum sativum L.) EXTRACTS ON REDUCING THE BLOOD SUGAR LEVELS OF ALLOXAN INDUCED WISTAR MALE RATS (Rattus norvegicus L.)

Jurnal Kedokteran RAFLESIA ◽

10.33369/juke.v6i1.10979 ◽

2020 ◽

Vol 6 (1) ◽

pp. 36-44

Author(s):

Kalvin Dersing

Keyword(s):

Blood Sugar ◽

Rattus Norvegicus ◽

Water Extract ◽

Coriandrum Sativum ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Wistar Strain ◽

Sugar Levels

AbstractBackground : Diabetes mellitus is the most common metabolic syndrome in the whole world with an incidence of 1-8%. This disease arises when insufficient insulin is produced or insulin cannot function properly.The ability of coriander juice water extract is expected to reduce blood glucose levels because the action is similar to insulin and can stimulate insulin release. Purpose : Knowing effectiveness coriander extract (Coriandrum sativum L.)to decline blood sugar levels in male white rats (Rattus norvegicus L.) wistar strain induced by alloxan. Method : This is a pure experimental study (true-experiment) using a pre and post test design with control group design using subjects namely white rats (Rattus norvegicus L.) male Wistar strain aged 10-12 weeks with a weight of 150-200 grams. By looking at changes in blood sugar levels during administration of alloxan and administration of coriander extract. Results : There were significant changes in some groups induced alloxan (p <0.05), but the effect of coriander extract was not very significant (p> 0.05). Conclusion : Coriander extract can control blood sugar levels to be stable or normal, but it takes longer and more research. Keywords : coriander extract, blood sugar levels, alloxan

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PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v20i2.28 ◽

2019 ◽

Vol 20 (2) ◽

pp. 70-75

Author(s):

Yuliyanti Yuliyanti ◽

A. Sareh Arjono Tjandra ◽

Herin Setianingsih

Keyword(s):

Rattus Norvegicus ◽

High Fat Diet ◽

White Male ◽

Male Rats ◽

Control Group ◽

Standard Diet ◽

High Fat ◽

Malus Sylvestris ◽

Wistar Strain ◽

Significant Difference

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects. Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties

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The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6416 ◽

2021 ◽

Vol 9 (B) ◽

pp. 670-678

Author(s):

Nurdiana Nurdiana ◽

Pradnyawati Chania ◽

Rifzi Nurvitasari ◽

Azmiatun Nisa ◽

Styan Wahyu Diana ◽

...

Keyword(s):

Rattus Norvegicus ◽

Wistar Rats ◽

Reproductive Development ◽

Normal Diet ◽

Male Rats ◽

Male Rat ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Soy Milk ◽

Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope. RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

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Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

Jurnal Ilmiah Keperawatan STIKES Hang Tuah Surabaya ◽

10.30643/jiksht.v12i1.37 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Idola Perdana Sulistyoning Suharto

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Male Rats ◽

Control Group ◽

P Value ◽

Control Group Design ◽

White Rats ◽

Significant Difference ◽

Phaleria Macrocarpa ◽

Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound

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Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

KnE Life Sciences ◽

10.18502/kls.v3i6.1200 ◽

2017 ◽

Vol 3 (6) ◽

pp. 702 ◽

Cited By ~ 1

Author(s):

Dwi Gayatri Nurcahyawati ◽

Hani Plumeriastuti ◽

Lilik Maslachah

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Oral Therapy ◽

White Male ◽

Male Rats ◽

Male Rat ◽

Glomerular Sclerosis ◽

Control Group ◽

Per Oral ◽

Tuber Extract

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly. Key words: Eleutherine palmifolia, alloxan, kidney, histhopathology

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Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

Oceana Biomedicina Journal ◽

10.30649/obj.v3i2.47 ◽

2020 ◽

Vol 3 (2) ◽

pp. 38

Author(s):

Ayu Mayang A. P. ◽

Liliawanti Liliawanti ◽

Wachjudi Kurnia

Keyword(s):

Rattus Norvegicus ◽

White Male ◽

Brown Seaweed ◽

Negative Control ◽

Laboratory Method ◽

The Body ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Significant Difference

Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (Sargassum duplicatum) which can increase hematocrit levels in the blood of white male wistar strain rat (Rattus norvegicus) suffered from anemia in induction NaNO2. This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K0) without treatment, the positive control (K1) be treated anemia given NaNO2 for 18 days without Sargassum duplicatum extract and treatment group (K2) treated with anemia given NaNO2 for 18 days without Sargassum duplicatum extract at a dose of 2,45gr / kgBB. On the 40th day, a blood sample was taken, then measured the hematocrit level. The results showed that the mean number of blood hematocrit levels in K2 was higher than K1, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p > 0,05 showed that there were no statistically significant differences between groups of experimental animals. From the results of this research, the conclusion is the administration of brown seaweed extract (Sargassum duplicatum) descriptively increased, but not statistically significant. Keywords : Anemia, hematocrit, Sargassum duplicatum, NaNO2

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The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

Berkala Kedokteran ◽

10.20527/jbk.v15i2.7128 ◽

2019 ◽

Vol 15 (2) ◽

pp. 71

Author(s):

I Made Subhawa Harsa

Keyword(s):

Free Radicals ◽

Treatment Group ◽

Cigarette Smoke ◽

Rattus Norvegicus ◽

White Male ◽

Male Rat ◽

Special Treatment ◽

Control Group ◽

Wistar Strain ◽

Group 2

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

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THE EFFECTIVENESS OF 70% METHANOLIC EXTRACT OF AVOCADO LEAF (Persea americana Mill) IN DECREASING BLOOD SUGAR LEVELS IN MALE RATS (Rattus norvegicus) WISTAR STRAIN INDUCED ALLOXAN

Biomedika ◽

10.23917/biomedika.v8i1.3018 ◽

2017 ◽

Vol 8 (1) ◽

Cited By ~ 1

Author(s):

Retno Sintowati ◽

Anindita Putri Handayani ◽

Riandini Aisyah

Keyword(s):

Diabetes Mellitus ◽

Rattus Norvegicus ◽

Methanolic Extract ◽

Persea Americana ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Wistar Strain ◽

Sugar Levels

Tanaman alpukat (Persea americana Mill) merupakan tanaman tradisional yang memiliki kandungan flavonoid dan tannin yang dapat menurunkan kadar gula darah. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak metanol 70% daun alpukat (Persea americana Mill) terhadap penurunan kadar gula darah.Metode penelitian menggunakan eksperimental laboratorik dengan rancangan penelitian pretest – posttest with control group design. Hewan uji yang digunakan 25 ekor tikus jantan galur Wistar, umur 2-3 bulan, dan berat badan 150-200 gram yang dibagi dalam 5 kelompok. Kelompok I: kontrol negatif (aquades), kelompok II: kontrol positif (glibenklamid), kelompok III, IV, V : ekstrak metanol 70% daun alpukat dengan dosis berturut-turut adalah sebagbai berikut: 100 mg/ 200 grBB, 150 mg/ 200 grBB, 200 mg/ 200 grBB. Gula darah diukur dengan alat spektrofotometer. Berdasarkan hasil uji ANOVA, data penurunan kadar gula darah hari ke-7 pemberian ekstrak nilai probabilitas signifikan p = 0, 000 dengan demikian p < 0, 05 maka terdapat perbedaan penurunan kadar gula darah yang bermakna. Berdasarkan uji LSD dari semua kelompok didapatkan nilai p < 0,05 yang berarti terdapat perbedaan penurunan kadar gula darah yang signifikan antara2 kelompok. Kesimpulan: Ekstrak metanol 70% daun alpukat (Persea americana Mill) dosis 200 mg/200; 100 mg/200 and 150 mg/ 200 gram/berat badan dapat menurunkan kadar gula darah pada mencit jantan galur wistarKata Kunci : Ekstrak metanol 70%, Alpukat (Persea americana Mill), gula darah, diabetes mellitus.

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Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v9i1.664 ◽

2020 ◽

Vol 9 (1) ◽

pp. 21

Author(s):

I Made Subhawa Harsa

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Centella Asiatica ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

White Rats ◽

Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica) is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

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