scholarly journals the influence of giving katu’s leaf extract fraction toward prolactin level of breastfeeding mice and neuralgia cells of young mice

2015 ◽  
Vol 5 (2) ◽  
Author(s):  
Nurul Kamariyah

People knew that Sauropus androgynus (L) Merr (katu’s leaf) have good nutrient and can enhance breast milk production. Study about influence of giving katu’s leaf extract fraction toward prolactin level and neuralgia cells in mice is not available recently. The purpose of this study is to find out the influence of giving katu’s leaf extract fraction toward prolactin level and neuralgia cells at mice, and influence prolactin level in breast feeding mouse towards amount of neuralgia cells in mice. Method of this study is laboratories experiment ”the post test-only control group” type experimental animals is mouse (Rattus norvegicus). The samples are 24 breast feeding mice with body weight 150-200 gram and divided into 4 groups, that are control group and treatment group which is given katu’s leaf extract fraction. Treatment group 1 with the dosage 24 mg, treatment group 2 with the dosage 48 mg and treatment group 3 with the dosage 72 mg. Treatment begun at the 3rd day post delivery until the 11th day. The 12th day the mouse is killed, blood from pericardium taken for prolactin test, while the mice is killed and it’s brain made as substance for testing of amount of neuralgia cells. Study result of prolactin with One Way Anova got significant difference (ρ=0,000). Continued with Post Hoc test got significant difference at C–T2, C–T3, T1–T2, T1–T3, and T2–T3. Result of neuralgia cells test at One Way Anova got significant difference (p=0,000).  Continued with Post Hoc test got significant difference at C–T2, C–T3, T1–T2, T1–T3, and T2–T3. Result of linier regression test got ρ (0.940) > α (0,05), means there is no influence prolactin level in breast feeding mouse towards amount of neuralgia cells in mice. The conclusion of the study was prolactin level in breast feeding mouse and amount of neuralgia cells in mice after giving katu’s leaf extract fraction have prolactin level higher and amount of neuralgia cells increase at the dosage 48 and 72 mg. If this study will be applied to human, should be done laboratorial test. More study about containing of breast milk after giving katu’s leaf extract fraction need to be continued.

2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.


2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly


2019 ◽  
Vol 6 (3) ◽  
pp. 195-198
Author(s):  
Dwi Yulinda ◽  
Imroatul Azizah

Background: Breastfeeding is exceptionally superior, giving mother and infants distinct and subtastial physical, mental, and developmental health advantages. Palm fruit has content of phytonutrients with antioxidant properties and galactagogues to help increase their milk supply in the early postpatum days. The lowest exclusive brest feeding rates in DIY is in the city of Yogyakarta. Exclusive breast feeding improves infant immunity so as to minimize the occurrence of diarrhea, constipation, fever that is caused by some allergic reactions. Objectifive: :This study aimed to analyze the relationship between date palm fruits on postpartum to prolactin and volume of breast milk Method: Research was conducted on maternal postpartum which is divided into two groups. They are the control group; and the treatment group which was given dates palm fruit. Breast milk volume and prolactin was measured at 3 day after labor. The analytical method used is Student T-test. Results: Date Palm fruits increased prolactin and volume of breast milk which was significantly on firstweek breastfeeding increase in the treatment group compared to the control group at p <0.05. Conclusion: Date Palm fruits is one of the alternatives that can be done to improve the success of exclusive breast feeding on postpartum. Key Words: Date palm fruit, postpartum, prolactin and volume of breast milk


2018 ◽  
Vol 11 (2) ◽  
pp. 7
Author(s):  
Aulia Wiradi ◽  
Wiwit Ade Fidiawati ◽  
Sri Melati Munir

Paraquat destroys organs including lung. Purpose of this study was to determine the effect of paraquat on histopathologyof lung, exspecially fibrosis in mice (Mus musculus). This study was an experimental study with post-test only withcontrol design and using 15 mice as sample. Samples were divided into 5 groups consist of one control group and fourdifferent doses of paraquat treatment group (25 mg / kg / day, 50 mg / kg / day, 75 mg / kg / day and 100 mg / kg / day for7 days with spraying). Analysis of the data have used One-Way ANOVA then post-hoc test with tukey to assesseddifferences of each groups. The results showed increasing of lung fibrosis with increasing doses and have significantcorrelations (p = 0.000). In conclusion, there are significant changes in lung histopathology of mice (Mus musculus)after paraquat exposure for 7 days.


2018 ◽  
Vol 6 (2) ◽  
Author(s):  
I Gede Sunarba . ◽  
Dr. I Ketut Sudiana, S.Pd. M Kes . ◽  
Gede Doddy Tisna, M.S., S.Or., M.Or. .

Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan side jump sprint dan scissor jump terhadap peningkatan daya ledak otot tungkai. Jenis penelitian ini adalah eksperimen semu dengan rancangan the non-randomized control group pretest posttest design. Subjek penelitian adalah siswa putra peserta ektrakurikuler bolavoli SMP Negeri 3 Abiansemal tahun pelajaran 2016/2017 berjumlah 45 orang, kemudian dibagi menjadi 3 kelompok dengan menggunakan teknik ordinal pairing, yaitu 15 orang diberikan pelatihan side jump sprint, 15 orang diberikan pelatihan scissor jump, dan 15 orang kelompok kontrol. Daya ledak otot tungkai diukur dengan test vertical jump. Data yang di dapat dianalisis dengan uji F (one way anova) pada taraf signifikansi (α) = 0,05 dengan bantuan komputer program SPSS 16.0. Hasil analisis data dari kelompok perlakuan side jump sprint terjadi peningkatan daya leda kotot tungkai sebesar 0,097, pada kelompok perlakuaan scissor jump sebesar 0,136 dan pada kelompok control sebesar 0,116. Hasil one awy anova variable daya ledak otot tungkai antara kelompok perlakuan dan kelompok kontrol didapat Fhitung 67,628 dan signifikansi 0,000 yang berarti terdapat perbedaan pengaruh pelatihan side jump sprint dan scissor jump terhadap daya ledak otot tungkai. . Berdasarkan hasil uji least significant difference (LSD), kelompok pelatihan side jump sprint lebih baik pengaruhnya sebesar 44,733 dibandingkan pelatihan sciisor jump terhadap peningkatan daya ledak otot tungkai. Disimpulkan bahwa; (1) pelatihan side jump sprint dan scissor jump berpengaruh pada peningkatan daya ledak otot tungkai. (2) terdapat perbedaan pengaruh pelatihan side jump sprint dan scissor jump terhadap peningkatan daya ledak otot tungkai. (3) pelatihan side jump sprint lebih baik dari pada pelatihan scissor jump terhadap peningkatan daya ledakotot tungkai Kata Kunci : side jump sprint , scissor jump, daya ledak otot tungkai. This research aims to know the influence of side jump sprint and scissor jump towards the improvement the explosive power of limp muscle. This research is a quasi experiment with the non-randomized control group pretest post test design. The subjects were students of extracurricular participants of volley ball at SMP Negeri 3 Abiansemal academic year 2016/2017 amounted to 45 people, then divided into 3 groups using ordinal pairing technique, 15 people were given side jump sprint training, 15 people were given scissor jump training, and 15 People control group. Limb muscle explosive power is measured by a vertical jump test. The data can be analyzed by F (one way anova) test at significance level (α) = 0,05 with computer program of SPSS 16.0. The result of data analysis from side sprint treatment group happened increased limb muscle explosive power of 0.097, in scissor jump treatment group 0.136 and control group 0.116. The result of one way anova variable of explosive muscle limb power between treatment group and control group was obtained Fcount 67,628 and significance 0,000 which mean there is difference of influence of training of side jump sprint and scissor jump on explosive power of limb muscle. . Based on the least significant difference (LSD) test results, the side jump sprint training group had a better effect of 44.733 than the scissor jump training on increasing the muscle limb power. It was concluded that; (1) the training of side jump sprints and scissor jumps has an effect on increasing the muscle tone of the leg muscles. (2) there is a difference in the effect of training of side jump sprint and scissor jump on the increase of explosive power of limb muscle. (3) side jump sprint training is better than scissor jump training on increasing the limbs . keyword : side jump sprint, scissor jump, explosive muscle limb power.


2022 ◽  
Vol 21 (1) ◽  
pp. 175-183
Author(s):  
- Pujiati ◽  
Dono Indarto ◽  
- Susilorini ◽  
Diani Retno Widyatuti ◽  
Haneda Ilzafira Damayanti ◽  
...  

Background: Asthma is a disease of the respiratory tract in the form of chronic inflammation. Chronic inflammation is one of them characterized by the remodeling of the airways mediated by the anti-inflammatory cytokine TGF-β. In addition there are also several immune cells that play a role such as macrophages, dendritic, neutrophils as producers of IL-12. The presence of the gut-lung axis allows the spread of inflammatory cytokines from the lungs to the intestines and vice versa. Lactobacillus brevis and Leuconostoc mesenteroides have the potential to modulate the immune system through its colonization of the gut. The study aimed to look at the effect of probiotics combined Lactobacillus brevis and Leuconostoc mesenteroides on the expression of TGF-β and IL-12 in the asthma model mouse ileum. Method: This experimental post-test only control group design study used 18 Sprague-Dawley mice. The mice were divided into 3 groups at random: control group (KI), asthma treatment (KII), asthma treatment with a combination of Lactobacillus brevis and Leuconostoc mesenteroides (KIII). Sensitization of asthma with OVA through intraperitoneal injection on days 0 and 14 and through inhalation on days 21 -63. Administration of combination probiotics per oral per day on days 21-63 after inhalation of OVA. The ileum network was taken on the 64th day and measured the expression of TGF-βand IL-12 with immunohistochemical methods and analyzed the percentage proportion of TGF-βand IL-12. Data analysis were conducted by One way ANOVA test and continued post hoc tests. Results: The percentage calculation of the proportion of TGF-βin thecontrol group (KI), asthma group (KII), and the combination group of L.brevis and L.mesenteroides (KIII) is 22.4 ± 15.27; 1.6 ± 2.12; 19.4 ± 6.65. While the percentage calculation of il-12 proportion in each group consecutively were 24.3 ± 19.67; 64.63 ± 9.90; 51 ± 16.62. One way ANOVA Test results on the percentage proportion of TGF-βhave a p value of 0.013 (p<0.05) which means there is a significant difference in the entire group. Furthermore, the Post Hoc Games-Howell test was conducted and obtained a p value of 0.003 (p<0.05) between the asthma group (KII) and the combination group of L.brevis and L.mesenteroides (KIII) which means there are significant differences between the two groups. One way ANOVA Test results on the percentage proportion of IL-12 have a p value of 0.011 (p<0.05) which means there is a significant difference in the entire group. Furthermore, the Post Hoc LSD test was conducted and obtained a p value of 0.001 (p<0.05) between the control group Post Hoc analyses was conducted and obtained a p value of 0.002 (p<0.05) with number of errors was 11.353 between the control group (KI) and asthma group (KII) which means there are significant differences between the two groups. Conclusion: The probiotic administration of lactobacillus brevis and Leuconostoc mesenteroides had no effect on the expression of TGF-β and had no effect on il-12 expression in the asthma model mouse ileum. It is necessary to do research on mice with asthma using combinations with other bacteria in order to get maximum effect. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 175-183


Author(s):  
P. A. Uikey ◽  
Palak Agrawal ◽  
Surekha Khandale

Background: Breast milk contains balanced nutrients for the complete growth of body and brain of neonates and infants. Breast feeding is rich in anti-infective factors and improves immune system, thus reducing the child mortality rate. Objective of present work was to study the effect of antenatal breast milk expression at term in improving lactational performance as compared to control group.Methods: The prospective, comparative study was conducted for 2 years to observe effects of antenatal expression of breast milk on postnatal lactational performance in a tertiary care centre   in Central India.  200 Subjects after 37 weeks of pregnancy divided in two groups of 100 each 1) study group 2) control group by systematic random sampling technique. Prior examination was done to exclude any inverted or cracked nipples and appropriate treatment instituted. The women with diseased breast and conditions, high risk pregnancy, congenitally anomalous fetus and drugs affecting lactation were excluded from study. Study group women were counselled to express milk 2-3 times every day preferably during bathing. All women were followed up in hospital and postnatal lactation performance was observed in both groups.Results: The study group did not find it difficult to initiate breast feeding after vaginal or caesarean delivery  and in 78% of patients time interval from initiation of lactation to establishment of lactation was less than half hour as compared to control group (38%) P value <0.001. Statistical analysis showed significant difference in the results of those groups.Conclusions: Daily antenatal breast milk expression after 37 weeks of pregnancy significantly reduced the time for establishing full breast feeding and reduced breast feeding failures.


e-GIGI ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Fransiska M. Pardosi ◽  
Darmawati A. Indraswari ◽  
Lusiana Batubara ◽  
Nadia Hardini

Abstract: Nanofiller composite resin has small filler size which enhances its properties. However, these properties could decrease due to several factors. Acidic liquid such as coffee could reduce the hardness of composite resin. This study was aimed to analyze the effect of robusta and arabica coffee immersion on the hardness of nanofiller composite resin. A total of 27 composite resin samples were used and then were polished by using a soflex disc for 30 seconds on each roughness level. Samples were then divided into three groups, as follows: the control group with artificial saliva and the treatment groups with robusta coffee and arabica coffee. The composite resin molds were immersed in the three groups for 5 days. After five days of immersion, the hardness of the sample was tested by using a Vickers hardness tester. Artificial saliva as the control group had the highest mean hardness value of 112.98±8.67 VHN, followed by robusta coffee, and then by arabica coffee. The One Way Anova and post hoc LSD test showed that there were significant differences in all groups (p<0.05) except for the two treatment groups, namely robusta and arabica coffee groups which did not show any significant difference in the resin hardness (p>0.05). In conclusion, robusta and arabica coffee affect the hardness of the nanofiller composite resin, but there is no significant difference in the hardness between the robusta and arabica coffee groups.Keywords: composite resin hardness, nanofiller, robusta coffee, arabica coffee Abstrak: Resin komposit nanofiller memiliki ukuran filler kecil yang dapat meningkatkan fungsinya namun fungsi tersebut dapat menurun oleh beberapa hal. Cairan asam yang dikonsumsi seperti kopi dapat menurunkan kekerasan resin komposit. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman kopi robusta dan arabika terhadap kekerasan resin komposit nanofiller. Sampel resin komposit yang digunakan sebanyak 27 buah dan dilanjutkan dengan pemolesan menggunakan soflex disc selama 30 detik setiap tingkat kekasarannya. Sampel kemudian dibagi dalam tiga kelompok, yaitu kelompok kontrol dengan saliva buatan dan dua kelompok perlakuan dengan kopi robusta dan arabika. Sebanyak 27 buah resin komposit direndam ke dalam tiga kelompok tersebut selama lima hari kemudian diuji kekerasannya dengan vickers hardness tester. Saliva buatan sebagai kelompok kontrol memiliki nilai rerata kekerasan tertinggi sebesar 112,98±8,67 VHN, diikuti kopi robusta, dan nilai terendah yaitu kopi arabika. Hasil uji One Way Anova dan post hoc LSD menunjukkan terdapat perbedaan bermakna pada semua kelompok (p<0,05) kecuali pada kedua kelompok perlakuan yaitu antara kopi robusta dan arabika tidak menunjukkan adanya perbedaan kekerasan yang bermakna (p>0,05). Simpulan penelitian ini ialah kopi robusta dan arabika memengaruhi kekerasan resin komposit nanofiller namun tidak terdapat perbedaan kekerasan antara keduanya.Kata kunci: kekerasan resin komposit, nanofiller, kopi robusta, kopi arabika


2018 ◽  
Vol 50 (3) ◽  
pp. 149
Author(s):  
S. Soekobagiono ◽  
Adrian Alfiandy ◽  
Agus Dahlan

Background: Preservation of sockets is a procedure aimed to reduce bone resorption after tooth extraction. One of the most commonly used xenograft materials is demineralized freeze-dried bone bovine xenograft (DFDBBX). Meanwhile, one of the key regulations in osteoclast genesis process is RANKL bond. A decrease in the number of RANKL expressions can suppress the osteoclast genesis process so that bone resorption can be prevented. The combination of Moringa leaf extract and DFDBBX, as a result, is expected to decrease the number of RANKL. Purpose: This study aimed to measure RANKL expressions in tooth extraction socket treated with Moringa leaf extract combined with DFDBBX. Methods: Fifty six Cavia cobaya rats were divided into eight groups. The first group was a control group with PEG administration onto their extraction sockets. The second group was a treatment group with DFDBBX administration. The third group was a treatment group with Moringa leaf extract administration. The fourth group was a treatment group induced with a combination of DFDBBX and Moringa leaf extract. Examination then was performed on days 7 and 30. After 7 and 30 days, those Cavia cobaya rats were executed and tested with immunohistochemical techniques. Further research data collected then were tested with one-way ANOVA. Results: There were significant differences between the control group and the groups induced with the combination of Moringa leaf extract and DFDBBX. On days 7 and 30, the groups induced with the combination of Moringa leaf extract and DFDBBX had the lowest number of RANKL expressions. Conclusion: The combination of Moringa leaf extract and DFDBBX can decrease the number of RANKL expressions in Cavia cobaya rats on the day 7 and day 30 after tooth extraction.


2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Siti Nurjanah ◽  
Nurul Kamariyah ◽  
Umdatus Soleha

Breast milk contains more than a hundred kind of nutrient that needed for baby’s growth and development. But now theres so many mother that didn’t give breast milk because they didn’t produce enough. One of the method that can increase the poduction of breast milk is with giving the breast feeding mother katu’s leaf. This research head for analyze influence of consumption the leaf of Sauropus Androgynus (L) Merr (Katu) that related with raising prolactin hormones of the breast feeding mother and babies development in Wonokromo Surabaya district. This research is Quasi Experiment research with using the post test-only control group design. The population that being the target for this research is all of the breast feeding mother that consume katu’s leaf as many as 18 and 6 people as control. The research unit as many as 24 respondent obtained with using simple random sampling technique. Free variable is extract of katu’s leaf and dependent variabel is prolactin hormones and baby’s development. Collect the data with using check list, and examination of blood samples with pra screening of development questionnaire Result of One Way Anova test between consume katu’s leaf extract with the level of prolactin hormones obtained a significant difference (p = 0,000). The conclusion of this research is the treatment 2 and 3 have the significant result. To increase the production of breast milk and baby’s development maximally the breast feeding mother be expected to consume the katu’s leaf 2 until 3 times a day.


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