scholarly journals Induced Resistance against Fusarium Wilt Disease caused by Fusarium oxysporum in Local Red Peppers (Capsicum annum L.) in Aceh using Rhizobacteria Isolates

2019 ◽  
Vol 2 (1) ◽  
pp. 6
Author(s):  
Siti Hafsah ◽  
Anita Rahayu ◽  
Syamsuddin Syamsuddin ◽  
Firdaus Firdaus

The ability to develop resistance has been demonstrated by Rhizobacteria in several plant species in order to providing protection against plant pathogen attacks. The use of Rhizobacteria is expected to suppress the attack of Fusarium pathogen in local pepper plants in Aceh. This study is aimed at obtaining information about the important role of Rhizobacterium in inducing the resistance of local pepper plants in Aceh to the attack of Fusarium oxysporum. The research was conducted from March to September 2018 at Seed Science Laboratory and Experimental Garden of the Faculty of Agriculture, Syiah Kuala University. The study used Completely Randomized Design (CRD) Factorial with 2 factors. The first factor was the local peppers (Odeng, Lamando Lapaben, Super Lamando 002 and Lanyoe SS). The second factor was Rhizobacteria isolates (HWI 5 (4) and HWI 8 (6)) which were obtained from the collection of Seed Science Laboratory. The parameters observed: pathogen incubation period, plant height, stem diameter and number of productive branches. The results revealed that the incubation period of F. oxysporum varied in each genotype and type of Rhizobacteria. The application of Rhizobacteria is slowing down the incubation period to 1-3 days in all tested genotypes. Rhizobacteria increased the plants growth especially in HWI 8 (6), characterized by taller plants and larger trunks. The highest interaction between the local genotype of pepper plants and the type of Rhizobacteria was found in the genotypes of Lamando Lapaben and HWI 5 (4) for its number of productive branches.

2020 ◽  
Vol 3 (2) ◽  
pp. 70-79
Author(s):  
Frara Efeseli Fakhdian ◽  
Abduh Ulim ◽  
Tjut Chamzurni

Abstrak:Cabai merah (Capsicum annum L.) merupakan salah satu komoditas hortikultura unggulan terbesar dari golongan sayur-sayuran karena memiliki harga jual yang tinggi. Disamping itu selain harga yang tinggi, di lapangan cabai merah sering  mengalami gangguan dari mulai benih disemai sampai tanaman cabai menghasilkan, hal ini disebabkan penyakit tular tanah atau pun terbawa benih. Salah satu penyakit yang memprihatinkan pada tanaman cabai merah adalah penyakit layu yang disebabkan oleh jamur Fusarium  oxysporum f.sp capsici,penyakit ini sering merugikan para petani, jika tanaman sudah terserang oleh penyakitini,  kerugian bisa mencapai 80 % pada tanaman cabai merah. Salah satu alternatif  yang digunakan dalam Pengendalian Hama dan Penyakit terpadu (PHPT) dengan menggunakan agensia Trichoderma formulasi pelet. Penelitian ini bertujuan agar mengetahui keefektifan Trichoderma  Formulasi pelet dalam menekan penyakit Layu Fusarium  yang disebabkan oleh Jamur Fusarium  oxysporum f.sp capsici. Penelitian ini telah dilaksanakan di laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman dan Rumah Kasa Kebun Percobaan Fakultas Pertanian Unsyiah. Penelitian ini dimulai sejak bulan Agustus sampai Oktober 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial dengan 9 perlakuan dan 3 ulangan setiap perlakuan terdiri dari 5 unit polibag sehingga terdapat 135 unit polibag. Peubah yang diamati meliputi masa inkubasi, tinggi tanaman, jumlah daun dan persentase serangan.Hasil penelitian menunjukkan bahwa aplikasi pelet Trichoderma harzianum 2,5 g/polibagdan Trichoderma virens 2 g/polibag mampu memperlama masa inkubasi (Tidak terdapat gejala). Aplikasi pelet  T. virens 1,5 g polibag dapat meningkatkan tinggi tanaman cabai merah sampai 16, 80 cm. Aplikasi pelet T. harzianum  1,5 g/polibag dapat mamacu pertumbuhan  jumlah daun sebesar 9,07 helai. Aplikasi pelet T. harzianum 2,5 g/polibag dan T. virens formulasi pelet 2 g/polibag mampu menekan persentase serangan jamur Fusarium  oxysporum f.sp capsici sebesar 13,68 % pada 35 hari setelah tanam.Combination of Several Doses and Species of Trichoderma Pellet Formulations In Suppressing Development Fungus of fusarium oxysporum f.sp capsici in Red Chili Breeding (Capsicum annum L.)Abstract : a red chili (Capsicum annum L.) is one of the largest superior horticultural commodity of vegetables, because it has a high selling price. Although it has high price, but in the field red chilli often disturbance from the seed sowing until the pepper plants produce, it is cause by soil contagion or even carried by seed. One of the most common diseases in red chili plants is the wilting disease cause of the fungus Fusarium oxysporum f.sp capsici. It often detrimental of the farmers if the plants have been attacked by Fusarium wilt disease, the loss could reach out about 80% in red chili plants. One of the alternative to Integrated Pest and Disease Control (PHPT) is using the Trichoderma pellet formulation agent. The reasearch purpose to determine of the effectiveness Trichoderma Formulation of pellets in suppressing Fusarium Lung disease caused by Fusarium oxysporum f.sp capsici. The research has been conducted in Plant Disease Laboratory of Plant Protection Study Program and home srceen Experimental Garden of Agriculture Faculty Unsyiah. The research was started since August to October 2017. The research uses Completely Randomized Design (RAL) non factorial pattern with 9 treatments and 3 replications each treatment consists of 5 units of polybags so that there are 135 units of polybags. The variable observed included incubation period, plant height, number of leaves and percentage of attacks. Results of the research showed that the application of Trichoderma harzianum pellet 2.5 g / polybag and Trichoderma virens 2 g / polybag was able to prolong the incubation period. Application of T. virens pellet 1.5 g polybags can increase the height of red pepper plants up to 16, 80 cm. Application of pellets T. harzianum 1.5 g / polybags can spure growth of leaves amounted to 9.07 strands. The application of T. harzianum pellet 2.5 g / polybag and T. virens pellet 2 g / polybag formulation was able to suppress the percentage of Fusarium oxysporum f.sp capsici attack by 13.68% at 35 days after planting.


2019 ◽  
Vol 2 (2) ◽  
pp. 52-58
Author(s):  
Tati Hariyati ◽  
Fajwati Fajwati

This study aims to determine the effect of growth regulators Hormonik against Parthenocarpy process on three major varieties of pepper plants and to determine the effect of plant growth regulator that is optimum for the process Hormonik Parthenocarpy in three major varieties of chili. This research was conducted in Tanjung Selor, in January to May 2016. The design used in this study is completely randomized design (CRD) with two factors and nine replications. The first factor is the treatment Hormonik consisting of four levels ie 0 cc, 1 cc, 2 cc, 3 cc, the second factor is the variety Arielle, Darmais and Pilar. From the analysis of variance showed that the treatment Hormonik to varieties of very significant effect on the parameters of heavy fruit crop, the number of fruit crops, fruit length persampel and the number of seeds persampel first harvest, after a further test using LSD 5%, by weight of fruit crops and the amount of fruit planting the first crop was not significantly different, but significantly different with persampel fruit length and number of seeds persampel first harvest.


2017 ◽  
Vol 4 (1) ◽  
pp. 31
Author(s):  
Uswatun Hasanah ◽  
Purnomowati Purnomowati ◽  
Uki Dwiputranto

Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.


2020 ◽  
Vol 3 (01) ◽  
pp. 28-39
Author(s):  
Sri Wilarso ◽  
Putri Maharani ◽  
Andi Sukendro ◽  
Cahyo Wibowo

Problems arising from silica sand mining are decreasing soil fertility, as well as the presence of high heavy metals such as Fe and Al, which inhibits the growth of revegetation plants. The purpose of this study was to analyze the response of the growth of balsa seedlings (Ochroma bicolor Rowlee.) on silica sand post-mining media treated with MycoSilvi, compost, and lime and determine the most optimal combination of MycoSilvi, compost, and lime treatment. This study uses a completely randomized design (CRD) with a split-plot design with 3 treatment factors, namely the addition of MycoSilvi, compost, and lime. Each treatment consisted of five replications. The results showed that the planting media that were not treated (control) was not able to support the growth of balsa seedlings. The interaction of MycoSilvi, compost, and lime significantly affected the height, diameter, biomass, and percentage of mycorrhizal colonization parameters, and significantly affected the plant chlorophyll content. The combination of MycoSilvi type 1 treatment, namely Glomus mosseae and lime (C0K1M1) type, resulted in the highest total growth rate, diameter, biomass, and colonization percentage compared to other treatments. The addition of lime and compost to the growth medium can reduce the degree of mycorrhizal dependency of balsa seedling.


SIMBIOSA ◽  
2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Fenny Agustina ◽  
Agam Akhmad Syaukani ◽  
Fatimah Sahara

The study was conducted over two months, from March to April 2012. This study aims to analyze the influence of type of packaging materials for durability of chilli (Capsicum annum L.) during cold storage. The method used in this study is an experimental method using a completely randomized design (CRD), which consists of four types of packaging materials, namely of chilli are packed in plastic PE, PP, PS, PS + control film with a red pepper that is stored without packaging. The parameters analyzed are severe shrinkage and the organoleptic (texture, color, aroma and overall). From the test results obtained ANAVA Fhitung value of 203,869 is greater than the value Ftabel, both at the level of 5% for 3,480 and 5,990 at 1% level. For the organoleptic value of PS + plastic film gives the highest yield in each test the texture attributes of 6,678. 6,500 color, aroma and overall 6,122 and 6,722. PP plastic gives the lowest result in each test the texture attributes of 3,156, 3,556 color, aroma and overall 3,333 and 3,300, this indicates that there is a very significant effect between the type of packaging materials for durability chili during cold storage.


2020 ◽  
Vol 13 (1) ◽  
pp. 123-131
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.


2001 ◽  
Vol 1 (2) ◽  
pp. 60-62
Author(s):  
Titik Nur Aeny

ABSTRACTPathogenicity of banana wilt  bacteria (Ralstonia sp.) on  several other  plants.  This experiment was conducted to investigate the pathogenicity of bacterial wilt of banana pathogen (Ralstonia sp.) isolated from infected plants in Lampung on tomato, chili pepper, eggplant, and ginger.  The experiment was conducted in Laboratory of Plant Protection Department,  Faculty of Agricultural University of Lampung, from May 1999 to February 2000.  Treatments in this experiment were arranged in a completely randomized design with four replications. The treatments were tomato, eggplant, chili pepper, and  ginger.  Banana was used as the control. The results of this experiment showed that up to 30 days after inoculation, the bacteria isolated from banana found  in Lampung was not able to cause symptom on tomato,  chili pepper, eggplant, nor ginger. However, this bacteria caused wilting on banana eight days after inoculation; the incubation period of this bacteria on banana was eight days. 


2016 ◽  
Vol 5 (4) ◽  
pp. 285-292
Author(s):  
Mia Arista Sari ◽  
Pujiono Wahyu Purnomo ◽  
Haeruddin Haeruddin

ABSTRAK Salah satu peran penting mangrove bagi kehidupan biota akuatik adalah sebagai penyedia nutrien dari bahan organik yang dihasilkan. Proses dekomposisi bahan organik membutuhkan oksigen dan bantuan bakteri. Apabila kadar oksigen berkurang maka dapat mempengaruhi proses dekomposisi dan akhirnya dapat berpengaruh terhadap kehidupan bentos yang ada. Tujuan dari penelitian adalah  mengetahui kebutuhan oksigen yang digunakan dalam proses dekomposisi bahan organik pada kawasan mangrove. Penelitian bersifat eksperimental terhadap deposit bahan organik. Penelitian mengacu pada rancangan acak lengkap dengan 3 perlakuan (deposit organik mangrove padat, sedang, dan jarang) yang masing – masing diulang 3 kali. Penelitian dilakukan di kawasan mangrove desa Bedono, Demak. Peubah utama yang diukur adalah Disolved Oxygen, bahan organik sedimen secara berkala pada jam ke- 0, 12, 24, 36, 48, 60 dan 66. Peubah tambahan yang diukur adalah kelimpahan bakteri. Diukur penurunan Disolved Oxigen pada waktu tertentu. Diukur pula nilai bahan organik sedimen dan kelimpahan koloni bakteri. Hasil yang didapat yaitu nilai kebutuhan oksigen sedimen tertinggi pada stasiun 1 dengan kisaran 1,3 – 3,5. Nilai kebutuhan oksigen sedimen pada stasiun 2 berkisar antara 1,2 – 2,2 dan nilai kebutuhan oksigen sedimen pada stasiun 3 berkisar antara 0,8 - 2,7. Hasil kelimpahan koloni bakteri pada stasiun 1 berkisar antara 1 x 1011 – 1,5 x 1011Cfu/ml. Stasiun 2 berkisar antara 2 x 1011 - 22 x 1011Cfu/ml dan stasiun 3 berkisar antara 1 x 1011 - 2 x 1011Cfu/ml. Kualitas perairan seperti temperatur berikisar antara 27 - 32 ºC. Nilai salinitas beriksar antara 25 - 29‰ dan nilai pH berkisar antara 7 - 8. Kata kunci: Oksigen; Bahan Organik; Sedimen; Dekomposisi ABSTRACT One important role of mangrove for aquatic biota is a provider of nutrients from organic matter produced. The process of decomposition of organic matter need oxygen and help the bacteria. When oxygen levelsdecreases, it can affect the decomposition process and can ultimately affect the existing benthic life. The purpose of the research was to determine the need of oxygen used in the decomposition of organic matter in the mangrove areas. It is a experimental research on the deposit of organic material. The research refers to a completely randomized design with 3 treatments (dense mangrove organic deposits, medium, and rare) that eachs repeated three times. Research conducted in the mangrove areas Bedono village, Demak. The main variables measured were Disolved Oxygen, sedimentary organic matter on a regular basis on the hour 0, 12, 24, 36, 48, 60 and 66. Additional variables that measured the abundance of bacteria.MeasuredDisolvedOxigen decline at a certain time. Measured the value of sedimentary organic matter and the abundance of bacterial colonies. The result is the highest value of the oxygen requirement of sediment at station 1 is in the range of 1,3 to 3,5. The value of the oxygen requirement of sediment at station 2 ranged from 1,2 to 2,2 and the value ofsediment oxygen demand of the three stations ranged from 0,8 to 2,7. The result of the abundance of bacteria colonies from the at station 1 range from 1 x 1011 to 1,5 x 1011Cfu/ml. Station 2 ranging from 2 x 1011 -22 x 1011Cfu/ml and three stations ranged between 1 x 1011 -2 x 1011Cfu/ml. Water quality  such as temperature ranged from 27 - 32 ºC. Salinity value ranged from 25 - 29 ‰ and pH values ranged from 7-8.                                                                                                                              Keywords: Oxygen; Organic Matter; Sediment; Decompotition


2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Hapsoh ◽  
Wawan ◽  
Isna Rahma Dini ◽  
Devi Andriani

This study aims to test the bio-fertilizer formulation with Bacillus cereus bioactivator and to obtain the best bio-fertilizer formulation for the growth and yield of upland rice (Oryza sativa L.) in peat medium. The research was conducted in the experimental garden and Soil Science Laboratory, Faculty of Agriculture, University of Riau from July to October 2018. The study was carried out experimentally using a completely randomized design (RAL) with the treatment tested was the formulation of Bacillus cereus (F): F0: 100 ml Bacillus cereus without formulation, F1: 100 ml Bacillus cereus inoculant+74% bagasse+13% dolomite+13% zeolite, F2: 100 ml Bacillus cereus inoculant+74% rice husk+13% zeolite+13% dolomite, F3: 100 ml inoculant Bacillus cereus+74% solid+13% dolomite+13% zeolite, F4: 100 ml Bacillus cereus inoculant+74% tkks+13% zeolite+13% dolomite. The data obtained were analyzed statistically using variance fingerprints. The average result of the analysis was continued with the BNJ test at the 5% level. The results showed that administration of Bacillus cereus without formulation and with the formulation of solid organic matter of rice husks, solids and oil palm empty bunches showed a good response to the amount of chlorophyll and yield of upland rice plants.


2019 ◽  
Vol 18 (2) ◽  
pp. 169
Author(s):  
Selvi Helina ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Andi Trisyono

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.


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