scholarly journals Inflammatory responses, energy metabolism enzymes, oxidative status in Clostridium perfringens infection in broilers

2020 ◽  
Vol 9 (11) ◽  
pp. e4969119320
Author(s):  
Gabriela Miotto Galli ◽  
Marcel Manente Boiago ◽  
Carine Freitas de Souza ◽  
Lorenzo Binotto Abbad ◽  
Matheus Dellamea Baldissera ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Clostridium Perfringens ◽  
Energy Homeostasis ◽  
Inflammatory Responses ◽  
Basal Diet ◽  
Inflammatory Cells ◽  
Experimental Period ◽  
Feed Conversion ◽  
Oxidative Stress Biomarkers ◽  
Metabolism Enzymes

The aim of this study was to determine whether infection by Clostridium perfringens negatively interferes with the oxidant/antioxidant status and activity of energy metabolism enzymes (creatine kinase (CK), adenylate kinase (AK) and pyruvate kinase (PK)), as well as the zootechnical performance of broilers. A completely randomized design with three treatments, with five replications by treatment, and 10 birds by repetitions was used: T1: non-infected group; T2: group infected with C. perfringens; T3: group infected with C. perfringens, and basal diet with performance enhancers (antibiotic and coccidiostatic). At 21 days of age, the birds were experimentally infected orally with 4.0 x 108 CFU/mL C. perfringens. At strategic moments during the experimental period, zootechnical data and blood samples were collected. There were no significant differences among treatments in terms of weight gain, feed intake, or feed conversion. At 20 days, heterophils counts were significantly lower in the T3 group birds than in the others. A significant reduction in the number of total leukocytes, because of the lower number of lymphocytes, was observed in groups T2 and T3 on day 34. Neutrophil and monocyte counts were significantly lower in group T3 than in group T1 on day 34. On day 42, there were significantly lower levels of total protein and globulin in birds in T3 than in T1. On day 27, PK activity was significantly higher in groups T2 and T3; similar behavior observed on day 34 when CK and PK activities in T2 and T3 were significantly higher than those of T1. AK activity was significantly higher in the serum of T3 birds than in those of the other groups at 34 days. At 41 days, CK and PK activities were significantly higher only in birds in group T3 compared to other groups. The levels of reactive oxygen species and lipoperoxidation, as well as the activity of glutathione S-transferase, were significantly lower in birds from group T3 compared to other groups at age 20 days. After a challenge with C. perfringens, there were fluctuations in the behavior of oxidative stress biomarkers; in particular, at 41 days of age, birds in T2 had elevated levels of lipoperoxidation, different from what was seen in birds in T3 that consumed feed with a performance enhancer. Preliminary results suggest that clostridiosis affects serum activity of enzymes in the phosphotransfer network, requiring compensatory enzymatic changes in an attempt to maintain energy homeostasis. In addition, the infection reduces the number of inflammatory cells and causes oxidant/antioxidant imbalance that may contribute to the pathophysiology of the disease. Además, la infección reduce el número de células inflamatorias y provoca un desequilibrio en el estado oxidante / antioxidante que puede contribuir a la fisiopatología de la enfermedad.

scholarly journals Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 507 ◽  
Author(s):  
Elsayed O. S. Hussein ◽  
Shamseldein H. Ahmed ◽  
Alaeldein M. Abudabos ◽  
Mashael R. Aljumaah ◽  
Manal M. Alkhlulaifi ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Basal Diet ◽  
Negative Control ◽  
Feed Additives ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Blood Indices ◽  
Blood Constituents ◽  
Glucose Levels ◽  
Notable Increase

This study evaluated the effects of feed additives on the growth, blood biochemistry and intestinal health of broiler chicks. A total of 378 of broiler chicks (Ross 308) were randomly allotted to seven groups. Chicks were fed a basal diet with 0.0 (control negative), 0.0 (control positive), 0.1, 0.5, 0.12, 0.5 + 0.12 and 0.2 g Kg−1 of Maxus, CloStat, Sangrovit Extra, CloStat + Sangrovit Extra and Gallipro Tech, respectively for 35 days. After 15 days, the chicks were inoculated with Clostridium perfringens. All feed additives were found to enhance growth performance and feed efficiency. The best feed conversion ratio was found in the Negative Control, CloStat + Sangrovit Extra and Gallipro Tect groups, respectively. A notable increase in villus length, total villus area, small intestine weight, ilium weight and total lesion score was found in chicks supplemented with Bacillus subtilis. Besides, the dietary inclusion of phytobiotic compounds showed potential in reducing the serum Alanine aminotransferase (ALT) concentration and increasing the glucose levels. All intestine and liver histopathological signs were reduced in chicks fed a probiotic-supplemented diet. Our findings indicate that supplementation with probiotics and phytobiotics alone or in combined form can be used to enhance performance, intestine health and blood constituents against C. perfringens infection in broiler chicks.

scholarly journals Use of different combinations of enzyme complexes in broiler diets

2021 ◽  
Vol 42 (5) ◽  
pp. 2903-2924
Author(s):  
Lindolfo Dorcino dos Santos Neto ◽  
◽  
Julyana Machado da Silva Martins ◽  
Genilson Bezerra de Carvalho ◽  
Roberto Moraes Jardim Filho ◽  
...  
Keyword(s):  
Basal Diet ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Enzyme Complex ◽  
Ether Extract ◽  
Feed Conversion ◽  
Randomized Design ◽  
Final Weight ◽  
Completely Randomized Design ◽  
Enzyme Complexes

Two experiments were carried out to investigate the effect of “on top” addition of different enzyme complexes, the enzyme α-galactosidase and three sources of the enzyme phytase available on the market, in broiler diets. In the first experiment, 1260 one-day-old Cobb 500® chicks were distributed into seven treatments in a completely randomized design (CRD) with six replicates and 30 birds/replicate. Treatments consisted of combinations of different enzyme complexes, namely, complex A (phytase, protease, xylanase, ß-glucanase, cellulase, amylase, pectinase), complex B (protease and cellulase) and complex C (xylanase, amylase and protease); isolated α-galactosidase (GAL); and three sources of phytase (P1, P2 and P3) in the diet. The treatments were formulated as follows: T1 - basal diet (BD); T2 - BD + enzyme complex A + enzyme complex B (BDAB); T3 - BDAB + GAL; T4 - BD + complex A + GAL; T5 - BD + complex C + P1 + GAL (BDCG); T6 - BDCG + P2; and T7 - BDCG + P3. The following variables were measured in the experimental period of 42 days: feed intake (FI), weight gain (WG), average final weight (AFW), feed conversion (FC), and carcass yield. Significant differences occurred for AFW, WG and FC in the pre-starter phase. In the second experiment, 112 Cobb 500® chicks aged 25 days were distributed into seven treatments in a CRD with four replicates and four birds/replicate. Treatments were the same as in the first experiment. Nutrient digestibility was evaluated in an experimental period of seven days. Differences were found in the metabolism coefficient of ether extract (MCEE). Dietary inclusion of enzyme complexes improves the AFW and WG of chickens from 1 to 7 days of age and MCEE in the grower phase.

scholarly journals Administration of Saccharomyces boulardii mafic-1701 improves feed conversion ratio, promotes antioxidant capacity, alleviates intestinal inflammation and modulates gut microbiota in weaned piglets

2020 ◽  
Author(s):  
Wenxiu Zhang ◽  
Chengling Bao ◽  
Jian Wang ◽  
Jianjun Zang ◽  
Yunhe Cao
Keyword(s):  
Intestinal Inflammation ◽  
Inflammatory Responses ◽  
Animal Health ◽  
Basal Diet ◽  
Saccharomyces Boulardii ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Weaned Piglets ◽  
Weaned Piglet

Abstract Background: Probiotics for are used as a means to improve animal health and intestinal development. Saccharomyces boulardii (S. boulardii) is a well-known probiotic, however, few studies have examined the effects of S. boulardii on weaned piglet performance. Therefore, this 28-day study compared the effects of S. boulardii mafic-1701 and aureomycin in weaned piglet diets on growth performance, antioxidant parameters, inflammation and intestinal microbiota. One hundred and eight weaned piglets were randomly divided into three dietary treatment groups: (1) basal diet (CON); (2) basal diet supplemented with 75 mg/kg aureomycin (ANT); (3) basal diet supplemented with 1 × 108 CFU/kg S. boulardii mafic-1701 (SB). Results: Compared to CON group, SB group improved feed efficiency over the entire 28 days (P < 0.01) and decreased the rate of diarrhea during the first two weeks (P < 0.05). Total superoxide dismutase concentration was markedly increased in SB group (P < 0.05). Moreover, compared with CON group, SB group decreased the level of pro-inflammatory cytokines interleukin-6 (P < 0.01) and tumor necrosis factor (P < 0.05) in jejunum. Supplementation with S boulardii mafic-1701 increased abundance of Bacillus and Ruminococcaceae (P < 0.05), whereas abundance of Clostridiaceae was decreased (P < 0.05). Furthermore, S boulardii mafic-1701 administration increased cecal concentration of microbial metabolites, isobutyrate and valerate (P < 0.05). Conclusion: The improvement in feed conversion ratio, reduction in diarrhea rate during the first two weeks in weaned piglets provided diets supplemented with S. boulardii mafic-1701 may be associated with enhanced antioxidant activity, anti-inflammatory responses and intestinal microbial ecology.

scholarly journals Growth performance and antioxidant status of growing rabbits fed on diets supplemented with Eucommia ulmoides leaves

2018 ◽  
Vol 26 (1) ◽  
pp. 35 ◽  
Author(s):  
Song Li ◽  
Miaoqing Zhao ◽  
Tingting Jiang ◽  
Wenwen Lv ◽  
Shujuan Gao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Intake ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Eucommia Ulmoides ◽  
Feed Conversion ◽  
Diet Control ◽  
And Control

<p>The present study evaluated the effect of dietary <em>Eucommia ulmoides leaves</em> (EUL) on growth performance and antioxidant status of growing rabbits under heat stress condition. Four hundred and fifty weaned New Zealand male rabbits (6 wk old) were randomly divided into 3 equal groups (150 rabbits/group) and fed with a basal diet (control, digestible energy (DE): 15.92 MJ/kg and crude protein (CP): 19.24%) or the basal diet supplemented with 1 or 5 g of EUL/kg of diet (EUL1 and EUL5), in which the same quantity of barley meal was replaced. During the 21 d of experiment (43 to 63 d of age), the temperature and relative humidity of the rabbit house ranged from 27.5 to 32.5°C and from 65 to 73%, respectively. We analysed feed intake, growth performance and antioxidant status of growing rabbits. Compared with the control group, at the end of the experimental period, EUL supplementation significantly reduced the average daily feed intake (92.0, 92.8 and 100.7 g/d for EUL1, EUL5 and control, respectively; P&lt;0.05), improved the feed conversion ratio (3.80, 3.81 and 4.59 for EUL1, EUL5 and control, respectively; P&lt;0.05), increased the activities of glutathione peroxidase (+35.5 and +35.0% in plasma and liver of rabbits in EUL5 vs. control group, respectively; P&lt;0.05) and reduced those of malondialdehyde (–12.0 and –46.0% in plasma and liver of rabbits in EUL5 vs. control group, respectively; P&lt;0.05). These results suggest that inclusion of EUL in the diet of growing rabbits improved the growth performance and antioxidant status in growing rabbits.</p>

scholarly journals Impact of zinc oxide, benzoic acid and probiotics on the performance and cecal microbiota of piglets

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Caio Abercio da Silva ◽  
Leonardo Aparecido Teixeira Bentin ◽  
Cleandro Pazinato Dias ◽  
Marco Aurélio Callegari ◽  
Vitor Barbosa Facina ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Benzoic Acid ◽  
Early Stage ◽  
Basal Diet ◽  
Experimental Period ◽  
Feed Additives ◽  
Feed Conversion ◽  
Bacterial Fermentation ◽  
Cecal Microbiota ◽  
The Impact

Abstract Background Intestinal health remains a key factor in animal production because it is essential for digestion, absorption and bacterial fermentation. Feed additives have been used to attenuate the weaning stress such as Zinc Oxide (ZnO) and benzoic acid (C7H6O2). The objective of this study was to evaluate the impact of of benzoic acid and probiotics (BA + P) on performance, diarrhea and cecal microbiota of piglets in the nursery phase (23 to 65 days). Results One hundred and sixty weaned piglets with an initial weight of 6.335 ± 0.698 kg and 23 days of age were submitted to four treatments: supplementation with 2500 ppm of Zinc oxide (ZnO), supplementation with a commercial blend of benzoic acid and probiotics (Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium NCIMB 10415; Vevogut P®) (BA + P), supplementation with Zinc oxide plus benzoic acid and probiotics (ZnO + BA + P), and controls receiving only the basal diet without any supplementation. At 65 days of age, 32 piglets (n = 8 per treatment) were slaughtered for the evaluation of the cecal microbiota. Supplementation with ZnO and BA + P were associated with better feed conversion (P < 0.05) in the early stage (23 to 49 days) and with an improvement in all performance parameters over the entire experimental period. The occurrence of diarrhea was lower (P < 0.05) in the BA + P group. The 4 most abundant phyla along with unclassified bacteria represented 93% of all sequences. Firmicutes dominated the cecal microbiota of all groups, followed by Bacteroidetes. Richness represented by the observed number of genera and by the Chao index were statistically lower in ZnO and ZnO + BA + P supplemented animals compared to controls. The beta diversity analysis that compares similarities between bacterial communities demonstrated formation of two distinct clusters containing samples with and without supplementation with ZnO, confirming a strong influence of ZnO on the intestinal microbiota. Conclusion The use of Benzoic acid with probiotics yields similar performance results with lower impact on the gut microbiota compared to ZnO, and it should be considered as a potential alternative in swine production.

scholarly journals Prebiotic and antimicrobials on performance, carcass characteristics, and antibody production in broilers

2016 ◽  
Vol 46 (6) ◽  
pp. 1070-1075 ◽  
Author(s):  
Maíra Fomentini ◽  
Douglas Haese ◽  
João Luís Kill ◽  
Rodrigo Pereira Sobreiro ◽  
Débora Del Puppo ◽  
...  
Keyword(s):  
Antibody Production ◽  
Corn Starch ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Experimental Period ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Randomized Design ◽  
Mannan Oligosaccharides

ABSTRACT: To evaluate the effect of supplementation with mannan oligosaccharides, avilamycin and halquinol, alone or in combination, on the performance, carcass characteristics and antibody production in broilers (1-49 days old), male broiler chicks (n=1440; Cobb 500; one day old) were housed and distributed into a completely randomized design into six treatments (eight replicates; 30 animals per pen). To produce the experimental diets, three types of performance enhancer additives were used. Halquinol (HAL), avilamycin (AVI) and mannan oligosaccharides (MOS) were included (alone or in combination) in the basal diet (instead of corn starch). Effects of diet were observed on results of animal performance in the period 1-21 and 1-42 days old. Broilers fed with a diet without growth promoter showed lower weight gain in relation to those fed with diets with antimicrobials, MOS or a combination of them. In the period 1-49 days old, feed conversion increased in broilers fed with rations without promoter. At the end of the experimental period no influence of diets was observed on the carcass yield and cuts, and titles of specific antibodies to avian infectious bronchitis. The use of MOS and/or antimicrobials (AVI or HAL), alone or in combination, improves feed conversion of broilers reared until 49 days of age.

scholarly journals Effect of a multivitamin complex and probiotic blend in drinking water before and after vaccination on performance traits, blood biochemistry and humoral immune response of broilers

2019 ◽  
Vol 44 (1) ◽  
pp. 28
Author(s):  
N. Jafarpour ◽  
F. Javandel ◽  
S. Gamboa ◽  
A. Seidavi ◽  
V. Tufarelli ◽  
...  
Keyword(s):  
Immune Response ◽  
Drinking Water ◽  
Humoral Immune Response ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Experimental Period ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Humoral Immune ◽  
Before And After

This study was conducted in order to investigate the effects of a multivitamin complex and probiotic blend in drinking water before and after vaccination on growth performance, carcass traits, blood biochemical parameters and humoral immune response of broiler chickens. A total of 150 one day-old male broiler chicks (Ross 308) were randomly allocated to five treatment groups, with three replicates (10 birds per replicate) in a completely randomized design. Experimental treatments consisted of: 1) a basal diet and drinking water without any additives as control (C); 2) C + drinking water + multivitamin complex from 2 days before vaccination until 3 days after vaccination; 3) C + drinking water + multivitamin complex for 3 days after vaccination; 4) C + drinking water + probiotic blend from 2 days before vaccination until 3 days after vaccination; and 5) C + drinking water + probiotic blend for 3 days after vaccination. In the whole experimental period, feed intake, body and thigh weight and abdominal fat were significantly affected (P<0.05) by dietary treatments. On the contrary, no significant effect was observed on carcass characteristics, feed conversion ratio, blood parameters, antibody production against SRBC and IgG. The titer of IgM was higher in treatment (2) than other treatments at 28 d of age (P<0.05). In conclusion, a multivitamin complex supplied in drinking water for 2 days before vaccination till 3 days after vaccination is enable to improve broiler performance and immunity.

scholarly journals Deficiency of Tristetraprolin Triggers Hyperthermia through Enhancing Hypothalamic Inflammation

2021 ◽  
Vol 22 (7) ◽  
pp. 3328
Author(s):  
Da Yeon Jeong ◽  
Nuri Song ◽  
Hye Rim Yang ◽  
Thai Hien Tu ◽  
Byong Seo Park ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Energy Homeostasis ◽  
Target Genes ◽  
Rna Binding ◽  
Inflammatory Responses ◽  
Core Body Temperature ◽  
Inflammatory Protein ◽  
Tightly Coupled ◽  
Hypothalamic Inflammation ◽  
The Stability

Tristetraprolin (TTP), an RNA-binding protein, controls the stability of RNA by capturing AU-rich elements on their target genes. It has recently been identified that TTP serves as an anti-inflammatory protein by guiding the unstable mRNAs of pro-inflammatory proteins in multiple cells. However, it has not yet been investigated whether TTP affects the inflammatory responses in the hypothalamus. Since hypothalamic inflammation is tightly coupled to the disturbance of energy homeostasis, we designed the current study to investigate whether TTP regulates hypothalamic inflammation and thereby affects energy metabolism by utilizing TTP-deficient mice. We observed that deficiency of TTP led to enhanced hypothalamic inflammation via stimulation of a variety of pro-inflammatory genes. In addition, microglial activation occurred in the hypothalamus, which was accompanied by an enhanced inflammatory response. In line with these molecular and cellular observations, we finally confirmed that deficiency of TTP results in elevated core body temperature and energy expenditure. Taken together, our findings unmask novel roles of hypothalamic TTP on energy metabolism, which is linked to inflammatory responses in hypothalamic microglial cells.

scholarly journals PENGARUH PENGGUNAAN LEMAK SAPI, MINYAK KELAPA DAN MINYAK KEDELAI DALAM PAKAN TERHADAP PERFORMA PRODUKSI AYAM PEDAGING

BUANA SAINS ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 1-6
Author(s):  
A. B. A Mardhotillah ◽  
Hariyadi Darmawan ◽  
I. H. Djunaidi ◽  
L. C. Hsia ◽  
Y. C. Chen
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Growth Performance ◽  
Coconut Oil ◽  
Basal Diet ◽  
Experimental Period ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Consumption ◽  
Feed Conversion

This experiment was conducted to evaluate the effect of various fat sources and their combination (coconut oil and soybean oil) on growth performance of broiler. A total of 80 one-day old female 1-d-old Arbor Acres were randomly assigned into four groups (4 treatment) with 10 replicates (6 birds per pen) within 42 days. The experimental groups consisted of four treatment of diets: 1) a basal-diet containing beef oil (BO),  2) a basal diet containing coconut oil (CO), 3) a basal diet containing soybean oil (SO), and 4) a basal diet containing coconut oil and soybean oil (CO+SO) as were used. Performance data were obtained during the feeding period. The result was shown that using combination of medium chain fatty acid and unsaturated fatty acid (CO+SO) were increased growth performance demonstrated by highest bodyweight (59.62 g) compared with other treatments (P 0.05) throughout the experimental period (0-42 days). The lower group for feed conversion ratio was obtained in the diet containing beef oil (BO) compared with other groups (1.32; P 0.05). Feed consumption and bodyweight have significantly different (P 0.05) but not different for feed conversion ratio (P 0.05). It can be concluded that feeding combination CO+SO in the diet may improve the performance of broiler.Beef oil; broiler; coconut oil; growth performance; soybean oil

scholarly journals Administration of Saccharomyces boulardii mafic-1701 improves feed conversion ratio, promotes antioxidant capacity, alleviates intestinal inflammation and modulates gut microbiota in weaned piglets

2020 ◽  
Author(s):  
Wenxiu Zhang ◽  
Chengling Bao ◽  
Jian Wang ◽  
Jianjun Zang ◽  
Yunhe Cao
Keyword(s):  
Intestinal Inflammation ◽  
Inflammatory Responses ◽  
Animal Health ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Saccharomyces Boulardii ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Weaned Piglets

Abstract Background: Probiotics are used as a means to improve animal health and intestinal development. Saccharomyces boulardii ( S. boulardii ) is a well-known probiotic, however, few studies have examined the effects of S. boulardii on weaned pig let performance. Therefore, this 28-day study compared the effects of S. boulardii mafic-1701 and aureomycin in weaned piglet diets on growth performance, antioxidant parameters, inflammation and intestinal microbiota. One hundred and eight weaned piglets were randomly divided into three dietary treatment groups: (1) basal diet (CON); (2) basal diet supplemented with 75 mg/kg aureomycin (ANT); (3) basal diet supplemented with 1 × 10 8 CFU/kg S. boulardii mafic-1701 (SB). Results: Compared to CON group, SB group had higher feed efficiency ( P < 0.01) and lower diarrhea rate ( P < 0.05) over the entire 28 days. Total superoxide dismutase concentration in serum was markedly increased in SB group ( P < 0.05). Moreover, compared with CON group, SB group decreased the level of pro-inflammatory cytokines interleukin-6 ( P < 0.01) and tumor necrosis factor ( P < 0.05) in jejunum. Supplementation with S. boulardii mafic-1701 increased abundance of Bacillus and Ruminococcaceae_UCG_009 ( P < 0.05), whereas abundance of unclassified _Clostridiaceae_4 was decreased ( P < 0.05). Furthermore, S. boulardii mafic-1701 administration increased cecal concentration of microbial metabolites , isobutyrate and valerate ( P < 0.05). Conclusions: The improvement in feed conversion ratio, reduction in diarrhea rate in weaned piglets provided diets supplemented with S. boulardii mafic-1701 may be associated with enhanced antioxidant activity, anti-inflammatory responses and improved intestinal microbial ecology.

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