Monoglyceride Blend Reduces Mortality, Improves Nutrient Digestibility, and Intestinal Health in Broilers Subjected to Clinical Necrotic Enteritis Challenge

This study evaluated the potential of monoglyceride blend (MG) and buffered formic acid (FA) as alternatives to antibiotics in the performance and intestinal health of broilers under clinical necrotic enteritis (NE) challenge. A total of 544 as-hatched Ross 308 broiler chicks were randomly distributed to 32-floor pens housing 17 birds per pen. The four treatments were: NC—non-additive control; ZBS—antibiotic group supplemented with zinc bacitracin and salinomycin; MG—additive MG supplementation in the starter phase only; and MGFA—additive MG in starter phase and FA in grower and finisher phases. All birds were challenged with Eimeria spp. and Clostridium perfringens. Results showed that the NC group had lower BWG and higher FCR than the ZBS group in the grower and overall period (p < 0.05). The NC group had higher NE-caused mortality (days 14 to 17) than the ZBS group (p < 0.05). Birds fed MG had lower NE-caused mortality than the NC group (p < 0.05). Birds fed MG had upregulated jejunal tight junction protein1 (TJP1) and immunoglobulin (IgG) on day 16 and improved gross energy digestibility on day 24 than the NC group (p < 0.05). These findings suggest that supplementation of MG may improve intestinal health and protect birds from clinical NE occurrence.

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PSVI-23 Evaluation of graded levels of Bacillus coagulans (GBI-30, 6086) on apparent nutrient digestibility, stool quality, and intestinal health indicators in healthy adult dogs

Journal of Animal Science ◽

10.1093/jas/skaa278.560 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 314-315

Author(s):

Heather L Acuff ◽

Charles G Aldrich

Keyword(s):

Organic Matter ◽

Fixed Effects ◽

Crude Protein ◽

Healthy Adult ◽

Daily Intake ◽

Bacillus Coagulans ◽

The United States ◽

Nutrient Digestibility ◽

Intestinal Health ◽

Gross Energy

Abstract Functional pet foods, such as those containing probiotics, are considered a key growth driver in the $37 billion market of dog and cat foods in the United States. Of particular interest to pet food applications is the patented bacterial strain Bacillus coagulans (GBI-30, 6086), a commercially available spore-forming non-toxigenic microorganism with high resiliency to stresses associated with commercial manufacturing. The objectives of this research were to examine the effect of Bacillus coagulans on stool quality, nutrient digestibility, and intestinal health markers in healthy dogs. High-protein, grain-free extruded diets containing graded levels of probiotic applied through extrusion or as a topical coating were fed to ten adult Beagle dogs of similar age. The study was designed as 5 x 5 replicated Latin square with 16-d adaptation followed by 5-d total fecal collection for each period. The five dietary treatments were formulated to deliver a dose of 0-, 6-, 7-, 8-, and 9-log10 colony-forming units (CFU) per dog per day. Data were analyzed using a mixed model through SAS (version 9.3, SAS Institute, Inc., Cary, NC) with treatment and period as fixed effects and dog as random effect. Apparent digestibility of organic matter, crude protein, crude fat, and gross energy were greatest for dogs fed the 9-log10 dose treatment (85.8, 83.6, 91.7, 84.0%, respectively), with increases (P < 0.05) observed in gross energy and organic matter digestibility compared to the negative control. Crude protein digestibility tended (P < 0.10) to increase as probiotic dose increased. No significant differences were observed in food intake, stool quality, fecal pH, fecal ammonia, or fecal short chain fatty acids. These results suggest that Bacillus coagulans has a favorable impact on nutrient digestibility and no apparent adverse effects when added to extruded diets at a daily intake level of up to 9-log10 CFU in healthy adult dogs.

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Evaluation of graded levels of Bacillus coagulans GBI-30, 6086 on apparent nutrient digestibility, stool quality, and intestinal health indicators in healthy adult dogs

Journal of Animal Science ◽

10.1093/jas/skab137 ◽

2021 ◽

Author(s):

Heather L Acuff ◽

Charles G Aldrich

Keyword(s):

Fatty Acids ◽

Organic Matter ◽

Mixed Model ◽

Healthy Adult ◽

Daily Intake ◽

Bacillus Coagulans ◽

Nutrient Digestibility ◽

Intestinal Health ◽

Gross Energy ◽

Chain Fatty Acids

Abstract Bacillus coagulans GBI-30, 6086 is a commercially available spore-forming non-toxigenic microorganism approved for use in dog foods with high resiliency to stresses associated with commercial manufacturing. The objectives of this research were to examine the effect of Bacillus coagulans on stool quality, nutrient digestibility, and intestinal health markers in healthy adult dogs. Extruded diets containing graded levels of Bacillus coagulans applied either to the base ration before extrusion or to the exterior of the kibble as a topical coating after extrusion were randomly assigned to ten individually housed adult beagle dogs (7 castrated males, 3 spayed females) of similar age (5.75 ± 0.23 years) and body weight (12.3 ± 1.5 kg). The study was designed as 5 x 5 replicated Latin square with 16-d adaptation followed by 5-d total fecal collection for each period. Five dietary treatments were formulated to deliver a dose of 0-, 6-, 7-, 8-, and 9-log10 colony-forming units (CFU) per dog per day for the control (CON), extruded Bacillus coagulans (PEX), and low, moderate, and high Bacillus coagulans coating levels (PCL, PCM, and PCH), respectively. Food-grade TiO2 was added to all diets at a level of 0.4% to serve as an indigestible dietary marker for digestibility calculations. Data were analyzed using a mixed model through SAS (version 9.4, SAS Institute, Inc., Cary, NC) with treatment as a fixed effect and room (i.e., replicate), period, and dog(room) as random effects. Apparent total tract digestibility of organic matter, crude protein, crude fat, and gross energy calculated by the marker method were numerically greatest for dogs fed the 9-log10 dose treatment with increases (P < 0.05) observed in gross energy and organic matter digestibility compared to the negative control. No significant differences were observed in food intake, stool quality, fecal pH, fecal ammonia, fecal short-chain fatty acids, or branched-chain fatty acids for the extruded Bacillus coagulans treatment (PEX) or the coated Bacillus coagulans treatments (PCL, PCM, and PCH) compared to CON. These results suggest that Bacillus coagulans has a favorable impact on nutrient digestibility and no apparent adverse effects when added to extruded diets at a daily intake level of up to 9-log10 CFU in healthy adult dogs.

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A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

Microorganisms ◽

10.3390/microorganisms9071451 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1451

Author(s):

Alip Kumar ◽

Sarbast K. Kheravii ◽

Catherine Ionescu ◽

Alexandra Blanchard ◽

Reza Barekatain ◽

...

Keyword(s):

Goblet Cell ◽

Fluorescein Isothiocyanate ◽

Goblet Cells ◽

Cell Number ◽

Necrotic Enteritis ◽

Intestinal Health ◽

Intestinal Integrity ◽

Eimeria Spp ◽

Half Dose ◽

Fluorescein Isothiocyanate Dextran

This study was conducted to examine the effects of a plant extract mixture, a microencapsulated product composed of eugenol and garlic tincture (PE), on intestinal health in broilers under necrotic enteritis (NE) challenge. A total of 960 d-old mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens housing 20 birds per pen. Six treatments were applied: UC, unchallenged control; CC, challenged control; PE, challenged group plus PE; AM, challenged group plus antimicrobial (AM); FAP, challenged group plus a full dose of AM with PE; HAP, challenged group plus a half dose of AM with PE in starter, grower and finisher phases. Birds in the challenged groups were inoculated with Eimeria spp. on d 9 and Clostridium perfringens on d14. On d 16, the CC group had increased serum fluorescein isothiocyanate dextran (FITC-d), reduced villus surface area, goblet cell number, upregulated CLDN1, JAM2 genes and reduced microbial diversity compared to the UC group (p < 0.05). Birds fed PE had reduced FITC-d, increased goblet cell number and Bifidobacterium compared to the CC group (p < 0.05). Birds fed PE had reduced CLDN5 expression in male birds, and Bacteroides spp. in female birds than CC group (p < 0.05). These findings suggest that PE supplementation mitigates the effect of NE by improving the intestinal health of birds.

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Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽

10.3390/ani11072027 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2027

Author(s):

Doaa Ibrahim ◽

Tamer Ahmed Ismail ◽

Eman Khalifa ◽

Shaimaa A. Abd El-Kader ◽

Dalia Ibrahim Mohamed ◽

...

Keyword(s):

Growth Performance ◽

Clostridium Perfringens ◽

Broiler Chickens ◽

Body Weight Gain ◽

Economic Benefits ◽

Production Costs ◽

Broiler Chicks ◽

Necrotic Enteritis ◽

Feed Conversion ◽

Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

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The influence of xylanase on the fermentability, digestibility, and physiochemical properties of insoluble corn-based fiber along the gastrointestinal tract of growing pigs

Journal of Animal Science ◽

10.1093/jas/skab159 ◽

2021 ◽

Author(s):

Amy L Petry ◽

Nichole F Huntley ◽

Michael R Bedford ◽

John F Patience

Keyword(s):

Mixed Model ◽

Linear Mixed Model ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Neutral Detergent Fiber ◽

Adaptation Period ◽

High Fiber ◽

Gross Energy ◽

Digesta Viscosity ◽

The Impact

Abstract In theory, supplementing xylanase in corn-based swine diets should improve nutrient and energy digestibility and fiber fermentability, but its efficacy is inconsistent. The experimental objective was to investigate the impact of xylanase on energy and nutrient digestibility, digesta viscosity, and fermentation when pigs are fed a diet high in insoluble fiber (>20% neutral detergent fiber; NDF) and given a 46-d dietary adaptation period. Three replicates of 20 growing gilts were blocked by initial body weight, individually housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% NDF, a 30% corn bran high-fiber control (HF; 21.9% NDF), HF+100 mg xylanase/kg [HF+XY, (Econase XT 25P; AB Vista, Marlborough, UK)] providing 16,000 birch xylan units/kg; and HF+50 mg arabinoxylan-oligosaccharide (AXOS) product/kg [HF+AX, (XOS 35A; Shandong Longlive Biotechnology, Shandong, China)] providing AXOS with 3-7 degrees of polymerization. Gilts were allowed ad libitum access to fed for 36-d. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed, and feces were collected. On d 46, pigs were euthanized and ileal, cecal, and colonic digesta were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment as a fixed effect. Compared with LF, HF reduced the apparent ileal digestibility (AID), apparent cecal digestibility (ACED), apparent colonic digestibility (ACOD), and apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), crude protein (CP), acid detergent fiber (ADF), NDF, and hemicellulose (P<0.01). Relative to HF, HF+XY improved the AID of GE, CP, and NDF (P<0.05), and improved the ACED, ACOD, and ATTD of DM, GE, CP, NDF, ADF, and hemicellulose (P<0.05). Among treatments, pigs fed HF had increased hindgut DM disappearance (P=0.031). Relative to HF, HF+XY improved cecal disappearance of DM (162 vs. 98g; P=0.008) and NDF (44 vs. 13g; P<0.01). Pigs fed xylanase had a greater proportion of acetate in cecal digesta and butyrate in colonic digesta among treatments (P<0.05). Compared with LF, HF increased ileal, cecal, and colonic viscosity, but HF+XY decreased ileal viscosity compared with HF (P<0.001). In conclusion, increased insoluble corn-based fiber decreases digestibility, reduces cecal fermentation, and increases digesta viscosity, but supplementing xylanase partially mitigated that effect.

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Effects of Defatted Rice Bran Inclusion Level on Nutrient Digestibility and Growth Performance of Different Body Weight Pigs

Animals ◽

10.3390/ani11051374 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1374

Author(s):

Bingbing Huang ◽

Huangwei Shi ◽

Li Wang ◽

Lu Wang ◽

Zhiqian Lyu ◽

...

Keyword(s):

Body Weight ◽

Growth Performance ◽

Rice Bran ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Neutral Detergent Fiber ◽

Control Group ◽

Defatted Rice Bran ◽

Gross Energy ◽

Stage 1

This study was conducted to determine the effects of low-protein diet prepared with different levels of defatted rice bran (DFRB) and weight stages on growth performance and nutrient digestibility of growing–finishing pigs. The animal experiment included three stages. A total of 240 growing pigs with an initial body weight of 28.06 ± 8.56 kg for stage 1 were allocated to five diets including one control group and four DFRB diets supplemented with 2.5%, 5%, 7.5% and 10% DFRB, respectively. The 192 crossbred pigs with initial body weights of 55.03 ± 7.31 kg and 74.55 ± 9.10 kg were selected for stage 2 and stage 3, respectively. Pigs were allocated to four diets including one control group and three DFRB diets supplemented with 10%, 15% and 20% DFRB, respectively. The results showed that with the increase in DFEB intake, the gain: feed was linearly increased (p < 0.05), and the average daily feed intake tended to linearly decrease (p = 0.06) in stage 1. Except for the apparent total tract digestibility (ATTD) of acid detergent fiber (ADF) in stage 3, levels of DFRB had significant effects on the ATTD of gross energy (GE), dry matter (DM), ash, neutral detergent fiber (NDF) and ADF in three weight stages. In stage 1, with the increase in levels of DFRB, the ATTD of NDF and hemicellulose were firstly increased and then decreased (p < 0.01). In stage 2, with the increasing levels of DFRB, the ATTD of DM, ash and cellulose were firstly increased and then decreased (p < 0.01). In stage 3, the ATTD of GE, DM, ash, NDF and hemicellulose decreased linearly with the increase in levels of DFRB (p < 0.01). Collectively, DFRB could be used as a replacement for corns and soybean meal, and weight stage is important to consider when adjusting the additive proportion.

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Wheat bran fermented by mixed fungal strains improves the digestibility of crude fiber and may benefit the gut health without impacting the growth performance in weaned pigs

Food & Function ◽

10.1039/d1fo00273b ◽

2021 ◽

Vol 12 (7) ◽

pp. 2962-2971

Author(s):

Yuheng Luo ◽

Jun He ◽

Hua Li ◽

Cong Lan ◽

Jingyi Cai ◽

...

Keyword(s):

Growth Performance ◽

Wheat Bran ◽

Nutrient Digestibility ◽

Crude Fiber ◽

Gut Health ◽

Intestinal Health ◽

Weaned Piglets ◽

Weaned Pigs ◽

Fungal Strains

This study was conducted to compare the effect of raw (WB) or mixed fungi-fermented wheat bran (FWB) on the growth, nutrient digestibility and intestinal health in weaned piglets.

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Impact of four fiber-rich supplements on nutrient digestibility, colostrum production, and farrowing performance in sows

Journal of Animal Science ◽

10.1093/jas/skab247 ◽

2021 ◽

Vol 99 (9) ◽

Author(s):

Takele Feyera ◽

Liang Hu ◽

Maria Eskildsen ◽

Thomas S Bruun ◽

Peter K Theil

Keyword(s):

Dry Matter ◽

Palm Kernel ◽

Nutrient Digestibility ◽

Extra Energy ◽

Lower Blood ◽

Nonstarch Polysaccharides ◽

Gross Energy ◽

Soy Hulls ◽

Beet Pulp ◽

The Impact

Abstract This study aimed to investigate the impact of dietary fiber (DF) sources on sow and litter performance, and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrients. A total of 48 sows were stratified for body weight at mating and randomly assigned to one of four DF sources (mixed fiber [MF], palm kernel expellers [PKE], sugar beet pulp [SBP], or soy hulls [SH]) and fed the diet from mating until farrowing. Within DF treatments, sows were supplemented with one of two extra energy sources (glycerol or sugar dissolved in water), whereas a third group (control) received water from day 108 of gestation until farrowing. The number of total born, live-born, and stillborn pigs; birth time and birth weight of the pigs; farrowing duration; and farrowing assistance (FA) were recorded. Live-born pigs were weighed again at 12 and 24 h after birth to record weight gain, which was used to estimate intake and yield of colostrum. Blood samples were collected once daily from day −3 relative to farrowing until day 1 after farrowing in sows and once from selected pigs right after birth. Fecal samples were collected on day 114 of gestation and colostrum at 0, 12, 24, and 36 h after onset of farrowing. Intake of soluble and insoluble nonstarch polysaccharides (NSP) was greater for SBP (P < 0.001) and PKE (P < 0.001) supplemented sows, respectively, when compared with other groups. Farrowing duration and stillbirth rate were not affected by DF sources, but PKE and SH supplemented sows had greater FA than SBP and MF supplemented sows (P < 0.001). Extra energy supplement did not improve the farrowing performance. Concentration (P = 0.02) and output (P = 0.04) of dry matter in colostrum, and ATTD of GE (P < 0.001) and crude protein (CP; P < 0.001) were lower for PKE supplemented sows than in sows from the remaining groups. Intake of insoluble NSP correlated negatively with ATTD of GE (P < 0.001) and CP (P < 0.001). Concentrations of glucose (P < 0.001), lactate (P < 0.001), CO2 (P < 0.001), and HCO3 (P < 0.001) in sows blood were increased with time progress relative to farrowing. Newborn pigs from PKE supplemented sows had greater concentration of lactate (P = 0.02) and lower blood pH (P = 0.02) than the remaining treatments. In conclusion, PKE supplement reduced ATTD of GE and CP, and concentration and output of dry matter in colostrum but increased FA. Results of this experiment indicated that the use of PKE as a fiber source for late gestating sows should be avoided.

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Feed Utilisation Efficiency of Lupin Inclusion in Cobia: Role of Dietary Organic Selenium Supplementation

Modern Applied Science ◽

10.5539/mas.v10n10p180 ◽

2016 ◽

Vol 10 (10) ◽

pp. 180 ◽

Cited By ~ 2

Author(s):

Hung Duc Pham ◽

Ravi Fotedar ◽

Chau Minh Nguyen ◽

Muhammad Abu Bakar Siddik

Keyword(s):

Crude Protein ◽

Nutrient Digestibility ◽

Organic Selenium ◽

Gross Energy ◽

Feed Utilisation ◽

Kernel Meal ◽

Flow Through ◽

Inclusion Level ◽

Utilisation Efficiency

The dietary selenium (Se) requirement has been determined for cobia Rachycentron canadum using purified diet; however, its role in the utilisation of plant-derived ingredients has not been evaluated in the species. Therefore, a 3 x 2 factorial experimental design involving three inclusion levels (0, 210 and 315 g/kg) of lupin Lupinus angustifolius kernel meal (LKM) and two concentrations (0 and 0.8 mg/kg) of Se was used to evaluate the effects of Se supplementation in conjunction with LKM on the growth, feed utilisation and physiological responses in the cobia. Six isonitrogenous (46.5 % crude protein) and isoenergetic (21 MJ/kg gross energy) diets were formulated and fed to cobia for 7 weeks in a flow-through seawater system. The results showed significant effects of Se supplementation and its interaction with dietary lupin on the growth and feed efficiency of cobia. Se supplementation significantly improved the growth and feed utilisation efficiency in cobia fed lupin-based diets. The nutrient digestibility of fish fed supplemental Se lupin-based diets was significantly higher than that of fish fed diets without Se supplementation at each inclusion level of LKM. There were no significant effects of dietary Se supplementation on the survival, muscle composition or muscle amino acids, whereas regression analysis indicated a positive linear relationship between tissue Se accumulation and dietary Se levels. An enhancement of haematological responses was also observed in cobia fed Se-supplemented diets. In summary, cobia fed lupin-based diets required higher dietary Se supplementation for higher feed utilisation efficiency than previously quantified for the casein-based diet.

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