scholarly journals Microbiological Safety and Presence of Major Mycotoxins in Animal Feed for Laboratory Animals in a Developing Country: The Case of Costa Rica

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2389
Author(s):  
Fabio Granados-Chinchilla ◽  
Carol Valenzuela-Martínez ◽  
Berny García-Murillo ◽  
David Aguilar-Madrigal ◽  
Mauricio Redondo-Solano ◽  
...  
Keyword(s):  
Costa Rica ◽  
Animal Feed ◽  
Uv Light ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Laboratory Animals ◽  
Salmonella Spp ◽  
Indicator Microorganisms ◽  
Laboratory Rodents ◽  
D Values

Safety and quality of compound feed for experimental animals in Costa Rica is unknown. Some contaminants, such as Salmonella spp. and mycotoxins, could elicit confounding effects in laboratory animals used for biomedical research. In this study, different batches of extruded animal feed, intended for laboratory rodents in Costa Rica, were analyzed to determine mycotoxin and microbiological contamination (i.e., Salmonella spp., Escherichia coli, total coliform bacteria, and total yeast and molds enumeration). Two methods for Salmonella decontamination (UV light and thermal treatment) were assessed. Only n = 2 of the samples were negative (representing 12.50%) for the 26 mycotoxins tested. Enniatins and fumonisins were among the most frequent toxins found (with n = 4+ hits), but the level of contamination and the type of mycotoxins depended on the supplier. None of the indicator microorganisms, nor Salmonella, were found in any of the tested batches, and no mold contamination, nor Salmonella growth, occurs during storage (i.e., 2–6 months under laboratory conditions). However, mycotoxins, such as enniatins and fumonisins tend to decrease after the fourth month of storage, and Salmonella exhibited a lifespan of 64 days at 17 °C even in the presence of UV light. The D-values for Salmonella were between 65.58 ± 2.95 (65 °C) and 6.21 ± 0.11 (80 °C) min, and the thermal destruction time (z-value) was calculated at 15.62 °C. Results from this study suggest that laboratory rodents may be at risk of contamination from animal feed that could significantly affect the outcomes of biomedical experiments. Thus, improved quality controls and handling protocols for the product are suggested.

scholarly journals An assessment of the potability of groundwater from boreholes based on microbial quality and physical properties: case of Buea and Tiko Subdivisions, South West Region of Cameroon

2021 ◽  
Vol 16 (2) ◽  
pp. 147-163
Author(s):  
Helen Epouse Azise Ayimele ◽  
Melisa Bijingisi ◽  
Samuel Ayonghe
Keyword(s):  
Physical Properties ◽  
Total Coliform ◽  
Physical Characteristics ◽  
Coliform Bacteria ◽  
Physical Parameters ◽  
Microbial Quality ◽  
Salmonella Spp ◽  
E Coli ◽  
Two Samples ◽  
Eaux Souterraines

Groundwater from boreholes, though generally considered as potable water, is vulnerable to both microbial pollution and unsuitable physical characteristics. An assessment of the microbial quality and the physical properties of groundwater from boreholes was accordingly carried out in Buea and Tiko subdivisions to determine these parameters. In particular, the presence of coliform bacteria and an eventual isolation of E. coli and Salmonella spp which are faecal indicator organisms were carried out. A total of 46 samples were collected from 29 randomly selected boreholes and their physical parameters (temperature, pH, EC, TDS and turbidity), and microbial content analyzed. Physical parameters were measured in-situ using portable field devices while total coliform count and isolation of faecal coliforms (E. coli and Salmonella spp) were determined using standard microbiological methods with reagents, Violet Red Bile Agar, Mackonkey Agar and Salmonella Shigella Agar respectively. The results indicated that the physical characteristics of the water (pH range 4.9-8.4; EC range 90µS/cm-233µS/cm; TDS range 61 mg/L-1630 mg/L; Turbidity range 0.44NTU-59.80NTU) were within WHO guidelines for potability except for the turbidity with barely two samples that fell within the acceptable range of 1.5NTU. Total coliform counts ranged from 1x100CFU/ml to 3x103CFU/ml and 72.41% of the samples exceeded the WHO recommended 0.0 CFU/100 ml. E. coli was isolated in 76.47 % while Salmonella spp was isolated in 82.35% of the samples. This pioneer study on groundwater quality from boreholes in the study area reveals that the water is vulnerable to bacteriological pollution and therefore not potable. This necessitates awareness creation of the community on the regular disinfection of groundwater from boreholes. RESUMELes eaux souterraines des forages, bien que généralement considérées comme de l’eau potable, sont vulnérables à la fois à la pollution microbienne et à des caractéristiques physiques inappropriées. Une évaluation de la qualité microbienne et des propriétés physiques des eaux souterraines des forages a donc été réalisée dans les départements de Buea et Tiko pour déterminer ces paramètres. En particulier, la présence de bactéries coliformes et un éventuel isolement d’E. Coli et l’espèce de Salmonella qui sont des organismes indicateurs de contamination fécale ont été réalisés. Au total, 46 échantillons ont été prélevés dans 29 forages sélectionnés au hasard et leurs paramètres physiques (température, pH, CE, TDS et turbidité) et leur contenu microbien ont été analysés. Les paramètres physiques ont été mesurés sur place à l’aide d’appareils de terrain portables, tandis que le nombre total de coliformes et l’isolement des coliformes fécaux (E. coli et Salmonella spp) ont été déterminés à l’aide de méthodes microbiologiques standard avec des réactifs, la gélose violette rouge, la gélose Mackonkey et la gélose Salmonella Shigella respectivement. Les résultats ont indiqué que les caractéristiques physiques de l’eau (plage de pH 4,9- 8,4; plage CE 90µS/cm-2330µS/cm; plage TDS 61 mg/L-1630 mg/L; plage de turbidité 0,44NTU- 59.80NTU) étaient conformes au directives de l’OMS pour la potabilité. Seul, la turbidité avait à peine deux échantillons qui se situaient dans la plage acceptable de 1,5NTU. Le nombre total de coliformes variait de 1x100 CFU/ml à 3x103 CFU/ml et 72,41% des échantillons dépassaient les 0,0 CFU/100 ml recommandés par l’OMS. E. coli a été isolé dans 76,47% tandis que l’espèce Salmonella a été isolée dans 82,35% des échantillons. Cette étude pionnière sur la qualité des eaux souterraines des forages dans la zone d’étude révèle que l’eau est vulnérable à la pollution bactériologique et donc non potable. Cela nécessite une prise de conscience de la communauté sur la désinfection régulière des eaux souterraines des forages.

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

Bacterial die-away rates in red sea waters

1995 ◽  
Vol 32 (2) ◽  
pp. 45-52 ◽  
Author(s):  
H. Z. Sarikaya ◽  
A. M. Saatçi
Keyword(s):  
Solar Radiation ◽  
Decay Rate ◽  
Sea Water ◽  
Membrane Filter ◽  
Linear Regression Analysis ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Decay Rate Constant ◽  
Solar Intensity ◽  
Log Normal

Total coliform bacteria have been chosen as the indicator organism. Coliform die-away experiments have been carried out in unpolluted sea water samples collected at about 100 m off the coastline and under controlled environmental conditions. The samples were transformed into one litre clean glass beakers which were kept at constant temperature and were exposed to the solar radiation. The membrane filter technique was used for the coliform analysis. The temperature ranged from 20 to 40° C and the dilution ratios ranged from 1/50 to 1/200. Coliform decay rate in the light has been expressed as the summation of the coliform decay rate in the dark and the decay rate due to solar radiation. The solar radiation required for 90 percent coliform removal has been found to range from 17 cal/cm2 to 40 cal/cm2 within the temperature range of 25 to 30° C. Applying the linear regression analysis two different equations have been given for the high (I>10 cal/cm2.hour) and low solar intensity ranges in order to determine the coliform decay rate constant as a function of the solar intensity. T-90 values in the light have been found to follow log-normal distribution with a median T-90 value of 32 minutes. The corresponding T-90 values in the dark were found to be 70-80 times longer. Coliform decay rate in the dark has been correlated with the temperature.

H2S papers as presumptive tests for Salmonella contamination in tropical drinking water

1996 ◽  
Vol 34 (7-8) ◽  
pp. 187-194 ◽  
Author(s):  
Tanya Gawthorne ◽  
Robyn A. Gibbs ◽  
Kuruvilla Mathew ◽  
Goen E. Ho
Keyword(s):  
Drinking Water ◽  
Hydrogen Sulphide ◽  
Incubation Temperature ◽  
Indicator Bacteria ◽  
Coliform Bacteria ◽  
Salmonella Spp ◽  
Faecal Contamination ◽  
Tropical Waters ◽  
Remote Areas ◽  
Traditional Indicator

Coliform bacteria may not be adequate as sole indicators of recent faecal contamination in tropical waters. Salmonella spp. in particular have been found in tropical waters in the absence of traditional indicator bacteria. Remote areas without access to a laboratory have no opportunity for salmonellae analysis as portable tests are currently not available. H2S papers indicate the presence of hydrogen sulphide producing bacteria, a characteristic shared by the majority of Salmonella spp.. The potential of H2S papers to act as a presumptive test for salmonellae in the absence of coliform bacteria was assessed. Salmonella spp. grew in the H2S medium, with an optimum incubation temperature of 37°C and a recommended length of incubation for a negative result of 48 hours. The presence of high numbers of the type of noncoliform bacteria commonly found in drinking water did not affect the performance of the H2S strips. H2S papers are recommended for use in conjunction with a coliform test as a presumptive test for the presence of Salmonella spp. in drinking water.

scholarly journals Improvement of waste water quality by application of mixed algal inocula

2018 ◽  
Vol 53 (1) ◽  
pp. 77-82
Author(s):  
MA Riaz ◽  
B Ijaz ◽  
A Riaz ◽  
M Amjad
Keyword(s):  
Waste Water ◽  
Experimental Treatment ◽  
Total Coliform ◽  
Post Treatment ◽  
Coliform Bacteria ◽  
Physiochemical Parameters ◽  
Pollutant Concentrations ◽  
Pre Treatment ◽  
Wastewater Samples ◽  
The One

Present study aim to investigate application of microalgae for improvement of physiochemical parameters of waste water and improvement of treatment process through reducing the pollutant concentrations including coliform bacteria through their ability to uptake nutrients. Aquatic cultures were conducted in container with dimensions of 1.5×3sq ft. and wastewater samples 5 Litre samples were collected in plastic cans from Sukhnar drain and algal samples were collected from Bhaini Sigyain Village near Ravi Siphon, Lahore. The one experimental treatment lasted for 10 days to make 50% 5 liter water added concentration and 200g of inocula of algae were grown in wastewater container for experiments to optimize at room temperature as local outdoor cultures conditions for wastewater treatment. Total experiment repeated thrice and completed in 30 days. Results show that treatments improved the physiochemical parameter after week. Turbidity (NTU), total suspended solids (mg/L), total dissolved solids (mg/L), nitrate (mg/L), phosphate (mg/L) decrease (P < 0.05) after treatment. However, pH not significantly decreases (P≥ 0.05) pre-treatment values 6.56±0.05 (mg/L) and post-treatment 6.53± 0.057(mg/L). Maximum total coliform disinfection (P < 0.05) was indicated with 200 g of algae was pre-treatment 1602± 6.8 (MPN) and post treatment 1447±39.5 (MPN) observed.Bangladesh J. Sci. Ind. Res.53(1), 77-82, 2018

scholarly journals Use of ceramic pot filter (CPF) technology under pressure in an in-line pumping system

2017 ◽  
Vol 18 (3) ◽  
pp. 843-852
Author(s):  
Travis D. Gardner ◽  
Joe D. Guggenberger
Keyword(s):  
Water Treatment ◽  
Low Cost ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
Limiting Factor ◽  
Water Production ◽  
Pumping System ◽  
Point Of Use ◽  
Logarithmic Reduction

Abstract Ceramic pot filters (CPFs) are an effective point of use water treatment device in developing nations due to their low cost and effectiveness. CPFs are gravity fed, typically making water production a major limiting factor to a CPF's lifetime and acceptability. Directly connecting CPFs to in-line pumping systems or systems with an elevated storage tank would allow filter usage for constant water treatment at increased pressures, increasing the quantity of treated water. Ceramic disks were manufactured for testing in a specially designed housing apparatus. Filters of varying thicknesses and clay to sawdust mass ratios were manufactured to fit tightly. Flowrate and microbiological removal efficacy (logarithmic reduction value (LRV)) were determined over the testing period at various pressures. Flowrate values ranged from 2.44 to 9.04 L per hour, significantly higher than traditional CPF technology. LRVs ranged from 1.1 to 2.0, lower than traditional CPF technology but still effective at removing most Escherichia coli and total coliform bacteria. Filters proved effective at removing total and fecal coliforms at pressures less than 70 kilopascals. The optimum filter had a thickness of 3.2 cm and clay to sawdust ratio of 6:1 by mass. Filters proved to be ineffective if flowrates were above 5 L/h.

scholarly journals Microbiological evaluation of lettuce produced by conventional and organic systems in farms of Londrina, PR

2017 ◽  
Vol 38 (1) ◽  
pp. 175 ◽  
Author(s):  
Natalia Harumi Niguma ◽  
Jacinta Sanchez Pelayo ◽  
Tereza Cristina Rocha Moreira de Oliveira
Keyword(s):  
Irrigation Water ◽  
Water Samples ◽  
Total Coliform ◽  
Organic Production ◽  
Conventional System ◽  
Salmonella Spp ◽  
Organic System ◽  
E Coli ◽  
Organic Systems ◽  
Production Practices

The aims of this study were to evaluate the contamination of lettuce (Lactuca sativa), produced in Londrina, Paraná (PR), with total coliform, coliform at 45 °C, E. coli, and Salmonella spp.; and to determine the E. coli contamination of irrigation water used at the farms studied. Four farms were evaluated, of which three produced lettuce using a conventional system and one using an organic system. An evaluation of the production practices of the farms was also carried out. A total of 111 samples were analyzed, 71 lettuce samples from the conventional system and 40 samples from the organic system. A total of eight irrigation water samples were collected for analysis. Coliform at 45 °C counts above the limit tolerated by Brazilian legislation were observed in 2.8% (2/71) of conventionally grown lettuce samples, and Salmonella spp. was isolated in 1.4% (1/71) of those samples. In the organic lettuce samples, 12.5% (5/40) had coliform at 45 °C counts above the limit tolerated and Salmonella spp. was not detected. Irrigation water samples from three farms were unsatisfactory, with counts higher than 102MPN of E. coli per 100mL. The results of this study demonstrate that most conventionally grown lettuce samples show good sanitary conditions in production, and that lettuce contamination is not related to contamination found in irrigation water samples. The results also showed that the organic production practices required by Brazilian certification agencies should be applied to ensure that contamination of produced lettuce remains controlled.

scholarly journals Tokat Bez Sucuğunun Mikrobiyolojik Kalitesinin İncelenmesi

2020 ◽  
Vol 8 (12) ◽  
pp. 2683-2694
Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Microbiological Parameters

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform

scholarly journals Antibiogram Profiling and Thermal Inactivation of Staphylococcus aureus and Escherichia coli Isolated from Milk of Dharan, Nepal

2020 ◽  
pp. 81-87
Author(s):  
Susmita Phattepuri ◽  
Prince Subba ◽  
Arjun Ghimire ◽  
Shiv Nandan Sah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Thermal Inactivation ◽  
Total Coliform ◽  
Plate Count ◽  
Diffusion Method ◽  
E Coli ◽  
Coliform Count ◽  
Z Value ◽  
D Values

Milk is an excellent medium for the growth of many bacteria. This study aimed to determine antibiotic profiling and thermal inactivation of Staphylococcus aureus and Escherichia coli isolated from raw milk of Dharan. Total viable count, total Staphylococcal count, and total coliform count were carried out by conventional microbiological methods. Identification was done on the basis of Gram staining and biochemical tests. The antibiotic susceptibility test of the isolates carried out by the modified Kirby-Baur disc diffusion method. Thermal inactivation of S. aureus and E. coli were carried out by subjecting to thermal treatment in a water bath. Total plate count ranged from 204×104 CFU/mL to 332×105 CFU/mL. Total staphylococcal count and total coliform count ranged from 14×105 CFU/mL to 8×106 CFU/mL and 11×104 CFU/mL to 3×106 CFU/mL respectively. S. aureus showed an increasing resistance patterns towards Ampicillin, Cefotixin, Carbenicillin and Cefotaxime. Ciprofloxacin, Erythromycin, Amikacin, Gentamycin, Azithromycin, and Chloramphenicol were found to be effective against S. aureus. All the E. coli isolates were resistant to Ampicillin and least resistant to Cefotixin. Chloramphenicol, Amikacin, Azithromycin, and Nalidixic acid were found highly effective to E. coli. The D-values for S. aureus at 56°C, 58°C and 60°C were 1.36 min, 1.19 min, and 1.09 min respectively. The Z-value was 14.92°C. While D-values were obtained as 0.98 min, 0.75 min, and 0.57 min for E. coli at 56° C, 58° C and 60° C respectively, and Z-value was 9.75° C. Hence, S. aureus was found to be more heat resistant than E. coli.

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