Pharmacological Potential and Chemical Characterization of Bridelia ferruginea Benth.—A Native Tropical African Medicinal Plant

To avail the possible pharmacological actions of Brideliaferruginea Benth., the present investigation was designed to quantitatively analyze the total flavonoid and phenolic contents and assess the various antioxidant and enzyme inhibition properties of leaf and stem bark extracts (ethyl acetate, water and methanolic) of B. ferruginea. Anti-proliferative effect was also investigated against human colon cancer cells (HCT116) as well as the antimicrobial potential against multiple bacterial and fungal (yeasts and dermatophytes) strains. The methanolic and water extracts of the stem bark demonstrated the highest phenolic content (193.58 ± 0.98 and 187.84 ± 1.88 mg/g, respectively), while the leaf extracts showed comparatively higher flavonoid contents (24.37–42.31 mg/g). Overall, the methanolic extracts were found to possess the most significant antioxidant potency. Compared to the other extracts, methanolic extracts of the B. ferruginea were revealed to be most potent inhibitors of acetyl- and butyryl-cholinesterases, tyrosinase α-amylase, except α-glucosidase. Only the ethyl acetate extracts were found to inhibit glucosidase. Additionally, the stem bark methanolic extract also showed potent inhibitory activity against E. coli and gram-positive bacteria (MIC (minimum inhibitory concentration): 2.48–62.99 µg/mL), as well as all the tested fungi (MIC: 4.96–62.99 µg/mL). In conclusion, B. ferruginea can be regarded as a promising source of bioactive compounds displaying multifunctional pharmacological activities and thus is a potential candidate for further investigations in the endeavor to develop botanical formulations for pharmaceutical and cosmeceutical industries.

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Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/1565306 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Luis M. Guamán-Ortiz ◽

Juan C. Romero-Benavides ◽

Alirica I. Suarez ◽

Stephania Torres-Aguilar ◽

Paola Castillo-Veintimilla ◽

...

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Human Colon ◽

Stem Bark ◽

Asiatic Acid ◽

Endemic Plant ◽

Human Colon Cancer ◽

Carcinoma Cell Lines ◽

The Status ◽

Cancer Types

Traditional herbal medicine has become an important alternative in the treatment of various cancer types, including colon cancer, which represents one of the main health problems around the world. Therefore, the search for new therapies to counteract this disease is very active. Grias neuberthii is an endemic plant located in the Ecuadorian Amazon region, which has been used in traditional medicine for its pharmacological properties, including its ability to inhibit tumor cell growth, although scientific studies are limited. We have analyzed the effect of this plant on two colon carcinoma cell lines, that is, RKO (normal p53) and SW613-B3 (mutated p53) cells. Among several extracts obtained from various parts of G. neuberthii plant, we identified the extract with the greatest cytotoxic potential, derived from the stem bark. The cytotoxic effect was similar on both cell lines, thus indicating that it is independent of the status of p53. However, significant differences were observed after the analysis of colony formation, with RKO cells being more sensitive than SW613-B3. No evidence for apoptotic markers was recorded; nevertheless, both cell lines showed signs of autophagy after the treatment, including increased Beclin-1 and LC3-II and decreased p62. Finally, three chemical compounds, possibly responsible for the effect observed in both cell lines, were identified: lupeol (1), 3′-O-methyl ellagic acid 4-O-β-D-rhamnopyranoside (2), and 19-α-hydroxy-asiatic acid monoglucoside (3).

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Phytochemical and Antifungal Efficacy of Leaf and Stem Bark Extracts of Parkia biglobosa on Fungi Associated with Seed Rot of Green Bean (Phaseolus vulgaries L.) in Akpabuyo, Cross River State, Nigeria

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.3.27 ◽

2016 ◽

Vol 3 ◽

pp. 27-38

Author(s):

Patrick Ishoro Akwaji ◽

Ekeng Ita Okon ◽

Etim Johnson Umana ◽

Aniedi Abasi Akpan Markson

Keyword(s):

Radial Growth ◽

Fungal Pathogens ◽

Cardiac Glycosides ◽

Stem Bark ◽

Leaf Extracts ◽

Cross River ◽

Cross River State ◽

Methanolic Extracts ◽

Antifungal Efficacy

The phytochemical and antifungal efficacy of the ethanolic and methanolic stem bark and leaf extracts of Parkiabiglobosa on the fungal pathogens isolated from infected green bean seeds obtained from the field in Akpabuyo, Cross River State, Nigeria were investigated in vitro. The pathogens were Botryodiplodiatheobromae and Aspergillusniger. Various concentrations of the extracts ranging from 10g/100ml, 20g/100ml, 30g/100ml, 40g/100ml and 50g/100ml were separately added to PDA media. Results of the in vitro antifungal assay shows that at 40g/100ml and 50g/100ml concentrations, ethanolic stem bark and leaf extracts of P. biglobosa completely inhibited the radial growth of B. theobromae and A. niger after seven days observation period while those of methanolic extracts completely inhibited the redial growth of the fungal pathogens at 50g/100ml concentration. Results, however, shows that the extracts had a significant effect (P<0.05) on the radial growth of the fungal pathogens at all the different concentrations tested as compared with the control. Pathogen growth inhibition was most effective at 30g/100ml, 40g/100ml and 50g/100ml concentrations. The inhibitory action of the extracts increased/decreased with a corresponding increase/decrease in the concentration of the plant extracts. Ethanolic stem bark and leaf extracts of P. biglobosa was found to be more effective in inhibiting the redial growth of the fungal pathogens than those of methanolic extracts, also ethanolic and methanolic stem bark extracts were observed to be more effective in the inhibition of the radial growth of the fungal pathogens than leaf extracts. Phytochemical screening of the extracts revealed the presence of cardiac glycosides, reducing sugars, flavonoids, saponnins and tannins with traces of alkaloids in stem bark extracts while flavonoids, alkaloids, cardiac glycosides, saponnins, tannins and terpenoids were present in leaf extracts.

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Morphomolecular identification, metabolic profile, anticancer, and antioxidant capacities of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 isolated from Egyptian remote cave

10.21203/rs.3.rs-438364/v1 ◽

2021 ◽

Author(s):

Waill A. Elkhateeb ◽

Walaa S. A. Mettwally ◽

Shireen A. A. Saleh ◽

Walid Fayad ◽

Ibrahim M. Nafady ◽

...

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Ethyl Acetate ◽

Human Breast Cancer ◽

Mcf7 Cell ◽

Human Colon ◽

Human Breast ◽

Human Colon Cancer ◽

Breast Cancer Mcf7 Cell

Abstract Searching for remote locations to screen for microorganisms, identify their metabolites, and investigate their bioactivities against lethal diseases such as cancer is of critical importance. In the current study, two fungal strains where isolated from a remote cave in Asyut governorate, Egypt. These isolates were morphologically and molecularly identified through sequencing their ITS region as Penicillium sp. NRC F1, and Penicillium sp. NRC F16. Investigating the metabolic profiles of the silylated ethyl acetate extracts of these fungi through conducting GC-Ms analysis revealed presence of 114 compounds belonging to different chemical classes. On the other hand, studying the in vitro bioactivity of both extracts showed moderate antioxidant activities. Penicillium sp. NRC F1 extract exhibited higher DPPH scavenging activity (74.41 ± 0.59%) at concentration of 200 µg/ml, in comparison with that exerted by Penicillium sp. NRC F16 extract at the same concentration (65.58 ± 1.55%). Moreover, studying the cytotoxicity of extracts against human colon cancer (HCT116), and human breast cancer (MCF7) cell lines revealed that cytotoxicity of both extracts was dose dependent. Promising cytotoxic effect was achieved against human colon cancer HCT116 using 200 µg/ml of Penicillium sp. NRC F1 extract (95.72 ± 1.13 % cytotoxicity), while Penicillium sp. NRC F16 ethyl acetate extract caused a cytotoxicity of 95.43 ± 1.4 %. Similarly, investigating the in vitro cytotoxicity of the extracts against human breast cancer MCF7 cell line resulted in observing promising activity of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 extracts, and they exhibited 97.29 ± 0.61 %; and 97.08 ± 1.07 % cytotoxicity, respectively. Results from this study nominate those strains as promising isolates and encourage for conducting further in vivo investigations to evaluate their potency.

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Pigments, Polyphenols and Antioxidant Activity of Leaf Extracts from Four Wild Rose Species Grown in Sicily

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha46211061 ◽

2018 ◽

Vol 46 (2) ◽

pp. 402-409 ◽

Cited By ~ 6

Author(s):

Francesca D'ANGIOLILLO ◽

Michele Massimo MAMMANO ◽

Giancarlo FASCELLA

Keyword(s):

Antioxidant Activity ◽

Morphological Characteristics ◽

Natural Antioxidants ◽

Total Flavonoids ◽

Leaf Extracts ◽

Methanolic Extracts ◽

Dpph Method ◽

Total Anthocyanins ◽

Total Flavonoids Content ◽

Promising Source

Leaf methanolic extracts of four wild Rose species (Rosa canina L., R. corymbifera Borkh., R. micrantha Borrer ex Sm and R. sempervirens L.) grown in Sicily were analysed in order to evaluate differences among indigenous roses which could be used such as source of bioactive compounds and natural antioxidants. Leaves were harvested during two different periods (June and October) and their morphological characteristics were described. Leaf content of chlorophylls, total anthocyanins, total flavonoids, total polyphenols (Folin-Ciocalteu method) and antioxidant activity (DPPH method) were determined. Results showed that the contents of chlorophylls and carotenoids were higher in leaves of all species harvested in June than in those harvested in October. On the contrary, total anthocyanins of R. micrantha and R. corymbifera leaves were higher in October. Total polyphenol content was higher in leaves of all species were harvested in October. No difference in total flavonoids content was recorded between the two harvest periods but only among the four species. Antioxidant activity was generally high in leaf extracts of all species in both periods and, particularly, in R. micrantha leaves harvested in June. These outcomes seem to support the assessment that leaves harvested from some Sicilian wild roses may be used as promising source of healthy compounds and that their content varies with the species and harvest period.

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Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts

Brazilian Journal of Biology ◽

10.1590/1519-6984.245753 ◽

2023 ◽

Vol 83 ◽

Author(s):

A. G. Toledo ◽

J. G. de L. de Souza ◽

C. B. Santana ◽

A. P. Mallmann ◽

C. V. dos Santos ◽

...

Keyword(s):

Methanol Extract ◽

Bacterial Strains ◽

Leaf Extracts ◽

Methanolic Extracts ◽

Biological Potential ◽

Sequestration Potential ◽

Ic50 Value ◽

Organic Extracts ◽

Phytochemical Profile ◽

Promising Source

Abstract The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

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The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer

Cancers ◽

10.3390/cancers12071913 ◽

2020 ◽

Vol 12 (7) ◽

pp. 1913

Author(s):

Noëlle Dommann ◽

Daniel Sánchez-Taltavull ◽

Linda Eggs ◽

Fabienne Birrer ◽

Tess Brodie ◽

...

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Human Colon ◽

Tumor Burden ◽

The Cancer Genome Atlas ◽

Cancer Tissue ◽

Potential Candidate ◽

Sequencing Data ◽

Human Colon Cancer ◽

Cancer Genome Atlas

Colorectal cancer, along with its high potential for recurrence and metastasis, is a major health burden. Uncovering proteins and pathways required for tumor cell growth is necessary for the development of novel targeted therapies. Ajuba is a member of the LIM domain family of proteins whose expression is positively associated with numerous cancers. Our data shows that Ajuba is highly expressed in human colon cancer tissue and cell lines. Publicly available data from The Cancer Genome Atlas shows a negative correlation between survival and Ajuba expression in patients with colon cancer. To investigate its function, we transduced SW480 human colon cancer cells, with lentiviral constructs to knockdown or overexpress Ajuba protein. The transcriptome of the modified cell lines was analyzed by RNA sequencing. Among the pathways enriched in the differentially expressed genes, were cell proliferation, migration and differentiation. We confirmed our sequencing data with biological assays; cells depleted of Ajuba were less proliferative, more sensitive to irradiation, migrated less and were less efficient in colony formation. In addition, loss of Ajuba expression decreased the tumor burden in a murine model of colorectal metastasis to the liver. Taken together, our data supports that Ajuba promotes colon cancer growth, migration and metastasis and therefore is a potential candidate for targeted therapy.

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ARGINASE INHIBITORY ACTIVITY OF STEM BARK EXTRACTS OF CAESALPINIA TORTUOSA ROXB

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.27 ◽

2018 ◽

Vol 10 (1) ◽

pp. 130

Author(s):

Amirah Najid ◽

Berna Elya ◽

Arikadia Noviani

Keyword(s):

Ethyl Acetate ◽

Inhibitory Activity ◽

Phenolic Content ◽

Colorimetric Method ◽

Stem Bark ◽

Phytochemical Analysis ◽

Standard Drug ◽

Methanol Extracts ◽

Methanolic Extracts ◽

Ic50 Values

Objective: This study aimed to evaluate the arginase inhibitory activity of Caesalpinia tortuosa Roxb. stem bark extracts.Methods: C. tortuosa Roxb. stem bark extracts were obtained through reflux extraction using n-hexane, ethyl acetate, and methanol and theirinhibitory activity against arginase was measured using a microplate reader at 430 nm. Active extracts were subjected to phytochemical analysisand based on the qualitative phytochemical analysis, quantitative data regarding flavonoid and phenolic contents were obtained. The total flavonoidcontent of active extracts was determined using AlCl3 colorimetric method, and the phenolic content was determined using Folin–Ciocalteu method.Results: Ethyl acetate and methanol extracts of C. tortuosa Roxb. inhibited activity of arginase with IC50 values of 33.81 and 11.58 μg/mL,respectively, nor-NOHA acetate as standard drug inhibited arginase with IC50 values of I3.77 μg/mL. Both active extracts contained saponins,tannins, and flavonoids. Ethyl acetate and methanol extracts showed a total flavonoid content of 7.41 mgQE/g and 5.05 mgQE/g and totalphenolic content of 27.55 mgGE/g and 17.16 mgGE/g, respectively. Methanol extracts had a higher inhibitory activity than ethyl acetateextracts despite having flavonoid and phenolic content, thereby suggesting no correlation between arginase inhibitory activity and flavonoidor phenolic content.Conclusion: Ethyl acetate and methanolic extracts of C. tortuosa Roxb. stem barks containing flavonoids, tannins, and saponins displayed arginaseinhibitory activity, and no correlation was observed between arginase inhibitory activity and flavonoid and phenolic content.

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Effects of stem bark and leaf extracts of Bridelia ferruginea on rat bladder smooth muscle

Fitoterapia ◽

10.1016/s0367-326x(00)00306-3 ◽

2001 ◽

Vol 72 (3) ◽

pp. 230-235 ◽

Cited By ~ 3

Author(s):

O Onoruvwe ◽

A.O Olayinka ◽

T.Y Lot ◽

F.V Udoh

Keyword(s):

Smooth Muscle ◽

Stem Bark ◽

Bladder Smooth Muscle ◽

Leaf Extracts ◽

Bridelia Ferruginea ◽

Rat Bladder

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CYTOTOXIC EFFECT OF CRUDE EXTRACT AND FRACTION FROM CALOTROPIS GIGANTEA LEAVES ON HUMAN COLON CANCER WIDR CELL LINES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i1.9022 ◽

2016 ◽

Vol 9 (1) ◽

pp. 83 ◽

Cited By ~ 1

Author(s):

Roihatul Mutiah ◽

Sukardiman . ◽

Aty Widyawaruyanti

Keyword(s):

Colon Cancer ◽

Ethyl Acetate ◽

Crude Extract ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Human Colon ◽

Human Colon Cancer ◽

Cytotoxic Effects ◽

Water Fraction ◽

Widr Cell

Objectives: This paper sought to understand and determine the cytotoxic’s effects of crude extract and its fraction from Calotropis gigantea leaves on human colon cancer WiDr cell lines.Methods: The ethanolic extract was fractionated gradually with certain substances to yield four fractions. The substances were dichloromethane, ethyl acetate, and butanol. The four fractions resulted in dichloromethane fraction, ethyl acetate fraction, butanol fraction, and a water fraction. These fractions were then investigated for their cytotoxic effects on WiDr cells. The cell viability was assessed using MTT colorimetric assay.Results: The result indicated that the cytotoxic effects of the ethanolic extract (IC5048.5 μg/ml), ethyl acetate fraction (IC5041.79 μg/ml), and dichloromethane fraction (IC5040.57μg/ml) produced a much more potent effect than the butanol fraction (IC50 737.74 μg/ml) and water fraction (IC508493 μg/ml).Conclusion: The ethanolic extract, ethyl acetate fraction and dichloromethane fraction exhibited a potent cytotoxic effect on human colon cancer WiDr cell line. The crude extract and fractions are potential to be developed as an anticancer agent in colon cancer therapy.

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In vitro anticancer potential and GC-MS analysis of Drimia nagarjunae, an endangered medicinal plant

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i2.21909 ◽

2015 ◽

Vol 10 (2) ◽

pp. 303 ◽

Cited By ~ 1

Author(s):

Narendranath Alluri ◽

Mala Majumdar

Keyword(s):

Ethyl Acetate ◽

Anticancer Activity ◽

Human Colon ◽

Human Colon Cancer ◽

Ms Analysis ◽

Endangered Medicinal Plant ◽

Colon Cancer Cell Lines ◽

Total Growth ◽

First Time

The aim of the present study was to investigate the anticancer activity of Drimia nagarjunae (Liliaceae) extracts against Colo205 human colon cancer cell lines by SRB assay for the first time. The bulbs and leaves of the plant were sequentially extracted using solvents with increasing polarities (hexane> chloroform>ethyl acetate>methanol>water). Ethyl acetate and chloroform bulb extracts showed potent anticancer activity compared to standard, adriamycin. Both the extracts exhibited total growth inhibition of cell at 20.1 µg/mL and 32.1 µg/mL whereas adriamycin shown 33.1 µg/mL and 50% lethal concentration was found to be 61.5 µg/mL and >80 µg/mL respectively. The active extracts were subjected to GC-MS analysis for identification of phytocompounds and it showed seven and ten major compounds respectively. Therefore, the present study demonstrated that D. nagarjunae can be a promising candidate as an anticancer agent.

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