scholarly journals SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma

2021 ◽  
Vol 11 (12) ◽  
pp. 1638
Author(s):  
Domantas Vaiciulis ◽  
Alvita Vilkeviciute ◽  
Greta Gedvilaite ◽  
Brigita Glebauskiene ◽  
Loresa Kriauciuniene ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Clinical Manifestations ◽  
Serum Levels ◽  
Control Group ◽  
Adaptive Responses ◽  
Blood Leukocytes ◽  
Ample Evidence ◽  
Significant Difference ◽  
Commercial Kits ◽  
And Control

The aim of the study was to find the association between SIRT1 concentration, SIRT1 rs3758391, rs3818292, rs7895833 polymorphisms and clinical manifestations of pituitary adenoma (PA). The study included 108 patients with PA and 216 healthy individuals. Using commercial kits, DNA was extracted from peripheral blood leukocytes. To determine the PA and control group subjects' genotypes was used real-time PCR method, for SIRT concentration measurement we used ELISA method. The statistical data analysis was completed using the "IBM SPSS Statistics 20.0" software. Results: We performed statistical analysis of SNPs in the patient and healthy controls and patients' subgroups and found statistically significant differences in rs7895833 genotype (A/A, A/G, G/G) distributions between the active PA and control groups (67.9%, 24.6%, 5.7% vs. 72.2%, 27.3%, 0.5%; p = 0.02) Also, the results showed that the rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A (OR = 13.95% CI: 1.314–128.632; p = 0.028) and both A/A and A/G genotypes (OR = 12.9; 95% CI: 1.314–126.624; p = 0.028). There is ample evidence that SIRT1 in the pituitary and other target organs modifies the synthesis, secretion, and activity of hormones to trigger adaptive responses, thus we decided to include this in our study. When determining the serum concentration of SIRT1, we did not find a statistically significant difference between the PA group and the control group. SIRT1 serum level was statistically significantly higher in women with PA than in healthy control women (1.115 (3.748) vs. 136 (0.211); p = 0.008). To conclude - SIRT1 rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A and both A/A and A/G genotypes. SIRT1 serum levels are higher in women with PA than in healthy women.

Plasma kisspeptin and ghrelin levels in puberty variant cases

2017 ◽  
Vol 30 (5) ◽  
Author(s):  
Erdal Kurnaz ◽  
Yaşar Şen ◽  
Süleyman Aydın
Keyword(s):  
Serum Levels ◽  
Control Group ◽  
Control Groups ◽  
Premature Thelarche ◽  
Premature Adrenarche ◽  
Significant Difference ◽  
And Gender ◽  
And Control ◽  
Relationship Of ◽  
The Relationship

AbstractBackground:The aim of this study was to determine the serum levels of kisspeptin and ghrelin (GAH), as well as the relationship of these two peptides with each other in premature thelarche (PT) and premature adrenarche (PA) cases and to investigate the possibility of using these peptides as markers in the differentiation of puberty disorders.Methods:A PT group aged 1–8 years (n = 40), a PA group aged 1–9 years (n = 23, female/male = 20/3) and control groups consistent with each of the previous groups in terms of age and gender were created for the study. Kisspeptin and ghrelin levels were measured with ELISA methods from blood samples drawn while fasting in the morning.Results:When the PT group was compared with the controls, the plasma kisspeptin levels of the cases were significantly higher than the control group (165.47 ± 15.45 pmol/L, 96.82 ± 12.33 pmol/L, p = 0.005, respectively). Kisspeptin levels in the PA group did not show a difference with the control group (121.36 ± 17.99 pmol/L, 95.52 ± 11.54 pmol/L, p = 0.249, respectively). No significant difference could be found when GAH levels in the PT and PA groups were compared with controls. No significant correlation was found between kisspeptin and GAH levels in the PT and PA groups.Conclusions:Our results indicate that kisspeptin plays an important role in the PT, but GAH is not associated with puberty disorders.

Serum Interleukin-15 in Alopecia Areata and its correlation with dermoscopic findings

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Naziha Hafez Khafagy ◽  
Marwa Salah El Din Zaki ◽  
Aya Mahmoud Hussein
Keyword(s):  
Hair Loss ◽  
Alopecia Areata ◽  
Serum Levels ◽  
Common Finding ◽  
Control Group ◽  
University Hospitals ◽  
Exclamation Mark ◽  
Significant Difference ◽  
Initial Onset ◽  
And Control

Abstract Background Alopecia areata is an autoimmune hair loss which frequently starts in childhood. Its presentation had an extreme variability not only in the time of initial onset but also in the duration, extent, and pattern of hair loss during any given episode of active loss. Moreover, the course of disease is unpredictable, with spontaneous regrowth of hair occurring in 80% of patients within the first year and sudden relapse at any given time. Due to the clinical variability and unpredictable nature of spontaneous regrowth, diagnosis and management may be difficult and challenging. Objective The aim of this study is to evaluate the serum levels of IL-15 in active alopecia areata and correlate them with disease severity and activity according to dermoscopic findings. Methods This case-control study were conducted in Dermatology, Venereology and Andrology department, Ain Shams University Hospitals included 30 patients with different clinical variants of AA, the diagnosis was made via clinical examination and dermoscopic findings. In addition, 30 apparently healthy individuals of matched age and sex as a control group were included in the study. Results Dermoscopic examination among cases showed that the most common dermoscopic findings in patients were vellus hair and yellow dots, while the least common finding was exclamation mark hairs. On comparing serum IL-15 in patients and control groups, it was found that serum levels of IL-15 in patients were significantly higher than those in the control group. There was no statistically significant difference in serum IL-15 levels between patients with negative and positive pull test, nail involvement, or body involvement. Similarly, no statistically significant difference in serum IL-15 levels in patients with various subjective disease activity was detected. However, there was a highly significant difference between serum IL-15 levels in different SALT score groups, with the highest levels being in the S3 group. There was a highly significant difference between IL-15 levels in patients with and without black dots. Also, there was significant difference between IL-15 in patients with and without broken hair, and exclamation mark hair. There was no significant difference in level of IL-15 among patients with and without yellow dots, and with and without vellus hair. Conclusion On the basis of the current study, we can conclude that IL-15 is significantly elevated in AA patients when compared to the control subjects. It is also a possible marker of AA severity. It is positively correlated with dermoscopic findings in AA patients, so dermoscopic findings can be useful in evaluating severity of alopecia areata.

scholarly journals Studying the Frequency of Autoimmune Thyroid Diseases in Duhok Province

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Rozheen I. Hasan ◽  
Amir H. Raziq
Keyword(s):  
Venous Blood ◽  
Serum Levels ◽  
Control Group ◽  
Thyroid Disorder ◽  
Control Groups ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Significant Difference ◽  
The One ◽  
And Control

Thyroid disorders are one of the most frequent disorders affecting endocrine system. The present study aimed to identify the frequency of thyroid disorder in our locality at Duhok city. To do so, a total of one hundred patients and one hundred apparently healthy control individuals were enrolled in this study. Samples of venous blood (5 ml) were withdrawn from patients and control subjects and analysed. Clinical assessment was performed by a specialist and each patient provided with a document supporting preliminary clinical diagnosis. Measurements of serum TSH, T3, T4, and anti-thyroperoxidase antibodies (anti-TPO antibodies) serum levels were performed. All assays were conducted at Duhok central health laboratory. Out of the one hundred patients and the one hundred control groups, forty-two and thirty candidates were randomly selected, respectively. The mean TSH serum levels of 25.87 (uIU/ml) for the patients significantly differ from that of the control group 3.55 (uIU/ml) (p<0.001). However, T3 and T4 serum levels indicated no significant difference between the two categories. Moreover, there was statisticaly significant difference (p< 0.0001) between the patients and control groups when their mean serum anti-TPO concentrations were compared.  In conclusion, considerable number of subjects appeared to be candidates for deranged thyroid functions and is susceptible to autoimmune thyroid disorder.

scholarly journals Aqueous Levels of Pigment Epithelium-Derived Factor and Macular Choroidal Thickness in High Myopia

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Wei Chen ◽  
Yubo Guan ◽  
Guanghui He ◽  
Zhiwei Li ◽  
Hui Song ◽  
...  
Keyword(s):  
High Myopia ◽  
Choroidal Thickness ◽  
Pigment Epithelium ◽  
Serum Levels ◽  
Enhanced Depth Imaging ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Pigment Epithelium Derived Factor ◽  
Significant Difference ◽  
And Control

Purpose. To investigate the correlation between aqueous and serum levels of pigment epithelium-derived factor (PEDF) and macular choroidal thickness in high myopia patients, both with and without choroidal neovascularization (CNV).Methods. Serum and aqueous levels of PEDF were measured by enzyme-linked immunosorbent assay in 36 high myopia patients (36 eyes) with no CNV (non-CNV group), 14 high myopia patients (14 eyes) with CNV (CNV group), and 42 nonmyopia patients (42 eyes) (control group). Macular choroidal thickness was measured by enhanced-depth imaging optical coherence tomography.Results. Aqueous levels of PEDF were significantly higher in CNV group compared with non-CNV (P<0.001) and control (P<0.001) groups. Macular choroidal thicknesses were significantly decreased in the non-CNV and CNV groups compared with the control (P<0.001) group. A statistically significant difference (P=0.012) was found between the CNV and non-CNV groups. There was a positive correlation between aqueous PEDF and macular choroidal thickness in the non-CNV group (P=0.005), but no correlation with the CNV group. No correlation between serum PEDF and macular choroidal thickness was detected in the three groups.Conclusion. Variations in aqueous PEDF levels coincide with changes in macular choroidal thickness in high myopia patients with no CNV, while no such relationship exists in high myopia patients with CNV.

scholarly journals Clinical study of pituitary tumors with EEA resection and its influence on the expression of tumor necrosis factor-α, interleukin-6, and interleukin-12 in patients

2019 ◽  
Vol 17 ◽  
pp. 205873921983704
Author(s):  
Wentao Wang ◽  
Dequan Zhong ◽  
Hua Cheng ◽  
Chengfu Ji ◽  
Zhouming Shen ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Operation Time ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Interleukin 12 ◽  
Control Group ◽  
Observation Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Factor Α

The aim of this study is to investigate the efficacy of expanded endonasal approaches (EEAs) in the treatment of pituitary adenoma, and the effects of serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-12 (IL-12) in patients were further analyzed. A total of 80 cases of patients with pituitary adenoma admitted to our hospital from January 2013 to May 2015 were randomly divided into the observation group and control group, with 40 cases in each group. The observation group was treated with EEA technique, while the control group was treated with transcranial microsurgery. The prolactin (PRL), growth hormone (GH) and other endocrine hormone indexes, as well as inflammatory factors such as TNF-α, IL-6, and IL-12 were compared before and 1 week after the operation between the two groups. Moreover, the scores of Karnofsky performance status (KPS) and Mini-Mental State Examination (MMSE) at preoperative and postoperative 1 week were also compared between the two groups. The operation time, hospitalization time, and postoperative complications were compared between the two groups. And the patients were followed up for 2 years to observe the recurrence rate. The operation time and hospital stay in the observation group were 62.8 ± 9.3 min and 12.5 ± 2.1 days, respectively, while the operation time and length of stay in the control group were 105.6 ± 15.7 min and 18.2 ± 3.4 days, respectively. The operation time and hospitalization time were shorter than those in the control group, and the differences were statistically significant ( P < 0.05). Before surgery, there was no significant difference in serum levels of PRL and GH between the two groups ( P > 0.05). At 1 week after surgery, the levels of PRL and GH in the two groups were significantly lower than those before surgery ( P < 0.05). And the serum levels of PRL and GH in the observation group were significantly lower than those in the observation group at 1 week after the operation ( P < 0.05). Before operation, there was no significant difference between the two groups of KPS and MMSE scores ( P > 0.05). At 1 week after operation, the scores of KPS and MMSE in both groups were significantly higher than those before the operation ( P < 0.05), and the scores of KPS and MMSE in the observation group were significantly higher than those in the control group ( P < 0.05). Furthermore, there was no significant difference in the serum levels of IL-6, IL-12, and TNF-α between the two groups before operation ( P > 0.05). At 1 week after surgery, the serum levels of IL-6, IL-12, and TNF-α in the two groups were significantly lower than those before surgery ( P < 0.05), while the serum levels of IL-6, IL-12, and TNF-α in the observation group were evidently lower than those in the control group at 1 week after the operation. Besides, the incidence of postoperative complications in the observation group was 7.5%, which was significantly lower than that in the control group (17.5%) ( P < 0.05). All the patients in the two groups were followed up for 2 years. And there was no recurrence in the observation group and one case in the control group. The therapeutic effect of EEAs on pituitary adenoma is better. It can improve the level of high endocrine hormone in patients with pituitary adenoma, improve the functional status of the patients, reduce the serum level of inflammatory factors, and shorten the hospital stay. Meanwhile, it also has the characteristics of fewer complications and low recurrence rate, so it can be popularized in clinical practice.

scholarly journals Serum Prolactin Levels in Psoriasis Vulgaris

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Farhad Handjani ◽  
Nasrin Saki ◽  
Iman Ahrari ◽  
Mehdi Ebrahimi ◽  
Mohammad Mehdi Khorrami ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Normal Population ◽  
Venous Blood ◽  
Human Keratinocytes ◽  
Serum Levels ◽  
Serum Prolactin ◽  
Control Group ◽  
Significant Difference ◽  
Dominant Feature ◽  
And Control

Background. Psoriasis is a chronic inflammatory skin disease affecting approximately 1–3% of Caucasians. Prolactin has proliferative effects on human keratinocytes, a dominant feature of psoriasis, and it is thought that this hormone may play a role in the pathogenesis of the disease. This study was conducted to confirm or refute these findings in order to better understand the disease pathogenesis. Methods. The subjects were 90 individuals aged between 15 and 47 years. They were divided into three groups of 30 individuals each: psoriatic patients, atopic dermatitis patients, and control group. A questionnaire was filled regarding their demographic and medical history. All of the study subjects underwent venous blood sampling (5 mL), and serum TSH and prolactin levels were checked. Subjects with abnormal TSH were omitted. Results. None of the patients in the study had raised prolactin, and there was no significant difference in the serum prolactin level between patients with psoriasis and atopic dermatitis and the control group. There was no relationship between the severity of psoriasis and serum levels of prolactin. Conclusion. Prolactin does not seem to play a role in the pathogenesis of psoriasis as its serum levels are comparable with atopic dermatitis patients and that of the normal population.

scholarly journals Clinical significance of serum AMH and INHB in girls with precocious puberty

2021 ◽  
Author(s):  
Qingxu Liu ◽  
Xiaoqin Yin ◽  
Pin Li
Keyword(s):  
Clinical Significance ◽  
Normal Control ◽  
Precocious Puberty ◽  
Roc Analysis ◽  
Clinical Manifestations ◽  
Tanner Stage ◽  
Serum Levels ◽  
Normal Control Group ◽  
Control Group ◽  
Significant Difference

Abstract Background: Precocious puberty is the second sexual characteristic of girls before 8 years old. The diagnosis of central precocious puberty (CPP) needs to evaluate ovarian function, and ultrasound examination is an auxiliary means for the evaluation of ovary at present. The serum levels of AMH and INHB might be markers of evaluation of ovary according the former research. We investigated the clinical features, serum sex hormones, serum levels of AMH and INHB in 184 girls with precocious puberty, which provided deeper insight into the clinical significance of AMH and INHB in female precocious puberty. Methods: We evaluated 184 girls with precocious puberty at the Department of Endocrinology of Shanghai Children's Hospital from 2017 to 2021, which was consisted of PT, Tanner stage 2 CPP and Tanner stage 3 CPP. We analysed clinical data from the patients including clinical manifestations, AMH, INHB and other hormone levels, and we analysed AMH and INHB in normal control group either.Results: The PT group (P = 0.031) and Tanner stage 2 CPP group (P = 0.006) exhibited significantly higher AMH level than that in normal control group. AMH level showed no significant difference among PT group, Tanner stage 2 CPP group and Tanner stage 3 CPP group. The Tanner stage 2 CPP group exhibited significantly higher level of INHB than that in Tanner stage 3 CPP group (P = 0.013) and normal control group (P = 0.007). AMH and INHB were positively correlated in the four groups, especially in the PT group (r = 0.694**, P < 0.01). AMH and basal LH were positively correlated in PT group (r = 0.296*, P < 0.01). AMH or INHB showed no correlation with chronical age, bone age, uterine volume, ovarian volume, BMI, E2, SHBG, peak LH, basal FSH, peak FSH, IGF-1 or IGF-BP3. The ROC analysis showed that the AUC of AMH or INHB was relatively low. Conclusions: In this research, the serum AMH and INHB in 184 girls with precocious puberty were analysed. The PT group and Tanner stage 2 CPP group exhibited significantly higher AMH level than that in normal control group. The Tanner stage 2 CPP group exhibited significantly higher level of INHB than that in Tanner stage 3 CPP group and normal control group. AMH and INHB were positively correlated in the four groups, especially in the PT group. ROC analysis showed that the diagnostic performance of PT or CPP using AMH or INHB was weak.

scholarly journals Responses of Visfatin and Resistin Concentration to Different Aerobic Training Intensities Protocols

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Keyvan Hejazi ◽  
Seyyed Reza Attarzadeh Hosseini ◽  
Mehrdad Fathi ◽  
Mohammad Mosaferi Ziaaldini
Keyword(s):  
High Intensity ◽  
Metabolic Regulation ◽  
Aerobic Training ◽  
Serum Levels ◽  
Male Rats ◽  
Moderate Intensity ◽  
Control Group ◽  
Physiological Processes ◽  
Significant Difference ◽  
And Control

Background: Visfatin and resistin are adipokines involved in metabolic regulation and physiological processes. Objectives: This study aimed to investigate the effects of eight weeks of aerobic training with different intensities on serum levels of resistin and visfatin in obese male rats. Methods: 24 adult obese rats (weight: 250 to 300 gr, BMI > 30 g/cm2) divided into three groups: aerobic training with 28 m/min (moderate intensity), aerobic training with 34 m/min (high intensity) and, control group. All training groups did exercise training for 8 weeks (5 sessions/per-week for 60 minutes per session). 48 hours after the training period, the level of serum visfatin and resistin levels was measured. Results: Unlike the levels of resistin concentration (P = 0.001) in different groups, there was no significant difference between visfatin levels in all three groups. Resistin concentration was not significantly changed in the moderate-intensity group compared to the control group, (185.10 ± 12.85 vs. 202.76 ± 17.39) (P = 0.05). There was a significant difference between the high intensity and control groups (P = 0.001). There was no significant difference in visfatin concentration in MI and HI groups compared to the control group (P > 0.05). Conclusions: There is a significant difference in the levels of resistin concentration in different groups. There was no significant difference between visfatin levels in all three groups. Therefore, it seems that aerobic training with high intensity has a better effect on the prevention of obesity and cardiovascular disease.

scholarly journals Preoperative Serum Levels of Mesothelin in Patients with Colon Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Özgür Bostancı ◽  
Özgür Kemik ◽  
Ahu Kemik ◽  
Muharrem Battal ◽  
Uygar Demir ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Perineural Invasion ◽  
Tumor Grade ◽  
Serum Levels ◽  
Control Group ◽  
Control Groups ◽  
Colonoscopic Examination ◽  
Preoperative Serum ◽  
Significant Difference ◽  
And Control

Background. Screening for biochemical markers is important for diagnosing colon cancer. In this study, the reliability of serum mesothelin levels as a potential diagnostic and screening instrument was evaluated concerning colon cancer.Methods. Ninety-five patients who had undergone colonoscopic examination and who were diagnosed with colon cancer were included in the study. The serum mesothelin levels were measured with the ELISA kits and were evaluated in terms of significant difference when compared between colon cancer and control group.Results. Patients with colon cancer had significantly higher mesothelin serum levels(P<0.001)than the control groups. We found significant associations between serum levels and tumor grade, perineural invasion, and vascular invasion (resp.,P<0.001).Conclusion. Evaluating the serum levels of mesothelin has a potential to detect and screen the colon cancer in affected patients. Our data suggest that mesothelin exhibits effects towards colon cancer and serves as a biomarker for this deadly disease.

scholarly journals Vitamin E Is a Nephroprotectant Agent in Male but Not in Female in a Model of Cisplatin-Induced Nephrotoxicity

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sima Jilanchi ◽  
Mehdi Nematbakhsh ◽  
Mehrnoosh Bahadorani ◽  
Ardeshir Talebi ◽  
Fatemeh Eshraghi-Jazi ◽  
...  
Keyword(s):  
Vitamin E ◽  
Kidney Tissue ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Weight Changes ◽  
Body Weight Changes ◽  
Significant Difference ◽  
And Control

Background. The role of gender for nephroprotectant agent such as vitamin E in cisplatin- (CP-) induced nephrotoxicity has not been documented yet. Methods. One group from each gender of Wistar rats received a single dose of CP (7 mg/kg; i.p) and was treated with vitamin E (1 g/kg/day) for 7 days, and they were compared with similar gender in the control group. Results. The serum levels of blood urea nitrogen (BUN) and creatinine (Cr) in male animals treated with CP was not different from the control group, but it was significantly different in the female rats (P<0.05). The CP-induced damage intensity in male kidney tissue was not significantly different between the CP-treated and control groups, but this was not the case in female, indicating that the tissue damage in female is significantly different from the control group (P<0.05). No significant difference in serum levels of magnesium (Mg), nitrite, malondialdehyde (MDA), and lactate dehydrogenase (LDH) was seen between the genders. Kidney weight and body weight changes were statistically significant in both genders (P<0.05). Significant difference was observed in uterus weight between the two groups of female (P<0.05). Conclusion. Vitamin E may prevent CP-induced nephrotoxicity in male, but possibly it has not such nephroprotectant effect in female.

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