Bioactive Compounds and Evaluation of Antioxidant, Cytotoxic and Cytoprotective Effects of Murici Pulp Extracts (Byrsonima crassifolia) Obtained by Supercritical Extraction in HepG2 Cells Treated with H2O2

The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H2O2. The murici pulp was subjected to sequential extraction with supercritical CO2 and CO2+ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications.

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Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

Current Analytical Chemistry ◽

10.2174/1573411016999200729111951 ◽

2020 ◽

Vol 16 ◽

Author(s):

Natasa P. Kalogiouri ◽

Natalia Manousi ◽

Erwin Rosenberg ◽

George A. Zachariadis ◽

Victoria F. Samanidou

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

High Performance ◽

Unsaturated Fatty Acids ◽

Nutritional Deficiencies ◽

Analytical Technique ◽

High Volatility ◽

Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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Low density lipoprotein from humans supplemented with n-3 fatty acids depresses both LDL receptor activity and LDLr mRNA abundance in HepG2 cells.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)41429-4 ◽

1992 ◽

Vol 33 (5) ◽

pp. 647-658

Author(s):

S Lindsey ◽

A Pronczuk ◽

KC Hayes

Keyword(s):

Fatty Acids ◽

Hepg2 Cells ◽

Low Density Lipoprotein ◽

Ldl Receptor ◽

Density Lipoprotein ◽

Mrna Abundance ◽

Receptor Activity ◽

Low Density

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Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using Hepg2 Cell Line

Molecules ◽

10.3390/molecules26154593 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4593

Author(s):

Deepthi Venkatachalapathy ◽

Chandan Shivamallu ◽

Shashanka Prasad ◽

Gopenath Thangaraj Saradha ◽

Parthiban Rudrapathy ◽

...

Keyword(s):

Liver Cancer ◽

Vitis Vinifera ◽

Bioactive Compounds ◽

Camellia Sinensis ◽

Hepg2 Cells ◽

Human Liver ◽

Withania Somnifera ◽

Antioxidant Properties ◽

Annexin V ◽

Human Liver Cancer

The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.

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Bee collected pollen as a value-added product rich in bioactive compounds and unsaturated fatty acids: A comparative study from Turkey and Romania

LWT ◽

10.1016/j.lwt.2021.111925 ◽

2021 ◽

pp. 111925

Author(s):

Rodica Mărgăoan ◽

Aslı Özkök ◽

Şaban Keskin ◽

Nazlı Mayda ◽

Adriana Cristina Urcan ◽

...

Keyword(s):

Fatty Acids ◽

Comparative Study ◽

Bioactive Compounds ◽

Unsaturated Fatty Acids ◽

Value Added ◽

A Value

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Bioactive Compounds in Salicornia patula Duval-Jouve: A Mediterranean Edible Euhalophyte

Foods ◽

10.3390/foods10020410 ◽

2021 ◽

Vol 10 (2) ◽

pp. 410

Author(s):

Irene Sánchez-Gavilán ◽

Esteban Ramírez ◽

Vicenta de la Fuente

Keyword(s):

Fatty Acids ◽

Bioactive Compounds ◽

Mediterranean Area ◽

Biological Properties ◽

Total Phenolic ◽

Tinto River ◽

New Findings ◽

High Concentrations ◽

Salicornia Patula ◽

First Time

Many halophytes have great nutritional and functional potential, providing chemical compounds with biological properties. Salicornia patula Duval-Jouve is a common euhalophyte from saline Mediterranean territories (Spain, Portugal, France, and Italy). In the present work we quantified for the first time the bioactive compounds in S. patula (total phenolic compounds and fatty acids), from Iberian Peninsula localities: littoral-coastal Tinto River basin areas (southwest Spain, the Huelva province), and mainland continental territories (northwest and central Spain, the Valladolid and Madrid provinces). Five phenolic acids including caffeic, coumaric, veratric, salicylic, and transcinnamic have been found with differences between mainland and coastal saltmarshes. S. patula contain four flavonoids: quercetin-3-O-rutinoside, kaempferol/luteolin, apigenin 7-glucoside, and pelargonidin-3-O-rutinoside. These last two glycosylated compounds are described for the first time in this genus of Chenopodiaceae. The fatty acid profile described in S. patula stems contains palmitic, oleic, and linoleic acids in high concentrations, while stearic and long-chain fatty acids were detected in low amounts. These new findings confirm that S. patula is a valuable source of bioactive compounds from Mediterranean area.

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Colour, fatty acids, bioactive compounds, and total antioxidant capacity in commercial cocoa beans (Theobroma cacao L.)

LWT ◽

10.1016/j.lwt.2021.111629 ◽

2021 ◽

pp. 111629

Author(s):

Fernando Ramos-Escudero ◽

Sandra Casimiro-Gonzales ◽

África Fernández-Prior ◽

Keidy Cancino Chávez ◽

José Gómez-Mendoza ◽

...

Keyword(s):

Fatty Acids ◽

Antioxidant Capacity ◽

Bioactive Compounds ◽

Total Antioxidant Capacity ◽

Theobroma Cacao ◽

Cocoa Beans ◽

Total Antioxidant ◽

Theobroma Cacao L

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Identification of the Fatty Acids Profiles in Supercritical CO2 Fluid and Soxhlet Extraction of Samara Oil from Different Cultivars of Elaeagnus mollis Diels Seeds

Journal of Food Composition and Analysis ◽

10.1016/j.jfca.2021.103982 ◽

2021 ◽

pp. 103982

Author(s):

Jianlou Mu ◽

Gang Wu ◽

Zhizhou Chen ◽

Charles S. Brennan ◽

Khanh Tran ◽

...

Keyword(s):

Fatty Acids ◽

Supercritical Co2 ◽

Soxhlet Extraction ◽

Supercritical Co2 Fluid ◽

Elaeagnus Mollis

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QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13480 ◽

2016 ◽

pp. 183

Author(s):

Parameswari P ◽

Devika Rengaswamy

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Quantitative Analysis ◽

Secondary Metabolites ◽

Bioactive Compounds ◽

Leaf Extract ◽

Metabolic Reaction ◽

Quantitative Investigation ◽

Artemisia Nilagirica ◽

Quantitative Examination

ABSTRACT Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on different human diseases. Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments for quantitative investigation of phytochemicals according to standard strategies. Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows separately. Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward extraction and refinement of specific bioactive mixes for human nourishment. Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.

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