scholarly journals Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 846
Author(s):  
Meiling Tian ◽  
Daotong Li ◽  
Chen Ma ◽  
Yu Feng ◽  
Xiaosong Hu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Fiber ◽  
Intestinal Microbiota ◽  
Short Chain Fatty Acids ◽  
Soluble Dietary Fiber ◽  
Insoluble Dietary Fiber ◽  
Tnf Α ◽  
Barley Leaf ◽  
Inflammatory Bowel ◽  
Secondary Bile Acids

Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation. However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF). In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice. The mice were fed 1.52% BLIDF-supplemented diet for 28 days. Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice. BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes. Importantly, the anti-colitis effects of BLIDF were abolished when the intestinal microbiota was depleted by antibiotics. Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces. Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice. Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.

scholarly journals Effects of Dietary Cereal and Protein Source on Fiber Digestibility, Composition, and Metabolic Activity of the Intestinal Microbiota in Weaner Piglets

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 109
Author(s):  
Carola Ellner ◽  
Anna G. Wessels ◽  
Jürgen Zentek
Keyword(s):  
Dietary Fiber ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Metabolic Activity ◽  
Short Chain Fatty Acids ◽  
Rapeseed Meal ◽  
Insoluble Dietary Fiber ◽  
Fiber Digestibility ◽  
Degrading Bacteria ◽  
Fermentative Activity

This study aimed to investigate the effect of fiber-rich rye and rapeseed meal (RSM) compared to wheat and soybean meal (SBM) on fiber digestibility and the composition and metabolic activity of intestinal microbiota. At weaning, 88 piglets were allocated to four feeding groups: wheat/SBM, wheat/RSM, rye/SBM, and rye/RSM. Dietary inclusion level was 48% for rye and wheat, 25% for SBM, and 30% for RSM. Piglets were euthanized after 33 days for collection of digesta and feces. Samples were analyzed for dry matter and non-starch-polysaccharide (NSP) digestibility, bacterial metabolites, and relative abundance of microbiota. Rye-based diets had higher concentrations of soluble NSP than wheat-based diets. RSM-diets were higher in insoluble NSP compared to SBM. Rye-fed piglets showed a higher colonic and fecal digestibility of NSP (p < 0.001, p = 0.001, respectively). RSM-fed piglets showed a lower colonic and fecal digestibility of NSP than SBM-fed piglets (p < 0.001). Rye increased jejunal and colonic concentration of short-chain fatty acids (SCFA) compared to wheat (p < 0.001, p = 0.016, respectively). RSM-fed pigs showed a lower jejunal concentration of SCFA (p = 0.001) than SBM-fed pigs. Relative abundance of Firmicutes was higher (p = 0.039) and of Proteobacteria lower (p = 0.002) in rye-fed pigs compared to wheat. RSM reduced Firmicutes and increased Actinobacteria (jejunum, colon, feces: p < 0.050), jejunal Proteobacteria (p = 0.019) and colonic Bacteroidetes (p = 0.014). Despite a similar composition of the colonic microbiota, the higher amount and solubility of NSP from rye resulted in an increased fermentative activity compared to wheat. The high amount of insoluble dietary fiber in RSM-based diets reduced bacterial metabolic activity and caused a shift toward insoluble fiber degrading bacteria. Further research should focus on host–microbiota interaction to improve feeding concepts with a targeted use of dietary fiber.

scholarly journals Soluble Dietary Fiber, One of the Most Important Nutrients for the Gut Microbiota

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6802
Author(s):  
Zhi-Wei Guan ◽  
En-Ze Yu ◽  
Qiang Feng
Keyword(s):  
Gut Microbiota ◽  
Human Health ◽  
Dietary Fiber ◽  
Physicochemical Characteristics ◽  
Dietary Fibers ◽  
Soluble Dietary Fiber ◽  
Physiological Functions ◽  
Clinical Interventions ◽  
Insoluble Dietary Fiber ◽  
Gastrointestinal Health

Dietary fiber is a widely recognized nutrient for human health. Previous studies proved that dietary fiber has significant implications for gastrointestinal health by regulating the gut microbiota. Moreover, mechanistic research showed that the physiological functions of different dietary fibers depend to a great extent on their physicochemical characteristics, one of which is solubility. Compared with insoluble dietary fiber, soluble dietary fiber can be easily accessed and metabolized by fiber-degrading microorganisms in the intestine and produce a series of beneficial and functional metabolites. In this review, we outlined the structures, characteristics, and physiological functions of soluble dietary fibers as important nutrients. We particularly focused on the effects of soluble dietary fiber on human health via regulating the gut microbiota and reviewed their effects on dietary and clinical interventions.

scholarly journals Fiber substrates in the nutrition of weaned piglets – a review

2017 ◽  
Vol 17 (3) ◽  
pp. 627-644 ◽  
Author(s):  
Marianna Flis ◽  
Wiesław Sobotka ◽  
Zofia Antoszkiewicz
Keyword(s):  
Dietary Fiber ◽  
Wheat Bran ◽  
Intestinal Barrier ◽  
Protein Digestibility ◽  
Short Chain Fatty Acids ◽  
Intestinal Barrier Function ◽  
Weaned Piglets ◽  
Oat Hulls ◽  
Insoluble Dietary Fiber ◽  
Diarrhea Incidence

Abstract The present review summarizes the results of 37 experiments in which different types and levels (from 0.5 to 29.7%) of fibrous supplements were used in the formulation of diets for weaned piglets. Diets were supplemented with different sources of insoluble dietary fiber (iDF), soluble dietary fiber (sDF), or mixed DF sources. Most of the applied DF sources decreased the ileal and fecal organic matter digestibility, and they often lowered crude protein digestibility. A moderate addition (1.5-8%) of iDF sources increased average daily feed intake (ADFI) and, frequently, average daily gains (ADG). Sources of sDF as well as high inclusion levels of fiber-rich feeds tended to decrease ADFI and ADG. Improved fecal consistency, decreased diarrhea incidence and antibiotic interventions were confirmed in piglets fed diets with added lignocellulose, cooked or raw oat hulls and wheat bran. The dietary inclusion of iDF rather than sDF sources improved gastrointestinal tract (GIT) development, enzyme activity and gut morphology. An increase in the counts of beneficial gut microbiota and the concentrations of short-chain fatty acids was stimulated by diets with addition iDF or sDF sources. Such diets also slowed down proteolytic fermentation which negatively affects the colonic mucosa. Some research findings indicate that iDF sources improve intestinal barrier function. The analyzed experimental data suggest that the addition of 1.5-2% of a lignocellulose preparation, 2% of oat hulls, 4-8% of coarse wheat bran to diets for weaned piglets may be recommended to promote GIT development and health, and to improve growth performance.

Rapid Integrated Method for Total Dietary Fiber

2021 ◽  
Keyword(s):  
Dietary Fiber ◽  
Resistant Starch ◽  
High Performance ◽  
Enzymatic Digestion ◽  
Soluble Dietary Fiber ◽  
Total Dietary Fiber ◽  
Food Ingredients ◽  
Insoluble Dietary Fiber ◽  
Integrated Method ◽  
Intestinal Digestion

This method determines total dietary fiber (TDF) in foods and food ingredients, as defined by Codex Alimentarius. The method measures soluble and insoluble dietary fiber, including resistant starch, as well as nondigestible oligosaccharides. In this method, enzymatic digestion is used to simulate human intestinal digestion. Insoluble dietary fiber (IDF) and soluble dietary fiber that precipitates in 78% ethanol (SDFP) are separated by filtration and quantified gravimetrically. Additionally, highly soluble oligosaccharides (SDFS) are quantified by chromatographic separation. TDF is reported as the sum of the gravimetric and high-performance liquid chromatography (HPLC) results. The digestion and chromatographic conditions of this method have been modified from those of AACC Approved Methods 32-45.01 and 32-50.01 in an attempt to better simulate human digestion and to allow for more exact quantitation.

scholarly journals Insoluble dietary fiber from soy hulls regulates the gut microbiota in vitro and increases the abundance of bifidobacteriales and lactobacillales

2019 ◽  
Vol 57 (1) ◽  
pp. 152-162 ◽  
Author(s):  
Lina Yang ◽  
Yafan Zhao ◽  
Jinghang Huang ◽  
Hongyun Zhang ◽  
Qian Lin ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Fiber ◽  
Insoluble Dietary Fiber ◽  
Soy Hulls

scholarly journals Dietary lipids, gut microbiota and lipid metabolism

2019 ◽  
Vol 20 (4) ◽  
pp. 461-472 ◽  
Author(s):  
Marc Schoeler ◽  
Robert Caesar
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Liver Disease ◽  
Gut Microbiota ◽  
Short Chain Fatty Acids ◽  
Dietary Lipids ◽  
Lipid Levels ◽  
And Function ◽  
Host Metabolism ◽  
Secondary Bile Acids

Abstract The gut microbiota is a central regulator of host metabolism. The composition and function of the gut microbiota is dynamic and affected by diet properties such as the amount and composition of lipids. Hence, dietary lipids may influence host physiology through interaction with the gut microbiota. Lipids affect the gut microbiota both as substrates for bacterial metabolic processes, and by inhibiting bacterial growth by toxic influence. The gut microbiota has been shown to affect lipid metabolism and lipid levels in blood and tissues, both in mice and humans. Furthermore, diseases linked to dyslipidemia, such as non-alcoholic liver disease and atherosclerosis, are associated with changes in gut microbiota profile. The influence of the gut microbiota on host lipid metabolism may be mediated through metabolites produced by the gut microbiota such as short-chain fatty acids, secondary bile acids and trimethylamine and by pro-inflammatory bacterially derived factors such as lipopolysaccharide. Here we will review the association between gut microbiota, dietary lipids and lipid metabolism

scholarly journals Inflammatory and Microbiota-Related Regulation of the Intestinal Epithelial Barrier

2021 ◽  
Vol 8 ◽  
Author(s):  
Giovanni Barbara ◽  
Maria Raffaella Barbaro ◽  
Daniele Fuschi ◽  
Marta Palombo ◽  
Francesca Falangone ◽  
...  
Keyword(s):  
Immune System ◽  
Gut Microbiota ◽  
Cell Damage ◽  
Short Chain Fatty Acids ◽  
The Body ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Inflammatory Bowel ◽  
Irritable Bowel

The intestinal epithelial barrier (IEB) is one of the largest interfaces between the environment and the internal milieu of the body. It is essential to limit the passage of harmful antigens and microorganisms and, on the other side, to assure the absorption of nutrients and water. The maintenance of this delicate equilibrium is tightly regulated as it is essential for human homeostasis. Luminal solutes and ions can pass across the IEB via two main routes: the transcellular pathway or the paracellular pathway. Tight junctions (TJs) are a multi-protein complex responsible for the regulation of paracellular permeability. TJs control the passage of antigens through the IEB and have a key role in maintaining barrier integrity. Several factors, including cytokines, gut microbiota, and dietary components are known to regulate intestinal TJs. Gut microbiota participates in several human functions including the modulation of epithelial cells and immune system through the release of several metabolites, such as short-chain fatty acids (SCFAs). Mediators released by immune cells can induce epithelial cell damage and TJs dysfunction. The subsequent disruption of the IEB allows the passage of antigens into the mucosa leading to further inflammation. Growing evidence indicates that dysbiosis, immune activation, and IEB dysfunction have a role in several diseases, including irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and gluten-related conditions. Here we summarize the interplay between the IEB and gut microbiota and mucosal immune system and their involvement in IBS, IBD, and gluten-related disorders.

scholarly journals Interplay and cooperation of Helicobacter pylori and gut microbiota in gastric carcinogenesis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Seyedeh Zahra Bakhti ◽  
Saeid Latifi-Navid
Keyword(s):  
Helicobacter Pylori ◽  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Bacterial Species ◽  
Short Chain Fatty Acids ◽  
Gastric Carcinogenesis ◽  
Oncogenic Signaling ◽  
Comprehensive Overview ◽  
H Pylori ◽  
Oncogenic Signaling Pathways

AbstractChronic Helicobacter pylori infection is a critical risk factor for gastric cancer (GC). However, only 1–3 % of people with H. pylori develop GC. In gastric carcinogenesis, non-H. pylori bacteria in the stomach might interact with H. pylori. Bacterial dysbiosis in the stomach can strengthen gastric neoplasia development via generating tumor-promoting metabolites, DNA damaging, suppressing antitumor immunity, and activating oncogenic signaling pathways. Other bacterial species may generate short-chain fatty acids like butyrate that may inhibit carcinogenesis and inflammation in the human stomach. The present article aimed at providing a comprehensive overview of the effects of gut microbiota and H. pylori on the development of GC. Next, the potential mechanisms of intestinal microbiota were discussed in gastric carcinogenesis. We also disserted the complicated interactions between H. pylori, intestinal microbiota, and host in gastric carcinogenesis, thus helping us to design new strategies for preventing, diagnosing, and treating GC.

scholarly journals P053 Overabundance of Lactobacillus species in gut microbiota of IBD patients

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S160-S161
Author(s):  
D Khusnutdinova ◽  
M Markelova ◽  
M Siniagina ◽  
E Boulygina ◽  
S Abdulkhakov ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Active Phase ◽  
Bioinformatic Analysis ◽  
Lactobacillus Species ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Shotgun Metagenomic Sequencing ◽  
Inflammatory Bowel ◽  
Stool Samples

Abstract Background Changes in the composition of gut microbiota, and their metabolic pathways, are important factors in the pathogenesis of inflammatory bowel disease (IBD). Many clinical trials have shown that taking probiotics based on Lactobacillus has a positive effect on patients with IBD. However, Lactobacillus should be used more carefully during the active phase of IBD, since some strains can negatively affect the pathogenesis of the disease1,2. The aim of this study was to assess the diversity of Lactobacillus species in the gut microbiome of IBD patients and healthy volunteers. Methods In the study, 62 stool samples from healthy people, 31 from patients with Crohn’s disease (CD), and 34 - ulcerative colitis (UC) in active phase were analyzed. DNA was isolated using the QIAamp Fast DNA Stool Mini Kit (Qiagen, USA) following with shotgun metagenomic sequencing the NextSeq 500 (project #0671-2020-0058). Bioinformatic analysis was performed with the MetaPhlAn2 package. Results An increased relative abundance of Lactobacillus was found in patients with IBD (3.2% ± 6.6% in CD and 1.6% ± 2.8 in UC) compared to healthy individuals (0.3% ± 1.2%, p&lt;0.05). In the control group, Lactobacillus were absent in 41% of samples and 1–5 species were found in 58% of samples. Most CD and UC patients are characterized by the presence of 3 to 5 species of Lactobacillus (38% and 31%, respectively). For 23% of CD patients and 26% of UC patients, 6 to 9 types of Lactobacillus were found. Some patients with IBD have more than 10 different types of Lactobacillus in the gut microbiota (Fig.1). The intestinal microbiota in IBD patients is characterized by an increased abundance of several species: L. salivarius, L. gasseri, L. mucosae, as well as L. casei paracasei in patients with CD and L. vaginalis in patients with UC (Fig.2). Conclusion The composition of the intestinal microbiota of IBD patients differs significantly in terms of Lactobacillus proportion and species diversity. Overabundance of five Lactobacillus species could be associated with the active phase of IBD. References

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