scholarly journals Influence of Elicitation and Drying Methods on Anti-Metabolic Syndrome, and Antimicrobial Properties of Extracts and Hydrolysates Obtained from Elicited Lovage (Levisticum officinale Koch)

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4365
Author(s):  
Anna Jakubczyk ◽  
Urszula Złotek ◽  
Kamila Rybczyńska-Tkaczyk
Keyword(s):  
Metabolic Syndrome ◽  
Antimicrobial Activity ◽  
Biological Activity ◽  
Inhibitory Activity ◽  
Freeze Drying ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Microwave Drying ◽  
Drying Methods ◽  
Ethanolic Extracts

This research aims to investigate the influence of elicitation and drying methods (natural, convection, microwave, and freeze-drying), with jasmonic acid (JA) and yeast extract (YE) on the biological activity of extracts and hydrolysates from lovage (Levisticum officinale Koch) leaves. The results indicate that the highest TPC was determined for hydrolysates obtained from JA-elicited microwave-dried lovage (24.96 mg/gDW). The highest ACE and lipase inhibitory activity was noted for PBS extract obtained from JA-elicited lovage after microwave drying (EC50 = 0.16 and 0.12 mg/mL, respectively). Ethanolic extract from JA-elicited lovage after freeze-drying was characterized by the highest α-amylase inhibitory activity (EC50 = 3.92 mg/mL) and the highest α-glucosidase inhibitory activity (EC50 = 1.43 mg/mL) was noted for hydrolysates from control plants subjected to freeze-drying. The highest antimicrobial activity towards C. albicans yeasts was observed for microwave ethanolic extracts with minimal inhibition (MIC) and lethal (MLC) concentrations of 0.625 and 1.25 mg/mL, respectively.

scholarly journals EVALUATION OF AQUEOUS AND ETHANOLIC EXTRACTS OF SYZYGIUM CARYOPHYLLATUM FOR ANTIBACTERIAL ACTIVITIES

2018 ◽  
Vol 11 (2) ◽  
pp. 235
Author(s):  
Raghunath Pendru ◽  
Jagan Nadipelly ◽  
Jyothinath Kothapalli ◽  
Subbannayya Kotigadde
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

 Objective: It has been well documented that traditional medicinal plants confer considerable antimicrobial activity against various microorganisms.Methods: The present study was designed to evaluate the antimicrobial properties of aqueous and ethanolic extracts of the leaves of Syzygium caryophyllatum against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, and Vibrio cholerae. The aqueous extract was prepared using cold percolation method, and ethanolic extract was prepared in Soxhlet apparatus using ethyl alcohol. Antimicrobial activity is being determined by time-kill assay and minimum inhibitory concentration.Results: From our study, it is found that all tested organisms were sensitive to both aqueous and ethanolic extract of leaves of S. caryophyllatum. The ethanolic extract showed better antibacterial activity against S. aureus and S. typhi when compare to aqueous extract. The phytochemical analysis confirmed the presence of flavonoids, alkaloids, glycosides, steroids, phenols, tannins, and saponins in the ethanolic and aqueous extract of S. caryophyllatum. Conclusion: We hypothesize that these active phytoconstitutents might be responsible for the antimicrobial activity of this plant.

scholarly journals Assessment of antimicrobial activity in vitro of ethanolic extracts of Banisteriopsis anisandra (A. Juss.) B. Gates (Malpighiaceae)

2013 ◽  
Vol 15 (3) ◽  
pp. 431-437 ◽  
Author(s):  
M.S. Pádua ◽  
M.C. Mendes-Costa ◽  
J.M.S. Ferreira ◽  
J.C. Magalhães ◽  
A.H.F. Castro
Keyword(s):  
Antimicrobial Activity ◽  
Fusarium Oxysporum ◽  
Inhibitory Activity ◽  
Ethanolic Extract ◽  
Phytopathogenic Fungi ◽  
Significant Inhibition ◽  
Plant Diseases ◽  
Growth Speed ◽  
Ethanolic Extracts

The aim of this study was to evaluate the antimicrobial activity in vitro of ethanolic extracts of Banisteriopsis anisandra. Tests were performed using the extracts overlay method in the culture medium for phytopathogenic fungi Rhizoctonia solani and Fusarium oxysporum, and disk diffusion for the microorganisms Staphylococcus aureus and Candida albicans. Ethanolic extracts from leaves were prepared by maceration (extract I) and decoction (extract II) at 430.0, 215.0 and 107.5 mg/mL. The growth inhibition of R. solani and F. oxysporum was determined by calculating the mycelia growth speed rate (MGSR) and, in relation C. albicans and S. aureus, it was determined by measuring the inhibition halos. Extracts that caused significant inhibition were also tested at 86.0, 64.5, 43.0 and 21.5 mg/mL for C. albicans and S. aureus. Both extracts showed inhibitory activity on the microorganisms studied. Rizoctonia solani showed lower MGSR in the presence of extract II (107.5 mg/mL) and Fusarium oxysporum showed slight MGSR reduction in the presence of extract I (107.5 mg/mL) and II (107.5 and 215 mg/mL). Ethanolic extracts I and II inhibited the growth of C. albicans, with the highest rates of inhibition observed in the presence of extract II (215.0 mg/mL). For S. aureus, the highest inhibitory activity was observed in the presence of ethanolic extract II, prepared by decoction at 430.0 mg/mL. Results showed a promising antimicrobial activity of extracts of B. anisandra, which may contribute to further studies leading to a future development of medicines to treat human and plant diseases caused by these organisms.

scholarly journals Bioactive Compounds and Antioxidative, Antileukemic and Anti-MMPs Activity of Eleutherococcus Species Cultivated in Poland

2012 ◽  
Vol 7 (11) ◽  
pp. 1934578X1200701
Author(s):  
Daniel Załuski ◽  
Helena D. Smolarz ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Biological Activity ◽  
Cell Line ◽  
Bioactive Compounds ◽  
Inhibitory Activity ◽  
Ethanolic Extract ◽  
Apoptotic Cells ◽  
Biological Studies ◽  
Dpph Activity ◽  
Leaves And Roots

The content of phenols (TPC), and the antioxidative, antileukemic and anti-MMPs properties of Eleutherococcus spp. cultivated in Poland are reported. The highest TPC was found in the spring leaves (20.3 – 37.2 mg/g). The spring leaves and roots of E. divaricatus had the strongest anti-DPPH activity (EC50 1.2 and 1.2 mg/mL), whereas the greatest ability of iron (II) chelating was shown by autumn leaves of E. setchuensis (EC50 0.3 mg/mL), and the ethanolic extract of E. gracilistylus roots showed the strongest lipid antiperoxidation activity (EC50 0.7 mg/mL). The highest number of apoptotic cells was observed in the Jurkat 45 cell line after treatment with the ethanolic extract of E. sessiliflorus roots (27%). Only the chloroform extracts of roots of E. gracilistylus and E. divaricatus showed the inhibitory activity against MMPs. Most of the studied species have a significant biological activity that is probably due to the presence of phenols. To the best of our knowledge, neither detailed phytochemical nor biological studies have been performed for most of the investigated species.

scholarly journals ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM Commiphora leptophloeos (MART.) J. B. GILLETT AGAINST Staphylococcus SPP. ISOLATED FROM CASES OF MASTITIS IN RUMINANTS

2019 ◽  
Vol 20 ◽  
Author(s):  
Isamara Ferreira da Silva ◽  
Amanda Leite Guimarães ◽  
Vinícius Santana Amorim ◽  
Telma Maria Guedes da Silva ◽  
Rodolfo de Moraes Peixoto ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Minimum Bactericidal Concentration ◽  
Therapeutic Potential ◽  
Ethanolic Extract ◽  
High Sensitivity ◽  
Subclinical Mastitis ◽  
Bark Extract ◽  
Ethanolic Extracts ◽  
Staphylococcus Spp

Abstract Considering the therapeutic potential of medicinal plants as alternatives to antibiotic therapy, the research aims to evaluate the antimicrobial activity of Commiphora leptophloeos against isolates of Staphylococcus spp. from the milk of ruminants with subclinical mastitis. For this, the crude ethanolic extract from the bark and leaves of Commiphora leptophloeos was prepared, with these being chemically characterized by HPLC-DAD-MS and by MALDI-TOF. The extracts were then evaluated as to their antimicrobial effects against 60 isolates of Staphylococcus spp. through the broth microdilution technique to determine the minimum bactericidal concentration. In addition, the extracts were evaluated as to their ability to interfere with biofilm formation and with the already established biofilm. Although all tested extracts showed antimicrobial action, lower MBC values were recorded for the bark extract in the concentration 781.2 µg/mL (25/60). The extracts of the bark and leaves were able to interfere with the initial stages of biofilm formation, but there was no interference of the extract on the established biofilm. There was a high sensitivity of Staphylococcus spp. isolates from subclinical mastitis cases in ruminants when subjected to the extracts from bark and leaves of Commiphora leptophloeos, as well as regarding the ability of extracts to interfere in biofilm formation, indicating their potential in the use for ruminant mastitis therapy.

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY ETHANOLIC EXTRACTS OF DRIED FRUITS OF SOLANUM TORVUM (FAMILY-SOLANACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 123
Author(s):  
Lawrence Kalita ◽  
Biswajit Dash ◽  
Uttam Borah ◽  
Juman Deka ◽  
Suvakanta Dash
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Solanum Torvum ◽  
Dried Fruits ◽  
Standard Methods ◽  
Aerial Parts ◽  
Ethanolic Extracts ◽  
Phytochemical Constituents

Objective: To study the antimicrobial activity ethanolic extracts of dried fruits of Solanumtorvum (family-solanaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of dried fruits of the herb Solanumtorvum (family-solanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts were examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of dried fruits of the plant Solanumtorvum (family-solanaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: It can be concluded ethanolic extract of whole aerial parts of the Solanumtorvum (family-solanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Screening of phytochemical compounds, trace metals and antimicrobial activity of Anacyclus pyrethrum

2016 ◽  
Vol 2 (1) ◽  
pp. 32 ◽  
Author(s):  
G. Subasri ◽  
S. Ahmed John
Keyword(s):  
Antimicrobial Activity ◽  
Trace Metals ◽  
Ethanolic Extract ◽  
Reducing Sugar ◽  
Plant Sample ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Phytochemical Compounds ◽  
Positive Results

In the present study is phytochemicals, trace metals and antimicrobial activity of ethanolic extract of Anacyclus pyrethrum. The phytochemical screening of the crude ethanolic root, stem and leaf extracts showed the positive results of steroids, triterpenes, reducing sugar, sugar, alkaloids, flavonoids, saponin, tannins, anthraquinones and amino acids. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn in plant sample were BDL, 0.03, 0.42, 0.79, BDL, BDL and 0.58 mg kg-1, respectively. The descending order of the metal content in the plant sample were: Zn > Fe > Cu > Cd > Cr = Ni = Pb. Among various part of plant extracts studied for antimicrobial activity, root ethanolic extract showed highest of inhibition than leaves and stem ethanolic extracts.

scholarly journals The Influence of Drying Method on Volatile Composition and Sensory Profile of Boletus edulis

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Joanna Nöfer ◽  
Krzysztof Lech ◽  
Adam Figiel ◽  
Antoni Szumny ◽  
Ángel A. Carbonell-Barrachina
Keyword(s):  
Volatile Compounds ◽  
Freeze Drying ◽  
Sensory Profile ◽  
Microwave Drying ◽  
Convective Drying ◽  
Drying Methods ◽  
Drying Method ◽  
Volatile Composition ◽  
Boletus Edulis ◽  
Vacuum Microwave

The objective of this study was to evaluate the influence of different drying methods on aroma and sensory profile of Boletus edulis (cepe). The drying methods tested were convective drying (CD), freeze-drying (FD), vacuum microwave drying (VMD), and a combination of convective predrying and vacuum microwave finish-drying (CPD-VMFD). Fresh and dried cepe volatiles, analyzed by SPME and GC-MS, showed the presence of 53 volatile compounds, most of them present in all dried samples but with quantitative variation. The major volatile compounds in fresh and dried cepe were 1-octen-3-ol (3405 µg 100 g−1·db), 3-octanone (429 µg 100 g−1·db), and hexanal (355 µg 100 g−1·db). The results showed that drying of cepe mushrooms caused major losses of aroma compounds; however, the highest content of volatile compounds and the highest intensity of most of the key positive sensory attributes were found in samples after (i) CD at 80°C (3763 µg 100 g−1·db), (ii) CD at 70°C (3478 µg 100 g−1·db), and (iii) CPD at 60°C and VMFD at 480/240 W (2897 µg 100 g−1·db).

scholarly journals Antioxidant Activity, and Volatile and Phytosterol Contents of Strobilanthes crispus Dehydrated Using Conventional and Vacuum Microwave Drying Methods

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1397 ◽  
Author(s):  
Chua ◽  
Chua ◽  
Figiel ◽  
Chong ◽  
Wojdyło ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Freeze Drying ◽  
Influencing Factor ◽  
Microwave Drying ◽  
Convective Drying ◽  
Drying Methods ◽  
Drying Method ◽  
Vacuum Microwave ◽  
Strobilanthes Crispus ◽  
C 50

The preservation of active constituents in fresh herbs is affected by drying methods. An effective drying method for Strobilanthes crispus which is increasingly marketed as an important herbal tea remains to be reported. This study evaluated the effects of conventional and new drying technologies, namely vacuum microwave drying methods, on the antioxidant activity and yield of essential oil volatiles and phytosterols. These drying methods included convective drying (CD) at 40 °C, 50 °C, and 60 °C; vacuum microwave drying (VMD) at 6, 9, and 12 W/g; convective pre-drying and vacuum microwave finish drying (CPD-VMFD) at 50 °C and 9 W/g; and freeze-drying (FD). GC–MS revealed 33 volatiles, and 2-hexen-1-ol, 2-hexenal, 1-octen-3-ol, linalool, and benzaldehyde were major constituents. The compounds β-sitosterol and α-linolenic acid were the most abundant phytosterol and fatty acid, respectively, in fresh S. crispus. The highest phenolic content was achieved with CD at 60 °C. The highest antioxidant activity was obtained with CD at 40 °C and VMD at 9 W/g. On the contrary, the highest total volatiles and phytosterols were detected with CD at 50 °C and VMD at 9 W/g, respectively. This study showed that CD and VMD were effective in producing highly bioactive S. crispus. A suitable drying parameter level, irrespective of the drying method used, was an important influencing factor.

scholarly journals Effects of Various Drying Methods on Some Physico-Chemical Properties and the Antioxidant Profile and ACE Inhibition Activity of Oyster Mushrooms (Pleurotus Ostreatus)

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 160 ◽  
Author(s):  
Sergey Piskov ◽  
Lyudmila Timchenko ◽  
Wolf-Dieter Grimm ◽  
Igor Rzhepakovsky ◽  
Svetlana Avanesyan ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Pleurotus Ostreatus ◽  
Microwave Drying ◽  
Drying Methods ◽  
The Sun ◽  
Ace Inhibitory Activity ◽  
Peptide Profile ◽  
Hot Air ◽  
Ace Inhibitory

In food biotechnology, Pleurotus ostreatus is of great interest as a source of natural antioxidants and angiotensin-converting enzyme (ACE) inhibitors. However, research in this area has not yet been completed. The effect of various drying methods on the structural properties and the rehydration capacity of mushrooms was investigated in this paper. The content of secondary metabolites, the peptide profile, and the antioxidative effect and ACE inhibitory activity of dry mushrooms were investigated in vitro, simulating the process of gastrointestinal digestion. X-ray microtomography has confirmed that structure of lyophilic and sun-dried mushrooms is dominated by open pores, and in mushrooms dried with hot air and microwave, closed pores. Experiments have shown that the conditions of freeze drying and sun drying of Pleurotus ostreatus provide a higher rehydration capacity of dried mushrooms. The maximum activity of radical absorption of the oyster mushroom after microwave drying was observed. The iron restoring capacity of the mushrooms is maximally maintained with microwave drying and hot-air drying. The properties of the antioxidant product with an emphasis on the high activity of inhibiting lipid oxidation of the mushroom maximized after drying in the sun. Mushrooms dried lyophilically and in the sun showed the highest ACE inhibitory activity.

scholarly journals Phytochemical Screening, Pharmacognostic Evaluation and Biological Activity of Amaranthus spinosus L

2015 ◽  
Vol 1 (4) ◽  
pp. 29-34
Author(s):  
Dharma Prasad Khanal ◽  
B Raut ◽  
K S Dangol
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Rural Areas ◽  
Herbal Medicines ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Amaranthus Spinosus ◽  
Aerial Parts ◽  
Safety Margins ◽  
Phytochemical Constituents

Herbal medicines possess a great demand in both developed and developing countries as a source of primary health care owing to their attributes having wide biological and medicinal activities, high safety margins and lesser costs. People living in rural areas of Nepal depend largely in the herbal medicines for the treatment. The present study concerns with the study of pharmacognostic characteristics, phytochemical constituents and the biological activity of Amaranthus spinosus L. The hexane, chloroform, ethanolic and aqueous extract of its aerial parts were subjected to preliminary phytochemical analysis and detected saponin, carbohydrate, tannin, protein, glycoside, flavonoid and phenol as phytoconstituents. The transverse section of the stem and leaves showed characteristic vascular bundle tissues. All the extracts were used for testing the antimicrobial activity and brine shrimp lethality. No extract showed the antimicrobial activity and ethanolic extract showed LC50 value of 31.62 ppm. Hexane, chloroform and water extracts were less toxic with LC50 values 236.88 ppm,194.98 ppm and 320.71 ppm respectively.DOI: http://dx.doi.org/10.3126/jmmihs.v1i4.11999Journal of Manmohan Memorial Institute of Health Sciences Vol. 1, Issue 4, 2015Page: 29-34

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