scholarly journals The anticancer activity of ethanol extract of Chromolaena odorata leaves in 7,12-Dimethylbenz[a]anthracene in (DMBA) induced breast cancer Wistar rats (Rattus novergicus)

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 493-499
Author(s):  
Hanifah Yusuf ◽  
Reno Keumalazia Kamarlis ◽  
Yusni Yusni ◽  
Marhami Fahriani
Keyword(s):  
Breast Cancer ◽  
Body Weight ◽  
Treatment Group ◽  
Anticancer Activity ◽  
Cancer Control ◽  
Ethanol Extract ◽  
Breast Cancer Chemotherapy ◽  
Treatment Groups ◽  
Post Hoc ◽  
Dose Dependent

Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardiotoxicity. Therefore, this study aims to evaluate the anticancer activity of C. odorata leaves extract in DMBA induced breast cancer on rats. Methods: Seven groups of Rattus novergicus were used: Four treatment groups of C. odorata extract (500, 1000, 2000, and 4000 mg/kg BW), normal control, breast cancer control, and doxorubicin treatment group. The number, volume, and weight of the nodule and the rats’ body weight were compared among groups. Data was analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate. Results: Significant decline of the number, volume, and weight of cancer nodules was observed in the treatment group (p < 0.001). The weight of the cancer nodule at week 16th was also significantly reduced in GCo2000 compared to Gdoxo (p < 0.0001). A significant increase in body weight was also dose-dependent, especially at week 11th (p < 0.05 in all comparisons) and week 16th (p < 0.001 in all comparisons). Conclusion: This study suggested that the ethanol extract of C. odorata leaves has anticancer and antiproliferative activity.

scholarly journals MORTALITAS DAN RESORPSI FETUS MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK ETANOL TANAMAN SURUHAN (Peperomia pellucida (L.,) Kunth.)

2021 ◽  
pp. 194-202
Author(s):  
Hendri Busman ◽  
Nuning Nurcahyani ◽  
Yosi Dwi Saputra ◽  
Salman Farisi ◽  
Qotrunnada Salsabila
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Medicinal Use ◽  
One Way Anova ◽  
Different Doses

Tanaman Suruhan (Peperomia pellucida (L.,) Kunth.) merupakan tanaman obat yang memiliki senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, dan steroid. Berbagai senyawa kimia tersebut yang berpotensi sebagai obat tetapi perlu diperhatikan kemungkinan adanya efek samping terhadap organisme khususnya pada masa kehamilan. Penelitian ini bertujuan menguji efek teratogenik ekstrak tanaman suruhan terhadap fetus mencit (Mus musculus L.), meliputi mortalitas dan resorpsi. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan  20 ekor mencit betina dibagi ke dalam 4 kelompok yaitu: K(+) (Aquabides), P1 diberi ekstrak tanaman suruhan dengan dosis (1,68 mg/g bb), P2 (3,36 mg/g bb), dan P3 (6,72 mg/g bb). Hasil penelitian terhadap persentase fetus yang mengalami mortalitas dan resorpsi fetus antara kontrol K(+) dan perlakuan dengan ekstrak etanol suruhan (P1, P2, dan P3) tidak menunjukkan perbedaan yang bermakna secara statistik berdasarkan uji ANOVA satu faktor (p value 0,418). Hasil penelitian yang didapat menunjukkan bahwa ekstrak etanol tanaman suruhan tidak menyebabkan mortalitas pada fetus mencit, namun menyebabkan resorpsi pada fetus mencit pada pemberian dosis sebesar 1,62 mg/g BB, 3,36 mg/g BB, dan 6,72 mg/g BB.   Pepper elder (Peperomia pellucida (L.,) Kunth.) is a medicinal plant that has secondary metabolites such as alkaloids, flavonoids, saponins, tannins, and steroids. The side effects for organisms of those chemical compounds, which are potentially beneficial for their medicinal use, still need to be considered especially in pregnancy. This study aims to determine the teratogenic effects of pepper elder extract on mortality and resorption of mice (Mus musculus L.) fetus. This study uses total random sampling design with 4 treatments consisting of 20 female mice divided into 4 groups: the control group (K(+)) which is given Aqua distillation and the treatment groups which are all given the pepper elder extract with different doses, where the first treatment group (P1) is given 1.68 mg/g body weight of the extract, the second treatment group (P2) is given 3.36 mg/g body weight of the extract, and the third treatment group (P3) is given 6.72 mg/g body weight of the extract. The results obtained of mortality and resorption percentage of mice fetus between the control group (K(+)) and the treatment groups (P1, P2, and P3) showed that there is no statistically meaningful difference based on one-way ANOVA test (p value 0,418). This study showed that ethanol extract of pepper elder causes no mortality in mice fetus, yet it causes resorption on mice fetus at given doses of 1,62 mg/g body weight, 3,36 mg/g body weight, dan 6,72 mg/g body weight.

Evaluation of unconventional poultry feed in broilers

2019 ◽  
Vol 17 (1) ◽  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Blood Meal ◽  
Feed Consumption ◽  
Trial Period ◽  
Feed Ingredients ◽  
Treatment Groups ◽  
Broiler Production ◽  
Group I ◽  
Unconventional Feed

Background: Commercial broiler is a rapidly growing sector in Bangladesh. The broilers are supplied with the balanced ration prepared from a number of ingredients which are not available uniformly throughout the years. On the other hands, manufactured feeds are costly and inconvenient for the rural farmers. This study is undertaken to use unconventional feed ingredients aiming to decrease the broiler production cost. Method: A total of ninety-nine day- old broiler chicks (Hubbard) of either sex were used in this study in 3 treatment groups to assess processed unconventional feed item (shoti, blood meal, poultry dropping and molasses) on growth and profitability upon rearing for 60 days. Broilers were reared in dip litter system and were fed ad libitum on conventional and unconventional diet dividing into 3 treatment diets namely, treatment diet 1 (T1: Conventional as control), treatment diet 2 (T2: unconventional with blood meal and shoti), and treatment diet 3 (T3: unconventional with blood meal, shoti, poultry droppings and molasses) throughout the trial period. Treatment group 1 (T1) was used as control. Results: Broilers fed on control diet (conventional feed) achieved higher (P<0.01) body weight, while the broilers offered diets with the highest amount of unconventional feed had the lowest body weight. Increased (P<0.01) feed intake was observed during 21days and 49 days of age when broilers fed diets with the supplementation, but no significant differences were found among the groups in terms of feed consumption during 33 day of age. FCR differed significantly (P<.01) throughout the trial period with the broilers fed diets without supplementation of unconventional feed item had the superior FCR than the others. Mortality rate was 6.06%,9.09%, and 9.09% in treatment group I, treatment group II, and treatment group III respectively while live weights, feed conversion and feed consumption was unaffected by all the dietary groups with/without incorporation of unconventional feed item up to day 60 days of age. Statistically significant (P<0.01) decreased live weight was observed among the treatment groups in 60-day-old birds. Productivity and cost-benefit analysis were performed. Conclusion: Use of unconventional feed ingredients in broiler production greatly reduces the feed cost with little hampering the growth rate and hence, it is profitable for the farmers.

scholarly journals Total Phenols, Identification of Active Compounds and Anticancer Activity of Salvia judaica Boiss against the breast Cancer Cell MDA-231

2021 ◽  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Essential Oil ◽  
Anticancer Activity ◽  
Breast Cancer Cell ◽  
Annexin V ◽  
Ethanol Extract ◽  
G1 Phase ◽  
Apoptotic Cells ◽  
Total Phenols

Salvia judaica is an annual herb from genus Salvia L.; the largest genera of Lamiaceae. It’s a medicinal plant prominent in pharmaceutical applications in many countries around the world. This study aimed to explore bioactive compounds likely to be responsible for the plant anticancer activity, and evaluate anticancer effects, after determining the total content of phenols in the ethanol extract and essential oil in this species. Ethanol extract (EE) and essential oil (EO) were prepared from dried aerial parts (leaves and the flower). GC-MS analysis of EO showed the presence of/43/ effective compounds in varying proportions, the major compounds were sesquiterpenes like delta-cadinene, alpha-Gurjunene, beta-humulene, and alpha-caryophyllene. This is the first study revealed that S.judaica is so rich in phenols which proceeded S.officinalis, noting the superiority of the EE over the EO samples in the total phenols. Anticancer properties of EE and EO of S. judaica against MDA-231 breast cancer cell line were studied -for the first time - by cell cycle analysis and Annexin V/PI apoptosis assay using Flow cytometry technique. Cells were treated with EE (0.001, 0.01, 0.02, 0.1mg/ml) and EO (0.005, 0.01, 0.02, 0.03, 0.04 mg/ml) at various concentrations for48 h. The results revealed that both EE and EO induced cell cycle arrest at G1-phase. Cells treated with EE and EO for 48h showed increasing the percentage of cells in G1-phase and decreasing the percentage of cells in S-phase with increasing concentration compared with untreated cells (control). Annexin V-FITC/PI assay confirmed that EO and EE were able to induce apoptosis. Cells treated with EOat (0.04 mg/ml) for 48h resulted in apoptotic cells at 96.68%, and necrotic cells at 0.12%, compared with untreated cells. On the other hand, Cells treated with EE at (0.1 mg/ml) for 48h resulted in apoptotic cells at 94.43%, and necrotic cells at 0.47%, compared with control. Results revealed that EO is better than EE as anticancer; treatment with EO resulted in more apoptotic cells and less necrotic cells, and there were significant differences between them. This confirmed that EO contains specific anticancer compounds as showed by GC-MS analysis. However, more studies should be performed to explore antioxidants present in S.judaica and determine the underlying mechanism of their anti-breast cancer properties.

scholarly journals Biochemical, Histopathological and Mutagenic Changes Following the Co-administration of Antihelminthic and Antimalarial Drugs in Wistar Rats

2019 ◽  
pp. 1-12
Author(s):  
A. A. Obiajunwa ◽  
E. T. Idowu ◽  
O. A. Otubanjo
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Fatty Infiltration ◽  
Portal Tract ◽  
Renal Tissue ◽  
Albino Rats ◽  
Male Adult ◽  
Treatment Groups ◽  
Combination Treatments ◽  
Therapeutic Doses

Aim: To determine the effects of antimalaria and antheminthic drugs combination in the incidence of histopathological alteration and biochemical modulations in liver and kidney of albino rats. Place and Duration of Study: The study was undertaken at the Zoology Department University of Lagos Akoka Lagos Nigeria. Methodology: A total of twenty (25) Male adult albino rats of 13-15 weeks old were divided into 5 groups of 5 rats each and daily oral administration of human therapeutic doses of praziquantel (PZQ 50 mg/kg body weight) separate and in combination with ivermectin (IVM 0.4 mg/kg body weight), albendazole (ALB 15 mg/kg body weight) and Artemether-lumefanthrine (ACT 140 mg/kg body weight) was administered with the  group which serve as the control receiving 1ml distilled water. Toxic effects due to these treatments were investigated using histopathological, biochemical and mutagenic indices at day 8th and 15th of the study. Results: Biochemical assessment revealed significant reduction in AST, ALT, ALP and potassium in the treatment group compared to the control. Increase in the level calcium, Albumin and bicarbonate were also observed in treatment groups. Histopathological assessment of the liver showed a general incidence of focal inflammation along the portal tract area, but did not show any differential severity across treatment groups except for single PZQ treatment group which were characterized by fatty infiltration. A general occurrence of mesangial damage and glomerula injury was observed in kidney tissues. Renal lesions were more severe in single PZQ + IVM treatment groups while mild lesions characterized renal tissue from PZQ+ACT treatment groups. Mutagenic effects as indicated by the high incidence of sperm head abnormalities was recorded across combination treatments especially in PZQ+ IVR and PZQ+ ACT groups. Conclusion: Findings suggest that combination therapies are synergistic and could result in nephrotoxicity, antidiuretic effects, dehydration and mutagenicity at human therapeutic doses.

scholarly journals Prebiotics Fructo-, Galacto-, and Mannan-Oligosaccharide Do Not Protect against 5-Fluorouracil–Induced Intestinal Mucositis in Rats

2019 ◽  
Vol 149 (12) ◽  
pp. 2164-2173 ◽  
Author(s):  
Roger Yazbeck ◽  
Ruth J Lindsay ◽  
Mark S Geier ◽  
Ross N Butler ◽  
Gordon S Howarth
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Myeloperoxidase Activity ◽  
Intestinal Damage ◽  
Small Intestinal Mucosa ◽  
Villus Height ◽  
Mannan Oligosaccharide ◽  
Treatment Groups ◽  
Small Intestinal ◽  
All Treatment

ABSTRACT Background Prebiotics selectively stimulate the growth of beneficial bacteria within the gastrointestinal tract, and have been investigated in human and animal studies for their capacity to improve intestinal health. Objective We investigated the prebiotics fructo-oligosaccharide (FOS), galacto-oligosaccharide (GOS), and mannan-oligosaccharide (MOS) for their potential to alleviate intestinal damage in rats. Methods Female Dark Agouti rats (6–8 wk old, 110–150 g) were allocated to 1 of the following treatment groups (n = 8/group): saline/water, saline/FOS, saline/GOS, saline/MOS, 5-fluorouracil (5FU)/water, 5FU/FOS, 5FU/GOS, and 5FU/MOS. Rats were pretreated with either 5% GOS, MOS, or FOS or vehicle (water) from day −12 to day 0. On day 0, rats received a single intraperitoneal injection of saline or 5FU. Metabolic data were recorded daily and all rats were killed on day 3. Histopathology was quantified in hematoxylin and eosin–stained sections. Intestinal sucrase and myeloperoxidase activity were quantified by biochemical assay. Fecal SCFAs—acetic, propionic, and butyric acid—were also measured. Statistical analysis was by repeated-measures, 2-factor ANOVA or Kruskal–Wallis and Mann–Whitney U test; P < 0.05 was considered statistically significant. Results Body weight was significantly decreased in all treatment groups after 5FU injection, with no change in body weight observed in any prebiotic treatment group. Total food intake was lower by ≥7% in the GOS treatment group pre-5FU than in all other groups (P < 0.05). Ileal villus height was 18% higher in GOS-treated rats pre-5FU than in respective water controls (P < 0.05). Jejunal and ileal villus height and crypt depth were significantly decreased in all treatment groups after 5FU injection, with no prebiotic effect observed. SCFAs were differentially increased in prebiotic treatment groups compared with water-only controls (P < 0.05). Conclusions FOS, GOS, and MOS have differential effects in modifying small intestinal pathology and SCFA profiles in rats with healthy and damaged small intestinal mucosa.

scholarly journals In VivoAntiplasmodial and Analgesic Effect of Crude Ethanol Extract ofPiper guineenseLeaf Extract inAlbinoMice

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
A. Y. Kabiru ◽  
G. F. Ibikunle ◽  
D. A. Innalegwu ◽  
B. M. Bola ◽  
F. M. Madaki
Keyword(s):  
Body Weight ◽  
Analgesic Efficacy ◽  
Ethanol Extract ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Piper Guineense ◽  
Standard Drug ◽  
Analgesic Effects ◽  
Dose Dependent

Antiplasmodial and analgesic effects of crude ethanol extract ofPiper guineensewas investigated in mice. The antiplasmodial and analgesic efficacy of the extract was judged on its ability to reduce parasitemia and writhing, respectively, in mice. The antiplasmodial screening involved treating infected mice with 200, 400, and 600 mg/kg body weight of extract while the positive control group was given standard artesunate drug. The analgesic test was carried out by administering 1000, 1500, and 2000 mg/kg body weight of extract to three groups of healthy mice, respectively, after induction of pain with 0.75% acetic acid. The positive control group was given aspirin drug. Parasitemia was reduced by 28.36%, 43.28%, and 62.69% in a dose-dependent pattern in the curative test which was significantly different (P<0.05) from 96.03% of the standard drug. The reduction of writhing by mice given the extract was also dose-dependent (36.29, 45.43, and 59.07%). Aspirin drug was however more effective (86.36%). The extract was safe at 2000 mg/kg body weight. Phytochemical screening revealed the presence of flavonoids, tannins, phlobatannins, terpenoids, and coumarins. Result obtained in this study demonstrated the efficacy of ethanol extract ofPiper guineenseas an antiplasmodial and analgesic agent.

scholarly journals Reproductive Toxicities Caused by Swainsonine from Locoweed in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Chenchen Wu ◽  
Ke Feng ◽  
Dezhang Lu ◽  
Dujian Yan ◽  
Tiesuo Han ◽  
...  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Intraperitoneal Injection ◽  
First Generation ◽  
Lower Body ◽  
Estrous Cycles ◽  
Treatment Groups ◽  
Three Generations ◽  
Body Weights ◽  
Liver And Kidney

Swainsonine is the primary toxin in locoweeds. It causes intention tremors, reproductive dysfunction, emaciation, and death. The objective of the present study was to evaluate the potential reproductive and developmental toxicities caused by swainsonine in mice. The treatment groups consisting of three generations of mice were given a range of concentrations of swainsonine by intraperitoneal injection (2.50 mg/kg body weight (BW), 1.20 mg/kg BW, 0.60 mg/kg BW, and 0 mg/kg BW). The 0 mg/kg BW group exhibited significantly fewer estrous cycles and an increased number of estrous ones compared to the 2.50 mg/kg BW, 1.20 mg/kg BW, and 0.60 mg/kg BW groups (P<0.05). All three generations of mice treated with swainsonine had significantly higher spleen, liver, and kidney indices and significantly lower body weights compared to the 0 mg/kg BW group (P<0.05). For the first and second generations of treatment group, the copulation indices and the numbers of live pups on postnatal days (PND) 0, 4, and 15 were significantly decreased compared to those of the 0 mg/kg BW group (P<0.05). The fertility and gestation indices of the treatment group of the first generation were significantly increased compared to the 2.50 mg/kg BW, 1.20 mg/kg BW, and 0.60 mg/kg BW groups of the second generation (P<0.05). Cumulatively, these results indicate that swainsonine may cause reproductive and developmental toxicities in mice in both parents and offspring.

scholarly journals Ameliorative Effects of Ascorbic Acid and Allium sativum (Garlic) Ethanol Extract on Renal Parenchyma of Gentamicin-induced Nephropathic Rats

2020 ◽  
pp. 1-8
Author(s):  
Dayo Rotimi Omotoso ◽  
Joy Motunrayo Olajumoke
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Allium Sativum ◽  
Combined Treatment ◽  
Ethanol Extract ◽  
Renal Tissue ◽  
Renal Parenchyma ◽  
Histopathological Changes ◽  
Treatment Groups ◽  
Tissue Weight

To assess ameliorative effects of Ascorbic acid (AA) and Allium sativum ethanol extract (ASEE) on renal parenchyma of gentamicin-induced nephropathic rats. Thirty Wistar rats (weighing between 180-205 g) were randomly divided into five groups (A-E).  These include Group A administered with 0.9% Normal Saline (0.5 ml/kg body weight (b.w.)), Group B administered with gentamicin (GM, 200 mg/kg b.w.) intraperitoneally (i.p.), Group C administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally, Group D administered with GM (200 mg/kg b.w.) i.p. and ASEE (200 mg/kg b.w.) orally and Group E administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally and ASEE (200 mg/kg b.w.) orally. All administrations were done once daily for a period of ten (10) days. The body weight of study animals was recorded at the beginning and end of study period. After the study period, renal tissue of study animals was harvested, weighed, processed, stained using H & E technique. Stained sections were examined under microscope for histopathological changes within the renal parenchyma and were scored using image-J software. The results of this study showed that exposure to GM results into significant (P < 0.05) reduction in body and renal tissue weight. However, therapeutic exposure to AA and ASEE either as individual or combined treatment regimen culminated into relatively null body and renal tissue weight loss among treatment groups C-E. In addition, exposure to GM precipitates prominent histopathological changes within renal parenchyma of study animals.  As observed with body and renal tissue weight changes, treatment with AA and ASEE also comparatively ameliorate GM-induced nephropathy within renal parenchyma of study animals in treatment groups. The findings of this study therefore showed that AA and ASEE exhibit ameliorative effect on the renal parenchyma of gentamicin-induced nephropathic rats either as distinct or combined treatment regimen.

scholarly journals Response and Economic Efficiency of Broiler Chickens fed graded levels of Baker's Yeast (Saccharomyces cerevisiae) supplement

2021 ◽  
Vol 43 (2) ◽  
pp. 304-313
Author(s):  
W. Buba ◽  
T. S. Olugbemi ◽  
J. J Omage ◽  
S. Duru ◽  
A. O Iyiola-Tunji
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Body Weight ◽  
Weight Gain ◽  
Treatment Group ◽  
Economic Efficiency ◽  
Broiler Chickens ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Treatment Groups

Two hundred and fifty-five day-old Ross White strain broiler chicks were used to evaluate the Response and economic efficiency of broiler chickens fed graded levels of baker's yeast (saccharomyces cerevisiae) supplement during the harmattan season in the northern guinea savannah zone of Nigeria. There were five treatment groups for both starter and finisher phases. The experiment lasted for eight weeks. Each treatment group had three replicates with 17 birds per replicate and fifty-five birds per group in a completely randomized design (CRD). Treatments 1, 2, 3, 4 and 5 contained 0.0, 0.5, 1.0, 1.5 and 2.0% SC respectively. Results of the starter phase showed significant (P<0.05) differences in terms of final weight gain and feed conversion ratio (FCR). Treatment groups fed the control diet (0.0%), 0.5% and 1% SC had the best final body weight and body weight gain during the starter phase. The FCR was significantly (P<0.05) higher in the treatment group fed 0.5% SC during the starter phase. Mortality was higher (11.76%) in the control group compared to other treatment groups in the starter phase. During the finisher phase, treatment group fed 1.0% SC had significantly (P<0.05) higher final body weight, body weight gain and FCR. Apparent digestibility of crude protein, crude fibre, ether extract, ash, dry matter and nitrogen free extract were better in treatment groups containing SC. The economic efficiency results of this study indicated that the birds fed 1.0% SC were more profitable compared to those fed the control diet. The net profit of the treatment group fed 1.0% SC was N 650.01 compared to the control (N554.35). This study concluded that the optimum inclusion level of Baker's yeast (Saccharomyces cerevisiae) in the diet of broilers starter was 0.5% and 1.0% for finisher diet for improved performance in terms of weight gain and feed conversion ratio (FCR). It is suggested that broiler chicken farmers should be encouraged to include Baker's yeast in the diet of broiler chickens for improved weight productivity and better feed utilization.

Cytotoxicity Activity of Ethanol Extract of Andaliman Fruits (Zanthoxylum acanthopodium DC.) towards 4T1 Breast Cancer Cells

2019 ◽  
Vol 2 (2) ◽  
pp. 31-35
Author(s):  
Rosidah Rosidah ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Ginda Haro ◽  
Denny Satria
Keyword(s):  
Breast Cancer ◽  
Natural Products ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Chemotherapeutic Agents ◽  
Cytotoxicity Activity ◽  
4T1 Cells ◽  
4T1 Breast Cancer

Breast cancer is one of the world's leading cause of death in women. Due to the resistance of chemotherapeutic agents, there is a continuous need to search of natural products with anticancer activity.  The use of natural products  is expected to increase the effectiveness  and decrease side effect. The purpose of this study was to investigate the anticancer activity of ethanol extract of andaliman fruits (EEAF) towards 4T1 cells. Extracts were prepared by maceration using solvent ethanol 96%. 4T1 cells were grown in culture medium DMEM then given by EEAF and doxorubicin. Cytotoxic test in vitro was done by MTT method [3-(4,5-dimetiltiazol-2-il) -2.5 difeniltetrazolium bromide] which is then analyzed using SPSS 21. The results from this study showed that the cytotoxic results (IC50) after treatment with EEAF and doxorubicin were 54.48 ± 0.22 µg/mL dan 0.80 ± 0.02 µg/mL.Based on the result above, we conclude that EEAF has cytotoxic activity towards 4T1 cancer cells. Key words: andaliman fruits, Zanthoxylum acanthopodium DC., ethanol extract, breast cancer, 4T1 cell line.

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