Formulation and evaluation of the nanosilver gel using bioreductor ethanol extract of katuk leaf (sauropus androgynus (l) merr) as antibacterial

Background: The current type of nanoparticle that attracts a lot of attention in the pharmaceutical field is Nanosilver because it can act as an antimicrobial. Objective: The aim of this study was to obtain the Nanosilver gel preparation from katuk leaf extract, which is effective as an antibacterial agent against acne. Methods: Katuk leaf extract remains as a reducing agent in the synthesis of Nanosilver using a green synthesis method. Nanosilver characterization was performed using PSA, FTIR and TEM. Results: Particle size of Nanosilver (Katuk leaf extract concentration 0.16% with a silver nitrate volume of 10 mM is 1: 9) of 164.40 nm, Nanosilver (Katuk leaf extract Concentration 0.32% with silver nitrate volume 10 mM is 1: 9) of 176.47 nm and Nanosilver (0.48% katuk leaf extract concentration with a volume of 10 mM silver nitrate of 1: 9) of 194.95 nm. Antibacterial activity using the Well method was shown that the nanosilver gel from Katuk leaf extract had an inhibitory effect on P. acnes bacteria with an inhibition zone of 26 mm classified as very strong, and 12 mm was strongly categorized against S. aureus bacteria, namely in Formula 1. Conclusion: A variation in the concentration of katuk leaf extract influences the size of the particles formed. the Nanosilver katuk leaf extract has an antibacterial cause for acne with a very strong category.

Download Full-text

The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth

JURNAL ILMIAH PLATAX ◽

10.35800/jip.2.2.2014.7147 ◽

2014 ◽

Vol 2 (2) ◽

pp. 40

Author(s):

Galih Arif Kusuma ◽

Sammy N. J. Longdong ◽

Reiny A. Tumbol

Keyword(s):

Antibacterial Activity ◽

Aeromonas Hydrophila ◽

Leaf Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Bacteria Growth ◽

Activity Test ◽

Inhibitory Power

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.

Download Full-text

Uji daya hambat ekstrak daun patikan kerbau (euphorbia hirta l.) terhadap pertumbuhan bakteri staphylococcus aureus dan escherichia coli

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.14654 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Florencia I. Mahmud ◽

Christi Mambo ◽

Henoch Awaloei

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Leaf Extract ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Euphorbia Hirta ◽

E Coli ◽

Extract Concentration

Abstract: Patikan kerbau leaf contains alkaloid, flavonoid, phenol, and tannin can potentially be an antibacterial. The purpose of this research is to test the resisting potency of patikan kerbau leaf extract against Staphylococcus aureus (ATCC25923) dan Escherichia coli (ATCC11229). This was an experimental laboratory study using modified Kirby-Bauer with well diffusion technique at Research and Microbiology Laboratory of MIPA Faculty, Sam Ratulangi University Manado. Patikan kerbau leaf extract was obtained by using 96% etanol maceration. Extract concentrations used in this study were 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml. Ciprofloxacin was used as the positive control and CMC as the negative control. The CMC showed no inhibition zone. Ciprofloxacin had the widest zone of inhibition. The average of inhibition zone diameters produced by ciprofloxacin was 33,3 mm on S. aureus and 33 mm on E.coli. Euphorbia hirta leaf extract concentration of 50 mg/ml resulted in average inhibition zone diameter of 18.83 mm on S.aureus and 17.83 mm on E.coli. Extract concentration of 25 mg/ml resulted in 17.33 mm on S. aureus and 16.83 mm on E.coli. Extract concentration of 12,5 mg/ml resulted in 15.5 mm on S. aureus and 14.83 mm on E.coli. Then, extract concentration of 6.25 mg/ml resulted in 15.16 mm on S. aureus and 13.3 mm on E.coli. Conclusion: Extract of Euphorbia hirta leaf has potential inhibitory effect towards bacterial growth of S. aureus and E. coli. Moreover, the inhibitory effect of Euphorbia hirta extract is greater towards S. aureus rather than E.coliKeywords: antibacterial, patikan kerbau leaf extract, S. aureus, E. coli Abstrak: Daun patikan kerbau mengandung alkaloid, flavonoid, fenol dan tanin yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui uji daya hambat ekstrak daun patikan kerbau terhadap Staphylococcus aureus (ATCC25923) dan Escherichia coli (ATCC11229). Jenis penelitian ini ialah eksperimental laboratorium di Laboratorium Penelitian dan Mikrobiologi Fakultas MIPA Universitas Sam Ratulangi Manado dengan metode Kirby-Bauer yang dimodifikasi dengan sumuran. Ekstrak daun patikan kerbau diperoleh dari proses maserasi dengan etanol 96%. Konsentrasi ekstrak yang digunakan dalam penelitian ialah 50mg/ml, 25mg/ml, 12,5mg/ml dan 6,25mg/ml. Siprofloksasin digunakan sebagai kontrol positif dan CMC sebagai kontrol negatif. Pada penelitian ini CMC yang tidak mempunyai zona hambat. Siprofloksasin memiliki diameter zona hambat yang paling besar. Rerata diameter zona hambat yang dihasilkan oleh siprofloksasin adalah 33,3 mm terhadap bakteri S.aureus dan 33 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 50 mg/ml menghasilkan diameter zona hambat rata-rata sebesar 18,83 mm pada bakteri S.aureus dan 17,83 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 25mg/ml sebesar 17,3 mm pada bakteri S.aureus dan 16,83 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 12,5mg/ml sebesar 15,5 mm pada bakteri S.aureus dan 14,83 mm pada bakteri E.coli. Kemudian, konsentrasi 6,25mg/ml sebesar 15,16 pada bakteri S.aureus dan 13,3 mm pada bakteri E.coli. Simpulan: Ekstrak daun patikan kerbau berpotensi memiliki efek daya hambat terhadap pertumbuhan bakteri S.aureus dan E.coli. Daya hambat ekstrak daun patikan kerbau lebih besar pada S.aureus daripada E.coli Kata kunci: antibakteri, ekstrak daun srikaya, S. aureus, E. coli

Download Full-text

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

Interdental Jurnal Kedokteran Gigi (IJKG) ◽

10.46862/interdental.v17i2.2400 ◽

2021 ◽

Vol 17 (2) ◽

pp. 89-96

Author(s):

Ratih Widyasari ◽

◽

Willy Hadinata Halim ◽

Atia Nurul Sidiqa ◽

Dewa Made Wedagama ◽

...

Keyword(s):

Leaf Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Endodontic Treatment ◽

Leaf Extracts ◽

Herbal Products ◽

Inhibition Zone Diameter ◽

Zone Diameter ◽

The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

Download Full-text

Potensi Daun Teh (Camellia sinensis) dan Daun Anting-anting Acalypha indica L. dalam Menghambat Pertumbuhan Salmonella typhi

JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI ◽

10.36722/sst.v2i2.131 ◽

2013 ◽

Vol 2 (2) ◽

pp. 104

Author(s):

Nita Noriko

Keyword(s):

Camellia Sinensis ◽

Antibacterial Agent ◽

Leaf Extract ◽

Bacterial Resistance ◽

Inhibition Zone ◽

Salmonella Typhi ◽

The Body ◽

Microbiology Laboratory ◽

Clear Zone ◽

Acalypha Indica

Abstrak - Salmonella typhi adalah bakteri penyebab terjadinya penyakit typhus. Pengobatan menggunakan antibiotika menimbulkan resistensi jika dilakukan tidak tuntas terutama ketika sistem tubuh menurun. Pengobatan dengan menerapkan kearifan lokal merupakan salah satu alternatif dalam upaya penyembuhan. Daun teh (Camellia sinensis) dan daun anting-anting (Acalypha indica L) diduga mengandung tanin, suatu senyawa yang dapat berfungsi sebagai antibiotik. Tujuan dari penelitian ini adalah memperoleh informasi efektifitas ekstrak daun teh dan daun anting-anting sebagai antibakteri alami, khususnya S. typhi Penelitian dilakukan pada bulan Maret hingga Oktober 2012 di Laboratorium Mikrobiologi Universitas Al Azhar Indonesia. Tahapan penelitian terdiri dari pembuatan ekstrak daun teh dan anting-anting, pengujian adanya kandungan tanin, penumbuhan S. typhi dan uji antibakteri. Ekstrak daun diperoleh dengan cara perebusan dan pengeringan. Hasil penelitian menunjukkan bahwa ekstrak daun teh dan anting-anting berpotensi untuk dikembangkan sebagai antibiotik. Hal ini ditunjukkan dengan terbentuknya zona bening ketika dilakukan uji antibakteri. Kedua ekstrak daun tersebut positif mengandung tanin yang diduga berperan dalam penghambatan pertumbuhan S. typhi. Abstract - Salmonella typhi is a bacteria that caused typhus disease. The curation using chemical drugs may cause bacterial resistance if the treatment is not complete, especially when the system of the body decreases. Treatment with traditional medicine is one of alternative way for curing typhoid fever. Tea and Anting-anting leaves are suspected to contain tannin, a compound that can act as an antibiotic. The aim of the research is getting information about effectiveness of tea and anting-anting leaf extract as antibacterial agent specially S. thypi. Research were conducted from Maret until October 2012 in Microbiology Laboratory, Al Azhar Indonesia University. The steps incude collecting tea and anting-anting leaves, extracting tannin, testing for tannin, growing the bacteria, and testing the inhibition zone. Leaf extract obtained by boiling and drying. The research shows that leaf tea and anting-anting extraction have potential to be developed as an antibiotic. That are showed by clear zone as indicate inhibition Salmonella typhi when tested antibacterial. Both of the leaves plant are positive tannin containing, and it is indicated as inhibitor S. thypi growth.

Download Full-text

EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v2i2.84 ◽

2020 ◽

Vol 2 (2) ◽

pp. 105-112

Author(s):

Irene Puspa Dewi ◽

Ilham Maslan Orde ◽

Verawaty Verawaty

Keyword(s):

Staphylococcus Aureus ◽

Allium Sativum ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Traditional Medicines ◽

Significant Difference ◽

Irritation Test ◽

Gel Preparation ◽

The One

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

Download Full-text

Uji Aktivitas Ekstrak Etanolik Daun Kemangi (Ocimum sanctum L.) terhadap Staphylococcus aureus secara In Vitr

Jurnal Pharmascience ◽

10.20527/jps.v7i1.8080 ◽

2020 ◽

Vol 7 (1) ◽

pp. 107

Author(s):

Novia Ariani ◽

Dwi Rizki Febrianti ◽

Rakhmadhan Niah

Keyword(s):

Staphylococcus Aureus ◽

Sampling Technique ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Ocimum Sanctum ◽

Purposive Sampling ◽

Extract Concentration

ABSTRAK Tanaman kemangi banyak dimanfaatkan oleh masyarakat untuk pengobatan infeksi khususnya bagian daun. Hal ini dikarenakan daun kemangi memiliki senyawa aktif seperti minyak atsiri, alkaloid, saponin, flavonoid, triterpenoid, steroid, tannin dan fenol yang dapat menghambat pertumbuhan bakteri. Tujuan penelitian ini dilakukan untuk mengetahui ada tidaknya aktivitas, mengetahui diameter zona hambat dan mengetahui klasifikasi kekuatan aktivitas daya hambat antibakteri ekstrak etanol daun kemangi. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi lubang sumuran dengan teknik pengambilan sampel adalah purposive sampling. Konsentrasi ekstrak yang digunakan yaitu konsentrasi 100%, 80%, 60%, 40%, 20%, sedangkan untuk kontrol positif digunakan klindamisin 30µg, dan kontrol negatif yang digunakan etanol 96%. Hasil diameter zona hambat yang terbentuk diukur dengan jangka sorong. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kemangi memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter rata-rata yang didapat dari setiap perlakuan yaitu 100% (10,08 mm), 80% (8,10 mm), 60% (6,49 mm), 40% (4,29 mm), 20% (2,26 mm), dan sebagai klasifikasi kekuatan aktivitas daya hambat antibakteri yaitu pada konsentrasi 100% kuat, 80%-60% sedang dan 40%-20% lemah. Kata Kunci : Daun kemangi, Ekstrak, Difusi, Staphylooccus aureus ABSTRACT Part of the basil plant (Ocimum sanctum L.) that widely used by people for treatment of infections is basil leaves. This is because basil leaves have active compounds such as essential oils, alkaloids, saponins, flavonoids, triterpenoids, steroids, tannins and phenols which can inhibit bacterial growth. This research aimed to find out the presence or absence of activity, to determine the diameter of the inhibitory zone and the classification of antibacterial mention against what the name of bacterial is activity of ethanol extract of basil leaves. The type of this research is experimental research with a well diffusion method with sampling technique is purposive sampling. The concentration of extracts used were concentrations of 100%, 80%, 60%, 40%, 20%, while as positive control is clindamycin 30µg, and the negative control used 96% ethanol. The resulting diameter of the inhibition zone is measured by the calipers. The results showed that the ethanol extract of basil leaves had an activity in inhibiting the growth of Staphylococcus aureus bacteria with an average diameter obtained from each treatment that was 100% (10,08mm); 80% (8,10mm); 60% (6,49mm); 40% (4,29mm); 20% (2,26mm), and as the antibacterial activity classification, that were strong in 100% of extract concentration, medium in 60-80% of extract concentration, and weak in 20-40% of extract concentration. Keywords : Basil leaf, Extract, Diffusion, Staphylooccus aureus

Download Full-text

FORMULASI DAN UJI EFEKTIVITAS KRIM ANTIBAKTERI EKSTRAK DAUN NANGKA (Artocarpus heterophyllus Lam.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽

10.35799/pha.8.2019.29257 ◽

2019 ◽

Vol 8 (1) ◽

pp. 225

Author(s):

Nurul S Majid ◽

Paulina V. Y. Yamlean ◽

Gayatri Citraningtyas

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Physical Stability ◽

Ethanol Extract ◽

Inhibition Zone ◽

Quality Test ◽

Antibacterial Compounds ◽

Artocarpus Heterophyllus ◽

Test Parameters

ABSTRACT Jackfruit leaves contain saponins, flavonoids and tannins. Which are know to have antibacterial compounds. The purpose of this study is to make cream of jackfruit leaf extract with a concentration of 5%, 10% and 15% and test the quality of preparations of jackfruit leaf extract cream and there is antibacterial effectiveness against Staphylococcus aureus bacteria. The method used is the ethanol extract of Jackfruit leaves by formulating it as an M/A type cream. The results showed the cream of jackfruit leaf extract fulfilled all the physical stability tests of the cream and in the antibacterial testing of cream of Jackfruit leaf extract could inhibit the effectiveness of Staphylococcus aureus bacteria. As concluded, the Jackfruit leaf extract Conclusions obtained by jackfruit leaf extract can be formulated as a cream with a concentration of 5%, 10% and 15%, and cream preparations meet the cream quality test parameters, for testing the effectiveness of antibacterial to the biggest inhibition zone Staphylococcus aureus is 10.5 mm at 15% concentration. Keywords: Jackfruit leaves, cream antibacterial, Staphylococcus aureus. ABSTRAK Daun Nangka memiliki kandungan saponin, flavonoid dan tannin yang berperan sebagai senyawa antibakteri. Tujuan penelitian ini yaitu membuat krim ekstrak daun Nangka dengan konsentrasi 5%, 10% dan 15%, menguji mutu dan efektivitas antibakteri krim ekstrak daun Nangka terhadap bakteri Staphylococcus aureus. Metode yang digunakan yaitu ekstrak etanol daun Nangka dengan memformulasikan sebagai krim dengan tipe M/A. Hasil penelitian menunjukkan krim ekstrak daun Nangka memenuhi semua uji kestabilan fisik krim dan dalam pengujian antibakteri krim ekstrak daun Nangka dapat menghambat efektivitas bakteri Staphylococcus aureus. Kesimpulan yang diapat ekstrak daun Nangka dapat diformulasikan sebagai krim dengan konsentrasi 5%, 10% dan 15%, dan sediaan krim memenuhi parameter uji kualitas krim, untuk pengujian efektivitas antibakteri terhadap Staphylococcus aureus zona hambat terbesar yaitu 10,5 mm pada konsentrasi 15%. Kata kunci: Daun Nangka, krim antibakteri, Staphylococcus aureus.

Download Full-text

Uji daya hambat ekstrak daun cengkih (Syzygium aromaticum (L.) ) terhadap bakteri Enterococcus faecalis

e-GIGI ◽

10.35790/eg.5.1.2017.15547 ◽

2017 ◽

Vol 5 (1) ◽

Author(s):

Eskha M. Lambiju ◽

Pemsi M. Wowor ◽

Michael A. Leman

Keyword(s):

Enterococcus Faecalis ◽

Leaf Extract ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Positive Control ◽

Syzygium Aromaticum ◽

Normal Flora ◽

Gram Positive Bacteria ◽

Modified Method ◽

Growth Of Bacteria

Abstract: Cloves (Syzygium aromaticum L.) grow widely in Indonesia. This plant has many benefits from its stem, leaves, and flowers. Clove leaves has several antibacterial compounds such as flavonoids, tannins, and triterpenoids, as well as eugenol as the major component of essential oil. Enterococcus faecalis is a facultative anaerobic Gram positive bacteria and normal flora in the mouth. These bacteria are often identified as the cause of the failure of root canal treatment. This study was aimed to determine the effectiveness of clove in inhibiting the growth of bacteria Enterococcus faecalis. This was an experimental study with a modified method of Kirby-Bauer using pits. Samples of clove leaves were obtained from Treman, North Minahasa, and then were extracted by using maceration method with 96% ethanol. Metronidazole was used as positive control. Enterococcus faecalis bacteria were taken from the direct isolation of patients’ necrotic teeth. The results showed that the average inhibition zone of clove leaf extract against Enterococuss faecalis was 8.0 mm meanwhile of metronidazole was 10.0 mm. Conclusion: Clove leaf extract had moderate inhibitory effect against the growth of Enterococcus faecalis.Keywords: clove leaves, Enterococcus faecalis, inhibition. Abstrak: Tanaman cengkih (Syzygium Aromaticum L.) banyak tumbuh di Indonesia. Tanaman ini memiliki banyak manfaat mulai dari batang, daun, dan bunga. Daun cengkih mengandung berbagai senyawa yang bersifat antibakteri seperti flavonoid, tannin, dan triterpenoid, serta senyawa eugenol yang merupakan komponen utama dalam minyak atrisi. Enterococcus faecalis ialah bakteri Gram positif fakultatif anaerob yang merupakan flora normal dalam mulut. Bakteri ini sering terisolasi sebagai penyebab kegagalan perawatan saluran akar. Penelitian ini bertujuan untuk mengetahui daya hambat daun cengkih (Syzygium Aromaticum L.) terhadap pertumbuhan bakteri Enterococcus faecalis. Jenis penelitian ialah eksperimental dengan metode modifikasi Kirby-Bauer menggunakan sumuran. Sampel daun cengkeh diperoleh dari daerah Treman Kabupaten Minahasa Utara yang kemudian diekstrasi dengan metode maserasi menggunakan etanol 96%. Sebagai kontrol positif diginakan metronidazole. Bakteri Enterococcus faecalis diambil dari isolasi langsung pada pasien dengan gigi nekrosis. Hasil penelitian ini mendapatkan rerata zona inhibisi ekstrak daun cengkih terhadap bakteri Enterococuss faecalis sebesar 8,0 mm sedangkan zona inhibisi metronidazole 13,0 mm. Simpulan: Ekstrak daun cengkih memiliki daya hambat yang tergolong sedang terhadap pertumbuhan bakteri Enterococcus faecalis.Kata kunci: daun cengkih, Enterococcus faecalis, daya hambat

Download Full-text

ANTIBACTERIAL ACTIVITY OF MIANA LEAF ESTRACT (Coleus atropurpereus) ON Steptococcus aureus

Media Kesehatan Politeknik Kesehatan Makassar ◽

10.32382/medkes.v15i1.1033 ◽

2020 ◽

Vol 15 (1) ◽

pp. 1

Author(s):

Anita Anita ◽

Mujahidah Basarang ◽

Rahmawati Rahmawati

Keyword(s):

Antibacterial Activity ◽

Leaf Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Laboratory Observation ◽

Extract Concentration ◽

Observation Method ◽

The One ◽

Growth Of Bacteria

ABSTRACTMiana leaf(Coleus atrropurpureus) is the one of biofarmacology plant which using for antibacterial activity.Miana leaf contains flavonoid, tanin, saponin which can inhibit growth of bacteria. The aims this research were study antibacterial activity of miana leaf extract on Streptococcus aureus.This research used laboratory observation method which used miana leaf extract concentration 250 mg/ml, 125 mg/ml, 62.5 mg/ml, 31,25 mg/ml, and 15,625 mg/ml. This research used tetracylin for positive control and aquadest for negative control which growth 0n 37o C used difusion agar method. The results showed antibactrila activity miana leaf extract with a concentration 250 mg/ml with inhibition zone 0.00 mm, 125 mg/ml with inhibition zone 0,00 mm, 62,5 mg/ml with inhibition zone 0,00 mm, 31,25 mg/ml with inhibition zone 0,00 mm, and 15,62 mg/ml with inhibition zone 0,00 mm. For positive control showed inhibition zone 27,6 mm and negative control with inhibition zone 0,00 mm. Miana leaf extract concentration 250 mg/ml, 125 mg/ml, 62,5 mg/ml, 31,25 mg/ml and 15,62 mg/ml were not effectively inhibit Streptococcus sp.

Download Full-text

Uji daya hambat ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans secara in vitro

e-GIGI ◽

10.35790/eg.5.1.2017.14701 ◽

2017 ◽

Vol 5 (1) ◽

Author(s):

Olivia C. Simatupang ◽

Jemmy Abidjulu ◽

Krista V. Siagian

Keyword(s):

Candida Albicans ◽

Leaf Extract ◽

In Vitro Study ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Morinda Citrifolia ◽

Predisposing Factor ◽

Group Design ◽

Post Test

Abstract: Candida albicans is a normal flora of the mouth, respiratory tract, gastrointestinal tract and the female genital but it becomes pathogen if there is a predisposing factor. Approximately 85-95% of oral candida infections are caused by C. albicans that is usually attached to the labial mucosa, buccal mucosa, the dorsal parts of the tongue and palate. Noni (Morinda citrifolia L.) is already known as a medical plant; one of it parts that has a medicinal effect is the leaf that contains anthraquinon as an antifungal. This study was aimed to determine the effect of noni leaf extract to the growth of C. albicans. This was a true experimental in vitro study with the post test only group design. The Kirby-Bauer method with filter paper was used to evaluate the sensitivity of C. albicans to mengkudu leaf extract. Leaves samples were extracted by using maceration process with ethanol 96%. C. albicans obtained from the pure stock of Laboratory of Microbiology Pharmacy Faculty of Math and Science, University of Sam Ratulangi. The results showed that the inhibition zone diameter of noni leaf extract on the growth of C. albicans was 16 mm (strong inhibition category). Conclusion: Noni leaf extract (Morinda citrifolia L.) had an inhibitory effect on Candida albicans.Keywords: Mengkudu leaf (Morinda citrifolia L.), Candida albicans, inhibition zone Abstrak: Candida albicans merupakan anggota flora normal rongga mulut, saluran pernafasan, saluran pencernaan, dan genital wanita namun dapat menjadi patogen jika terdapat faktor predisposisi. Sekitar 85-95% infeksi kandidiasis oral disebabkan oleh C albicans yang biasanya melekat pada mukosa labial, mukosa bukal, dorsum lidah dan palatum. Tanaman mengkudu (Morinda citrifolia L.) telah dikenal sebagai tumbuhan obat. Salah satu bagian tanaman mengkudu yang memiliki efek obat ialah daunnya karena adanya kandungan antrakuinon yang bersifat antijamur. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun mengkudu terhadap pertumbuhan C. albicans. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only group design. Pengujian menggunakan metode Kirby-Bauer berbahan kertas saring. Sampel daun diekstraksi dengan proses maserasi menggunakan etanol 96%. Jamur C. albicans diambil dari stok biakan jamur murni Laboratorium Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan diameter zona hambat ekstrak daun mengkudu terhadap pertumbuhan C. albicans 16 mm yang tergolong kriteria zona hambat kuat. Simpulan: Ekstrak daun mengkudu (Morinda citrifolia L.) mempunyai daya hambat terhadap jamur Candida albicans.Kata kunci: daun mengkudu, Candida albicans, zona hambat

Download Full-text