scholarly journals FORMULASI SEDIAAN GEL EKSTRAK ETANOL DAUN SENDUDUK (Melastoma malabathricum L.) TERHADAP UJI KESTABILAN FISIK DAN UJI AKTIVITAS ANTIBAKTERI PADA STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 15 (2) ◽  
pp. 88-93
Author(s):  
Dewi Marlina
Keyword(s):  
Staphylococcus Aureus ◽  
Gelling Agent ◽  
Melastoma Malabathricum ◽  
One Way Anova

Telah dilakukan penelitian tetang Formulasi Sediaan Gel Ekstrak Etanol Daun Senduduk (Melastoma malabathricum L.) Terhadap Uji Kestabilan Fisik dan Uji Aktivitas Antibakteri  Pada  Staphylococcus Aureus. Penelitian ini bersifat eksperimental dengan membuat 3 jenis formula gel yang mengandung ekstrak etanol daun senduduk (Melastoma malabathricum L.) 4% dengan memvariasikan HPMC sebagai gelling agent pada konsentrasi 3%, 3,5% dan 4%. Uji aktivitas antibakteri dilakukan dengan metode difusi agar terhadap bakteri Staphylococcus aureus. Sediaan disimpan selama 28 hari dan dievaluasi pH, viskositas, homogenitas, intensitas warna dan bau serta iritasi kulit  pada hari ke-0, ke-7, ke-14, ke-21 dan ke-28. Metode: Data uji kestabilan fisik yang diperoleh disajikan dalam bentuk tabel dan grafik lalu diinterprestasikan. Sedangkan untuk uji aktivitas antibakteri cara pengolahan dan analisis datanya yaitu menggunakan analisa statistika  One Way ANOVA yang dilakukan dengan cara mengukur diameter zona hambat. Hasil:  menunjukan bahwa semakin tinggi konsentrasi HPMC (gelling agent) maka semakin tinggi viskositas dan semakin rendah daya sebar dan pH, namun tidak berpengaruh terhadap homogenitas, warna, bau dan iritasi kulit. Hasil uji antibakteri menunjukkan bahwa pada formula ekstrak I zona hambat berkisar 11 – 16,5 mm, formula II zona hambat berkisar  10 – 16 mm dan pada formula III   zona hambat berkisar 10 – 14,5 mm.   Uji statistik oneway anova (p<0,05) untuk membandingkan zona hambatan pada sediaan pada hari ke-0, ke-7, ke-14, ke-21 dan ke-28, didapatkan signifikasi 0,412 (p>0.05). Kesimpulan:  Sediaan gel yang mengandung Ekstrak etanol daun senduduk pada Formula I mempunyai kestabilan fisik yang memenuhi persyaratan untuk dibuat sediaan gel.

scholarly journals Optical characterization and concentration-dependent antibacterial activity of silver nanoparticles (AgNPs) using aqueous Melastoma malabathricum extract against Staphylococcus aureus

2020 ◽  
Vol 11 (4) ◽  
pp. 5382-5387
Author(s):  
Irshad Ul Haq Bhat ◽  
Maisarah Binti Alias
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Functional Groups ◽  
Diffusion Method ◽  
Synthetic Approach ◽  
Potential Candidate ◽  
X Ray ◽  
Melastoma Malabathricum ◽  
Resonance Band

The approach towards green synthetic methods has been enormously encouraged to synthesise nanoparticles for various uses. In this study, the one-pot synthetic method was adapted to synthesise silver nanoparticles (AgNPs) using Melastoma malabathricum (M. malabathricum) aqueous extract. The formation of AgNPs was confirmed by observing the results obtained by optical characterisation methods. The plasma resonance band along with shoulder at 375 nm and 595 nm, respectively, in Uv-Visible spectra supported the conversion of silver (Ag) to AgNPs reduced by functional groups present in the plant extract. The size of AgNPs was 31 nm and cubic in shape as confirmed by X-ray diffractometry (XRD) using Scherer equation. X-Ray Fluorescence (XRF) results also confirmed the presence of silver. The FTIR characterisation confirmed the presence of reducing functional groups. The antibacterial activity of AgNPs against Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of AgNPs, and enhanced inhibition zone was observed. The AgNPs obtained can be further explored against different bacterial strains and can a potential candidate as an antibacterial agent using the green synthetic approach.

scholarly journals FORMULASI MASKER GEL PEEL-OFF PERASAN LIDAH BUAYA (Aloe vera L.) DENGAN GELLING AGENT POLIVINIL ALKOHOL

2020 ◽  
Vol 2 (1) ◽  
pp. 17-25
Author(s):  
Ivan Santoso ◽  
Tria Prayoga ◽  
Ika Agustina ◽  
Wiwit Setya Rahayu
Keyword(s):  
Polyvinyl Alcohol ◽  
Aloe Vera ◽  
The Other ◽  
Local People ◽  
Gelling Agent ◽  
Drying Time ◽  
Other Hand ◽  
One Way Anova ◽  
Made In

Aloe vera (Aloe Vera L.) is a plant which commonly used by local people as moisturizer. The usage of aloe vera can be applied in form of peeled off gel mask with polyvinyl alcohol as gelling agent. The goal of this research is to determine the influence of increase of polyvinyl alcohol as gelling agent to the formulation of peeled off mask from aloe vera juice. The formulation was made in a few concentration, 10%, 12%, and 14% by adding 0,5% of aloe vera juice. After that, the formulation evaluated for 4 weeks about the organoleptics, homogenity, pH, drying time and viscosity. The date of pH test and drying time analyzed by using one way ANOVA statistically and then followed by Tukey HSD test and the viscosity analyzed by using Kruskall Wallis statistically which result in the significantcy less than 0,05 that mean there are difference in drying time and viscosity, on the other hand there is no difference in pH.

scholarly journals Formulasi dan Evaluasi Fisik Sediaan Emulgel Tabir Surya Ekstrak Kulit Batang Kayu Manis (Cinnamomum burmannii)

2019 ◽  
Vol 5 (1) ◽  
pp. 90-99
Author(s):  
Risqika Yuliatantri Paramawidhita ◽  
Uswatun Chasanah ◽  
Dian Ermawati
Keyword(s):  
Chemical Constituents ◽  
Physical Characteristics ◽  
Gelling Agent ◽  
Bark Extract ◽  
Cinnamon Bark ◽  
Cinnamomum Burmannii ◽  
Dispersive Power ◽  
One Way Anova ◽  
Optimal Formula ◽  
Brown Color

One of the natural ingredients can be used for sunscreen is Cinnamomum burmannii. Sinamaldehid chemical constituents in cinnamon bark extract that has potential as an antioxidant and can be used as a sunscreen content. This study was to determine the levels of cinnamon bark extract ( 1 %, 2 % and 3 % ) which can give physical characteristics, acceptability, which is optimal for preparations with HPMC as a gelling agent, Evaluations include pH, the power spread, the viscosity and acceptability. The results of organoleptic for formula I (1%), II (2%) and III (3%) have a soft texture grainy, the distinctive smell of cinnamon, and all formula has a pale brown color. From the analysis of One-Way ANOVA found significant differences for any dispersive power. But did not have significant differences in pH and viscosity. For acceptability evaluation formula III is the optimal formula to applied softness, easiest formula to flattened and easy to wash.

scholarly journals UJI AKTIVITAS ANTIBAKTERI FRAKSI DAUN BIDURI (Calotropis gigantea) TERHADAP Staphylococcus aureus

2021 ◽  
Vol 4 (1) ◽  
pp. 40-45
Author(s):  
Nurul Hidayah ◽  
Choirul Huda ◽  
Dara Pranidya Tilarso
Keyword(s):  
Staphylococcus Aureus ◽  
Calotropis Gigantea ◽  
One Way Anova

Staphylococcus aureus adalah bakteri Gram positif yang berbentuk menggerombol. Bakteri Staphylococcus aureus merupakan penyebab utama infeksi nosokomial, keracunan makanan, dan sindrom syok toksik. Infeksi Staphylococcus aureus dapat terjadi ketika sistem imun melemah. Salah satu penanganan infeksi adalah menggunakan antibiotik, namun penggunaan antibiotik yang tidak tepat akan menimbulkan resistensi, sehingga terapi menggunakan obat tradisional menjadi pilihan alternatif. Obat tradisional yang berasal dari tanaman memiliki efek samping yang jauh lebih rendah tingkat bahayanya dibandingkan obat-obatan kimia. Salah satu tumbuhan yang dapat dimanfaatkan sebagai bahan obat adalah tanaman biduri (Calotropis gigantea L.). Tujuan penelitian ini adalah mengetahui aktivitas fraksi Aquadestilata, etil asetat dan n-heksan dari ekstrak daun biduri sebagai antibakteri terhadap bakteri Staphylococcus aureus. Daun biduri diekstraksi menggunakan metode maserasi dengan menggunakan pelarut 70% dan difraksinasi menggunakan pelarut Aquadestilata, etil asetat dan n-heksan. Uji aktivitas antibakteri fraksi daun biduri menggunakan metode difusi cakram dengan seri konsentrasi 10%, 20% dan 30%. Analisis statistik yang digunakan yaitu one way ANOVA yang bertujuan untuk mengetahui  berpengaruh atau tidaknya variasi konsentrasi fraksi daun biduri terhadap pertumbuhan bakteri Staphylococcus aureus. Hasil penelitian yang telah dilakukan menunjukkan bahwa fraksi Aquadestilata dan etil asetat mempunyai aktivitas antibakteri. Fraksi Aquadestilata merupakan fraksi teraktif dalam menghambat aktivitas bakteri Staphylococcus aureus karena mengandung senyawa flavonoid, tanin dan saponin. Konsentrasi optimum fraksi daun biduri dalam menghambat pertumbuhan bakteri Staphylococcus aureus adalah 10% dengan rata-rata zona hambat sebesar 6,33 mm.  

POTENSI EKSTRAK DAUN TANAMAN KARAMUNTING (Melastoma malabathricum L.) DI DAERAH KALIMANTAN SEBAGAI ANTIBAKTERI Staphylococcus aureus

2018 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Rakhmadhan Niah ◽  
Riki Nirwan Baharsyah
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
In Vitro Studies ◽  
Plant Leaf ◽  
Extract Concentration ◽  
Melastoma Malabathricum ◽  
Agar Method ◽  
Microbiology Research ◽  
Growth Of Bacteria

The year 2008 recorded the death toll reached 16,769 soul due to infection. Infection is one of the diseases caused by bacteria or parasites. Staphylococcus aureus is one of the bacteria causes infection of the skin that is often the case in Indonesia. Staphylococcus aureus bacterial infection can be treated naturally, one of the plants that can be utilized is plant karamunting. Plant leaf karamunting (Melastoma malabathricum l.) contain flavonoids and alkaloids that have activity as antibacterial. The purpose of this research is to know the power of drag karamunting folium of extract against the growth of Staphylococcus aureus. In vitro studies were conducted with diffusion agar method. The extraction of leaf maceration method done with karamunting solvent ethanol 96%. Concentration of karamunting folium of extract used are: 25%, 50%, 75%, and 100%. Microbiology research results indicate that karamunting folium of extract have drag against the growth of bacteria Staphylococcus aureus with extract concentration 50% yield of 5.34 mm; the concentration of extract 75% of 9.40 mm; the concentration of extract 100% of 12.43 mm.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI ISOLAT CENDAWAN ENDOFIT ASAL TUMBUHAN SENDUDUK (Melastoma malabathricum L.) TERHADAP Escherichia coli DAN Staphylococcus aureus

2018 ◽  
Vol 8 (2) ◽  
pp. 7-12
Author(s):  
Israwati Harahap ◽  
Vivin Paddillah Rahmi ◽  
Nofripa Herlina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Zone Of Inhibition ◽  
Melastoma Malabathricum ◽  
Symbiotic Microorganism ◽  
And Staphylococcus Aureus ◽  
Test Result

Endophytic fungi is a symbiotic microorganism which live inside plant tissues and not harm to their host. Several genera from endophytic fungi known to produce secondary metabolite compounds like antibiotics, anticancer, antifungal, antiviral and antimalarial. This study aimed to investigate antibacterial activity of endophytic fungi from senduduk (Melastoma malabathricum L.) against Escherichia coli and Staphylococcus aureus. Based on the test result, 11 isolates endophytic fungi from senduduk were known to have antibacterial activities. Isolate code 23 have ability to inhibit the growth of E.coli with diameters zone of inhibition is 22 mm and eight isolate endophytic fungi have ability to inhibit the growth of S.aureus (showed by inhibition zone).

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals Pengaruh Ozonated Water sebagai Antiseptik dalam Menghambat Pertumbuhan Staphilococcus Aureus (in vitro)

2012 ◽  
Vol 19 (1) ◽  
pp. 25
Author(s):  
Yulita Kristanti ◽  
D. Dessy
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Negative Control ◽  
Strong Negative Correlation ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
One Way Anova ◽  
Ozonated Water

Latar Belakang. Penelitian seputar ozonazed water akhir-akhir ini cukup banyak diminati karena bahan ini mempunyai potensi antibakteria yang menjanjikan. Tujuan Penelitian ini adalah untuk mengetahui pengaruh ozonated water pada pertumbuhan bakteri Staphylococcus Aureus. Metode Penelitian. Penelitian ini dilakukan dengan cara mencampurkan 1 ml larutan bakteri 106 CFU/ml dengan 10 ml ozonated water konsentrasi 4 ppm selama 10 detik (kelompok I), 20 detik (kelompok II), 30 detik  (kelompok III), dan 40 detik (kelompok IV), akuades (kontrol negatif) kemudian semua diencerkan dengan akuabides 10ml. selanjutnya dari masing-masing kelompok diambil 0,01 ml untuk ditanam pada MHA, inkubasi 24 jam pada suhu 370 C dalam anaerobic jar kemudian dilakikan perhitungan koloni. Data dianalisis dengan Avana satu jalur dilanjutkan dengan uji LSD. Hasil. Avana satu jalur memperlihatkan adanya perbedaan yang bermakna antar kelompok perlakuan. Uji LSD menunjukkan perbedaan rerarta yang bermakna (p<0,05) antar kelompok I, II, III, IV dengan kelompok V (akuedes) terdapat perbedaan rerata yang bermakna antara kelompok I dan kelompok VI (iod). Tidak terdapat perbedaan bermakna antara kelompok II, III, IV terdapat kelompok VI (oid). Uji korelasi Produk Momen Pearson menunjukkan adanya korelasi negatif yang sangat kuat. Kesimpulan. Ozonated Water 4 ppm memiliki daya antibakreri dalam menghambat pertumbuhan Staphylococcus Aureus. Semakin lama waktu kontak, daya anti bakterinya semakain besar. Background. Some previous research show that the antibacterial effect of ozonated water is very promi-sing. The aim of this study was to know the antibacterial effect of ozonated water on staphylococcus aureus. Method. One ml of 106 CFU/ ml S. aureus suspension was mixed with 10 ml ozonated water for 10 second (group I), 20 second (group II), 30 second (group III), 40 second (group IV)As negative control S. aureus was mixed with aquadest (group V), and as positive control S. aureus was mixed with iod (groupVI). Furthermore, 0,01 ml from each group was cultivated on MHA, incubated for 24 hours-37C followed by colony caunting. Data was analyzed using one way anova followed by LSD. Result. One way anova show significant difference among the group and LSD test show significant mean different between group I, II, III, IV, and V. Significant difference can also be seen between group I and V. No significant difference between group II, III, IV and VI. Product momen test show strong negative correlation. Conclusion. Ozonated water 4 ppm has antibacterial effect on S. aureus. The longer the contact time, the stronger the antibacterial effect.

scholarly journals Formulation of Toothpaste Toothpaste Gel Essential Oil of Lime Leaves (Citrus Aurantifolia) With Variations Concentration of Carbomer 940 As Gelling Agent Base

2021 ◽  
Vol 4 (2) ◽  
pp. 98-103
Author(s):  
Nurista Dida Ayuningtyas ◽  
Agustina Putri Pitarisa Sudarsono ◽  
Anastasiya Sisca Yuswanti
Keyword(s):  
Leaf Extract ◽  
Statistical Tests ◽  
Gelling Agent ◽  
Spreading Area ◽  
Significant Difference ◽  
Independent Variable ◽  
Spss Software ◽  
Ph Range ◽  
Carbomer 940 ◽  
One Way Anova

Background : Lime leaves contain flavonoids, tannins, and saponins. This content is known to have activity as an antibacterial and protective of gums and teeth. Based on these activities, lime leaves can be developed as an active ingredient in gel toothpaste preparations. In the manufacture of gel toothpaste, the component that plays an important role is the gelling agent. Purpose: In this study, a variation of the gelling agent Carbomer 940 will be used to make a gel toothpaste with lime leaf extract. Methods: The independent variable used is Carbomer 940 with a variation of 3 formulas, namely 0.6%; 1.2%; and 1.8%. The evaluations carried out on gel toothpaste were organoleptic, homogeneity, pH, adhesion, spreadability and pH. Data analysis was carried out with SPSS software to see the differences between each formula. Results: The results showed that the formula 1-3 was homogeneous with a clear, homogeneous color, and a characteristic smell of menthol with a pH range of 5.85-7.49; spreading area 20.60-29.91 cm2, adhesion 6.01-35.1 seconds. The results of statistical tests with one-way ANOVA obtained a significant difference between each formula.  

scholarly journals FORMULASI SEDIAAN GEL SCRUB WAJAH SERBUK BIJI KOPI ARABIKA (Coffee arabica) DENGAN KONSENTRASI KARBOPOL 940 SEBAGAI GELLING AGENT

2021 ◽  
Vol 4 (2) ◽  
pp. 68-73
Author(s):  
Natasha Faruqi Andrian Putri ◽  
Desy Nawangsari ◽  
Sunarti Sunarti
Keyword(s):  
Gelling Agent ◽  
Coffee Arabica ◽  
One Way Anova

INTISARI Kopi merupakan tanaman yang kaya akan manfaat. Biji kopi arabika mengandung antioksidan yang dapat memberikan manfaat salah satunya mencegah kerusakan sel akibat paparan radikal bebas. Kopi arabika di formulasikan dalam bentuk gel scrub dengan gelling agent karbopol 940. Penelitian ini bertujuan untuk mengetahui berapa konsentrasi gelling agent yang terbaik terhadap sifat fisika dan stabilitas sediaan gel srub biji kopi arabika (Coffee arabica) dan pada saat digunakan menyebabkan iritasi atau tidak. Metode penelitian eksperimental murni untuk mengetahui pengaruh perlakuan tertentu dengan membandingkan dengan kelompok kontrol yang tidak diberikan perlakuan. Evaluasi sediaan gel scrub meliputi evaluasi mutu fisik sediaan dan uji iritasi terhadap 3 kelinci. Hasil evaluasi sediaan menunjukkan bahwa semua sediaan gel scrub berbentuk gel dengan butiran berwarna hitam, berbau khas, homogen, stabil selama penyimpanan 6 siklus, nilai pH (4,5-7,8), daya lekat (2-300 detik), daya sebar (5-7 cm) dan viskositas (2000-4000 cp), uji iritasi pada kelinci menyebabkan reaksi kulit sedikit iritasi. Hasil uji statistik dengan uji one way anova menunjukkan terdapat perbedaan yang signifikan antar formula dengan nilai signifikasi (p< 0,05). Namun sediaan masih bisa dikatakan stabil dikarenakan masih berada pada rentang persyaratan. Kesimpulan gel scrub yang dihasilkan memiliki karakteristik fisik yang baik dan menyebabkan reaksi kulit sedikit iritasi. Serbuk biji kopi arabika dapat diformulasikan sebagai sediaan gel scrub memiliki karakteristik fisik yang baik pada formula IV dengan konsentrasi karbopol 940 2 %. dan stabil pada penyimpanan 6 siklus.

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