scholarly journals Effects of aqueous seed extract of Moringa oleifera (L) on haematology, gonadotropins and testosterone profiles of Yankasa rams

2020 ◽  
pp. 27-33
Keyword(s):  
Luteinizing Hormone ◽  
Dose Rate ◽  
Blood Cells ◽  
Moringa Oleifera ◽  
White Blood Cells ◽  
Basal Diet ◽  
Seed Extract ◽  
Luteinizing Hormone Level ◽  
Group A ◽  
And Control

Introduction: Moringa oleifera is a multipurpose plant, which belongs to the family Moringaceae, and is recognised globally. Moringa oleifera aqueous seed extract was used to determine its effects on the haematology and some reproductive hormone profiles in Yankasa rams. Methods: Twenty-Five Yankasa rams weighing 18-20 kg, were purchased and kept at the experimental pen of the Department of Veterinary Parasitology and Entomology, Ahmadu Bello University, Zaria for this study. The rams were screened and acclimatized for 2 weeks prior to the commencement of the research. Thereafter, the rams were randomly divided into five groups A, B, C, D and E with five rams each as treatment and control groups respectively. The rams were fed basal diet of Digitaria smuttssi. Water was provided ad libitum. Group A, B, C, D and E were given oral dose of M. oleifera aqueous seed extract and water daily at a dose rate of 1000, 2000, 3000, 4000 mg/kg and 10 ml/kg respectively. Blood samples were collected via jugular vein once every week to evaluate packed cell volume (PVC %), total red blood cells (TRBC× 1012/L) and Total white blood cells (TWBC × 1012/L). To obtain sera blood was collected 3 times at 30 minutes interval between 7:00 and 8:00 A.M, the sera were evaluated for follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone concentrations in Yankasa rams. Results: Haematology results revealed significant (p < 0.05) increase in PCV in group C (37.5 ± 0.7 %). There was significant (p < 0.05) increase in the testosterone and luteinizing hormone level at 7:00 A.M in group A (5.42 ± 0.1 ng/ml). Also, there was significant (p < 0.05) increase in the levels of FSH, LH and testosterone. Significance: In conclusion, treatment of Yankasa rams with M. oleifera aqueous seed extract at the dose rate of 1000-3000 mg/kg increased testosterone, PCV, LH and FSH profiles. Therefore, M. oleifera aqueous seed extract may be recommended as a fertility enhancing agent in Yankasa rams.

Significance of graded doses of aqueous seed extract of Moringa oleifera (L) on live body weight, gonadal, extragonadal dimensions and sperm reserves of Yankasa rams

2021 ◽  
pp. 33-40
Keyword(s):  
Body Weight ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Live Body Weight ◽  
And Control ◽  
Standard Techniques ◽  
Different Doses ◽  
Apparently Healthy

The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.

scholarly journals Effects of Fruits of Lapsi (Nepali Hog Plum) Choerospondias axillaris (roxb.) b.l.Burtt & a.w.Hill on Some Blood Parameters of Major Carp rohu Labeo rohita(H) During Intensive Aquaculture

2020 ◽  
Vol 19 (1) ◽  
pp. 186-195
Author(s):  
Shubha Ratna Shakya ◽  
Shyam Narayan Labh
Keyword(s):  
Blood Cells ◽  
Labeo Rohita ◽  
Control Diet ◽  
White Blood Cells ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Significant Difference ◽  
Fish Feeds ◽  
Intensive Aquaculture ◽  
And Control

 The complete blood cell count (CBC) is an important and powerful diagnostic tool to monitor the health status of fish in response to changes related to nutrition, water quality, and disease in response to therapy.Thus, the present study was about to know the effect of lapsi fruit pulp (Choerospondias axillaris) on some blood parameters in the fingerlings of rohu Labeo rohita cultured in Corona of Agriculture Hatchery farm, Chitwan, Nepal. Altogether eighteen hapas made of heavy-duty nylon net (1.5m3) were kept and placed inside the pond, distributed linearly, and then 270 fingerlings (@15 fishes/ hapa) were kept distributed randomly. Six practical diets like T1 (0.0 g kg-1), T2 (0.1 g kg-1), T3 (0.2 g kg-1), T4 (0.4 g kg-1), T5 (0.8 g kg-1) and T6 (1.6 g kg-1) were prepared. Feed containing 40% protein was supplemented with the ethanol extract of lapsi fruits. At the end of 90 days of the feeding trial, a significant difference (P< 0.05) in blood parameters were observed between the treated and control diet-fed groups. Hemoglobin (Hb), white blood cells (WBCs), red blood cells (RBCs), packed cell volume (PCV), and other erythrocyte indices were recorded higher in the treated groups. RBC, WBC, Hct, and Hb were found significantly higher in the T4 (0.4 %) diet-fed group. The study showed a minimum of 0.4 % (0.4g kg-1) lapsi fruit extract needed in fish feeds to increase blood parameters to enhance growth and immunity.

Non-specific protection of the body of la-boratory rats under the influence of experimental hypoxia

2020 ◽  
Vol 3 ◽  
pp. 116-123
Author(s):  
F.I. Alistratova ◽  
Keyword(s):  
Blood Cells ◽  
Blood Test ◽  
White Blood Cells ◽  
The Body ◽  
Urgent Problem ◽  
Adaptive Capabilities ◽  
First Case ◽  
Group A ◽  
Adaptive Reactions

To date, the search for the most informa-tive and simple methods for diagnose of adaptive capabilities is an urgent problem of veterinary medicine and adaptive physiolo-gy. The direction of the animal body's re-sponse to the hypoxic factor has not been sufficiently studied. Objective: the analyze of the dynamics of changes of the main indi-cators in the leukogram under the influence of hypoxia, as well as the study of the role of leukocyte, indices the analysis of the level of resistance in rats. The animals were divided into two groups: in the first case, the animals were injected with cytoflavin at a dose of 135 mg/kg before the hypoxia ses-sion, and in the second case, saline was ad-ministered at an equivalent dose. The blood test was performed on a hematological auto-matic analyzer, and the clinical parameters of peripheral blood were determined. Mathe-matically, the leukogram was used to deter-mine: the white blood cell shift index (ISLC), the lymphocyte-monocyte ratio in-dex (ISLM), and the lymphocyte-granulocyte index (ILG). The results of the study showed that the course of hypoxia contributes to a number of changes in the blood composition, for 21 days. There was an increase in the number of white blood cells in both groups, a change in the parame-ters of the leukocyte formula-a decrease in the content of lymphocytes, with a simulta-neous increase in the proportion of mono-cytes. The use of interval hypoxia caused a decrease in the content of red blood cells in animals of the first group, a decrease in the concentration of hemoglobin and hematocrit in both groups studied. The number of plate-lets increased, which also indicates the start of adaptive reactions in the body of rats. The use of leukocyte indices is the assess to the level of resistance of the body and showed their high significance and informa-tive value in modeling experimental hypoxia.

scholarly journals Silage quality, growth performance and haematology of West African dwarf goats fed Moringa oleifera leaves ensiled with cassava peels

2021 ◽  
Vol 48 (2) ◽  
pp. 183-190
Author(s):  
S. M. Odeyinka ◽  
T. O. Abegunde ◽  
M. O. Ofoegbu ◽  
O. J. Apanisile
Keyword(s):  
Chemical Composition ◽  
Blood Cells ◽  
Moringa Oleifera ◽  
White Blood Cells ◽  
Dry Season ◽  
West African ◽  
Daily Weight Gain ◽  
West African Dwarf ◽  
Moringa Oleifera Leaves ◽  
Cassava Peels

Forage scarcity in the dry season is challenging for ruminant production in the tropics. The utilization of browse species, particularly as silage can bridge this gap. In this study, the potentials of Moringa oleifera leaves ensiled with varying levels of cassava peels as dry season feed for West African dwarf (WAD) goats were assessed. Different mixtures of Moringa oleifera leaves and cassava peels were ensiled and designated as follows: T1= 100% Moringa, T2= 60% Moringa + 40% Cassava peels, T3= 40% Moringa + 60% Cassava peels, T4= 50% Moringa + 50% Cassava peels, T5= 100% Cassava peels. Chemical composition and silage characteristics were investigated. Twenty five WAD goats with average body weights of 6.02±0.71 kg were used in the growth study that lasted 12 weeks. Blood samples were collected from the goats through the jugular vein for haematological status at the beginning and end of the experiment. Chemical composition of the silage diets showed that it contained 27.79 – 36.26 % dry matter (DM), 7.45 – 22.25 % crude protein (CP), 8.92 – 16.45 % crude fibre (CF), 2.55 – 7.01 % ether extract (EE), 6.10 – 10.08 % ash content, 42.40 - 68.14 % Nitrogen Free Extract (NFE). Appearance, odour and texture of the silage had acceptable physical attributes with pH values ranging from 3.53 – 5.80. Average daily feed intake (ADFI) and Feed conversion ratio (FCR) were not significantly (P<0.05) affected by diets while Average daily weight gain (ADWG) was significantly improved in T3 (40% M. oleifera + 60% cassava peels) than in others. White blood cells (WBC) ranged from 5.88 x109/L in T2 to 13.77 x109/L in T4. Red blood Cells (RBC) and Packed Cell Volume (PCV %) were not significantly (P<0.05) affected by diets. It was concluded that Moringa oleifera leaves and cassava peels silage with 40% Moringa oleifera as additive elicited the best performance indices in WAD goats fed diets consisting of mixtures of Moringa oleifera leaves and cassava peels ensiled together in graded levels or alone and did not affect animals negatively in terms of their haematological components.     Moringa, T2= 60% Moringa + 40% Pelures de manioc, T3= 40% Moringa + 60% Pelures de manioc, T4= 50% Moringa + 50% Pelures de manioc, T5= 100% Pelures de manioc. Des caractéristiques chimiques de composition et d'ensilage ont été étudiées. Vingt-cinq chèvres 'WAD' avec un poids corporel moyen de 6,02±0,71 kg ont été utilisées dans l'étude de croissance qui a duré 12 semaines. Des échantillons de sang ont été prélevés sur les chèvres par la veine jugulaire pour le statut hématologique au début et à la fin de l'expérience. La composition chimique des régimes d'ensilage a montré qu'elle contenait 27.79 – 36,26 % de matière sèche (DM), 7.45 à 22.25 % de protéines brutes (CP), 8.45 à 22.25 % de protéines brutes (le 'CP'), 8.45 92 – 16.45 % de fibres brutes (le 'CF'), 2.55 à 7.01 % d'extrait d'éther (le 'EE'), 6.10 à 10.08 % de cendres, 42.40 - 68.14 % Extrait sans azote (le 'NFE'). L'apparence, l'odeur et la texture de l'ensilage avaient des attributs physiques acceptables avec des valeurs de pH allant de 3.53 à 5.80. L'apport quotidien moyen en aliments (le 'ADFI') et le ratio de conversion des aliments pour animaux (le 'FCR') n'ont pas été significativement (P<0.05) affectés par les régimes alimentaires, tandis que le gain quotidien moyen de poids (le 'ADWG') a été sensiblement amélioré en T3 (40 % M. oleifera + 60 % de pelures de manioc) que dans d'autres. Les globules blancs (le 'WBC') allaient de 5,88 x109/L en T2 à 13.77 x109/L en T4. Les globules rouges (le 'RBC') et volume de cellules emballées (le 'PCV' %) n'ont pas été significativement (P<0.05) affectés par les régimes. On a conclu que les feuilles de Moringaoleifera et l'ensilage des pelures de manioc avec 40 % de Moringaoleifera comme additif ont obtenu les meilleurs indices de performance chez les chèvres WAD nourries à des régimes composés de mélanges de feuilles de Moringaoleifera et de pelures de manioc ensilées ensemble ou seuls et n'affectaient pas négativement les animaux en termes de composantes hématologiques.

scholarly journals The utility of basic blood counts, WBC histogram and C-reactive protein in detecting malaria

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jun Nishimura ◽  
Parag Dharap ◽  
Sebastien Raimbault
Keyword(s):  
Blood Cells ◽  
Malaria Infection ◽  
Monsoon Season ◽  
Characteristic Curve ◽  
White Blood Cells ◽  
C Reactive Protein ◽  
Control Groups ◽  
Reactive Protein ◽  
Hematology Analyzer ◽  
And Control

Abstract Background Hematology analyzers display abnormal parameters during malaria infection providing insightful information for suspecting and assessing malaria infection. The goal of this study is to demonstrate the potential of a three-part differential hematology analyzer to assess malaria, provide information about the parasitemia, and discuss the importance of combining C-reactive protein (CRP) with hematology parameters to obtain further information about the malaria infection. Methods The present study shows the results of a case–control study during the monsoon season of years 2018 and 2019 in Mumbai, India. The study considers 1008 non-malaria febrile cases, 209 P. vivax and 31 P. falciparum positive malaria samples, five cases of mixed P. vivax and P. falciparum infection, and three co-infection cases of P. vivax and dengue. Raw data from the three-part analyzer LC-667G CRP (HORIBA) and the corresponding microscopic findings (golden standard for diagnosis of malaria) were obtained for each sample. Results The medians of platelet counts (PLT) were 102.5, 109.0, and 223.0 × 103/µL, while CRP medians were 67.4, 81.4 and 10.4 mg/L in P. vivax, P. falciparum and control groups respectively (p < 0.001 in Mann–Whitney U tests between malaria and control groups). Compared with negative samples, platelets counting less than 161.5 × 103/µL were observed on malaria patients (OR 19.12, 95% CI 11.89–30.75). Especially in P. vivax cases, an abnormal peak was frequently observed in the white blood cells (WBC) histogram around the 37fL channel. The events counted around that channel showed a linear correlation with the counting of red blood cells infected predominantly with larger parasitic forms. Parameters like CRP (rs = 0.325, p < 0.001), WBC (rs = 0.285, p < 0.001) and PLT (rs = − 0.303, p < 0.001) were correlated with the parasitemia of P. vivax samples. Between the malaria and dengue groups, the highest area under the receiver operating characteristic curve was observed on CRP (0.867, CRP ≥ 26.85 mg/L). Conclusions A three-part differential hematology analyzer has the potential to not only trigger malaria diagnosis confirmation but also assess the severity of the infection when CRP is considered.

scholarly journals Oxidative Effects of Potassium Dichromate on Biochemical, Hematological Characteristics, and Hormonal Levels in Rabbit Doe (Oryctolagus cuniculus)

2019 ◽  
Vol 6 (1) ◽  
pp. 30 ◽  
Author(s):  
Chongsi Mary Momo ◽  
Ngoula Ferdinand ◽  
Ngouateu Omer Bebe ◽  
Makona Alexane Marquise ◽  
Kenfack Augustave ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Luteinizing Hormone ◽  
Blood Cells ◽  
Potassium Dichromate ◽  
Follicle Stimulating Hormone ◽  
White Blood Cells ◽  
Reduction Process ◽  
Hepatic Tissue ◽  
Tissue Protein ◽  
Stimulating Hormone

The present study was conducted to evaluate the toxicity induced by the increasing doses of potassium dichromate in rabbit doe. Twenty-eight adult does of 6 months of age were divided into four groups (A, B, C, and D; n = 7), with comparable average body weight (bw). Group A rabbits received only distilled water daily and served as a control, while groups B, C, and D received, respectively, 10 mg/kg bw, 20 mg/ kg bw, and 40 mg/kg bw of potassium dichromate via gavage for 28 days, after which animals were anesthetized with ether vapor and sacrificed. Blood samples were obtained via cardiac puncture and collected without anticoagulant for biochemical dosages and with anticoagulant (EDTA) for complete blood count. Follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were dosed in serum and in homogenates of ovary with the help of AccuDiagTM ELISA kits from OMEGA DIAGNOSTICS LTD (Scotland, England) while respecting the immuno-enzymatic method. Activities of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and concentration of malondialdehyde (MDA) in liver, kidney, ovary and uterus were measured. Hematology revealed a significant (p < 0.05) decrease in mean values of hemoglobin and platelets while white blood cells and lymphocytes showed a significant (p < 0.05) increase in exposed groups. No significant (p > 0.05) difference was registered in monocytes, red blood cells, hematocrits, and plaquetocrits values with respect to the control. No matter the organ considered, no significant (p > 0.05) change was recorded in weight and volume. Nephrotoxicity analysis registered a significant (p < 0.05) increase in urea and creatinine, unlike renal tissue protein, which decreased significantly (p < 0.05). However, hepatotoxicity registered no significant (p > 0.05) variation in aspartate aminotransferase but total protein, alanine aminotransferase, and total cholesterol increased significantly (p < 0.05), while hepatic tissue protein revealed a significant (p < 0.05) decrease. Analysis on reproductive parameters showed a significant (p < 0.05) decrease in ovarian and uterine tissue proteins, as well as in follicle stimulating hormone, luteinizing hormone, and estradiol. Oxidative stress markers recorded no significant (p > 0.05) difference in glutathione reductase except in ovary where a significant (p < 0.05) decrease was seen when compared with the control, while catalase revealed a significant (p < 0.05) decrease, except in liver where there was no significant (p > 0.05) change. Superoxide dismutase and malondialdehyde recorded a significant (p < 0.05) decrease and increase respectively, with respect to the control. Results obtained from this study showed that the reduction process of chromium in tissues may cause the generation of reactive oxygen species, which are involved in hematoxic, nephrotoxic, hepatotoxic, and reproductive toxicity effects.

scholarly journals Reduction of Inflammatory C3 and C4 Complement Proteins in Severe COVID-19 Patients

2020 ◽  
Author(s):  
Ali Ghazavi ◽  
Ghasem Mosayebi ◽  
Nafiesh Keshavarzian ◽  
Somayeh Rabiemajd ◽  
Ali Ganji
Keyword(s):  
Blood Cells ◽  
Viral Infections ◽  
White Blood Cells ◽  
Control Group ◽  
Serum Samples ◽  
Complement Proteins ◽  
And Control ◽  
The Complement System ◽  
Shed Light

Abstract Background: The complement system, consisting of more than 20 soluble proteins, has a key role in innate immunity and inflammation that eliminates pathogens and viral infections. Therefore, we investigated the titer of C3, C4, and total IgG in the serum of the non-severe and severe COVID-19 patients. Methods: For this purpose, peripheral blood samples were collected from 30 non-sever, 30 severe COVID-19 patients, and 30 healthy individuals with similar age and sex as the control group. The amount of total IgG, C3, and C4 were analyzed in the serum samples. Also, white blood cells, platelets (PLTs), and lymphocytes were counted by the auto-analyzer. Results: White blood cells had no difference between patients and control groups. The results showed a significant decrease in lymphocyte and PLTs in COVID-19 patients compare to control. Complement proteins including C3 and C4 were increased in non-severe COVID-19 patients than the other groups. Total IgG showed a notable decrease in severe patients. In conclusion, the level of C3 and C4 complement proteins were increased in non-severe-COVID-19 patients; however, in the severe COVID-19 patients their concentrations were decreased. Conclusion: However, inflammatory C3 and C4 complement factors increase in non-severe COVID-19, it decreased in the severe patients that may be because of more consumption by the formation of the immune complex. These results can shed light on the inflammatory role of C3 and C4 proteins in various phases of the disease and could provide a basis for further exploration of the pathophysiological significance and can suggest them for specific interventions.

scholarly journals Evaluation of Gamavit effectiveness for correction of renal anemia in cats with chronic kidney disease: a controlled randomized study

2020 ◽  
Vol 2020 (5) ◽  
pp. 8-20
Author(s):  
Roman Leonard ◽  
Tat'yana Zaripova ◽  
Aleksandr Balahonov ◽  
Natal'ya Vdovina ◽  
Aleksandr Narovlyanskiy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Anemia ◽  
Control Group ◽  
Control Groups ◽  
Ckd Stage ◽  
Group A ◽  
And Control ◽  
Experimental Group ◽  
Stimulation Of

Renal anemia is one of the most common complications of chronic kidney disease (CKD) in cats, which significantly reduces their life quality. Most often, the mechanism of renal anemia development is associated with insufficiency of endogenous erythropoietin (EPO), or with a reduction in the life of red blood cells caused by uremic intoxication. Since anemia increases the severity of renal hypertension and other CKD symptoms, the search for effective drugs that can stimulate erythropoiesis in CKD and do not have nephrotoxic properties is particularly relevant. The aim of the work was to study the effectiveness of Gamavit in the treatment regimen for cats with CKD compared with the standard treatment protocol. Active ingredients of Gamavit are sodium nucleinate and denaturated placenta extract. The study included 20 cats of different breeds aged from 5 to 16 years with CKD stage III-IV according to the IRIS classification, accompanied by anemia and a decrease in hematocrit in the range from 25 to 16%. Patients were randomly divided into 2 equal groups - control (n=10) and experimental (n=10) using the block randomization method. In the control group a standard nephroprotective therapy regimen was used. In the experimental group Gamavit was added to the standard regimen. Gamavit was administered at a dose of 0.5 ml/kg 1 time per day, at the same time, during 14 consecutive days (7 days i/v and 7 days s/c). The conditions for feeding and keeping animals of the experimental and control groups were identical. For feeding, we used a standardized serial diet ― Purina CatChow Adult with chicken/turkey/salmon. Access to drinking water ― ad libitum. The initial number of red blood cells, as well as the hematocrit index and hemoglobin level in the experimental and control groups were at the lower limit of the norm or below the reference values. During the study, in the experimental group of the patients, these indicators returned to normal after 2 weeks, while in the control group there were no significant changes. The statistically significant difference between the experimental and control groups remained until the end of the observation (p=0.001). Levels of creatinine and urea in the blood serum of the patients of both groups consistently high throughout the study, but in the experimental group a significant decrease in these indicators was found. The initial level of EPO in both groups was significantly lower than in healthy cats. During the experiment, it was not possible to reach the lower limit of the normal value, which allows us to conclude that the stimulation of erythropoiesis indicators in the experimental group, observed under the influence of Gamavit, is not associated with the stimulation of endogenous EPO production. Thus, the study revealed that Gamavit stimulates erythropoiesis in cats with CKD stage III-IV, significantly increasing the number of red blood cells, and the levels of hematocrit and hemoglobin. This effect, combined with a decrease in uremic intoxication values (creatinine and urea levels), lead to an improvement in the general condition of patients and regression in a number of important clinical manifestations. Since no nephrotoxic effects and undesirable interactions with drugs of standard nephroprotective therapy were detected in the process of this study, Gamavit can be recommended for widespread use in cats as one of the pathogenetic, symptomatic, and therapeutic and prophylactic drugs in the complex therapy of CKD at the preclinical and clinical stages of the disease.

Study The Effect of Dietary Supplementation of Crushed Seeds of Coriander (Coriandrum Sativum) on Some Physiological Parameters of Rabbits.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sura Safi Obayes Khafaji
Keyword(s):  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Basal Diet ◽  
Experimental Period ◽  
Physiological Parameters ◽  
Coriandrum Sativum ◽  
Control Group ◽  
T1 And T2

The aim of present research to study the effects of crushed seeds of Coriander (Coriandrum sativum) on some physiological parameters in rabbits. Thirty mature male rabbits, (aged 87-100 days),were divided randomly into three equal groups. First group (control) fed the basal diet along the experimental period,2nd and 3rd groups (T1 and T2) fed the basal diet mixed with 3% and 6%,respectively. At the end of experiment,blood samples were collected from ear vein for counting the red blood cells (RBCs) and white blood cells (WBCs),estimating Hemoglobin concentration (Hb) and packed cell volume (PVC). Total protein,albumin,globulin,blood glucose,GOT and GPT concentrations were estimated in blood serum. The results showed significant increase (P<0.05) of RBCs,WBCs,Hb and PCV levels in T1 and T2 groups when compared with control group. As well as,The results of total protein and globulin increased significantly in two treated groups in comparison with control groups. While,the results of glucose,GOT and GPT levels showed a significant decrement (P<0.05) in T1 and T2 groups compared with control group. Therefore,it was concluded that the use of Coriander seeds powder can affected positively on some physiological traits in rabbits.

Imatinib mesylate has an inhibitory effect on hematopoiesis in a mice model

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 5568-5568
Author(s):  
Jie yu Ye ◽  
Su yi Li ◽  
En yu Liang ◽  
Fan yi Meng ◽  
Beng Chong ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Tyrosine Kinase ◽  
Imatinib Mesylate ◽  
Blood Cells ◽  
White Blood Cells ◽  
Mice Model ◽  
Cell Counts ◽  
Number Of Patients ◽  
And Control

Abstract Imatinib mesylate (Gleevec or STI-571), a tyrosine kinase inhibitor, can potently block both platelet-derived growth factor (PDGF) receptor-α and β by inhibiting the receptor tyrosine kinase activity. Imatinib mesylate has been successfully used to treat patients with chronic myeloid leukemia (CML) and Ph positive acute lymphoid leukemia (ALL). However, a significant number of patients with imatinib mesylate treatment have developed neutropenia, thrombocytopenia and anemia (van Deventer, Hall et al. 2002). Our previous studies have demonstrated that PDGF/PDGFR plays an important role on thrombopoiesis, and blockage of PDGFR by imatinib inhibited the downstream signal pathway, which led to reduce of megakaryocyte production (Ye, et al. Haematologica, 2010). This may partially explain the imatinib-related thrombocytopenia. However, the role of imatinib on normal hematopoiesis remains to be investigated. In the present study, we identified the expression of PDGFR-β on the human megakaryoblastic cells: Meg-01, CHRF-288-11, Dami and M-07e, human erythromyeloblastoid leukemia cells: K-562 and human myeloblastic cells: HL-60 by RT-PCR, western blot and flow cytometry. We then demonstrated the effect of imatinib on normal hematopoiesis in the 8 wk old C57BL/6 mice model. Gleevec (25 mg/kg/day) dissolved in distilled water or water only were given orally for up to 2 wks. 15ul whole blood was taken from postorbital weekly to perform blood cell counts. The Gleevec -treated group showed a reduction of platelet count as compared to the control at day 14 (543.3±71.95×109/L vs. 330.0±62.23×109/L, n=5, P<0.05). Similarly, Gleevec caused a significant decrease in the number of white blood cells on day 14 (9.667×±0.8511×109/L vs. 6.080±0.8015×109/L, n=5, P<0.05). While no significant differences were found on red blood cell count between Gleevec and control groups. The mice bone marrow was harvested from each group to perform CFU assays. Gleevec significantly inhibited the formation of bone marrow CFU-MK (15.33±1.764 vs 3.333±0.6667, n=3), and CFU-GM(13.50±2.784 vs 6.333±0.7265, n=3) compared with the control. There were no significant differences found in CFU-GEMM and CFU/BFU-E formation between the Gleevec and control treatment. Our studies suggested that PDGFR were expressed on hematopoietic cells. Imatinib mesylate, an inhibitor of PDGFR, not only played an important role in malignant hematopoiesis, but also in normal hematopoiesis. Administration of Gleevec reduced white blood cells and megakaryocytes/platelets production, which implied its potential therapeutic role in treating myeloproliferative diseases, such as Essential Thrombocytosis. Disclosures: No relevant conflicts of interest to declare.

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