Aktivitas Antibakteri Ekstrak Akar Ceplukan (Physalis angulata L.) Terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa

2017 ◽  
Author(s):  
Pratika Viogenta ◽  
Lilik Koernia Wahidah ◽  
Ika Harum Saputri
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Inhibitory Zone ◽  
Root Extracts ◽  
Physalis Angulata

Root ceplukan (Physalis angulata L.) is one of the medicinal plants that contain several active compounds that are antibacterial. This study aims to prove the antibacterial activity of root ceplukan extract againsts Staphylococcus epidermidis bacteria and Pseudomonas aeruginosa bacteria. Root ceplukan was extracted by maceration with ethanol 70%. Root root extracts of phytochemical screening include flavonoids, alkaloids and saponins. The ceplukan root extract was then tested against S. epidermidis and P. aeruginosa bacteria using wells method with concentrations of 20%, 40%, 60%, 80%, 100%, aquades as a negative control and amikacin as a positive control. Inhibitory zone diameter with each concentration for P. aeruginosa bacteria, among others, negative control = 0 mm, positive control = 21,623 mm, 100% = 16.9 mm, 80% = 15.05 mm, 60% = 13.703 mm, 40% = 12.02 mm, 20% = 12.02 mm and S. epidermidis bacteria have inhibitory zone at positive control of 21,623 mm. The results of this study showed that root extract ceplukan proved to have inhibition zone against bacteria P. aeruginosa at all concentrations but has no inhibition zone against S. epidermidis bacteria. Root ceplukan extract has a minimum inhibitory concentration of 14% and a minimum bacterisid concentration of 20%, against P. aeruginosa bacteria. The results of phytochemical analysis showed that root ceplukan positively contain flavonoids, alkaloids and saponins are suspected as antibacterial compounds. Keywords : Antibacterial, Staphylococcus epidermidis, Pseudomonas aeruginosa, Ceplukan root extract

THE EFFECTIVENESS TEST OF MATOA (PometiaPinnata) ENDOPHYTIC BACTERIA TOWARDS BACTERIA NOSOCOMIAL INFECTION

2020 ◽  
Vol 1 (1) ◽  
pp. 37-40
Author(s):  
Denny Chandra Halid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infection ◽  
Staphylococcus Epidermidis ◽  
Endophytic Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Isolates ◽  
Series Design ◽  
Inhibition Zones ◽  
The One

This study aims to determine the effectiveness of Matoa (Pometiapin-nata) endophytic bacteria towards bacteria nosocomial infection namely Pseudomonas aeruginosa and Staphylococcus epidermidis. The subjects in the study were Matoa plant endophytic bacterial isolates on the stem (tw-igs). The positive control used is meropenem & negative control of aquades. This type of research uses quasi-experiments with a research design us-ing the One-Group Time-Series Design. The result of the study shows that there are 2 endophytic bacterial iso-lates in Matoa plants namely BEM 1 and BEM 2. Both endophytic bacterial isolates can kill and inhibits bacterial nosocomial infections Pseudomonas aeruginosa and Staphylococcus epider-midis with inhibition zones in the range of 16mm-22mm with a strong category very strong that it has the po-tential to be used as an antibacterial

scholarly journals The Potential of Mango (Mangifera infica L.) Peel of Apple Varieties By Infusion And Maceration In Inhibiting Pseudomonas aeruginosa And Propionibacterium acnes

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Vifin Putri Rahmawati ◽  
Chylen Setiyo Rini
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chemical Components ◽  
Extract Concentration ◽  
Potential Test ◽  
Antibacterial Potential

Plants have many chemical components. The use of natural ingredients as an alternative treatment in dealing with diseases, especially acne. One of them is mango (Mangifera indica L.) varieties of apples obtained at the Larangan Main Market in Sidoarjo. This study aims to determine the potential of infusion and maceration of mango skin varieties in inhibiting Pseudomonas aeruginosa and Propionibactrium acne at various concentrations. This antibacterial potential test was carried out using the diffusion method of the wells. The antibacterial potential is characterized by the formation of a clear zone around the well called the inhibition zone. This study uses 10 concentrations namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% and Clindamycin as positive control and aquades as negative control. Based on the results of the Two Way ANOVA test data obtained were not normally distributed, therefore a comparison test was performed using the Kruskal-Wallis test with a sign value (α <0.05). This showed that there were significant differences in the use of various concentrations. The maceration extract concentration of 100% is the best concentration to form a zone of inhibition against P. aeruginosa  of 17.9 mm and P. acne bacteria of 13.2 mm. The results of the infusion extract concentration did not form inhibitory zones in both of P. aeruginosa and P. acnes.

scholarly journals Potensi Antibakteri Ekstrak Daging Buah Kelubi (Eleiodoxa conferta) Bangka Belitung Menggunakan Microwave-Assisted Extraction (MAE)

2020 ◽  
Vol 7 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Surtina Surtina ◽  
Ratih Puspita Sari ◽  
Zulita Zulita ◽  
Rani Rani ◽  
Occa Roanisca ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Microwave Assisted Extraction ◽  
Test Results ◽  
Inhibitory Zone ◽  
E Coli ◽  
Microwave Assisted ◽  
Assisted Extraction

Antibacterial tests were carried out using ethanol extract of kelubi meat (Eleidoxa conferta), extraction using the Microwave-Assisted Extraction method with a ratio of 2: 20 for 30 minutes at 60 °C. Antibacterial tests using diffusion methods with concentrations of 20%, 40%, 60%, 80%, and 100%; positive control of 0.005% amoxillin and DMSO negative control. Antibacterial test results showed that the inhibition zone of ethanol extract in E. coli bacteria with a concentration of 20% was in the moderate category, for a concentration of 40% including the strong category while the concentration of 60%, 80% and 100% was very strong. The inhibition zone of ethanol extract for the S. aureus bacterium with a concentration of 20% is included in the strong category while the concentration of 40%, 60%, 80%, and 100% is categorized as very strong. From these results it can be concluded that the inhibitory zone in Stapylococcus aureus bacteria is greater than that of E. coli bacteria.

scholarly journals Aktivitas Antibakteri Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa Secara In Vitro (ANTIBACTERIAL ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAF EXTRACT ON GROWTH OF BACTERIA PSEUDOMONAS AERUGINOSA IN VITRO)

2019 ◽  
Vol 20 (3) ◽  
pp. 384
Author(s):  
Faisal Fikri ◽  
Imas Hapsari Rahmaningtyas ◽  
Ragil Angga Prastiya ◽  
Muhammad Thohawi Elziyad Purnama
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacterial Growth ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Annona Muricata ◽  
Bacterial Growth Inhibition

The aim of this study was to measure antibacterial activity of soursop leaf extract (Annona muricata L.) on bacterial growth inhibition of Pseudomonas aeruginosa by diffuse disc method. The result of the inhibition zone was analyzed using Analysis of Variance and followed using Duncan test, to show highly significantly different (p<0.01). The inhibition number of negative control (C-) was 0.00e±0.00 and the positive control (C+) was 31.31a±0.57. The result of concentration 1 (T1) was 14.49b±4.96; concentration 2 (T2) 8.98c±1.29; concentration 3 (T3) 5.84cd±1.85; concentration 4 (T4) 5.56cd±1.58; concentration 5 (T5) 2.85de±0.28; concentration 6 (T6) 2.98de±0.53; concentration 7 (T7) 2.82de±1.59; concentration 8 (T8) 2.41de±1.10; concentration 9 (T9) 2.20de±0.34; concentration 10 (T10) 1.10e±0.19; and concentration 11 (T11) 0.00e±0.00. The increase of soursop leaf extract concentration showed high inhibition diameter of bacterial growth. It concluded that soursop leaf extract have an antibacterial activity to inhibit P. aeruginosa growth with Minimal Inhibitory Concentration (MIC) 125 ppm.

scholarly journals Effectiveness of Matoa Leaf (Pometia pinnata) Extract as an Antibacterial Staphylococcus epidermidis

2021 ◽  
Vol 3 (1) ◽  
pp. 1-8
Author(s):  
Rossalinda Rossalinda ◽  
Fitria Wijayanti ◽  
Damayanti Iskandar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Sample Concentration ◽  
Inhibition Zones

A natural alternative that can be used to inhibit the growth of Staphylococcus epidermidis bacteria is matoa leaves (Pometia pinnata). Matoa leaves contain secondary metabolites such as flavonoids, tanins, and saponins which are known as an antibacterial. The purpose of this study was to determine the effectiveness of matoa leaves extract as an antibacterial agent for Staphylococcus epidermidis. This study used the disc diffusion method, using deodorant as a positive control, aquadest as a negative control and concentration variations matoa leaves extract of 15%, 20%, 25% and 30%. The matoa leaves were extracted using the maceration method with 96% ethanol. Antibacterial testing in this study was characterized by a clear zone or zone of inhibition around the disc paper. The results of the antibacterial test in this study have to different inhibition zones, that is a sample concentration of 15% the inhibition zone value is 0,125 mm. The sample concentration of 20% the inhibition zone is 3 mm. The sample concentration of 25% the inhibition zone  is 2,312 mm and the sample concentrasion of 30% the inhibition zone is 0,875 mm. The extract of matoa leaves can be used as an antibacterial, because according to Pan Chen Wu Tang and zao (2009) the extract of matoa leaves could be applied as inhibitor of the growth of Staphylococcus epidermidis at a sample concentration of 20% is in the weak to moderate category

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

In vitro Anthelminthic Activity of Crude Methanolic Leaf Extract of Hyptis suaveolens (L.) Poit (Bush mint) on Ascaris suum.

2019 ◽  
Vol 2 (1) ◽  
pp. 82-87
Author(s):  
O. S Salami
Keyword(s):  
Leaf Extract ◽  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Analysis ◽  
Egg Hatchability ◽  
Anthelminthic Activity ◽  
Life Cycle Stages ◽  
Hyptis Suaveolens

Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.

Biogenic Synthesis of AgNPs with Saussurea lappa C.B. Clarke and Studies on Their Biochemical Properties

2018 ◽  
Vol 18 (12) ◽  
pp. 8392-8398 ◽  
Author(s):  
Muhammad Riaz ◽  
Muhammad Altaf ◽  
Amir Faisal ◽  
Muhammad Azhar Shekheli ◽  
Ghulam Abbass Miana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Xrd Analysis ◽  
Biochemical Properties ◽  
Root Extract ◽  
Biogenic Synthesis ◽  
Synthesis Of Nanoparticles ◽  
Saussurea Lappa ◽  
Drug Candidates

Nanotechnology has become an irreplaceable need and green synthesis of nanoparticles offers several advantages over physical and chemical methods. Medicinal plants are the main reservoirs of drugs and drug candidates. We report the biogenic synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Saussurea lappa. Verification and characterization of these nanoparticles were done by UV-visible spectroscopy, XRD-analysis and Scanning Electron Microscopy and FT-IR. Extract-loaded-AgNPs showed the highest inhibition zone against Escherichia coli (11.0 mm) and intermediate against Pseudomonas aeruginosa (9.0 mm). The methanolic root extract of S. lappa alone, also moderately inhibited Pseudomonas aeruginosa (9.0 mm) and showed lower activity (6.0 mm) against Escherichia coli. Its aqueous roots extract inhibited (6.0 mm) the growth of tested organisms. Methanolic extract showed antioxidant potency (IC50 = 0.814 μg/mL). Experiments revealed the presence of phenols and flavonoids in the roots of Saussurea lappa. These findings provide promising interest to exploit Saussurea lappa for the biogenic synthesis of AgNPs and their biological applications.

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