Investigation of the potential use of VCAM-1, TNF-α, IL-10 and IL-6 as biomarkers of nickel exposure

Nanobiotechnology strategies for cancer treatments are currently being tested with increasing interest, except in elderly groups. It is well established that breast cancer incidence increases with age and that traditional therapies usually generate severe adverse effects, especially for elderly groups. To investigate if the benefits of nanotechnology could be extended to treating cancer in this group, citrate-coated maghemite nanoparticles (NpCit) were used for magnetohyperthermia (MHT) in combination with the administration of PLGA-Selol nanocapsule (NcSel), a formulation with antioxidant and antitumor activity. The combined therapies significantly inhibited breast Ehrlich tumor growth and prevented metastases to the lymph nodes, liver and lungs until 45 days after tumor induction, a better result than the group undergoing conventional drug treatment. The levels of TNF-α, associated with poor prognosis in Ehrlich tumor, were also normalized. Therefore, the results evidenced the potential use of these therapies for future clinical trials in elderly breast cancer patients.

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Zinc-phosphate nanoparticles with reversibly attached TNF-α analogs: an interesting concept for potential use in active immunotherapy

Journal of Nanoparticle Research ◽

10.1007/s11051-010-0199-5 ◽

2011 ◽

Vol 13 (7) ◽

pp. 3019-3032 ◽

Cited By ~ 5

Author(s):

Gorazd Hribar ◽

Andrej Žnidaršič ◽

Marjan Bele ◽

Uroš Maver ◽

Simon Caserman ◽

...

Keyword(s):

Zinc Phosphate ◽

Active Immunotherapy ◽

Tnf Α ◽

Potential Use

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IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-γ production by CD25+Foxp3- T cells

eLife ◽

10.7554/elife.62432 ◽

2021 ◽

Vol 10 ◽

Author(s):

Jakub Tomala ◽

Petra Weberova ◽

Barbora Tomalova ◽

Zuzana Jiraskova Zakostelska ◽

Ladislav Sivak ◽

...

Keyword(s):

T Cells ◽

Autoimmune Diseases ◽

Pancreatic Islet ◽

Nude Mice ◽

Safety Issue ◽

Islet Allografts ◽

Tnf Α ◽

Ifn Γ ◽

Potential Use ◽

Higher Sensitivity

Complexes of IL-2 and JES6-1 mAb (IL-2/JES6) provide strong sustained IL-2 signal selective for CD25+ cells and thus they potently expand Treg cells. IL-2/JES6 are effective in the treatment of autoimmune diseases and in protecting against rejection of pancreatic islet allografts. However, we found that IL-2/JES6 also dramatically increase sensitivity to LPS-mediated shock in C57BL/6 mice. We demonstrate here that this phenomenon is dependent on endogenous IFN-γ and T cells, as it is not manifested in IFN-γ deficient and nude mice, respectively. Administration of IL-2/JES6 leads to the emergence of CD25+Foxp3-CD4+ and CD25+Foxp3-CD8+ T cells producing IFN-γ in various organs, particularly in the liver. IL-2/JES6 also increase counts of CD11b+CD14+ cells in the blood and the spleen with higher sensitivity to LPS in terms of TNF-α production and induce expression of CD25 in these cells. These findings indicate safety issue for potential use of IL-2/JES6 or similar IL-2-like immunotherapeutics.

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Abstract 646: The Existence of a Strong Correlation Between Leptin, TNF α Levels and BMI in Morbidly Obese Appalachian Females

Hypertension ◽

10.1161/hyp.64.suppl_1.646 ◽

2014 ◽

Vol 64 (suppl_1) ◽

Author(s):

Ellen Thompson ◽

Kathleen O'Hanlon ◽

Morghan Getty ◽

Komal Sodhi ◽

Eamonn Maher ◽

...

Keyword(s):

Odds Ratio ◽

Tumor Necrosis ◽

Linear Regression Analysis ◽

Inflammatory Biomarkers ◽

Morbidly Obese ◽

Tnf Α ◽

First Time ◽

The Relationship ◽

Potential Use ◽

Necrosis Factor

Introduction: Although BMI contributes to increased levels of adipokines, such as leptin, and tumor necrosis factor (TNFα) and to a decrease in adiponectin, few studies have examined the association between leptin, TNFα and body mass index (BMI) in Appalachian females. Methods: A total of 74 subjects from the Appalachian region were examined for the effect of BMI on the levels of leptin, TNF α , and adiponectin. Patients without overt cardiovascular disease were included. Linear regression analysis was used to analyze for changes in the relationship between BMI, leptin, TNFα and adiponectin. Nonlinear regression was used to determine the odds ratio and confidence intervals. Results: Serum TNFα and leptin levels were significantly increased in subjects with an increased BMI of 45-72 (9.9pg/ml ± 0.56 and 7.7pg/ml ± 0.6 respectively; p<0.05), compared to subjects with a BMI of 20-32. This was paralleled by increased levels of leptin, TNFα and IL-6. IL-6 was elevated in subjects with a BMI of 45-72 (4.83pg/ml ± 0.40; p<0.02). IL-6 was increased to a greater extent than leptin. In contrast, adiponectin levels were decreased (p<0.05) in subjects with a BMI of > 45-72 (6.63ng/ml ± 0.5; p<0.05), compared to subjects with a BMI of 20-32 (14.43ng/ml ± 0.32). Conclusion: Our study demonstrates for the first time the unique inflammatory biomarkers profile in morbidly obese females, even before diagnosis of coronary disease, highlighting the possible pathogenic potentials and opens the door for potential use of these biomarkers as a prognostic tool and a therapeutic target that can ameliorate obesity induced cardiovascular dysfunction.

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Potential Use ofPlectranthus amboinicusin the Treatment of Rheumatoid Arthritis

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem168 ◽

2010 ◽

Vol 7 (1) ◽

pp. 115-120 ◽

Cited By ~ 21

Author(s):

Jia-Ming Chang ◽

Chun-Ming Cheng ◽

Le-Mei Hung ◽

Yuh-Shan Chung ◽

Rey-Yuh Wu

Keyword(s):

Rheumatoid Arthritis ◽

Inflammatory Diseases ◽

Type Ii Collagen ◽

Pharmacological Action ◽

Action Mode ◽

Reactive Protein ◽

Tnf Α ◽

Plectranthus Amboinicus ◽

Potential Use ◽

Bovine Type

Plectranthus amboinicus(P. amboinicus) is a folk herb that is used to treat inflammatory diseases or swelling symptoms in Taiwan. We investigated therapeutic efficacy ofP. amboinicusin treating Rheumatoid Arthritis (RA) using collagen-induced arthritis animal model. Arthritis was induced in Lewis rats by immunization with bovine type II collagen. Serum anti-collagen IgG, IgM and C-reactive protein (CRP) were analyzed. To understand the inflammation condition of treated animals, production of TNF-α, IL-6 and IL-1β from peritoneal exudates cells (PEC) were also analyzed.P. amboinicussignificantly inhibited the footpad swelling and arthritic symptoms in collagen-induced arthritic rats, while the serum anti-collagen IgM and CRP levels were consistently decreased. The production of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were also decreased in the high dosage ofP. amboinicusgroup. Here, we demonstrate the potential anti-arthritic effect ofP. amboinicusfor treating RA, which might confer its anti-rheumatic activity. This differs the pharmacological action mode of indomethacin.

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Withania coagulansFruit Extract Reduces Oxidative Stress and Inflammation in Kidneys of Streptozotocin-Induced Diabetic Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/201436 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 13

Author(s):

Shreesh Ojha ◽

Juma Alkaabi ◽

Naheed Amir ◽

Azimullah Sheikh ◽

Ahmad Agil ◽

...

Keyword(s):

Oxidative Stress ◽

Marked Reduction ◽

Diabetic Rats ◽

Inflammatory Status ◽

Withania Coagulans ◽

Immunoregulatory Cytokines ◽

Tnf Α ◽

Kidney Hypertrophy ◽

Markers Of Oxidative Stress ◽

Potential Use

The present study was carried out to investigate the changes in oxidative and inflammatory status in streptozotocin-induced diabetic rat’s kidneys and serum following treatment withWithania coagulans, a popular herb of ethnomedicinal significance. The key markers of oxidative stress and inflammation such as inflammatory cytokines (IL-1β, IL-6, and TNF-α) and immunoregulatory cytokines (IL-4 and IFN-γ) were increased in kidneys along with significant hyperglycemia. However, treatment of four-month diabetic rats withWithania coagulans(10 mg/kg) for 3 weeks significantly attenuated hyperglycemia and reduced the levels of proinflammatory cytokines in kidneys. In addition,Withania coagulanstreatment restored the glutathione levels and inhibited lipid peroxidation along with marked reduction in kidney hypertrophy. The present study demonstrates thatWithania coagulanscorrects hyperglycemia and maintained antioxidant status and reduced the proinflammatory markers in kidneys, which may subsequently reduce the development and progression of renal injury in diabetes. The results of the present study are encouraging for its potential use to delay the onset and progression of diabetic renal complications. However, the translation of therapeutic efficacy in humans requires further studies.

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Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study

BioMed Research International ◽

10.1155/2021/7453000 ◽

2021 ◽

Vol 2021 ◽

pp. 1-17

Author(s):

Hadeel A. Al-Rawaf ◽

Ahmad H. Alghadir ◽

Sami A. Gabr

Keyword(s):

Glycemic Control ◽

Inflammatory Cytokines ◽

Roc Analysis ◽

Serum Levels ◽

Poor Glycemic Control ◽

Circulating Mirnas ◽

Diagnostic Biomarkers ◽

Specificity And Sensitivity ◽

Tnf Α ◽

Potential Use

Background. Cellular miRNAs are expressed in tissue fluids with sufficient amounts and were identified as potential molecular targets for studying the physiological mechanisms and correlations with many human diseases particularly diabetes. However, molecular-based changes among older adults with diabetes mellitus (DM) are rarely fully elucidated. Aim. This study is aimed at identifying circulating miRNAs, which hold the potential to serve as biomarkers for the immune-inflammatory changes in older T2D patients with moderate and poor glycemic control status. In addition, the association of both myokines and osteopontin (OPN) levels with circulating miRNAs was identified. Methods. A total of 80 subjects aged 20–80 years were invited during the period of October 2017–May 2018 to participate in this descriptive cross-sectional study. All subjects were diagnosed with T2D for more than 5 years. Subjects were grouped based on glycemic control (HbA1c values) into two groups: moderate glycemic control (>7-8% HbA1c, no = 30 ) and poor glycemic control (>8% HbA1c, no = 50 ), respectively. Diabetic control parameters, fasting blood sugar (FS), HbA1c, fasting insulin (IF), insulin resistance (IR), HOMA-IR, inflammatory cytokines (IL-6, IL-8, IL-18, IL-23, TNF-α, and CRP), osteopontin, and myokines (adropin and irisin) were estimated by colorimetric and immune ELISA assays, respectively. In addition, real-time RT-PCR analysis was performed to evaluate the expression of circulating miRNAs, miR-146a and miR-144, in the serum of all diabetic subjects. Results. In this study, T2D patients with poor glycemic control showed a significant increase in the serum levels of IL-6, IL-8, IL-18, IL-23, TNF-α, CRP, and OPN and a reduction in the levels of myokines, adropin and irisin, compared to patients with moderate glycemic control. The results obtained are significantly correlated with the severity of diabetes measured by HbA1c, FS, IF, and HOMA-IR. In addition, baseline expression of miR-146a is significantly reduced and miR-144 is significantly increased in T2D patients with poor glycemic control compared to those with moderate glycemic control. In all diabetic groups, the expression of miR-146a and miR-144 is significantly correlated with diabetic controls, inflammatory cytokines, myokines, and serum levels of OPN. Respective of gender, women with T2D showed more significant change in the expressed miRNAs, inflammatory cytokines, OPN, and serum myokine markers compared to men. ROC analysis identified AUC cutoff values of miR-146a, miR-144, adropin, irisin, and OPN expression levels with considerable specificity and sensitivity which recommends the potential use of adropin, irisin, and OPN as diagnostic biomarkers for diabetes with varying glycemic control status. Conclusion. In this study, molecular expression of certain microRNA species, such as miR-146a and miR-144, was identified and significantly associated with parameters of disease severity, HbA1c, inflammatory cytokines, myokines, and serum osteopontin in T2D patients with moderate and poor glycemic control. The AUC cutoff values of circulating miRNAs, miR-146a and miR-144; myokines, adropin and irisin; and serum OPN were significantly identified by ROC analysis which additionally recommends the potential use of these biomarkers, miR-146a, miR-144, adropin, irisin, and OPN, as diagnostic biomarkers with considerable specificity and sensitivity for diabetes in patients with varying glycemic control status.

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Potential use of TNF-α inhibitors in systemic sclerosis

Immunotherapy ◽

10.2217/imt.13.173 ◽

2014 ◽

Vol 6 (3) ◽

pp. 283-289 ◽

Cited By ~ 24

Author(s):

Giuseppe Murdaca ◽

Francesca Spanò ◽

Miriam Contatore ◽

Andrea Guastalla ◽

Francesco Puppo

Keyword(s):

Systemic Sclerosis ◽

Tnf Α ◽

Potential Use

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Immune Protection of a Helminth Protein in the DSS-Induced Colitis Model in Mice

Frontiers in Immunology ◽

10.3389/fimmu.2021.664998 ◽

2021 ◽

Vol 12 ◽

Author(s):

Shao Rong Long ◽

Ruo Dan Liu ◽

Deepak Vijaya Kumar ◽

Zhong Quan Wang ◽

Chien-Wen Su

Keyword(s):

Intestinal Inflammation ◽

Inflammatory Responses ◽

Therapeutic Potential ◽

Trichinella Spiralis ◽

Tnf Α ◽

Inflammatory Processes ◽

Inflammatory Bowel ◽

Potential Use ◽

Colitis Model

Inflammatory bowel disease (IBD) increases the risk of colorectal cancer, and it has the potential to diminish the quality of life. Recent clinical and experimental evidence demonstrate protective aspects of parasitic helminth infection against IBD. Reports have highlighted the potential use of helminths and their byproducts as potential treatment for IBD. In the current study, we studied the effect of a newborn larvae-specific serine protease from Trichinella spiralis (TsSp) on the host immune and inflammatory responses. A 49-kDa recombinant TsSp (rTsSp) was expressed in Escherichia coli BL21 (DE3) and purified. The cytotoxicity of rTsSp was analyzed. The immune protective effect of rTsSp was studied by using dextran sodium sulfate (DSS)-induced mouse colitis model. The result illustrated that rTsSp has no toxic effects on cells. We further demonstrated that administration of the rTsSp without the additional adjuvant before the induction of DSS-induced colitis reduced the severity of intestinal inflammation and the disease index; it suppressed macrophage infiltration, reduced TNF-α secretion, and induced IL-10 expression. Our findings suggest therapeutic potential of rTsSp on colitis by altering the effect of macrophages. Data also suggest immunotherapy with rTsSp holds promise for use as an additional strategy to positively modulate inflammatory processes involved in IBD.

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The growth of mica polytypes as studied by conventional and high-resolution TEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100074690 ◽

1983 ◽

Vol 41 ◽

pp. 174-177

Author(s):

A. Baronnet ◽

M. Amouric

Keyword(s):

Water Pressure ◽

Point Of View ◽

Temperature Fields ◽

Natural Occurrence ◽

Growth Mechanisms ◽

Long Period ◽

High Resolution Tem ◽

Layer Stacking ◽

Experimental Works ◽

Potential Use

The origin of mica polytypes has long been a challenging problem for crystal- lographers, mineralogists and petrologists. From the petrological point of view, interest in this field arose from the potential use of layer stacking data to furnish further informations about equilibrium and/or kinetic conditions prevailing during the crystallization of the widespread mica-bearing rocks. From the compilation of previous experimental works dealing with the occurrence domains of the various mica "polymorphs" (1Mr, 1M, 2M1, 2M2 and 3T) within water-pressure vs temperature fields, it became clear that most of these modifications should be considered as metastable for a fixed mica species. Furthermore, the natural occurrence of long-period (or complex) polytypes could not be accounted for by phase considerations. This highlighted the need of a more detailed kinetic approach of the problem and, in particular, of the role growth mechanisms of basal faces could play in this crystallographic phenomenon.

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