Nano-Biosensors Tech and IPM in Plant Protection to Respond to Climate Change Challenges in Morocco

2020 ◽  
pp. 114-129
Author(s):  
Wafaa Mokhtari ◽  
Mohamed Achouri ◽  
Abdellah Remah ◽  
Noureddine Chtaina ◽  
Hassan Boubaker
Keyword(s):  
Plant Pathogens ◽  
Ad Hoc ◽  
Plant Protection ◽  
Point Of Care ◽  
Integrated Approach ◽  
Control Measures ◽  
Special Focus ◽  
Green Beans ◽  
Trichoderma Spp ◽  
Diagnostic Sensitivity And Specificity

In this chapter, the authors introduce two research axes: Part A, nano-biosensors as ad-hoc technologies designed to meet plant diagnostic sensitivity and specificity needs at point of care, and Part B, the study of the interaction of drought and infection stresses in crops investigating bio-control potential antagonists in developing integrated approach (IPM) for disease control measures in crops system. The first part will be revising most used nano-biosensors in plant pathogens detection using different platforms in greenhouses, on-field, and during postharvest. A special focus will be on optical and voltametric immuno/DNA sensors application in plant protection. The last part will present case studies of using nanoparticles functionalized with antibody/DNA for detecting pathogenic Pseudomonas sp, mosaic viruses, Botrytis cinereal, and Fusarium mycotoxins (DON). The second part will be interpreting experimental results of a case study on evaluating bio-control efficacy of local Trichoderma spp. using root dips treatment in Fusarium solani-green beans pathosystem as a model.

scholarly journals Research focus and authorship trends over 40 years of publications by New Zealand Plant Protection

2016 ◽  
Vol 69 ◽  
pp. 39-47
Author(s):  
T.M. Stewart
Keyword(s):  
New Zealand ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Integrated Management ◽  
Control Measures ◽  
Weed Species ◽  
Disease Biology ◽  
Research Findings ◽  
Chemical Solutions ◽  
Over 40 Years

In total 3053 contributions consisting of papers research notes and poster abstracts to the New Zealand Plant Protection Society from 1976 to 2015 were analysed for research and authorship trends The mean number of authors per paper per year increased from 18 to 43 over this period and institutional collaboration was increasingly common Proportionally there was a steady decrease in weed contributions and an increase in those featuring plant pathogens Control measures shifted from largely chemical solutions for single weed species to more integrated management programmes The number of papers on weed pest or disease biology incidence spread and impact were fairly constant over this period The scope of research widened considerably from a largely pastoral and arable focus to include environmental weeds and border biosecurity Topical research findings and review papers highlight the responsiveness of the plant protection research community to emerging novel issues

Radar-aided CSO-control-criteria for an ecological approach

1997 ◽  
Vol 36 (8-9) ◽  
pp. 223-228
Author(s):  
A. Petruck ◽  
F. Sperling
Keyword(s):  
Critical Time ◽  
Integrated Approach ◽  
Control Measures ◽  
Real Time Control ◽  
Sewer System ◽  
Time Control ◽  
Physical Measurements ◽  
Combined Sewer ◽  
Receiving Stream ◽  
Control Criteria

The control strategy of a combined sewer system incorporating three stormwater storage tanks with overflows presented here attempts to consider all aspects of acute CSO effects. These are the hydraulic and the composition components as well as the time factor. The result is an integrated approach, which is not based on the classic emission view (i.e. reduction of volume), but on pollution criteria (i.e. possible harm to the biotic community). The aim is to reduce the exceeding of critical peak values of the CSO components at critical time intervals. Control decisions will be based on continuous measurements in the sewer system and in the receiving stream. Furthermore the measurements are carried out to determine the effects (both hydraulic and chemical) of particular CSO discharges in order to evolve the critical values for the project area. The chemical and physical measurements are accompanied by a biological monitoring programme. Macroinvertebrates are sampled upstream and downstream of outfalls and at a reference site. This allows the evaluation of the control measures on an ecological basis, and thus an assessment of the ecological potential of radar-aided real-time control of the combined sewer systems.

scholarly journals Microbial antagonists against plant pathogens in Iran: A review

2020 ◽  
Vol 5 (1) ◽  
pp. 404-440 ◽  
Author(s):  
Mehrdad Alizadeh ◽  
Yalda Vasebi ◽  
Naser Safaie
Keyword(s):  
Biological Control ◽  
Chemical Control ◽  
Plant Disease ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Agricultural Products ◽  
Control Measures ◽  
Wide Range ◽  
Plant Disease Management ◽  
Published Research

AbstractThe purpose of this article was to give a comprehensive review of the published research works on biological control of different fungal, bacterial, and nematode plant diseases in Iran from 1992 to 2018. Plant pathogens cause economical loss in many agricultural products in Iran. In an attempt to prevent these serious losses, chemical control measures have usually been applied to reduce diseases in farms, gardens, and greenhouses. In recent decades, using the biological control against plant diseases has been considered as a beneficial and alternative method to chemical control due to its potential in integrated plant disease management as well as the increasing yield in an eco-friendly manner. Based on the reported studies, various species of Trichoderma, Pseudomonas, and Bacillus were the most common biocontrol agents with the ability to control the wide range of plant pathogens in Iran from lab to the greenhouse and field conditions.

scholarly journals Fungicidal Efficacy of Drying Plant Oils in Green Beans against Bean Rust (Uromyces appendiculatus)

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 143
Author(s):  
Vera Breiing ◽  
Jennifer Hillmer ◽  
Christina Schmidt ◽  
Michael Petry ◽  
Brigitte Behrends ◽  
...  
Keyword(s):  
Unsaturated Fatty Acids ◽  
Plant Protection ◽  
Linseed Oil ◽  
Uromyces Appendiculatus ◽  
Plant Oils ◽  
Bean Rust ◽  
Green Beans ◽  
Plant Oil ◽  
Water Emulsion ◽  
Tung Oil

As biorationals, plant oils offer numerous advantages such as being natural products, with low ecotoxicological side effects, and high biodegradability. In particular, drying glyceride plant oils, which are rich in unsaturated fatty acids, might be promising candidates for a more sustainable approach in the discussion about plant protection and the environment. Based on this, we tested the protective and curative efficacy of an oil-in-water-emulsion preparation using drying plant oils (linseed oil, tung oil) and a semi-drying plant oil (rapeseed oil) separately and in different mixtures. Plant oils were tested in greenhouse experiments (in vivo) on green beans (Phaseolus vulgaris L.) against bean rust (Uromyces appendiculatus). We observed that a 2% oil concentration showed no or very low phytotoxic effects on green beans. Both tested drying oils showed a protective control ranging from 53–100% for linseed oil and 32–100% for tung oil. Longer time intervals of 6 days before inoculation (6dbi) were less effective than shorter intervals of 2dbi. Curative efficacies were lower with a maximum of 51% for both oils when applied 4 days past inoculation (4dpi) with the fungus. Furthermore, the results showed no systemic effects. These results underline the potential of drying plant oils as biorationals in sustainable plant protection strategies.

Occupational hazard in Malaysian traffic police: special focus on air pollutants

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Putri Anis Syahira Mohamad Jamil ◽  
Karmegam Karuppiah ◽  
Irniza Rasdi ◽  
Vivien How ◽  
Shamsul Bahri Mohd Tamrin ◽  
...  
Keyword(s):  
Occupational Injuries ◽  
Indicator System ◽  
Control Measures ◽  
Special Focus ◽  
Safety Measures ◽  
Traffic Police ◽  
Wide Range ◽  
And Control ◽  
Risk Occupation ◽  
High Risk Occupation

Abstract This paper provides a specific deliberation on occupational hazards confronted daily by Malaysian Traffic Police. Traffic police is a high-risk occupation that involves a wide range of tasks and, indirectly, faced with an equally wide variety of hazards at work namely, physical, biological, psychosocial, chemical, and ergonomic hazards. Thereupon, occupational injuries, diseases, and even death are common in the field. The objective of this paper is to collate and explain the major hazards of working as Malaysian traffic police especially in Point Duty Unit, their health effects, and control measures. There are many ways in which these hazards can be minimised by ensuring that sufficient safety measures are taken such as a wireless outdoor individual exposure indicator system for the traffic police. By having this system, air monitoring among traffic police may potentially be easier and accurate. Other methods of mitigating these unfortunate events are incorporated and addressed in this paper according to the duty and needs of traffic police.

scholarly journals The impact of bZIP Atf1ortholog global regulators in fungi

2021 ◽  
Author(s):  
Éva Leiter ◽  
Tamás Emri ◽  
Klaudia Pákozdi ◽  
László Hornok ◽  
István Pócsi
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Secondary Metabolite ◽  
Plant Pathogens ◽  
Leucine Zipper ◽  
Secondary Metabolite Production ◽  
Special Focus ◽  
Human Pathogens ◽  
Metabolite Production ◽  
The Impact

Abstract Regulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on the function of fungal orthologs of the Schizosaccharomyces pombe Atf1, Aspergillus nidulans AtfA, and Fusarium verticillioides FvAtfA, bZIP-type transcription factors with a special focus on pathogenic species. We demonstrate that fungal Atf1-AtfA-FvAtfA orthologs play an important role in vegetative growth, sexual and asexual development, stress response, secondary metabolite production, and virulence both in human pathogens, including Aspergillus fumigatus, Mucor circinelloides, Penicillium marneffei, and Cryptococcus neoformans and plant pathogens, like Fusarium ssp., Magnaporthe oryzae, Claviceps purpurea, Botrytis cinerea, and Verticillium dahliae. Key points • Atf1 orthologs play crucial role in the growth and development of fungi. • Atf1 orthologs orchestrate environmental stress response of fungi. • Secondary metabolite production and virulence are coordinated by Atf1 orthologs.

scholarly journals The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciąg ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

AbstractPseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

scholarly journals LAMP-based foldable microdevice platform for the rapid detection of Magnaporthe oryzae and Sarocladium oryzae in rice seed

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. K. Prasannakumar ◽  
P. Buela Parivallal ◽  
Devanna Pramesh ◽  
H. B. Mahesh ◽  
Edwin Raj
Keyword(s):  
Magnaporthe Oryzae ◽  
Plant Pathogens ◽  
Point Of Care ◽  
Lamp Assay ◽  
Rice Seed ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Rice Seeds ◽  
Template Dna ◽  
Assay Conditions

AbstractRice blast (caused by Magnaporthe oryzae) and sheath rot diseases (caused by Sarocladium oryzae) are the most predominant seed-borne pathogens of rice. The detection of both pathogens in rice seed is essential to avoid production losses. In the present study, a microdevice platform was designed, which works on the principles of loop-mediated isothermal amplification (LAMP) to detect M. oryzae and S. oryzae in rice seeds. Initially, a LAMP, polymerase chain reaction (PCR), quantitative PCR (qPCR), and helicase dependent amplification (HDA) assays were developed with primers, specifically targeting M. oryzae and S. oryzae genome. The LAMP assay was highly efficient and could detect the presence of M. oryzae and S. oryzae genome at a concentration down to 100 fg within 20 min at 60 °C. Further, the sensitivity of the LAMP, HDA, PCR, and qPCR assays were compared wherein; the LAMP assay was highly sensitive up to 100 fg of template DNA. Using the optimized LAMP assay conditions, a portable foldable microdevice platform was developed to detect M. oryzae and S. oryzae in rice seeds. The foldable microdevice assay was similar to that of conventional LAMP assay with respect to its sensitivity (up to 100 fg), rapidity (30 min), and specificity. This platform could serve as a prototype for developing on-field diagnostic kits to be used at the point of care centers for the rapid diagnosis of M. oryzae and S. oryzae in rice seeds. This is the first study to report a LAMP-based foldable microdevice platform to detect any plant pathogens.

scholarly journals Robust simulation of mineral precipitation–dissolution problems with variable mineral surface area

2021 ◽  
Vol 129 (1) ◽  
Author(s):  
Serge Kräutle ◽  
Jan Hodai ◽  
Peter Knabner
Keyword(s):  
Surface Area ◽  
Ad Hoc ◽  
Chemical Species ◽  
Special Focus ◽  
Proof Of Concept ◽  
Mineral Surface ◽  
Mineral Precipitation ◽  
Numerical Tests ◽  
Ill Posed ◽  
Mineral Surface Area

AbstractWe consider a macroscale model of transport and reaction of chemical species in a porous medium with a special focus on mineral precipitation–dissolution processes. In the literature, it is frequently proposed that the reaction rate should depend on the reactive mineral surface area, and so on the amount of mineral. We point out that a frequently used model is ill posed in the sense that it admits non-unique solutions. We investigate what consequences this non-uniqueness has on the numerical solution of the model. The main novelty in this article is our proposal of a certain substitution which removes the ill-posedness from the system and which leads to better numerical results than some “ad hoc methods.” We think that the proposed substitution is a rather elegant way to get rid of the non-uniqueness and the numerical difficulties and is much less technical than other ideas. As a proof of concept, we present some numerical tests and simulations for the new model.

scholarly journals Synergistic Antifungal Activity of Graphene Oxide and Fungicides against Fusarium Head Blight In Vitro and In Vivo

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2393
Author(s):  
Xiuping Wang ◽  
Fei Peng ◽  
Caihong Cheng ◽  
Lina Chen ◽  
Xuejuan Shi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plant Pathogens ◽  
Antimicrobial Agents ◽  
Plant Protection ◽  
Disease Incidence ◽  
Agricultural Science ◽  
Plant Diseases ◽  
Synergistic Activity

Plant pathogens constantly develop resistance to antimicrobial agents, and this poses great challenges to plant protection. Therefore, there is a pressing need to search for new antimicrobials. The combined use of antimicrobial agents with different antifungal mechanisms has been recognized as a promising approach to manage plant diseases. Graphene oxide (GO) is a newly emerging and highly promising antimicrobial agent against various plant pathogens in agricultural science. In this study, the inhibitory activity of GO combined with fungicides (Mancozeb, Cyproconazol and Difenoconazole) against Fusarium graminearum was investigated in vivo and in vitro. The results revealed that the combination of GO and fungicides has significant synergistic inhibitory effects on the mycelial growth, mycelial biomass and spore germination of F. graminearum relative to single fungicides. The magnitude of synergy was found to depend on the ratio of GO and fungicide in the composite. In field tests, GO–fungicides could significantly reduce the disease incidence and disease severity, exhibiting a significantly improved control efficacy on F. graminearum. The strong synergistic activity of GO with existing fungicides demonstrates the great application potential of GO in pest management.

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