Preliminary Phytochemical Screening of Leaves of Ocimum tenuiflorum

2021 ◽  
Vol 1 (30) ◽  
pp. 63-66
Author(s):  
Shweta Tyagi I.P.Pandey
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Fats And Oils ◽  
Phytochemical Screening ◽  
The People ◽  
Ocimum Tenuiflorum ◽  
Phytochemical Constituents ◽  
Clinical Drugs ◽  
Clinical Drug

Abstract-The medicinal plants are useful for healing and curing of human diseases. Over 55% of all modern clinical drugs are of natural product origin. The plant, Ocimum tenuiflorum is highly used by the people of whole world specially Indians to cure various disorders because of the presence of phytochemical constituents. Ocimum tenuiflorum plant is known to possess anticancer, antispasmodic, antiviral, insecticide, antiseptic, analgesic, antiinflammatory, antimicrobial, antistress, Immunomodulatory, hypotensive and antioxidant properties. The present study reveals that various secondary metabolites such as glycosides, alkaloids, flavonoids, phenols, terpenoids, tannins, saponins, antraquenone , fats and oils and steroids are present in different leaf extract of the plant. Keywords: Ocimum tenuiflorum , secondary metabolites, clinical drug

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 384
Author(s):  
Caroline Wijaya ◽  
Berna Elya ◽  
Arry Yanuar
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Cassia Fistula ◽  
Kinetic Assay ◽  
Tyrosinase Inhibitory Activity ◽  
Water Fraction ◽  
Ic50 Value ◽  
Phytochemical Constituents

Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition ofdopachrome formation resulting from the L-DOPA-tyrosinase reaction.Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistulaleaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract containedalkaloids, flavonoids, glycosides, phenols, and tannins.Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.

scholarly journals A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera

2019 ◽  
Vol 20 (2) ◽  
pp. 17-24
Author(s):  
Hesty Parbuntari ◽  
Sri Benti Etika ◽  
Melindra Mulia ◽  
Elfanny Delvia
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Light Exposure ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
West Sumatra ◽  
Ocimum Tenuiflorum ◽  
Qualitative Test

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.

scholarly journals SKRINING FITOKIMIA DAN PENETAPAN KADAR FENOL TOTAL PADA EKSTRAK DAUN NANGKA (Artocarpus heterophyllus), CEMPEDAK (Artocarpus integer), dan TARAP (Artocarpus odoratissimus) ASAL DESA PENGARON KABUPATEN BANJAR

2021 ◽  
Vol 4 (1) ◽  
pp. 95-102
Author(s):  
Muhammad Ikhwan Rizki ◽  
◽  
Nurlely Nurlely ◽  
Fadlilaturrahmah Fadlilaturrahmah ◽  
Ma’shumah Ma’shumah
Keyword(s):  
Abdominal Pain ◽  
Secondary Metabolites ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Artocarpus Heterophyllus ◽  
Phenol Group ◽  
The People ◽  
Leaf Powder

The leaves of jackfruit (Artocarpus heterophyllus), cempedak (Artocarpus integer), and tarap (Artocarpus odoratissimus) are used empirically by the people of Desa Pengaron, Banjar Regency for treat antimalarials, antidiabetic, abdominal pain, and cancer. The secondary metabolites of the phenol group are responsible for various plant activities. This study aims to determine of compounds and total phenol content in leaves of jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus). The leaf samples were oven-dried, then the leaf powder was extracted using ethanol. Phytochemical screening was carried out on the extract using reagents, and phenol content was determined using a spectrophotometer UV-Vis. The results showed that the extracts of leaves jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus) contained phenol, flavonoids, and tannins. Total phenol content of leaves A. heterophyllus, A. integer and A. odoratissimus were 13,174 ± 1,378 mgGAE / gram extract, 37,204 ± 2,202 mgGAE / gram extract, and 35,886 ± 0.890 mgGAE / gram extract.

Preliminary phytochemical screening, isolation and structural elucidation of chloroform leaf extracts of Maesa lanceolata

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Chloroform Extract ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Standard Procedures ◽  
Phytochemical Constituents ◽  
First Time

The aim of this study was to screen the phytochemical constituents, isolate and elucidate the structure of chloroform extracts from the leaves of Maesa lanceolata. The qualitative phytochemical analysis of the leaf extract of Maesa lanceolata was done following standard procedures and the tests revealed the presence of cardiac glycosides and saponins in the crude extracts. The chloroform extract of the leaves of M. lanceolata led to the isolation of one compound, C1. Structure determination was accomplished by means of spectroscopic methods (IR, 13C and 1H NMR). According to the spectral data (IR, 1HNMR, 13CNMR and DEPT), probably Myrsenene was identified which is reported for the first time from M. lanceolata.

scholarly journals Biolarvicidal activity of Rhizophora stylosa leaf extract against Aedes sp.

2021 ◽  
Vol 869 (1) ◽  
pp. 012062
Author(s):  
D Syahputra ◽  
S Karina ◽  
Irwan ◽  
S Agustina ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Research Focus ◽  
Rhizophora Stylosa ◽  
Marine Chemistry ◽  
Qualitative Test ◽  
Banda Aceh

Abstract The research focus on biolarvicidal activity of Rhizophora Stylosa leaf extract against Aedes sp. R. stylosa leaf was collected from Gampong Pande, Banda Aceh. This research was conducted at laboratory of Marine Chemistry and Fisheries Biotechnology, Marine and Fisheries Faculty, Universitas Syiah Kuala from Desember 2020 to March 2021. The objective of this study was to figure out the secondary metabolites content in the methanol extract of the mangrove leaf and its biolarvicidal activity against Aedes sp. larvae. The secondary metabolites were screened out using phytochemical screening. The extract was tested for its biolarvicidal activity against third instar larvae of the Aedes sp. mosquito at incubation’s time of 48 hours. The biolarvicidal activity is expressed in LC50 which was determined by probit analysis using SPSS. The results of qualitative test showed that the extract contained of flavonoids, saponins, triterpenoids and tannins. Based on the results of probit analysis, the LC50 value of the extract against Aedes sp. was 858.89 ppm.

scholarly journals Qualitative Phytochemical Screening, GCMS Studies and In-Vitro Anti-Oxidative Properties of Aqeuous Leaf Extract of Gnetum africanum

2020 ◽  
Vol 10 (1) ◽  
pp. 11-17
Author(s):  
A.S. Ezekwe ◽  
P.C. Ugwuezumba ◽  
P. Nwankpa ◽  
J.N. Egwurugwu ◽  
C.N. Ekweogu ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Standard Procedures ◽  
Anti Oxidant ◽  
Aqueous Leaf Extract ◽  
Dpph Scavenging

This study aimed at carrying out a qualitative phytochemical screening, GC-MS studies and in-vitro antioxidant properties of aqueous leaf extract of Gnetum africanum. The qualitative phytochemical screening of the aqueous leaf extract of Gnetum africanum was done using standard procedures and revealed the presence of terpenoids, saponins, tannins, steroids, flavonoids, alkaloids, cardiac glucosides and phenols. The GC-MS screening revealed the presence of 14 compounds, 6 out of the 14 compounds were most prominent. The compound with the highest percentage peak area was caffeine with peak area of 96.9%, followed by n-Hexadacanotic acid with peak area of 60.9%, 2-methoxy-4-vinylphenol with peak area of 55.9%, tetradacanoic acid with peak area of 50.3%, cyclopentaneundecanoic acid with peak area of 47.8% and 2-cyclo-penten-1-2-hydroxy with peak area of 43.6% respectively. In-vitro determination of antioxidant property of leaf extract of Gnetum africanum was done photometrically using 2,2-dyhenyl-l-picrylhydrazyl (DPPH) assay. The DPPH scavenging ability of the leaf extract (43.2, 60.5, 68.8, and 75.7) was statistically significant at p<0.05 when compared with the standard drug ascorbic acid (81.1, 82.6, 85.1, and 90.4) % at 10, 20, 30 and 40 mg/l. In conclusion, the leaf extract of Gnetum africanum is loaded with a host of important phytochemicals and has antioxidant properties which increase in potency with increase dose. Keywords: Phytochemical Screening, GCMS Studies, Anti-Oxidant, Gnetum africanum

scholarly journals Effectiveness of antioxidant and anticoligenase activity of 70% ethanolic extracts of Kemangi leaves (Ocimum Basilicum)

2019 ◽  
Vol 4 (4) ◽  
pp. 13-18
Author(s):  
Hanjaya ◽  
Ermi Girsang ◽  
Ali Napiah Nasution ◽  
Sukirman Lie
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Power Method ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Collagenase Inhibition

Free radicals are one form of reactive oxygen compounds, which are generally known as compounds that have unpaired electrons. Kemangi leaves are used as an aphrodisiac because they contain araginin which can strengthen sperm resistance and prevent infertility. Besides araginin, Kemangi leaves also contain other secondary metabolites such as essential oils, phytosterols, alkaloids, phenolic compounds, tannins, lignin, saponins, flavonoids, terpenoids and anthraquinones. Phytochemical screening results of 70% ethanol extract of kemangi herbs themselves showed the presence of secondary metabolites of flavonoids, saponins, tannins and triterpenoids / steroids. In this study, antioxidant activity was tested using the FRAP (Ferric Reducing Antioxidant Power) method, with concentrations of kemangi leaf extract and comparative compounds Euginol each concentration of 1000 μL/ml, 5000 μL/ml, 250 μL/ml, and 125 μL/ml, 62.50 μL/ml, and 31.25 μL/ml measured at 745 nm wavelength and collagenase inhibition test with ethanol extract of kemangi leaves with a comparison of eugenol compounds measured at a wavelength of 335 nm. The FRAP antioxidant activity results obtained based on IC50 Eugenol value of 261.36 μg/ml and Kemangi leaf extract at 111.32 μg/ml, antolagenagenase from Eugenol at 255.32 μg/ml and Kemangi leaf extract at 110.65 μ/ml. 

scholarly journals Phytochemical Screening of White Turi (Sesbania grandiflora (L.) Pers.) Leaves Extract in Various Extraction Methods

2021 ◽  
Vol 4 (1) ◽  
pp. 22-29
Author(s):  
Jamilatur Rohmah ◽  
Ida Agustini Saidi ◽  
Luthfiyah Rofidah ◽  
Fia Novitasari ◽  
Frida Amelia Margareta
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Extraction Method ◽  
Leaf Extract ◽  
Extraction Methods ◽  
Test Results ◽  
Phytochemical Screening ◽  
Sesbania Grandiflora

One of the Fabaceae family that has potential as medicinal plant is the white Turi plant (Sesbania grandiflora (L.) Pers.).The purpose of this study was to determine the content of secondary metabolites which can be extracted using a various extraction methods.The extraction method used are digestion, percolation, reflux, soxhlet, infusion, and decoction extraction. The Turi plants used come from Mojosari, Mojokerto.The results of the initial phytochemical screening of white Turi leaf extract (Sesbania grandiflora (L.) Pers.) Were extracts in six extraction methods containing alkaloids, saponins and tannins.In extracts using the digestion, soxhlet, and reflux method containing steroids.Meanwhile, the four extracts from the digestion, percolation, soxhlet, and reflux methods contain phenolic.And the infusion and decoction method extracts containing flavonoids and triterpenoids. The similarity of the phytochemical test results for each extract but with different intensity results.

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