Highland amphibians - Recalculation of data from 1990 to 2010 on the effects of extremely diluted thyroxine

Experiments on amphibian metamorphosis can vary considerably in duration. The authors had set themselves the task of defining a generally applicable pooling method for metamorphosis experiments. The problem of artificial differences in variability when comparing and pooling data from several experiments was approached by normalization with respect to time based on the development of both test and the control animals. The range from 0% to 100% over which the fraction of four-legged animals progresses in the course of an experiment is divided into 10%-intervals and the 10% reference points are mapped on a corresponding scale. Each measurement is then assigned to the point on the time scale to which it is closest. In this way each reference point is assigned a value giving the number or percentage of four-legged animals at that point on the scale. Subsequent analysis was then based on the individual values for the test and control groups that corresponded to the joint 10% reference point. Normalization respect to time was done on the assumption that differences in metamorphosis speed attributable to treatment would override differences in duration between experiments. The results of experiments performed over the course of two decades (1990 - 2010) on highland Rana temporaria treated with a homeopathically prepared high dilution of thyroxine (“30x”) are presented in full detail based on this normalization method. Differences found between treatment groups thus calculated were in line with those obtained with other pooling methods. Thyroxine 30x does slow down metamorphosis in inert highland amphibians. This was observed by five researchers in 20 sub-experiments, and it seems to be the most reliable bio-assay found in amphibian research on homeopathy so far. When experiments were performed with highland animals pretreated by hyperstimulation with molecular thyroxine, slowing down of metamorphosis was again observed (by three out of four researchers) in most of 10 sub-experiments.