scholarly journals QTL analysis for rice salinity tolerance and fine mapping of a candidate locus qSL7 for shoot length under salt stress

2019 ◽  
Vol 90 (2) ◽  
pp. 307-319 ◽  
Author(s):  
Noushin Jahan ◽  
Yu Zhang ◽  
Yang Lv ◽  
Mengqiu Song ◽  
Chunyan Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Qtl Analysis ◽  
Dry Weight ◽  
Shoot Length ◽  
Chromosome 7 ◽  
Rice Breeding ◽  
Fresh Weight ◽  
Candidate Locus ◽  
Positive Role

AbstractSalinity is one of the major abiotic stresses limiting rice production worldwide. Understanding the genetic basis of salinity tolerance is key for rice breeding. In this study, a recombinant inbred line (RIL) population derived from a super hybrid rice Liang–You–Pei–Jiu (LYP9) parents 93-11 and PA64s, exhibited variation in phenotypes including shoot length (SL), root length (RL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), and root dry weight (RDW) under 50 and 100 mM NaCl stress. QTL analysis identified a total of 38 QTLs for these 6 traits under two distinct concentrations of salt, distributing on chromosomes 1, 2, 3, 4, 5, 6, 7 and 10. A total of 21 QTLs were found in 6 stable loci. A novel major QTL, qSL7 for shoot length on chromosome 7 was identified in two distinct concentrations. A chromosome segment substitution line (CSSL) harboring the qSL7 locus from PA64s with 93-11 background was developed and exhibited higher SL value, higher K+ concentration, and lower Na+ concentration compared to 93-11. With BC5F2:3 derived from CSSL-qSL7/93-11, the qSL7 was fine mapped within a 252.9 kb region on chromosome 7 where 40 annotated genes located including, LOC_Os07g43530, which encodes a DNA-binding domain containing protein reported previously as a transcription factor playing a positive role in salt stress tolerance. Our study provides new genetic resources for improvement of salinity tolerance in rice breeding.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals Morphological and Molecular Characterization of Soybean (Glycine max L.) Genotypes undre Salt Stress

2021 ◽  
Vol 24 (2) ◽  
pp. 33-46
Author(s):  
MS Rahman ◽  
MA Malek ◽  
RM Emon ◽  
A Hannan ◽  
GHM Sagor
Keyword(s):  
Salt Stress ◽  
Glycine Max ◽  
Chlorophyll Content ◽  
Coefficient Of Variation ◽  
Dry Weight ◽  
Shoot Length ◽  
Group Method ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Glycine Max L

Three advanced lines (SB02, SB05, SB07) along with one tolerant (Lokon) and one susceptible check (Asswt) of soybean (Glycine max L.) were assessed for salt tolerance in terms of morpho-physiological traits and molecular markers (SSR). The experiment was conducted at seedling stage with four salinity treatments namely 0, 8, 12 and 16 dSm-1 following Completely Randomized design. All the genotypes displayed considerable reduction in their morphological traits, least affecting the tolerant one. None of the genotypes were survived at 12 and 16 dSm-1 stress condition. Among the lines tested, SB-02 and SB-05 were identified as salt tolerant at 8 dSm-1 based on salinity susceptibility index (SSI) scoring. These genotypes suffered less in reduction of leaf chlorophyll content (SPAD) and increase of Na+/K+ than the susceptible genotypes. For all the traits viz. shoot length, root length, total length, shoot fresh weiht, root fresh weight, shoot dry weight, root dry weight, total fresh weight, total dry weight, percent live leaves, chlorophyll content and Na+/K+ ratio, the phenotypic coefficient of variation (PCV) was higher than that of genotypic coefficient of variation (GCV). All the traits studied showed medium to high heritability ranging between 43.81% (SPAD) to 96.65% (shoot length). The genotypes were grouped into two clusters considering both Euclidian distance and Unweighted Pair Group Method with Arithmetic Mean analysis. Lokon, SB-02 and SB-05 are on the same cluster as tolerant, and SB-7 and Asset on the other as susceptible to salt stress. The molecular pattern using by SSR marker displayed an average number of 3.33 alleles per locus with PIC (Polymorphism Information Content) values ranged from 0.2688 (sat_655 and satt728) to 0.7680 (sat_210). The highest gene diversity was observed in sat_210 and satt237 and the lowest in sat_655 and satt728 with a mean diversity of 0.5733. The genotypes Lokon, SB-02 and SB-05 could be suggested as a potential germplasm source of QTL (Quantitative Trait Loci) analysis for the development of salt tolerant soybean variety. Ann. Bangladesh Agric. (2020) 24(2): 33-46

scholarly journals Assessment of salinity tolerance in tomato cultivars grown in Maharashtra, India.

2018 ◽  
Vol 7 (5) ◽  
pp. 2259
Author(s):  
Rupali Seth
Keyword(s):  
Salinity Tolerance ◽  
Root Length ◽  
Growth Parameters ◽  
Genotypic Variation ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Solanum Lycopersicum L ◽  
Tomato Cultivars

The present study was undertaken to assess the genotypic variation for salinity tolerance in five commercial cultivars of tomato (Solanum lycopersicum L.) grown in Maharashtra. Growth parameters such as shoot length, root length, fresh weight and dry weight were assessed at control, 50 mM and 100 mM NaCl with Hoagland’s solution. The shoot/root length and fresh/dry weight declined at 100 mM stress. Proline accumulated as a consequence of salt stress. On the basis of growth parameters and proline accumulation cultivars Abhinav and Rohini were tolerant, TO1389 and N2535 moderately tolerant and Naina sensitive towards salinity stress.

scholarly journals The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Principal Component ◽  
Dry Weight ◽  
Seed Priming ◽  
Nacl Stress ◽  
Future Research ◽  
Fresh Weight ◽  
Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

scholarly journals Effects of partial defoliation on the growth, ion relations and photosynthesis of Lycium chinense Mill. under salt stress

2015 ◽  
Vol 67 (4) ◽  
pp. 1185-1194 ◽  
Author(s):  
Yuan Guo ◽  
Qiong Yu ◽  
Xiaohui Feng ◽  
Zhixia Xie ◽  
Xiaojing Liu
Keyword(s):  
Salt Stress ◽  
Physiological Response ◽  
Electron Transport Rate ◽  
Dry Weight ◽  
Photochemical Quenching ◽  
Leaf Water Content ◽  
Lycium Chinense ◽  
Fresh Weight ◽  
Relative Growth ◽  
Ion Relations

In this study, we investigated the effects of artificial defoliation on the growth and physiological response of Lycium chinense Mill. to salt stress. Our results show that partial defoliation increases the plant relative growth rate, leaf water content and dry weight-based leaf Na+ content, and reduces the fresh weight-based leaf Na+ content under salt stress. In response to defoliation, the leaf Na+/Ca2+ and Na+/Mg2+ ratios were decreased, but the K+ content remained unchanged. The contents of ROS and MDA were decreased in defoliated plants. Net The photosynthetic rate (PN), stomatal conductance (gs), electron transport rate (ETR), actual photochemical quenching (?PSII) and photochemical quenching (qp) were enhanced by defoliation. Together, these findings indicate that partial defoliation mitigates the salt-induced growth inhibition and physiological damage in L. chinense.

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

scholarly journals Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hassan O. Shaikhaldein ◽  
Fahad Al-Qurainy ◽  
Mohammad Nadeem ◽  
Salim Khan ◽  
Mohamed Tarroum ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chlorophyll Content ◽  
Total Protein ◽  
Dry Weight ◽  
Shoot Length ◽  
Antioxidant Enzyme Activities ◽  
Fresh Weight ◽  
Wide Range ◽  
Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

scholarly journals Effect of Water Extract Originated from Different Part of Russian Knapweed (Acroptilon repens L.) on Growth of Purslane (Portulaca oleracea L.)

2012 ◽  
Vol 4 (2) ◽  
pp. 108-111
Author(s):  
Alireza PIRZAD ◽  
Mousa JAMALI ◽  
Mohammad Amin ZAREH ◽  
Fahime SHOKRANI
Keyword(s):  
Aerial Part ◽  
Water Extract ◽  
Dry Weight ◽  
Shoot Length ◽  
Portulaca Oleracea ◽  
Control Treatment ◽  
Fresh Weight ◽  
Extract Concentration ◽  
Russian Knapweed ◽  
Seedling Length

To evaluate probable allelopathic effect of water extract originated from different parts of russian knapweed (Acroptilon repens L.) on growth of purslane (Portulaca oleracea L.), a factorial experiment was conducted based on randomized complete block design with three replications at greenhouse condition in 2011. Treatments were water extract concentrations (0, 5, 10, 15 and 20 percent) obtained from different part of russian knapweed (aerial part, flower and root). Results showed the significant effect of extract type on seedling length, and extract concentration on shoot length, root length and seedling length. Interaction effect between extract type and concentration on the ratio of root/shoot length, seedling fresh weight, seedling dry weight and germination percentage was significant, too. Means comparison indicated that the longest root (3.55 cm), shoot (4.65 cm) and seedling (8.20 cm) were obtained from control treatment, reducing with higher concentration of extract. The longest shoot (6.95 cm) belonged to extract originated from russian knapweed flowers. The highest ratio of root/shoot length (0.73), seedling fresh weight (0.61g) and seedling dry weight (0.044 g) belonged to control treatment. In general, increasing of extract concentration caused in reduction of germination and seedling traits. Meanwhile, extracts of aerial part had more sever reducing effect than root and flower extracts.

scholarly journals Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage

2019 ◽  
Vol 11 (9) ◽  
pp. 2584 ◽  
Author(s):  
Hafiz Ahmed ◽  
Muhammad Sajjad ◽  
Mingju Li ◽  
Muhammad Azmat ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Selection Criteria ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Drought Tolerant ◽  
Selection For ◽  
Thermo Stability

Diminishing water resources as a result of excessive use of water for irrigation and climate change posture a severe global threat to food security. Herein, an experiment was conducted to determine the selection criteria for drought-tolerant bread wheat genotypes at the seedling stage using morphological and photosynthetic pigmentation-related traits. A panel of 105 wheat landraces, historical Pakistani varieties, and advance breeding lines were evaluated under normal and drought stress using factorial completely randomized design. The root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b were positively correlated among themselves under both normal and stress conditions. Hence, selection of any one of these traits enhances the performance of other traits. The shoot length was non-significant and negatively associated with all other studied characters except relative water content. The results suggested that selection for shoot length could not improve genetic gain for drought tolerance. Out of 10 principal components (PCs), the first three PCs were showed significant genetic variation under both conditions. The first three PCs showed 74.6% and 76% cumulative genetic variation under normal and drought conditions, respectively. Based on PCA, 10 drought-tolerant and five drought-susceptible genotypes were identified. Overall results suggested that selection for root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b at the seedling stage would improve genetic gain for drought tolerance. The outperforming genotypes under drought stress conditions can be useful in future wheat breeding programs, and early selection for the traits recommended in this study will be effective for developing high-yielding and drought-tolerant wheat varieties.

Physiological response of chickpea (Cicer arietinum L.) at early seedling stage under salt stress conditions

2019 ◽  
Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seedling Growth ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Tolerance Index ◽  
Early Seedling Growth ◽  
Early Seedling

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.

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