Effect of the Extraction Technique and Operational Conditions on the Recovery of Bioactive Compounds from Chestnut (Castanea sativa) Bur and Shell

2014 ◽  
Vol 49 (2) ◽  
pp. 267-277 ◽  
Author(s):  
Adela Fernández-Agulló ◽  
M. Sonia Freire ◽  
Gervasio Antorrena ◽  
José Alberto Pereira ◽  
Julia González-Álvarez
Keyword(s):  
Bioactive Compounds ◽  
Castanea Sativa ◽  
Extraction Technique ◽  
Operational Conditions

scholarly journals Extraction Methods and Their Influence on Yield When Extracting Thermo-Vacuum-Modified Chestnut Wood

Forests ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Maurizio D’Auria ◽  
Marisabel Mecca ◽  
Maria Roberta Bruno ◽  
Luigi Todaro
Keyword(s):  
Catalyst Support ◽  
Castanea Sativa ◽  
Extraction Methods ◽  
Extraction Technique ◽  
Extraction Techniques ◽  
Surface Color ◽  
Ms Analysis ◽  
Castanea Sativa Mill ◽  
Molybdenum Catalysts ◽  
Chestnut Wood

Improvements in the yield and solubility of chestnut wood extractives, by using different extraction methods and molybdenum catalysts as support, have rarely been reported in literature. Many studies focus on the different parts of trees, except for the chemical characteristics of the remaining extractives achieved from thermally modified (THM) chestnut (Castanea sativa Mill) wood. This research seeks to better understand the effects of extraction techniques and catalysts on the yield and solubility of extractives. GC-MS analysis of the chloroform soluble and insoluble fractions was also used. Accelerated Solvent Extraction (ASE) 110 °C, Soxhlet, and autoclave extraction techniques were used to obtain extractives from untreated and thermally modified (THM) chestnut wood (170 °C for 3 h). Ethanol/H2O, ethanol/toluene, and water were the solvents used for each technique. A polyoxometalate compound (H3PMo12O40) and MoO3 supported on silica were used as catalysts. The THM induced a change in the wood’s surface color (ΔE = 21.5) and an increase in mass loss (5.9%), while the equilibrium moisture content (EMC) was reduced by 17.4% compared to the control wood. The yields of the extractives and their solubility were always higher in THM and mainly used ASE as the technique. GC-MS analysis of the extractives, without catalyst support, showed different results for each extraction technique and type of wood (untreated and THM). Ultimately, the amount of extractive compound dissolved in each solvent will differ, and the choice of extraction technique will depend on the intended final application of the extracted chemical product.

scholarly journals Cold Steeping Infusion, a Novel Lectin Extraction Technique for the Isolation, Purification and Partial Characterization of Lectins from the Green Venezuelan Marine Alga Caulerpa serrulata

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Pablo Djabayan-Djibeyan ◽  
Brian Carpenter ◽  
Gerardo Medina-Ramírez ◽  
Felix Andueza-Leal ◽  
Andrés León-Leal ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Red Blood Cells ◽  
Ammonium Sulfate ◽  
Bioactive Compounds ◽  
Blood Cells ◽  
Marine Alga ◽  
Continuous Production ◽  
Isotonic Saline ◽  
Size Exclusion ◽  
Extraction Technique

A lectin from the green Venezuelan marine alga Caulerpa serrulata was extracted with phosphate buffered saline (PBS) using cold steeping infusion (CSI) and by grinding with liquid nitrogen (GLN). The proteins were precipitated using solid ammonium sulfate. Both the crude extracts and ammonium sulfate precipitated proteins were tested for hemagglutinins using native and papain-treated human red blood cell suspensions in isotonic saline solution. Purification of lectins was achieved using affinity chromatography-sugar-epoxy-sepharose 6B and molecular weight was assessed by size exclusion chromatography using Bio-gel® P-100 and SDS-PAGE with 2-mercaptoethanol. IEF-urea 8M was also evaluated. Using CSI it was shown that the marine alga released hemagglutinating compounds into the solutions; the same hemagglutinating compounds were also obtained by GLN. Ammonium sulfate precipitated proteins exhibited agglutinating activity against native and papain-treated human red blood cells. Temperature and EDTA were shown to affect dramatically the lectin activity towards red blood cells. A lectin was purified efficiently and the molecular weight calculated as approximately 78,000 Daltons. The CSI technique demonstrated that the alga could be returned to an active metabolic state by immersion in a simple buffer after having been kept dormant by freezing at −20°C for long periods. It was also shown that the alga was releasing bioactive compounds into the solutions and, therefore, this procedure is being suggested as a good, gentle, non-disruptive extraction technique and we postulate CSI as a possible bioreactor for the continuous production of bioactive compounds from green marine algae.

scholarly journals Optimization of osmotic dehydration of chestnut (Castanea sativa Mill.) slices using Response Surface Methodology

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Teresa Delgado ◽  
Bruna Paim ◽  
José Alberto Pereira ◽  
Susana Casal ◽  
Elsa Ramalhosa
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Water Loss ◽  
Osmotic Dehydration ◽  
Sucrose Concentration ◽  
Volume Ratio ◽  
Castanea Sativa ◽  
Color Variation ◽  
Process Conditions ◽  
Operational Conditions

Osmotic dehydration of chestnut slices in sucrose was optimized for the first time by Response Surface Methodology (RSM). Experiments were planned according to a three-factor central composite design (α=1.68), studying the influence of sucrose concentration, temperature and time, on the following parameters: volume ratio, water activity, color variation, weight reduction, solids gain, water loss and normalized moisture content, as well as total moisture, ash and fat contents. The experimental data was adequately fitted into second-order polynomial models with coefficients of determination (R2) from 0.716 to 0.976, adjusted-R2 values from 0.460 to 0.954, and non-significant lacks of fit. The optimal osmotic dehydration process conditions for maximum water loss and minimum solids gain and color variation were determined by the “Response Optimizer” option: 83% sucrose concentration, 20 °C and 9.2 hours. Thus, the best operational conditions corresponded to high sugar concentration and low temperature, improving energy saving and decreasing the process costs.

scholarly journals Experimental Design and Optimization of Recovering Bioactive Compounds from Chlorella vulgaris through Conventional Extraction

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 29
Author(s):  
Ioulia Georgiopoulou ◽  
Soultana Tzima ◽  
Georgia D. Pappa ◽  
Vasiliki Louli ◽  
Epaminondas Voutsas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Chlorella Vulgaris ◽  
High Performance ◽  
Reversed Phase ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Operational Conditions ◽  
Design And Optimization ◽  
Conventional Extraction

Microalgae contain an abundance of valuable bioactive compounds such as chlorophylls, carotenoids, and phenolics and, consequently, present great commercial interest. The aim of this work is the study and optimization of recovering the aforementioned components from the microalgae species Chlorella vulgaris through conventional extraction in a laboratory-scale apparatus using a “green” mixture of ethanol/water 90/10 v/v. The effect of three operational conditions—namely, temperature (30–60 °C), duration (6–24 h) and solvent-to-biomass ratio (20–90 mLsolv/gbiom), was examined regarding the extracts’ yield (gravimetrically), antioxidant activity, phenolic, chlorophyll, and carotenoid contents (spectrophotometric assays), as well as concentration in key carotenoids, i.e., astaxanthin, lutein, and β-carotene (reversed-phase–high-performance liquid chromatography (RP–HPLC)). For this purpose, a face-centered central composite design (FC-CCD) was employed. Data analysis resulted in the optimal extraction conditions of 30 °C, for 24 h with 37 mLsolv/gbiom and validation of the predicted models led to 15.39% w/w yield, 52.58 mgextr/mgDPPH (IC50) antioxidant activity, total phenolic, chlorophyll, and carotenoid content of 18.23, 53.47 and 9.92 mg/gextr, respectively, and the total sum of key carotenoids equal to 4.12 mg/gextr. The experimental data and predicted results were considered comparable, and consequently, the corresponding regression models were sufficiently reliable for prediction.

scholarly journals Antioxidant Potential of Chestnut (Castanea sativa L.) and Almond (Prunus dulcis L.) By-products

2010 ◽  
Vol 16 (3) ◽  
pp. 209-216 ◽  
Author(s):  
J.C.M. Barreira ◽  
I.C.F.R. Ferreira ◽  
M.B.P.P. Oliveira ◽  
J.A. Pereira
Keyword(s):  
Lipid Peroxidation ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Castanea Sativa ◽  
Thiobarbituric Acid ◽  
Waste Products ◽  
By Products

The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC50 values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results.

scholarly journals Supercritical Carbon Dioxide Extraction of Polyphenols from Pomegranate (Punica granatum L.) Leaves: Chemical Composition, Economic Evaluation and Chemometric Approach

2012 ◽  
Vol 1 (3) ◽  
pp. 282 ◽  
Author(s):  
Rodrigo N. Cavalcanti ◽  
Helmut J. Navarro-Díaz ◽  
Diego T. Santos ◽  
Mauricio A. Rostagno ◽  
M. Angela A. Meireles
Keyword(s):  
Bioactive Compounds ◽  
Waste Treatment ◽  
Principal Component ◽  
Punica Granatum ◽  
Antimicrobial Activities ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Operational Conditions ◽  
Viable Solution ◽  
Increasing Demand

<p>The increasing demand for high-quality products and economically and environmentally friendly technologies, as well as restrictive legislative actions, has stimulated scientific research on the extraction, purification and identification of bioactive compounds from natural sources. Pomegranate (<em>Punica granatum </em>L.) is commonly used in traditional medicine due to its pharmacological properties, such as its anti-inflammatory, antihepatotoxicity, anti-lipoperoxidation, antidiabetic, anti-cancer and antimicrobial activities. The use of industrial residues as sources of bioactive compounds has emerged as an economically viable solution to the problem of solid waste treatment. In this context, this work aimed to evaluate the SC-CO<sub>2</sub> extraction of polyphenols from pomegranate leaves, evaluating the influence of temperature (40 and 50°C) and pressure (10-30 MPa) on extraction yield (EY), total phenolic content (TPC), antioxidant activity (AA) and the cost of manufacturing (COM) of the extracts. Principal component analysis (PCA) was used to reduce the dimensionality of multivariate data, making the visualization more straightforward and manageable. A high EY and TPC and low COM were obtained at the most effective operational conditions of 50°C and 30 MPa. The lack of correlation between EY-AA and TPC-AA indicated the coextraction of non-phenolic compounds. This assumption was corroborated by GC-MS analysis, which showed high levels of eicosanol, squalene, linoleic acid and tocols. Even though SC-CO<sub>2</sub> extraction resulted in a high TPC (257-389 mg.g<sup>-1</sup>) compared to the literature data, the low EY (0.21-0.67 %) and non-phenolic presence suggest that SC-CO<sub>2</sub> extraction may be a good purification pretreatment for the removal of non-polyphenolic compounds prior to further polyphenol extraction.</p>

scholarly journals Phytochemicals from Castanea spp. buds and green extraction technologies: the Finnover project

Italus Hortus ◽  
2020 ◽  
Vol 27 ◽  
pp. 19-29
Author(s):  
Dario Donno ◽  
Raffaella Boggia ◽  
Maddalena Guido ◽  
Maria Gabriella Mellano ◽  
Gabriele L. Beccaro
Keyword(s):  
Supply Chains ◽  
Bioactive Compounds ◽  
Sustainable Use ◽  
Value Added ◽  
Castanea Sativa ◽  
Bioactive Compound ◽  
Green Economy ◽  
Ultrasound Assisted Extraction ◽  
Green Extraction ◽  
Herbal Preparations

Green economy is a sustainable development tool based on the valorization of economic, natural and social resources. It is recognized as a tool to be applied to all the production sectors (goods and services), as well as for the conservation and sustainable use of natural resources. The FINNOVER project (“Innovative strategies for the development of cross-border green supply chains”) proposes a technical-economic path for the creation and development of new supply chains for the eco-sustainable extraction and use of natural bioactive compounds. In this study, an innovative extraction and re-use strategy to obtain value-added products from botanical by-products was developed as an alternative to waste incineration or composting. It was applied to Castanea sativa bud extract production as a case study, but it could be analogously applied for other herbal preparations. Castanea spp. and their preparations have been widely used for hundreds of years as medicinal plants in composite formulae. Bioactive compounds (botanicals) are quite variable in the plant material, according to genotype (intraspecific chemodiversity), different collection stages, pedoclimatic conditions of sampling sites (wild or cultivation zones), agrotechniques, and post-harvest handling. This research aimed to compare the bioactive compound pattern of Castanea spp. bud preparations (herbal preparations derived from embryonic fresh plant tissues as buds and sprouts) with the composition of extracts derived from the bud-waste management process. Molecules were extracted by the encoded traditional method (maceration in hydroglyceroalcoholic solution) and by green extraction technologies (Pulsed Ultrasound-Assisted Extraction). HPLC methods were used to identify and quantify the main bioactive compounds, and to obtain a specific profile to assess the contribution of every single bioactive class to the total phytocomplex. The established protocol was simple, sensitive and reliable and it could be used for the evaluation and quality control of natural products and relative eco-sustainable extracts. The valorization of bud marcs, which remain after the bud-preparation production, could have a significant economic impact for the commercial producers, representing an important innovation in this sector.

scholarly journals Synthesis of Starch Nanoparticles and Their Applications for Bioactive Compound Encapsulation

2021 ◽  
Vol 11 (10) ◽  
pp. 4547
Author(s):  
Diana Morán ◽  
Gemma Gutiérrez ◽  
María Carmen Blanco-López ◽  
Ali Marefati ◽  
Marilyn Rayner ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Surface ◽  
Synthesis Method ◽  
Future Research ◽  
Synthesis Methods ◽  
General Description ◽  
Operational Conditions ◽  
Starch Nanoparticles

In recent years, starch nanoparticles (SNPs) have attracted growing attention due to their unique properties as a sustainable alternative to common nanomaterials since they are natural, renewable and biodegradable. SNPs can be obtained by the breakdown of starch granules through different techniques which include both physical and chemical methods. The final properties of the SNPs are strongly influenced by the synthesis method used as well as the operational conditions, where a controlled and monodispersed size is crucial for certain bioapplications. SNPs are considered to be a good vehicle to improve the controlled release of many bioactive compounds in different research fields due to their high biocompatibility, potential functionalization, and high surface/volume ratio. Their applications are frequently found in medicine, cosmetics, biotechnology, or the food industry, among others. Both the encapsulation properties as well as the releasing processes of the bioactive compounds are highly influenced by the size of the SNPs. In this review, a general description of the different types of SNPs (whole and hollow) synthesis methods is provided as well as on different techniques for encapsulating bioactive compounds, including direct and indirect methods, with application in several fields. Starches from different botanical sources and different bioactive compounds are compared with respect to the efficacy in vitro and in vivo. Applications and future research trends on SNPs synthesis have been included and discussed.

Sign in / Sign up

Export Citation Format

Share Document