scholarly journals Effect of Level of Concentrate Feeding and Addition of Monensin on Blood Parameters of Awassi Lambs

2021 ◽  
Vol 910 (1) ◽  
pp. 012072
Author(s):  
Hayder F. H. Al-Shemary ◽  
Ali A. Saeed
Keyword(s):  
Body Weight ◽  
Wheat Straw ◽  
Total Protein ◽  
Blood Concentration ◽  
Blood Parameters ◽  
Diurnal Changes ◽  
Initial Weight ◽  
Blood Samples ◽  
Ground Wheat ◽  
Awassi Lambs

Abstract This study was conducted to investigate the effect of level of concentrate feeding and addition of monensin on blood parameters. Sixteen Awassi lambs were used at 4-6 months of age and mean initial weight of 21.27 kg. Concentrate diet was offered at two levels (2.5 and 3% of body weight, BW) with or without the addition of monensin at a rate of 30 mg/kg DM. Ground wheat straw was offered adlibitum. Results showed that increasing level of concentrate significantly increased (P<0.01) blood concentration of total protein (BTP) from 6.94 to 8.84 g.100 ml-1, urea nitrogen (BUN), and triglyceride (TG) from 15.54 to 18.23 and 22.66 to 25.12 mg.100 ml-1 respectively. The addition of monensin significantly increased (P<0.05) TP concentration from 7.61 to 8.17 g.100 ml-1, whereas, BUN concentration was decreased (P<0.01) from 18.15 to 15.62 mg.100 ml-1. All blood parameters were also affected (P<0.05) by the interaction between the level of concentrate and the addition of monensin. In the study of diurnal changes, blood parameters showed an expected response to the time of withdrawing blood samples from lambs.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

Effect of Tylvalosin on Blood Parameters in Healthy Broiler Chickens

2021 ◽  
Vol 19 (8) ◽  
pp. 141-144
Author(s):  
Mohammed Mosleh Shwaish ◽  
Ammar H. Salman ◽  
Asif Hasan Abdulrazaq
Keyword(s):  
Body Weight ◽  
Normal Saline ◽  
Broiler Chickens ◽  
Treated Group ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Blood Samples

To evaluate the effects of Tylvalosin on blood parameters in healthy broiler chickens, twenty broiler chicks (Ross 308) aged 21 days old and weight 600-800 gm was divided into two equal groups, the first one was control group (G1) and the second one was treated group (G2). G1 dosed with 1ml/kg of body weight of normal saline while G2 dosed with 25mg/Kg of body weight of Tylvalosin. Blood samples were collected with EDTA tubes after 4 days of treatment. The results showed significant decrease in total WBCs counts in G2 treated group as compared with control group (G1), also the eosinophil showed a significant decrease in G2 as compared with G1, while other parameters didn’t show any significant differences. In conclusion, WBCs were decreased after treatment with tylvalosin.

scholarly journals Use of probiotics instead of antibiotics in broiler production

2019 ◽  
Vol 29 (4) ◽  
pp. 359-370
Author(s):  
MJ Alam ◽  
Z Ferdaushi
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Growth Performance ◽  
Total Protein ◽  
Blood Parameters ◽  
Abdominal Fat ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Finisher Diets

Effects of different available commercial probiotics on growth performance and blood parameters were evaluated. To achieve the objectives, a total of 198 one-day-old Hubbard Isa Starbro broiler chicks were raised over 28 days. Chicks were wing-banded, weighed individually and randomly allocated into six equally major groups each having three replicates. Broilers of group T1 (control group) were fed the starter and finisher diets. The broilers of groups T2 was fed the control starter and finisher diets supplemented with antibiotic and groups T3-T5 were fed 4 different commercially available probiotics (T3-Guardizen-M, T4-Protexin and T5-Poultry star sol in drinking water. Weekly body weight, feed consumption and feed conversion were recorded during experimental period. Blood parameters at 4 weeks of age including packed cell volume (PCV), haemoglobin (Hb), total protein, albumin, triglycerides, high density lipoprotein (HDL), uric acid, cholesterol, glucose, intestinal microflora, pH and color properties of meat were determined. All birds were kept under similar environmental, managerial and hygienic conditions. Probiotic supplementation significantly increased the body weight and daily weight gain of broiler chicks at 28 days (p<0.05). Improved feed conversion was noticed in birds fed a diet supplemented with probiotic. The effects of probiotics on carcass and some internal organs were measured and results shows that feeding broilers with probiotics have significant effects (P < 0.05) on dressed carcass weight, abdominal fat, breast, thigh and liver while it appeared insignificant on gizzard (P> 0.05). The lower percent of abdominal fat and the higher percent of dressed carcass, breast and thigh were observed in experimental probiotic(s) groups. pH and meat color did not affect among treatments. There was lower mortality rate in probiotics among groups. Moreover, there was no significant change for Hb, PCV, total protein and albumin concentrations among different groups. Also, total protein, lipids and albumin concentrations were not affected by probiotic(s) supplementation. In addition, different probiotics showed a significant decrease (p<0.05) in triglycerides, cholesterol and uric acid concentration compared to control group. Birds supplemented with probiotics had higher number of lactobacilli but lower number of colibacilli compared to the control. It can be concluded that use of selected commercial probiotic(s) resulted in improved growth performance and carcass yields, and reduced serum cholesterol and uric acid in broiler chickens. Moreover, supplementation of the probiotic(s) to broilers had no detrimental effect on their growth performance and blood parameters. Therefore, usage of these probiotic(s) bacteria as antibiotic alternatives in poultry nutrition can be recommended. Progressive Agriculture 29 (4): 359-370, 2018

scholarly journals Effect of Varying Doses of Estradiol Benzoate on the body weight and blood picture in Turkeys

2012 ◽  
Vol 36 (2) ◽  
pp. 174-179
Author(s):  
Saad J. Gatie, Bassam A. Al-Shimmary
Keyword(s):  
Body Weight ◽  
Estradiol Benzoate ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Blood Picture ◽  
Blood Samples ◽  
The Third ◽  
Control Body ◽  
Rbc Count

A present study was carried out to investigate the effects of Estradiol benzoate 2 (mg/ml) hormone doses on the body weight and blood picture of Turkeys birds through studying the following parameters: body weight, RBC count , total WBC, MCV, MCH, MCHC, and platelet PLT. Forty growing Turkeys (30 days age) weighting between (250-350)gm were randomly divided into four groups (ten each group) treated as follows: Turkeys in the first group were injected with single dose of Estradiol benzoate (1 mg) on the first day of experiment (30 day post hatching) subcutaneously in the neck and termed as G1 group, the Turkeys in the 2nd group (G2) were injected Estradiol benzoate (2 mg) on the first day of experiment subcutaneously in the neck while the Turkeys in the third group (G3) were injected Estradiol benzoate (0.3 ml) at the first day of experiment subcutaneously in the neck the fourth group (G4) served as control, body weight was determined on (0, 15 and 30) days of experiment and blood samples were collected on 15th and 30th day of the experiment from groups to evaluate the blood parameters. Results revealed a significant increase (P<0.05) in body weight of G1, G2 and G3 in a comparison with control group at (15 and 30) days of experiment. Furthermore, in comparison among treated groups G2 and G3 also showed a significant increase (P<0.05) in the body weight as compared with G1 group during periods. Moreover, data revealed that all treated groups showed non-significant differences in blood parameters experiment as compared with control group.

​Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs with White Muscle Disease

2021 ◽  
Author(s):  
Kıvanç Irak ◽  
Handan Mert ◽  
Nihat Mert ◽  
Nesrullah Ayşin ◽  
İnci Doğan Söğütlü ◽  
...  
Keyword(s):  
Vitamin E ◽  
Total Protein ◽  
Biochemical Parameters ◽  
White Muscle ◽  
Statistical Significance ◽  
Blood Parameters ◽  
Muscle Disease ◽  
Tissue Samples ◽  
White Muscle Disease ◽  
Blood Samples

Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups.

scholarly journals Effect of restricted-feeding strategies on blood concentrations of hormones, metabolites, and minerals in Holstein × Zebu finishing steers

2020 ◽  
Vol 21 ◽  
Author(s):  
Flávia Adriane de Sales Silva ◽  
Sebastião De Campos Valadares Filho ◽  
Luciana Navajas Rennó ◽  
Nathalia Veloso Trópia ◽  
Caio William Magalhães Souza ◽  
...  
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Experimental Unit ◽  
Feeding Strategies ◽  
Restricted Feeding ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Finishing Steers ◽  
Ad Libitum ◽  
Calcium And Phosphorus

Abstract The objectives were to determine the effects of restricted-feeding strategies on blood concentrations of hormones, metabolites, and minerals in Holstein × Zebu finishing steers. Twenty steers (body weight = 319 ± 16.2 kg; age = 19 ± 1 months) were randomly allotted in 4 groups of 5 steers, which were restricted to 85% ad libitum feed intake for either 0 (R0; n = 5), 28 (R28; n = 5), 42 (R42; n = 5) or 84 (R84; n = 5) days of an 84-d finishing period. Steers were individually housed and considered the experimental unit. Blood samples were collected on d 0, 21, 42, 63, and 84, and were assayed for insulin-like growth factor 1 (IGF-1), thyroid hormones (T3 and T4), albumin, creatinine, urea, total protein, glucose, triglycerides, calcium, and phosphorus. Steers had increasing IGF-1 concentrations until d 42 when restricted-fed. Steers fed R84 had the greatest IGF-1 concentrations on d 84. Urea concentrations were greatest in steers fed R0 and least in steers fed R28; steers fed R42 and R84 were intermediate and not different from one another. Concentrations of IGF-1, urea, albumin, and calcium, but not T3, T4, glucose, triglycerides, creatinine, total protein, and phosphorus were affected by restricted-feeding strategies in Holstein × Zebu finishing steers.

scholarly journals Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 442
Author(s):  
Debora Muratori Holanda ◽  
Young Ihn Kim ◽  
Wanpuech Parnsen ◽  
Sung Woo Kim
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Hepatic Function ◽  
Negative Effects ◽  
Serum Total Protein ◽  
Urea Nitrogen ◽  
Inflammatory Status ◽  
Tnf Α ◽  
Mycotoxin Adsorbent

Phytobiotics with a mycotoxin adsorbent were used to mitigate negative effects of multiple mycotoxins in diets fed to pigs. In experiment 1, 120 pigs (11.6 kg body weight; BW) were assigned to five treatments (three pigs/pen) and fed for 28 days. Treatments were CON (control), MTD (CON + 2.5 mg/kg of deoxynivalenol), DP (MTD + phytobiotics at 0.1%), and DPA1 and DPA2 (MTD + phytobiotics and adsorbent at 0.1% and 0.2%, respectively). In experiment 2, 96 pigs (28.5 kg BW) were assigned to four treatments (three pigs/pen) and fed for 26 days. Treatments were CON, MTAF (CON + 0.19 mg/kg of aflatoxin and 8 mg/kg of fumonisins), AFP (MTAF + phytobiotics at 0.1%), and AFPA (MTAF + phytobiotics and adsorbent at 0.1%). Growth performance was measured weekly, and blood was sampled at the end of study to measure hepatic function and inflammatory status (TNF-α). Data were analyzed using the MIXED procedure. In experiment 1, pigs fed MTD, DP, DPA1, and DPA2 had smaller (p < 0.05) BW than CON. Pigs fed DPA2 had greater (p < 0.05) BW than MTD. Pigs fed DP and DPA2 tended to have lower (p < 0.1) serum total protein than CON. Pigs fed MTD and DPA2 tended to have higher (p < 0.1) alanine aminotransferase than CON. Similarly, pigs fed MTD, DP, and DPA2 tended to have higher (p < 0.1) urea nitrogen/creatinine than CON. In experiment 2, pigs fed MTAF, AFP, and AFPA had smaller (p < 0.05) BW than CON. Pigs fed MTAF, AFP, and AFPA had smaller (p < 0.05) ADFI than CON. Pigs fed AFPA had higher (p < 0.05) aspartate aminotransferase than CON and MTAF. Pigs fed AFP and AFPA had higher (p < 0.05) alanine aminotransferase than CON. Pigs fed MTAF, AFP, and AFPA had lower (p < 0.05) urea nitrogen/creatinine than CON. Pigs fed AFPA had higher (p < 0.05) TNF-α than CON and MTAF. In conclusion, feeding an additional 2.5 mg/kg of deoxynivalenol or 0.19 mg/kg of aflatoxin with 8 mg/kg of fumonisins reduced the growth of pigs. Deoxynivalenol compromised the hepatic function of pigs. Phytobiotics with adsorbent could partly overcome the detrimental effects of mycotoxins.

scholarly journals Biochemical Assessment of Renal and Liver Function among Preeclamptics in Lagos Metropolis

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Oloruntoba Ayodele Ekun ◽  
Oluwatumininu Mary Olawumi ◽  
Christian Chigozie Makwe ◽  
Nkeiruka Ogochukwu Ogidi
Keyword(s):  
Uric Acid ◽  
Liver Function ◽  
Total Protein ◽  
Venous Blood ◽  
Urine Samples ◽  
Plasma Sodium ◽  
Cross Sectional ◽  
Blood Samples ◽  
Spot Urine ◽  
Specific Syndrome

Objectives. Preeclampsia is a pregnancy specific syndrome. Studies have shown that preeclampsia has multiorgan dysfunction effects. This study evaluated biomarkers of renal and liver function among preeclamptic Nigerian women. Study Design. This was a cross-sectional study conducted among 49 preeclamptic women and 50 normotensive healthy pregnant women. Method. The baseline data comprising age, gestational age, and blood pressure were obtained. Venous blood and spot urine samples were collected from each participant. Plasma obtained from blood samples taken into lithium heparinized vacutainer bottles was assayed for electrolytes, urea, creatinine, total protein, albumin, and uric acid, while sera samples from blood samples taken into serum separation tube- (SST-) gel vacutainer were assayed for aspartate transaminase and alanine transaminase using ion selective electrode technique and Cobas autoanalyzer. Spot urine samples were assayed for protein and creatinine using Pyrogallol’s reagent and Jaffe’s methods, respectively. Microalbuminuria (protein/creatinine ratio) was generated from spot urine protein and creatinine data. Result. The plasma sodium, total protein, and albumin in preeclamptic group were significantly decreased (p<0.05) when compared with control. There was statistically significant increase (p<0.05) in microalbuminuria, plasma potassium, urea, creatinine, uric acid levels, serum AST, and ALT activities in preeclamptic group. A positive association (p<0.05) between alanine aminotransferase and biomarkers of renal function was observed. Conclusion. Preeclampsia has deleterious effects on renal and liver function as shown by alteration of these parameters.

PARA-AMINOBENZOIC ACID IN THE TREATMENT OF ROCKY MOUNTAIN SPOTTED FEVER

PEDIATRICS ◽  
1948 ◽  
Vol 2 (2) ◽  
pp. 163-174
Author(s):  
SIDNEY ROSS ◽  
PRESTON A. MCLENDON ◽  
HUGH J. DAVIS
Keyword(s):  
Body Weight ◽  
Early Diagnosis ◽  
Blood Concentration ◽  
Glucuronic Acid ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Optimal Time ◽  
Aminobenzoic Acid ◽  
Rocky Mountain Spotted Fever ◽  
Therapeutic Results

PABA was used in 17 cases of Rocky Mountain spotted fever during the summers of 1946 and 1947 with recovery of all patients. Both the morbidity and degree of toxicity were decreased. The earlier the initiation of PABA therapy, the better the response. Favorable therapeutic results can probably be expected if the drug is started before the seventh day. The optimal time for initiating treatment probably is during the fist four days after the onset of the disease. The importance of early diagnosis is emphasized. There is considerable variation in the PABA blood level achieved with comparable dosage, there being no definite linear or curvilinear correlation between dosage and blood concentration. On the average, a dose of 0.9 gm./kg. body weight in 24 hours in a two-hour divided dosage schedule was found to be satisfactory in children. PABA has few toxic manifestations. A leukopenia without granulocytopenia was found to occur in some cases. Cephalin flocculation tests showed an increase in positivity during PABA therapy. A reducing substance which produced a green or yellow reduction with Benedict's reagent was found in 55% of the urines examined during PABA therapy. This reducing substance is probably a benzoylglucuronide resulting from the conjugation of PABA with glucuronic acid.

PSXII-28 Effects of the nutritional plane before and after breeding on performance of different hair sheep breeds

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 489-490
Author(s):  
Farida Belkasmi ◽  
Raquel V Lourencon ◽  
Ryszard Puchala ◽  
Terry A Gipson ◽  
Luana Ribeiro ◽  
...  
Keyword(s):  
Body Weight ◽  
Birth Weight ◽  
Wheat Straw ◽  
Litter Size ◽  
Birth Rate ◽  
Average Daily Gain ◽  
Breeding Period ◽  
Hair Sheep ◽  
Cereal Grain ◽  
Before And After

Abstract Female hair sheep, 27 Dorper (DOR), 41 Katahdin (KAT), and 39 St. Croix (STC), were used to determine influences of nutritional planes before and after breeding on performance. There were 35 multiparous and 72 primiparous sheep, the latter 2.8±0.20 yr of age. Wheat straw [4% crude protein; dry matter (DM) basis] was consumed ad libitum and supplemented with approximately 0.25% initial body weight (BW) of soybean meal (SBM; Low) or a 1:3 mixture of SBM and rolled corn at 1% BW (High; DM). The supplementation period was 162 d, and with breeding of animals in 2 groups sequentially the pre-breeding period was 84 and 97 d and that after breeding began was 78 and 65 d. Wheat straw DM intake (1.75, 1.30, 1.57, 1.15, 1.80, and 1.38% BW; SEM=0.112), average daily gain (-46, 42, -44, 70, -47, and 51 g; SEM=7.3), and change in body condition score (-0.61, 0.36, -0.53, 0.27, -0.39, and -0.18 for DOR-Low, DOR-High, KAT-Low, KAT-High, STC-Low, and STC-High, respectively; SEM=0.058) were influenced (P &lt; 0.05) by supplement treatment. Birth rate (66.7, 93.5, 84.6, 95.5, 82.8, and 100.0; SEM=9.83) and individual lamb birth weight (4.50, 4.61, 4.28, 3.98, 3.73, and 3.88 kg; SEM=0.201) were not affected by supplement treatment (P = 0.063 and 0.787, respectively), although litter size (0.92, 1.21, 1.17, 1.86, 1.12, and 1.82; SEM=0.221) and total litter birth weight (5.84, 5.74, 5.92, 7.52, 5.04, and 6.78 kg for DOR-Low, DOR-High, KAT-Low, KAT-High, STC-Low, and STC-High, respectively; SEM=0.529) were greater (P &lt; 0.05) for High than for Low. In conclusion, although there was some compensation in wheat straw intake for the different levels of supplementation, SBM given alone rather than with cereal grain adversely affected body weight and condition and reproductive performance, the latter primarily through litter size but also via a trend for an effect on birth rate.

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