Relationship Between Gas Emissions and Dry Matter Intake in Mature Angus Cows

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 32-33
Author(s):  
Amanda Holder ◽  
Megan A Gross ◽  
Alexi Moehlenpah ◽  
Paul Beck

Abstract The objective of this study was to examine the effects of diet quality on greenhouse gas emissions and dry matter intake (DMI). We used 42 mature, gestating Angus cows (600±69 kg; and BSC 5.3±1.1) with a wide range in DMI EPD (-1.36 to 2.29). Cows were randomly assigned to 2 diet sequences forage-concentrate (FC) or concentrate-forage(CF) determined by the diet they consumed in each period (forage or concentrate). The cows were adapted to the diet and the SmartFeed individual intake units for 14 d followed by 45 d of intake data collection for each period. Body weight was recorded on consecutive weigh days at the beginning and end of each period and then once every two wk for the duration of a period. Cows were exposed to the GreenFeed Emission Monitoring (GEM) system for no less than 9 d during each period. The GEM system was used to measure emissions of carbon dioxide (CO2) and methane (CH4). Only cows with a minimum of 20 total >3-m visits to the GEM were included in the data set. Data were analyzed in a crossover design using GLIMMIX in SASv.9.4. Within the CF sequence there was a significant, positive correlation between TMR DMI and CH4 (r=0.81) and TMR DMI and CO2 (r=0.69), however, gas emissions during the second period on the hay diet were not correlated with hay intake. There was a significant, positive correlation between hay DMI and CO2 (r=0.76) and hay DMI and CH4 (r=0.74) when cows first consumed forage (FC). In comparison to the CF sequence, cows on the FC sequence showed a positive correlation between CO2 and TMR DMI during the second period. There was also a significant positive correlation between hay and TMR DMI when assessed across (r=0.43) or within sequence (FC r=0.41, CF r=0.47).

2013 ◽  
Vol 12 (5) ◽  
pp. 452-457 ◽  
Author(s):  
George A. Alexiou ◽  
George Vartholomatos ◽  
Kalliopi Stefanaki ◽  
Amalia Patereli ◽  
Lefkothea Dova ◽  
...  

Object Medulloblastoma (MB) is the most common malignant brain tumor in children. Heat shock proteins (HSPs) comprise a superfamily of proteins that serve as molecular chaperones and are overexpressed in a wide range of human cancers. The purpose of the present study was to investigate the expression of HSP27 (pSer82), HSP27 (pSer15), HSP40, HSP60, HSP70, HSP90-α, Akt, and phospho-Akt by multiplex bead array assay of MBs. The results of HSP and Akt expression were correlated with MB subtype; immunohistochemical expression of Ki-67 index, bcl-2, and p53; and patients' prognosis. Methods The authors retrospectively evaluated 25 children with MB who underwent surgery. Immunohistochemical analysis of Ki-67, p53, and bcl-2 expression was performed in all cases. By using multiplex bead array assay, a simultaneous detection of HSP27 (pSer82), HSP27 (pSer15), HSP40, HSP60, HSP70, HSP90-α, Akt, and phospho-Akt was performed. Results Medulloblastoma with extensive nodularity had significantly lower HSP27 (pSer15) expression (p = 0.039) but significantly higher HSP60 expression (p = 0.021) than classic MB. Large-cell MB had significantly higher HSP70 expression (p = 0.028) than classic MB. No significant difference was found between HSP27 (pSer82), HSP40, HSP90-α, Akt, or phospho-Akt expression and MB subtype. Large-cell MBs had significantly higher Ki-67 index compared with classic MBs (p = 0.033). When analyzing all MBs, there was a significant negative correlation between HSP27 (pSer15) and Ki-67 index (r = −0.475, p = 0.016); a significant positive correlation between HSP70 expression and Ki-67 index (r = 0.407, p = 0.043); and a significant positive correlation between HSP70 expression and bcl-2 index (r = 0.491, p = 0.023). Patients with large-cell MB had a worse survival than those with classic MB, but the difference did not reach statistical significance (p = 0.076). Conclusions A substantial expression of several HSPs in MB was observed. Given that HSPs represent an attractive strategy for anticancer therapy, further studies, involving larger series of patients, are obviously necessary to clarify the relationship of HSPs with tumor aggressiveness and prognosis.


2008 ◽  
Vol 88 (4) ◽  
pp. 693-701 ◽  
Author(s):  
M. Undi ◽  
C. Wilson ◽  
K. H. Ominski ◽  
K. M. Wittenberg

Four techniques were compared for their ability to estimate forage dry matter intake (DMI) of grazing animals. In the Cage technique, 10 grazing cages were placed within each pasture, and forage inside and outside the cages was clipped from 0.25-m2 quadrats after 12 to 20 d of grazing. The difference between forage inside and outside the grazing cage represented forage consumed by grazing animals. The second technique used n-alkane controlled release capsules to measure DMI of individual grazing animals (N-alkane marker technique). The remaining two techniques were prediction equations; one utilized body weight (BW) and average daily gain (ADG) to estimate DMI (Minson equation) and the other related dietary net energy concentration and BW to DMI (Net Energy equation). The four methodologies were applied to a 3-yr study evaluating liquid hog manure on the productivity of grass pastures. The study utilized steers of a relatively uniform body size on six continuously grazed grass pastures. When individual animal intakes were compared, the Net Energy equation estimated lower (P < 0.05) DMI than the Minson equation, with the N-alkane marker technique being intermediate. Dry matter intake (mean ± SD, kg d-1) was 5.3 ± 0.76, 7.4 ± 1.86, and 6.5 ± 2.58 for the Net Energy equation, Minson equation, and N-alkane marker technique, respectively. The Cage technique estimated average DMI of animals in each pasture of 17.5 ± 11.61 kg d-1 and this was higher (P < 0.05) than estimates from the other techniques. Dry matter intake estimates from different techniques were ranked: Cage technique > Minson equation > N-alkane marker technique > Net Energy equation. The DMI estimates generated by the Cage technique were associated with the largest coefficient of variation, with extreme values representing a range from 0.3 to 15.2% BW. The N-alkane marker technique estimated DMI that ranged from 0.6 to 4.5% BW. The strongest correlation (r = 0.30; P = 0.001) in DMI estimates was between the Net Energy and Minson equations due to the inclusion of BW as a component in both equations. Results of this study suggest that prediction equations can be useful for rapid DMI estimation. The N-alkane marker technique may be the preferred technique when a wide range of pasture conditions is anticipated. Key words: Grazing cages, n-alkanes, prediction equations, dry matter intake, grazing animals


2017 ◽  
Vol 17 (14) ◽  
pp. 9019-9033 ◽  
Author(s):  
Thomas G. Bell ◽  
Sebastian Landwehr ◽  
Scott D. Miller ◽  
Warren J. de Bruyn ◽  
Adrian H. Callaghan ◽  
...  

Abstract. Simultaneous air–sea fluxes and concentration differences of dimethylsulfide (DMS) and carbon dioxide (CO2) were measured during a summertime North Atlantic cruise in 2011. This data set reveals significant differences between the gas transfer velocities of these two gases (Δkw) over a range of wind speeds up to 21 m s−1. These differences occur at and above the approximate wind speed threshold when waves begin breaking. Whitecap fraction (a proxy for bubbles) was also measured and has a positive relationship with Δkw, consistent with enhanced bubble-mediated transfer of the less soluble CO2 relative to that of the more soluble DMS. However, the correlation of Δkw with whitecap fraction is no stronger than with wind speed. Models used to estimate bubble-mediated transfer from in situ whitecap fraction underpredict the observations, particularly at intermediate wind speeds. Examining the differences between gas transfer velocities of gases with different solubilities is a useful way to detect the impact of bubble-mediated exchange. More simultaneous gas transfer measurements of different solubility gases across a wide range of oceanic conditions are needed to understand the factors controlling the magnitude and scaling of bubble-mediated gas exchange.


Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1006 ◽  
Author(s):  
S. Richard O. Williams ◽  
Murray. C. Hannah ◽  
Joe L. Jacobs ◽  
William J. Wales ◽  
Peter J. Moate

The dry matter intake (DMI) of forage-fed cattle can be used to predict their methane emissions. However, many cattle are fed concentrate-rich diets that decrease their methane yield. A range of equations predicting methane yield exist, but most use information that is generally unavailable when animals are fed in groups or grazing. The aim of this research was to develop equations based on proportions of ruminal volatile-fatty-acids to predict methane yield of dairy cows fed forage-dominant as well as concentrate-rich diets. Data were collated from seven experiments with a total of 24 treatments, from 215 cows. Forage in the diets ranged from 440 to 1000 g/kg. Methane was measured either by open-circuit respiration chambers or a sulfur hexafluoride (SF6) technique. In all experiments, ruminal fluid was collected via the mouth approximately four hours after the start of feeding. Seven prediction equations were tested. Methane yield (MY) was equally best predicted by the following equations: MY = 4.08 × (acetate/propionate) + 7.05; MY = 3.28 × (acetate + butyrate)/propionate + 7.6; MY = 316/propionate + 4.4. These equations were validated against independent published data from both dairy and beef cattle consuming a wide range of diets. A concordance of 0.62 suggests these equations may be applicable for predicting methane yield from all cattle and not just dairy cows, with root mean-square error of prediction of 3.0 g CH4/kg dry matter intake.


1978 ◽  
Vol 58 (2) ◽  
pp. 177-181 ◽  
Author(s):  
U. I. OJI ◽  
D. N. MOWAT

Corn stover, ground through a 1.6-cm screen, was steam-processed at 16.2 kg/cm2 and 205 °C for 15 min. Control and treated stover were evaluated for intake and digestibility of organic constituents with eight wether lambs in a single crossover design. Prior to feeding, water was added to control corn stover to approximate the moisture content of treated stover and to reduce dustiness. Soybean meal was added to both rations and, in addition, urea was added to treated stover to attempt to make up for nitrogen rendered unavailable by treatment. Steam treatment increased (P < 0.05) ADF, ADIN, permanganate lignin and acetic acid. Dry matter intake increased (P < 0.05) by 55% with steam treatment. Treatment also increased (P < 0.05) apparent digestibilities of organic matter, energy, non cell wall contents and cellulose but decreased (P < 0.05) digestibilities of NDF and ADF. The extent of improvement in energy digestibility was limited by heat damage due to processing. Modifications in treatment conditions should reduce this effect.


2015 ◽  
Vol 77 ◽  
pp. 177-184 ◽  
Author(s):  
D.F. Chapman ◽  
P.D. Muir ◽  
M.J. Faville

Persistence of herbage yield is an important productivity trait of grass species and cultivars in New Zealand pastures. However, relatively little is known about genetic variation in this trait, principally because few studies comparing yield have continued beyond 3 years. This paper reports results from a comparison of 25 perennial ryegrass cultivars representing a wide range of functional types and genetic backgrounds conducted under sheep grazing in a summer-dry environment. Dry matter (DM) yield was measured for the first 3 years after sowing, then again in years seven and eight post-sowing, and in year ten post-sowing. Endophyte DNA fingerprinting conducted mid-way through year seven confirmed that, with one exception, ryegrass populations remained true-to-type in the cultivars sown with novel endophyte strains. The cultivar effect on DM yield was statistically significant in all measurement periods. Differences in yields among cultivars in years seven and eight were significantly and positively correlated with yield differences in years one to three (r=0.685 to 0.831 depending on which year contrasts were used). Thus, high-performing cultivars in the early years of the trial were also generally highperforming cultivars in years seven and eight, and vice-versa, indicating a high degree of yield stability in perennial ryegrass cultivars. Yield differences did not appear to be related to differences in cultivar heading date, ploidy, endophyte status, or genetic background. The relationship between yield in year ten and yields in years one to three was much weaker (r=0.392) than the relationship in years seven and eight. Possible reasons for this are discussed. Keywords: Perennial ryegrass; traits; persistence


2020 ◽  
Vol 60 (6) ◽  
pp. 780
Author(s):  
Vinu M. Nampoothiri ◽  
Madhu Mohini ◽  
Bilal Ahmad Malla ◽  
Goutam Mondal ◽  
Sujata Pandita

The present study aimed to evaluate the effects of dietary forage:concentrate ratios on growth performance and enteric and faecal greenhouse-gas emissions from growing buffalo calves. Fifteen Murrah male calves (bodyweight = 233.35 ± 30.92 kg; 8–12 months age) were randomly assigned to three dietary groups that were fed a mixture of berseem fodder, wheat straw and concentrate at the ratios of 20:60:20 (C20), 20:40:40 (C40) and 20:20:60 (C60) respectively, for 120 days. Enteric methane (CH4) production was estimated by the sulfur hexafluoride tracer technique. Faeces were stored for 12 weeks and CH4 and nitrous oxide (N2O) fluxes from stored faeces were estimated every 14 days. Dry-matter intake, feed conversion efficiency and nitrogen retention were not affected (P &gt; 0.05) but average daily gain and urinary nitrogen loss (g/day) were higher for C60 than the C20 diet (P &lt; 0.05). Daily enteric CH4 emission (g/day) was not affected but CH4 yield (g/kg dry-matter intake) and energy loss through CH4 as a proportion of energy intake were lower for C60 than the C20 diet (P &lt; 0.05). Faeces composition was not affected, and large variations of greenhouse-gas emissions were observed for first 10 days of storage. Methane emissions from stored faces were 1.28 ± 0.40, 1.94 ± 0.34 and 3.90 ± 0.27 mg/kg faeces per day for C20, C40 and C60 diets respectively, being higher for C60 than the C40 and C20 diets (P &lt; 0.05). Methane-flux rate from faeces was greater for C60 than the C20 and C40 diets (0.75 vs 0.26 and 0.37 g/animal respectively; P &lt; 0.05). Diet C60 increased N2O fluxes from stored faeces by 63% and 58% respectively, expressed in mg/kg faeces per day and mg/animal per day, compared with C20 diet (P &lt; 0.05). Overall, dietary concentrate proportion of up to 60% in growing buffalo calf diets improved growth performance without increasing enteric CH4 emission, but CH4 and N2O production from faeces were increased. This work has provided information for gas emissions factors from open storage of faeces. More detailed studies on gaseous emissions from open lots on farms are required.


1978 ◽  
Vol 18 (92) ◽  
pp. 391 ◽  
Author(s):  
RA Hunter ◽  
CP: Siebert BD Miller

Merino wethers held in individual metabolism crates were fed one of the following: a control diet of Stylosanthes guianensis; S. guianensis supplemented with 0.5 g sulphate-sulphur per head per day; or S. guianensis which had been fertilized with superphosphate (1 2 per cent S). The daily dry matter (DM) intakes and DM digestibilities of the three diets were 142 g, 0.203; 202 g, 0.296; 268 g, 0.352 respectively. The digestible dry matter intake of the animals supplemented with sulphate-sulphur was, therefore, double that of the controls, and that of animals fed fertilized hay, treble. The magnitude of the responses in each case were modified by the availability of other dietary constituents, notably nitrogen. In a second period DM intake of sheep supplemented with sulphate-sulphur and those fed fertilized S. guianensis was further increased by supplements of sodium chloride.


Author(s):  
Vita Šterna ◽  
Sanita Zute ◽  
Zaiga Vicupe

Abstract Consumption of dietary fibre has potential in improving nutrition and health. For further breeding of oats, it is necessary to increase knowledge on variation in the content of fibre and bioactive components in oats. Therefore, the aim of the present investigation was to study the extent of variation in β-glucan concentration of oats grown in Latvia and to evaluate association of β-glucan concentration with protein and fat concentration in newly created oat breeding lines. In 2015 and 2016, concentration of protein, fat, and β-glucan in grain of hulled oat varieties ‘Laima’, ‘Peppi’, and ten breeding lines was determined. The obtained results showed a wide range of fat concentration among varieties, with a range from 5.9 to 8.3%. The concentration of β-glucan ranged from 1.8 to 3.6% depending on the year. The β-glucan concentration had medium close positive correlation with lipid and weak significant positive correlation with protein concentration, which significantly differed among genotypes.


2019 ◽  
Vol 11 (24) ◽  
pp. 7129
Author(s):  
Alessia Spada ◽  
Mariantonietta Fiore ◽  
Umberto Monarca ◽  
Nicola Faccilongo

The achievement of the objectives of reducing greenhouse gas (GHG) emissions has increasingly received attention and support from decision makers and research by scholars. The livestock sector has always been one of the major sources of GHG emissions, especially in developing countries that do not have green technologies to improve the management of livestock waste. In order to achieve an absolute reduction in emissions, developed countries have applied a wide range of mitigation options; however, there are few studies from the developing world, although greenhouse gas emissions in developing countries have registered a rapid growth. Therefore, this research aims to assess and understand whether public R&D investments can affect emissions deriving from the livestock sector in developing countries. We made use of the FAOSTAT data (FAO Statistical Databases United Nations) and ASTI data set (Agricultural Science and Technology Indicators), collecting data from 29 Africa countries, in 2014 (latest data available). The data were analyzed by means of a Generalized Propensity Scores (GPS) approach, an increasingly widespread technique that is more robust than regression models, especially in small datasets. Our analysis suggests that the livestock sector in these countries shows an improvement in its relationships with the environment and GHG emission levels when the level of public R&D (Research and Development) investment on agriculture is greater. Therefore, reducing greenhouse gas emissions by investing in research and development can lead to more efficient and sustainable resource management for developing countries.


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