Development of the original whey‐based vinegar using rapeseed meal or wheat bran as a raw material for koji

Author(s):  
Yusuke Kawamata ◽  
Yosuke Toyotake ◽  
Daiki Ogiyama ◽  
Yoichi Takeda ◽  
Mamoru Wakayama
2011 ◽  
Vol 183-185 ◽  
pp. 411-416
Author(s):  
Xi Qin Wang ◽  
Zheng Wen Huang ◽  
Bo Yu ◽  
Jian Qiang Zhang

As an industrial organic solid waste, oil residue, with wide sources and low price, but could not find an effective way to use currently. In addition, with the development of mushroom industry and rising price of traditional raw-materials, it is very necessary to find the substitute raw material to reduce production cost of mushroom cultivation. This study attempts to produce the strains of Flammulina velutipes by taking use of oil residue to replace part of the traditional raw material, there are two groups in the experiment: the test group is adding 5%, 10%, 15%, 20%, 40% of the oil residue to replace the culture medium of cottonseed hull and wheat bran; the control group is the traditional culture medium, then comparatively observed the growth of the mycelium. The results showed that it can promote the growth of the mycelium and shorten the cycle of seed production to add the oil residue in the test. But the growth rate of mycelium will be hindered when the concentration exceeds a certain range.The optimal culture medium to add the oil residue is as follows: 15% of the oil residue, 64% cottonseed hull, 20% coarse wood chips, 0% wheat bran , 1% lime. Oil residue contains a lot of the nutritional components to benefit the growth of edible fungi, and without the heavy metal pollution, can replace the culture medium of cottonseed hull and wheat bran and other traditional materials, reduce cost of production the strains, a fair-sized economic efficiency can be received, the environment can be protected.


2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ines Ayadi ◽  
Hafedh Belghith ◽  
Ali Gargouri ◽  
Mohamed Guerfali

The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis. On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content. This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose. Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid. An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes. The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material. Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements. Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively. The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran. The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.


2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenchao Chen ◽  
Xuan Li ◽  
Xuli Ma ◽  
Shouwen Chen ◽  
Yanping Kang ◽  
...  

Abstract Background Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Especially, the effect of oil in RSC on iturin A production and the possibility of using lipases to improve the iturin A production were analyzed in batch fermentation. Results The maximum production of iturin A was 0.82 g/L at the optimal initial RSC and glucose concentrations of 90 and 60 g/L, respectively. When RSC was substituted with RSM as nitrogen source based on equal protein content, the final concentration of iturin A was improved to 0.95 g/L. The production of iturin A was further increased by the addition of different lipase concentrations from 0.1 to 5 U/mL into the RSC medium for simultaneous hydrolysis and fermentation. At the optimal lipase concentration of 0.5 U/mL, the maximal production of iturin A reached 1.14 g/L, which was 38.15% higher than that without any lipase supplement. Although rapeseed oil and lipase were firstly shown to have negative effects on iturin A production, and the effect would be greater if the concentration of either was increased, their respective negative effects were reduced when used together. Conclusions Appropriate relative concentrations of lipase and rapeseed oil were demonstrated to support optimal iturin A production. And simultaneous hydrolysis with lipase and fermentation was an effective way to produce iturin A from RSC using B. amyloliquefaciens CX-20.


Author(s):  
Tetiana V. Kulik ◽  
◽  
Nataliia S. Nastasiienko ◽  
Borys B. Palianytsia ◽  
Kostiantyn S. Kylik ◽  
...  

Lignocellulose is currently considered as a potential renewable source of a wide range of valuable chemicals, including aromatics. Catalytic pyrolysis is the promising method for the conversion of biomass raw materials. The development of renewable biomass pyrolysis technologies requires fundamental research on catalytic thermal transformations of lignocellulosic raw materials. Therefore, in this work, the methods of IR spectroscopy, temperature programmed desorption mass spectrometry (TPD MS) and thermogravimetric analysis were used to investigate the catalytic thermal transformations of rapeseed meal (RM) and ferulic acid (FA) as a model phenol-containing component of such raw material on the surface of nanosized oxides CeO2, SiO2 TiO2/SiO2, Al2O3/SiO2 and CeO2/SiO2. The most effective catalyst in the conversion of biomass to ketones was a nanocomposite CeO2/SiO2 with the highest content of nanoparticles of CeO2 (24%). According to the data of FTIR spectroscopic studies, the interaction of FA with the CeO2 surface occurs with the participation of phenol and carboxyl groups. The main products of thermal decomposition of FA on the surface of CeO2 are 3-methoxy-4-vinylphenol, guaiacol, coumaric acid and hydroxybenzene. Condensed aromatics (naphthalene, alkylnaphthalenes) were registered in small quantities.


2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Ravindra H. Patil ◽  
Prakash Krishnan ◽  
Vijay L. Maheshwari

A wild fungal strain of Aspergillus terreus, labeled as PM3, was isolated by using the Candida albicans bioassay and confirmed by 18S r DNA analyses. Lovastatin was produced by submerged and solid state fermentations. Of the 30 isolated fungal strains, 11 showed lovastatin production with Aspergillus terreus PM3 being the best with a yield of 240 mg/L at the 10th day of submerged fermentation. Carboxymethylcellulose had a stimulatory effect on lovastatin production. It restricted uncontrolled filamentous growth, induced pellet formation and, thereby, improved lovastatin yield. In solid state fermentation (SSF), of the agro wastes from five crops (bran of wheat and rice, husks of red gram and soybean, and green gram straw), wheat bran showed maximum lovastatin production (12.5 mg/g of dry substrate) at pH 7.1 and a temperature of 30±2°C. Development of a lovastatin production process based on wheat bran as a substrate in SSF is economically attractive as it is a cheap and readily available raw material in agriculture-based countries.


2020 ◽  
Vol 14 (1) ◽  
Author(s):  
M. Buzhylov ◽  
L. Kaprelyants ◽  
L. Pozhitkova ◽  
A. Kishenya ◽  
A. Soboleva

The article presents conceptual approaches to solving technological and technical problems in the creation of functional foods. General approaches are proposed to change existing technologies to improve the efficiency of integrated raw material processing and to increase the production of high-quality foods and food ingredients with antioxidant properties. Cereal crops are the richest source of functional ingredients and a major component of human nutrition. It is proved that most of the nutrients are in the products of its processing. For the first time, polyphenols from cereal raw materials were obtained by biotechnological means. The feasibility of pretreatment of raw materials with amylolytic and proteolytic enzymes for purification and cleavage of polysaccharide matrix has been established. Based on the regularities of enzymatic hydrolysis of polysaccharides, we used the processing of wheat bran with multifunctional drug Viscozyme L with hemicellulase, cellulase, pectinesterase and feruloesterase activities, which resulted in a high effect of degradation of certain covalent cells, ferulic acid from 40.99 to 2507.9 mcg / g. It is determined that this method of obtaining the target components allows to preserve their native structure, especially the supramolecular structure, which determines their physiological effect. The influence of plant polyphenols on the cultivation of probiotic microorganisms is characterized. the comparative characterization of the prebiotic properties of the polyphenols obtained from wheat bran and the concentrate of the polyphenols from the grape buds "ENOANT" are substantiated. The possibility of increasing the proportion of free polyphenols by fermentation of wheat bran is shown. It is established that the extract of polyphenols from wheat bran can be used for its purpose as an effective antioxidant, which does not have a negative effect on the state of the basic physiological systems of the body.


Author(s):  
E. E. Kurchaeva ◽  
T. N. Tertychnaya ◽  
A. A. Shevtsov ◽  
N. A. Serdyukova ◽  
N. Y. Sitnikov

The organization of scientifically justified feeding of farm animals with compound feed enriched with oilseed processed products as an energy component and a probiotic component contributing to the regulation of homeostasis processes becomes important in modern conditions of animal husbandry production. At the same time, a special role is given to the application of rapeseed products, meals in particular, in feed rations. Rapeseed meal contains protein, carbohydrates, fats, phospho-containing substances in a concentrated form, which expands the possibilities of the raw material base for the development of compound feed recipes that meet the requirements of complete nutrition for animals. The dosage of rapeseed meal (10%) introduction into the composition of compound feed for young rabbits based on the animals receiving nutrients in accordance with the existing feeding standards was substantiated in the work. Evaluation of the effectiveness of the obtained granulated feed was carried out on a clinically healthy livestock of rabbits in the amount of 60 heads, which were divided into 4 groups. The best indicators of meat productivity (slaughter yield 69.80%, meat index 4.40) were obtained with the introduction of the probiotic preparation "Probion - Forte" at a dosage of 0.5 g / kg of feed and rapeseed meal in an amount of 10% to the feed mass. The application of the developed complete granulated feed made it possible to achieve a high degree of the diet protein component concentration in the muscle tissue of experimental rabbits, which indicates a higher nutritional and biological value of the meat resources obtained.


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