scholarly journals Characterizing Selected Sorghum Grain Varieties and Evaluating the Suitability of Their Malt Extracts for Cultivating Microbial Biomass

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Stellah Byakika ◽  
Ivan Muzira Mukisa ◽  
Yusuf Byenkya Byaruhanga
Keyword(s):  
Microbial Biomass ◽  
Food Processing ◽  
Crude Protein ◽  
Amino Nitrogen ◽  
Growth Media ◽  
Total Sugars ◽  
Kernel Length ◽  
Significant Difference ◽  
Sorghum Malt ◽  
Sorghum Grain

Microbial biomass is cultivated for different technological applications including food processing, medicine, waste management, and research. The conventional growth media used are generally expensive thus necessitating the development of more affordable alternatives. In this study, four sorghum grain varieties, SESO 1, SESO 3, Epuripur, and Eyera, and their malt extracts were characterized which is aimed at determining their suitability for growing microbial biomass. The varieties had kernel length, kernel width, kernel thickness, and thousand kernel weigh equivalent to 3.8-4.3 mm, 3.2-4.5 mm, 2.4-2.8 mm, and 12.4-20.2 g, respectively. SESO 1 and Epuripur had corneous endosperm textures whereas those of SESO 3 and Eyera were intermediate and floury, respectively. Varieties had germinative   energies > 90 % and total   defects < 8 %. SESO 3 had the highest ( p < 0.05 ) crude protein ( 10.8 ± 0.3 %) and dietary fiber ( 22.5 ± 0.4 %) whereas Epuripur had the highest ( p < 0.05 ) starch ( 81.6 ± 0.0 %) and crude fat ( 2.9 ± 0.1 %). There was no significant difference ( p > 0.05 ) in the ash contents ( 2.1 ± 0.0 %). The total sugars, free amino nitrogen, condensed tannins, and pH of the malt extracts were 106-116 g/L, 70-78 mg/L, 0.1-0.6 mg/mL, and 5.5-5.7, respectively. The composition of the sorghum malt extracts suggests their potential for use in cultivating microbial biomass.

scholarly journals Sorghum Malt Extract as a Growth Medium for Lactic Acid Bacteria Cultures: A Case of Lactobacillus plantarum MNC 21

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Stellah Byakika ◽  
Ivan Muzira Mukisa ◽  
Yusuf Byenkya Byaruhanga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Low Cost ◽  
Growth Media ◽  
Total Sugars ◽  
Microbial Counts ◽  
Cost Growth ◽  
Sorghum Malt ◽  
Mrs Broth

Cultivation of lactic acid bacteria cultures is vital for research and commercial production of fermented foods. However, the conventional growth media used are generally costly. Malt extracts from four sorghum varieties (SESO 1, SESO 3, Epuripur, and Eyera) were evaluated as alternative low-cost growth media for Lactobacillus plantarum MNC 21. Saccharified sorghum malt extracts were inoculated with 4 log cfu/mL MNC 21 and incubated at 30°C for 24 h. MRS broth was the reference medium. Microbial counts, pH, titratable acidity (TA), free amino nitrogen (FAN), and total sugars were measured. Maximum microbial counts in the extracts and MRS broth were 9 and 10 log cfu/mL, respectively. Maximum growth rate in the extracts was 0.7–0.9 log cfu/mL/h and 0.8 log cfu/mL/h in MRS broth. The final pH of the extracts was 3.5–3.6, with an overall increase in TA of 1.2% in Epuripur and 0.2% in other varieties. Final pH and TA of MRS broth were 4.1 and 1.3%, respectively. Total sugars dropped by 95.2% and FAN by 2.1% in MRS broth. In contrast, total sugars and FAN dropped by 5.6–9.1% and 24.9–32.7% respectively, in the extracts. Sorghum malt extracts can be adopted as alternative low-cost growth media for lactic acid bacteria cultures.

CONCENTRADO PROTEICO OBTIDO DAS FOLHAS DE MANDIOCA (Manihot esculenta Crantz) DE TRÊS VARIEDADES COMERCIAIS.

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Izabel Aparecida Soares ◽  
Mauro Sérgio Téo ◽  
Carlise DEBASTIANI ◽  
Suzymeire BARONI ◽  
Vanessa Silva RETUCI
Keyword(s):  
Manihot Esculenta ◽  
Crude Protein ◽  
Hydrocyanic Acid ◽  
Protein Concentrate ◽  
Manihot Esculenta Crantz ◽  
White Variety ◽  
Mean Values ◽  
Randomized Design ◽  
Significant Difference ◽  
The Mean

O trabalho teve por objetivo verificar diferenças entre rendimento do concentrado proteico e proteína bruta da folha da mandioca (Manihot esculenta Crantz), obtidos a partir de três variedades comerciais: branca, cascuda e vermelha. As manivas foram plantadas seguindo o delineamento experimental inteiramente casualizado com três repetições. Nas comparações entre as variedades, considerou coletas escalonadas pós-plantio, realizadas aos 12, 14 e 16 meses. O concentrado proteico foi obtido a partir da farinha das folhas inteiras e submetido ao método de termo - coagulação ácido e a proteína bruta pelo método padrão AOAC. Os dados foram submetidos a análise de variância e comparados pelo teste de Tukey - 5% de probabilidade. Os resultados não indicaram diferença significativa entre as médias obtidas para rendimento de concentrado proteico. Para a variável porcentagem de proteína bruta a variedade Branca foi a que apresentou maior valor, com média de 46,25%, seguida pela Cascuda e Vermelha, 44,52% e 37,30%, sucessivamente. Conclui-se que outros estudos devem avaliar condições que possam influenciar no teor de proteína foliar, como clima e solo, e, avaliar os níveis de ácido cianídrico de cada variedade, indicando qual das três é a melhor para a extração do concentrado proteico das folhas. Palavras chaves: Manihot esculenta Crantz, variedades comerciais, concentrado proteico, proteína bruta. ABSTRACT: The study aimed to assess the differences between income protein concentrate, crude protein of cassava leaf (Manihot esculenta Crantz), obtained from three commercial varieties: white, red and cascuda. The cuttings were planted following the completely randomized design with three replications. Comparisons between the varieties considered after planting staggered collections, held on 12, 14 and 16 months. The protein concentrate was obtained from flour of whole sheets and subjected to the term method - acid coagulation and crude protein by AOAC standard method. The data were submitted to ANOVA and Tukey test - 5% probability. The results indicated no significant difference between the mean values obtained for protein concentrate income. For the variable percentage of crude protein White variety showed the highest, with an average of 46.25%, followed by cascuda and Red, 44.52% and 37.30%, successively. We conclude that further studies should evaluate conditions that may affect the leaf protein content, such as climate and soil, and evaluate the hydrocyanic acid levels of each variety, indicating which of the three is the best for the extraction of protein concentrate from leaves Key words: Manihot esculenta Crantz, commercial varieties, protein concentrate, crude protein.

scholarly journals Seasonal and Temperature Dependent Variations in Proximate Parameters and Mineral Elements of Smoke-Dried Catfish (Clarias gariepinus, Burchell, 1822) using Traditional and Eco-Friendly Kilns in Lagos, Nigeria

2020 ◽  
Vol 24 (4) ◽  
pp. 639-643
Author(s):  
M.M. Ogunbambo
Keyword(s):  
Crude Protein ◽  
Clarias Gariepinus ◽  
Mineral Elements ◽  
Drying Process ◽  
Temperature Dependent ◽  
Crude Fibre ◽  
Significant Difference ◽  
Dry Seasons ◽  
Mineral Compositions ◽  
Wet And Dry Seasons

Smoke-drying Clarias gariepinus (catfish) provides animal protein and a source of livelihood in Lagos, Nigeria. Changes occurring to seasonal and mineral compositions of smoke-dried catfish using local Traditional Drum Kiln (TDK) and a newly constructed Eco-Friendly Kiln (EFK) fitted with a flame, drying and electronic components was carried out in this study. The smoke-drying process was carried out in both wet and dry seasons and smoke-dried catfish samples stored at ambient and adjusted refrigerated temperatures of 28 and 4 0C. The smoke-drying procedure was  standardized at 60 - 80 0C and kiln lasted 24 ± 3 hours. Moisture content results showed a significant difference when the catfish samples were smoke-dried using TDK and EFK and stored in ambient and controlled temperatures in both wet and dry seasons while crude protein, lipid, ash and crude fibre values were significantly different when stored only in dry season. Mineral elements phosphorus, sodium, copper, magnesium and iron showed a significant difference when stored at both temperatures and seasons using both kilns. Mineral elements were found to be most stable in smoke-dried catfish samples when stored at controlled temperatures. This work proved that standardizing smoke-drying process using both kilns resulted in good quality smoke-dried catfish but showed that higher biochemical values were obtained when EFK is used. Key words: Nigeria, Smoke-drying Kilns, Seasons, Ambient, Controlled Temperatures

scholarly journals An Assessment of Treated Greywater Reuse in Irrigation on Growth and Protein Content of Prosopis and Albizia

Horticulturae ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 38
Author(s):  
Naji K. Al-Mefleh ◽  
Yahia A. Othman ◽  
Maher J. Tadros ◽  
Amani Al-Assaf ◽  
Samer Talozi
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Dry Weight ◽  
World Health ◽  
Quality Analysis ◽  
Distilled Water ◽  
Albizia Lebbeck ◽  
Forage Production ◽  
Water Control ◽  
Significant Difference

This study investigated the influence of treated greywater on growth and protein content of multipurpose (forage and ornamental) transplants, Prosopis juliflora L., Prosopis tamarugo L., and Albizia lebbeck L. Transplants of tested species were irrigated with treated greywater, diluted greywater (grey + distilled water, 1:1/by volume), and distilled water (control) for seven months. Water quality analysis showed that the concentrations of nutrients and heavy metals found in the greywater were within the acceptable range compared with Jordan Institution for Standard and Metrology (JISM) and the World Health Organization (WHO) thresholds for safe use of greywater. Escherichia coli found in the greywater were lower compared to JISM and WHO guidelines for the safe use of greywater. Irrigation with treated greywater increased shoot fresh weight by 24–39% and dry weight by 34–40% compared to diluted greywater and control. No significant difference in crude protein was noticed between water treatments. Prosopis species (P. juliflora Albizia lebbeck L. and P. tamarugo Albizia lebbeck L.) had higher shoot fresh (35%) and dry weight the same species had lower crude protein (44%) when compared to Albizia lebbeck Albizia lebbeck L. The reuse of treated greywater for landscaping or forage production alleviates the demand for water resources and reduces the pressure on wastewater treatment plants. However, considering the controversial findings of previous studies on greywater quality (especially, long-term reuse), the reuse of treated greywater needs to be considered with caution and periodic quality analyses and economic assessments are required.

scholarly journals Comparison of agronomic characters of Festulolium, Festuca pratensis Huds. and Lolium multiflorum Lam. genotypes under high elevation conditions in Turkey

1970 ◽  
Vol 37 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Ilknur Akgun ◽  
Metin Tosun ◽  
Suleyman Sengul
Keyword(s):  
Seed Yield ◽  
Crude Protein ◽  
Dry Matter ◽  
Lolium Multiflorum ◽  
High Elevation ◽  
Ash Content ◽  
Festuca Pratensis ◽  
Higher Plant ◽  
Green Matter ◽  
Significant Difference

Prior {Lolium perenne × Festuca pratensis) and Elmet (Lolium multiflorum × Festuca pratensis) cultivars were compared with Festuca pratensis Huds. and Lolium multiflorum Lam. genotypes for some agronomical characteristics (green and dry matter yields, crude protein, seed yield, crude ash content) under Erzurum ecological conditions. Analysis of variance and mean separation were performed using the SPSS 11.0 computer program. There was a significant difference among genotypes in every character tested except for green and dry matter yield and crude ash content. Hybrid genotypes had higher plant height, dry and green matter yield, seed yield per plant and crude protein yield except 1000-seed weight. Elmet performed better than Prior except for seed yield per plant.   Key words: Festulolium, Lolium, Festuca, Yield, Crude protein, Crude ash doi:10.3329/bjb.v37i1.1556 Bangladesh J. Bot. 37(1): 1-6, 2008 (June)

scholarly journals PENGARUH MULTI NUTRIENT SAUCE (MNS) DENGAN DOSIS YANG BERBEDA DALAM RANSUM TERHADAP KECERNAAN PROTEIN KASAR DAN SERAT KASAR PADA DOMBA

2020 ◽  
Vol 4 (1) ◽  
pp. 14-20
Author(s):  
M Alvin Rifqi Fadilla, Erwanto, Muhtarudin, dan Agung Kusuma Wijaya
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Animal Husbandry ◽  
Protein Digestibility ◽  
Crude Fiber ◽  
Significance Level ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Department Faculty ◽  
Difference Test

The purpose of this research was to determine the effect of addition of Multinutrient Sauce (MNS) in the ration on digestibility of crude protein and crude fiber in sheep.  This research was conducted in May—July 2019 at the People's Farm of Kebagusan Village, Gedong Tataan District, Pesawaran Regency.  The digestibility analysis of crude protein and crude fiber was carried out at the Laboratory of Animal Nutrition and Feed, Animal Husbandry Department, Faculty of Agriculture, University of Lampung.  This study used a randomized block design (RBD) based on sheep body weight with six groups and three treatments, namely R0 (ration without MNS); R1 (R0 + MNS 5%); and R2 (R0 + MNS 10%).  The data obtained were analyzed using ANOVA at a 5% significance level or 1% significance, and continued with further test using Least Significant Difference Test (LSD) if there is significant results.  The results showed that the addition of 10% MNS in the ration had a significant effect (P<0.05) on increasing the digestibility of crude protein and crude fiber in sheep.   Keywords: multi nutrient sauce (MNS), digestibility of crude protein, digestibility of crude fiber, sheep.

scholarly journals EFFECT OF VARIOUS DIETARY LEVELS OF SUNFLOWER SEED MEAL ON THE PERFORMANCE OF GROWING RABBITS IN SUDANO-SAHELIAN ZONE

2021 ◽  
Vol 22 (2) ◽  
pp. 150-154
Author(s):  
A. C. Okonkwo ◽  
J. U. Igwebuike ◽  
H. U. Tarfa ◽  
B. I. Okon ◽  
B. I. Umoh
Keyword(s):  
Deleterious Effect ◽  
Sunflower Seed ◽  
Crude Protein ◽  
Seed Meal ◽  
Small Scale ◽  
Considerable Decrease ◽  
Feed Cost ◽  
Significant Difference ◽  
Ad Libitum ◽  
Dietary Treatments

The experiment which lasted for seventy (70) days was conducted to determine the effects of various dietary levels of sunflower seed meal (SSM) on the performance of growing rabbits. Thirty- two (32) indigenous breeds of rabbits obtained from small-scale producers were used in study. They were individually weighed and randomly assigned to the four (4) dietary treatments, in which 0,10, 20 and 30% of the groundnut cake was replaced by SSM, respectively. The diets contained 2391.76, 2387.44, 2383.12 and 2378.80 Kcal ME/kg for diets 1,2,3 and 4, respectively. The crude protein (CP) was approximately 14.0% for the four (4) experimental diets. The diets, in mash form were fed to the rabbits ad libitum. There was no significant difference (P > 0.05) amongst the treatments in all the parameters examined. However, he incorporation of SSM resulted in a considerable decrease in feed cost with increasing levels of SSM. Thc. results of this Study therefore suggest that 30% or more of the GNC could be replaced by SSM in the diets of growing rabbits without deleterious effect on performance.

scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals The Effect of Using Biological Treatment on Microbial Growth during the Malting of Sorghum

2021 ◽  
pp. 105-114
Author(s):  
Oluwatosin Charles Ayodeji ◽  
Afolabi Folake Titilayo ◽  
Abdulkadir Musliu ◽  
Fasiku Oluwafemi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Starter Cultures ◽  
Polyphenol Content ◽  
Sorghum Malt ◽  
Combined Cultures ◽  
Sorghum Grain ◽  
Phytate Content

Malting is an important industrial product with a huge market outlet. Sorghum grain carries a numerous and variable, microbial population that mainly consists of bacteria, yeasts, and filamentous fungi. Sorghum malt is heavily reliant on chemical control of moulds and coliforms. This research aimed at investigating ways of improving malt quality and safety, using starter cultures of lactic acid bacteria and yeast, during the steeping stage of malting. All the steep treatments contained a sizeable population of moulds, greater than 4logcfu/mL, at 0hrs of steeping. A 3Log decrease was recorded in the steep treatment containing only single culture of Lactobacillus plantarum All the steeping treatments achieved varying levels of anti-nutrient reduction. The Lactobacillus plantarum CLB8 steep reduced the phytate level by as much as 47% when compared to the phytate level in sorghum grain. The combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiaeCYT1 reduced the phytate content by as much as 40% when compared to the sorghum grain without treatment. When compared to the control steep, the Lactobacillus plantarum CLB8 steep improved the anti-nutrient degradation by 31%. The combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiae CYT1 reduced the phytate content by as much as 23% when compared with the control steep. The polyphenol content was reduced by about 46% in the Lactobacillus plantarum CLB8 steep and 29% in the combined cultures of Lactobacillus plantarum CLB8 and Saccharomyces cerevisiae CYT1 steep when compared to the polyphenol content in the whole sorghum grain. Only the Lactobacillus plantarum CLB8 steep had better polyphenol reduction than the control with a 9.6% reduction more than the control. It was concluded that lactic acid bacteria can be apply as a biological control organism in malting of grains. 

scholarly journals Effects of Different Combination of Growing Media on Growth and Total Phenolic Content in Labisia pumila (Kacip Fatimah)

2020 ◽  
Vol 6 (2) ◽  
pp. 258-261
Author(s):  
Md. Ariff Farah Fazwa ◽  
Saffie Norhayati ◽  
Samsul Bahari Syafiqah Nabilah ◽  
Sundara Rajoo Keeren
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Growth Parameters ◽  
Abiotic Factors ◽  
Total Phenolic ◽  
Growth Media ◽  
Growing Media ◽  
Significant Difference ◽  
Labisia Pumila

Labisia pumila, locally known in Malaysia as Kacip Fatimah, is highly-prized for its phenols. Phenolic compounds in L. pumila has numerous pharmacological properties such as having anti-oxidant, anti-microbial, anti-inflammatory and anti-cancer potential. The production of phenolic contents in plants is influence by various factor, including abiotic factors such as soil properties. Thus, the objective of this study was to determine the effects of different combinations of growing media on the growth and total phenolic content in L. pumila leaves. Six combinations of growing media were used, each using different types of compost that had differing amounts of L. pumila leaves. The plants were grown in all media for a period of six months at FRIM’s nursery. Besides growth performance, the total phenolic content was also determined, six months after planting. There was no significant difference in growth parameters for all treatments, with the exception of control (significantly lower than all treatments). However, the total phenolic content of Treatment 1 (80.6 mg GAE/g) was significantly higher than all other treatments. This can be attributed to the properties of Compost 1 (which was in the growth media of Treatment 1), as it had the highest pH (5.26), the highest potassium concentration (0.7%) and high nitrogen concentrations (1.32%). The composition of Compost 1 was 5 kg dry leaves + 500 g dry Kacip Fatimah + Slow Release Fertilizer (SRF). Results from this study showed that growing media is a key factor that leads to significant increment in phenolic compounds in L. pumila.

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