scholarly journals Effect of Ulinastatin Combined with Xingnaojing Injection on Severe Traumatic Craniocerebral Injury and Its Influence on Oxidative Stress Response and Inflammatory Response

2022 ◽  
Vol 2022 ◽  
pp. 1-6
Author(s):  
Zexin An ◽  
Yong Yin ◽  
Lei Zhang ◽  
Bo Wang ◽  
Tao Cui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Inflammatory Response ◽  
Adverse Reactions ◽  
Oxidative Stress Response ◽  
Control Group ◽  
Study Group ◽  
Craniocerebral Injury ◽  
Significant Difference ◽  
Xingnaojing Injection

Objective. This study is aimed at exploring the effect of ulinastatin combined with Xingnaojing injection on severe traumatic craniocerebral injury and its influence on oxidative stress response and inflammatory response in patients. Methods. A total of 100 patients with severe traumatic craniocerebral injury admitted to our hospital from January 2018 to January 2020 were selected and equally assigned into a study group (50 cases) and a control group (50 cases) according to a random sampling method. Patients in study group received treatment of ulinastatin combined with Xingnaojing injection, while those in control group were treated with ulinastatin only. The study compared the two groups on the oxidative stress response, inflammatory response, the therapeutic effect, and the incidence rate of adverse reactions. Results. It is observed that patients in study group obtained lower levels of free cortisol (FC) and norepinephrine (NE) in the serum and higher level of total thyroxine (TT4) after treatment compared with those in control group with significant difference ( P < 0.05 ); in the meantime, they were examined to have significantly fewer oxidative stress response products, lower serum inflammatory factor level, and serum indicator levels of craniocerebral injury as opposed to those in control group, suggesting significant differences ( P < 0.05 ); study group demonstrated higher treatment response rate and lower incidence rate of adverse reactions compared with control group with a significant difference ( P < 0.05 ). Conclusion. The study found that ulinastatin combined with Xingnaojing infection has a significant effect in the treatment of severe traumatic craniocerebral injury, which can reduce the degree of craniocerebral injury and the level of inflammatory factors in the serum of patients. It is worthy of being promoted and applied clinically.

scholarly journals Ling-Gui-Zhu-Gan Decoction Protects H9c2 Cells against H2O2-Induced Oxidative Injury via Regulation of the Nrf2/Keap1/HO-1 Signaling Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Xiang Wang ◽  
Tongjuan Tang ◽  
Mengting Zhai ◽  
Ruirui Ge ◽  
Liang Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heart Failure ◽  
Stress Response ◽  
Caspase 3 ◽  
Underlying Mechanism ◽  
Oxidative Stress Response ◽  
Control Group ◽  
H9c2 Cells ◽  
Model Group ◽  
Blank Control Group

Objectives. Ling-Gui-Zhu-Gan decoction (LGZGD) is a potentially effective treatment for heart failure, and it showed significant anti-inflammatory potential in our previous studies. However, its ability to ameliorate heart failure through regulation of oxidative stress response is still unknown. This study was aimed to investigate the protective effect of LGZGD-containing serum on H2O2-induced oxidative injury in H9c2 cells and explore the underlying mechanism. Methods. Eighteen rats were randomly divided into two groups: the blank control group and LGZGD group. The LGZGD group rats were administrated with 8.4 g/kg/d LGZGD for seven consecutive days through gavage, while the blank control group rats were given an equal volume of saline. The serum was extracted from all the rats. To investigate the efficacy and the underlying mechanism of LGZGD, we categorized the H9c2 cells into groups: the control group, model group, normal serum control (NSC) group, LGZGD group, LGZGD + all-trans-retinoic acid (ATRA) group, and ATRA group. Malonedialdehyde (MDA) and superoxide dismutase (SOD) were used as markers for oxidative stress. Dichlorodihydrofluorescin diacetate (DCFH-DA) staining was used to measure the levels of reactive oxygen species (ROS). The apoptosis rate was detected using flow cytometry. The expression levels of pro-caspase-3, cleaved-caspase-3, Bcl-2, Bax, Keap1, Nrf2, and HO-1 were measured using western blotting. The mRNA levels of Keap1, Nrf2, and HO-1 were measured using RT-qPCR. Results. The LGZGD attenuated injury to H9c2 cells and reduced the apoptosis rate. It was also found to upregulate the SOD activity and suppress the formation of MDA and ROS. The expression levels of pro-caspase-3 and Bcl-2 were significantly increased, while those of cleaved-caspase-3 and Bax were decreased in the LGZGD group compared with the model group. As compared with the model group, the LGZGD group demonstrated decreased Keap1 protein expression and significantly increased Nrf2 nuclear expression and Nrf2-mediated transcriptional activity. ATRA was found to reverse the LGZGD-mediated antioxidative and antiapoptotic effect on injured H9c2 cells induced by H2O2. Conclusion. Our results demonstrated that LGZGD attenuated the H2O2-induced injury to H9c2 cells by inhibiting oxidative stress and apoptosis via the Nrf2/Keap1/HO-1 pathway. These observations suggest that LGZGD might prevent and treat heart failure through regulation of the oxidative stress response.

scholarly journals Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle

Reproduction ◽  
2013 ◽  
Vol 146 (2) ◽  
pp. 181-191 ◽  
Author(s):  
F C F Dias ◽  
M I R Khan ◽  
M A Sirard ◽  
G P Adams ◽  
J Singh
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Stress Response ◽  
Granulosa Cells ◽  
Rna Extraction ◽  
Oxidative Stress Response ◽  
Endoplasmic Reticulum Stress Response ◽  
Control Group ◽  
Matrix Remodeling ◽  
Upregulated Genes

Microarray analysis was used to compare the gene expression of granulosa cells from dominant follicles with that of those after superstimulatory treatment. Cows were allocated randomly to two groups (superstimulation and control, n=6/group). A new follicular wave was induced by ablation of follicles ≥5 mm in diameter, and a progesterone-releasing device controlled internal drug release (CIDR) was placed in the vagina. The superstimulation group was given eight doses of 25 mg FSH at 12-h intervals starting from the day of wave emergence (day 0), whereas the control group was not given FSH treatment. Both groups were given prostaglandin F2α twice, 12 h apart, on day 3 and the CIDR was removed at the second injection; 25 mg porcine luteinizing hormone (pLH) was given 24 h after CIDR removal, and cows were ovariectomized 24 h later. Granulosa cells were collected for RNA extraction, amplification, and microarray hybridization. A total of 190 genes were downregulated and 280 genes were upregulated. To validate the microarray results, five genes were selected for real-time PCR (NTS, FOS, THBS1, FN1, and IGF2). Expression of four genes increased significantly in the three different animals tested (NTS, FOS, THBS1, and FN1). The upregulated genes are related to matrix remodeling (i.e. tissue proliferation), disturbance of angiogenesis, apoptosis, and oxidative stress response. We conclude that superstimulation treatment i) results in granulosa cells that lag behind in maturation and differentiation (most of the upregulated genes are markers of the follicular growth stage), ii) activates genes involved with the NFE2L2 oxidative stress response and endoplasmic reticulum stress response, and iii) disturbs angiogenesis.

scholarly journals Clinical Effect of Auricular Point Sticking in Patients with Lung Cancer Receiving Chemotherapy

2021 ◽  
Vol 5 (6) ◽  
pp. 73-77
Author(s):  
Xiaorui Zhu
Keyword(s):  
Lung Cancer ◽  
Stress Response ◽  
Adverse Reactions ◽  
Clinical Effect ◽  
Clinical Symptoms ◽  
Auricular Point Sticking ◽  
Jiangxi Province ◽  
Control Group ◽  
Study Group ◽  
Auricular Point

Objective: To discuss and analyze the clinical effect of auricular point sticking in lung cancer chemotherapy. Methods: Sixty-two patients with lung cancer treated with chemotherapy in Suqian Traditional Chinese Medicine Hospital of Jiangxi Province were selected for case evaluation and analysis. The time span of the research was from June 2020 to June 2021. The patients were divided into two groups: a study group (n = 31) and a control group (n = 31) based on their medical record numbers. All the patients were treated with conventional western medicine before and after chemotherapy to prevent adverse reactions; however, the patients in the study group were also treated with auricular point sticking in addition to the former. The relevant indexes of the two groups were compared. Results: The incidence of adverse reactions was significantly lower in the study group compared to the control group (P < 0.05); the rate of symptomatic relief of the patients in the study group was higher than that of the control group (P < 0.05); the stress response indexes toward chemotherapy of the study group were better than those of the control group (P < 0.05). Conclusion: Auricular point sticking for patients with lung cancer who are receiving chemotherapy can reduce the incidence of adverse reactions, alleviate clinical symptoms, such as chest distress, asthma, and poor appetite, significantly alleviate stress response caused by chemotherapy, as well as promote the treatment effect; thus, it is worthy of promotion.

scholarly journals Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242789
Author(s):  
Yoko Ito ◽  
Kana Oshinden ◽  
Naokata Kutsuzawa ◽  
Chinatsu Kohno ◽  
Sanae Isaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Epithelial Cells ◽  
Oxidative Stress Response ◽  
Alveolar Epithelial Cells ◽  
Target Cells ◽  
Type I ◽  
Alveolar Epithelial ◽  
Significant Difference ◽  
Different Types

There has been an increase in the usage of heat-not-burn (HNB) cigarette products. However, their effects on alveolar epithelial cells (AECs) remain unknown. AECs are the target cells of conventional cigarette smoking-related respiratory diseases such as chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis and lung cancer whose pathogenesis involves oxidative stress. In this study, primary rat AECs were isolated, cultured and stimulated by HNB cigarette smoke extract (CSE). Our data indicate that rat AECs exposed to HNB CSE induced oxidative stress response genes (e.g. Hmox-1, Gsta1, Gsta3 and Nqo1). We also compared the oxidative stress response between two different types of AECs, alveolar type I-like (ATI-like) cells and type II (ATII) cells, and between two different types of cigarette, HNB cigarettes and conventional cigarettes. The expressions of Gsta1, Gsta3 and Nqo1 were higher in ATII cells than ATI-like cells in response to HNB and conventional cigarettes, but there was no significant difference in their expression levels between HNB cigarette and conventional cigarette. Taken together, our results suggest that HNB cigarettes have the similar potential as conventional cigarette products to induce oxidative stress response in AECs.

scholarly journals Effect of dexmedetomidine on oxidative stress response and expression of intracellular adhesion factor-1 (ICAM-1) and S100B in patients

2021 ◽  
Keyword(s):  
Oxidative Stress ◽  
Brain Injury ◽  
Stress Response ◽  
Treatment Group ◽  
Oxidative Stress Response ◽  
Stress Index ◽  
Control Group ◽  
Sod Activity ◽  
Gcs Score ◽  
Adhesion Factor

[Abstract] Objective: To study the effect of dexmedetomidine on oxidative stress response and the expression of intracellular adhesion factor-1 (ICAM-1) and S100B in patients with traumatic brain injury (TBI). Method: TBI patients treated in our hospital from May 2017 to April 2019 were enrolled in the study and divided into control group and treatment group by random number table method. The treatment group was administered with dexmedetomidine injection via intravenous pump on the basis of conventional treatment in the control group. Glasgow coma scale (GCS) and Glasgow outcome scale (GOS) were used to evaluate patients’ injury, recovery and prognosis. ELISA method was taken to detect 4 oxidative stress index levels including serum superoxide dismutase (SOD), lipid peroxidation (LPO), malondialdehyde (MDA) and total antioxidant capacity (TAC), as well as ICAM-1 and S100B levels at admission and at different time points after operation. Results: At 3d and 14 d after operation, the treatment group had higher GCS score than the control group (P<0.05).At 30 d, 90 d and 180 d after discharge, the treatment group had higher GOS score than the control group (P<0.05). At 3d, 5d, and 14d after operation, the treatment group had higher SOD activity than the control group (P<0.05). Immediately after operation, at 3d, 5d, and 14d after operation, the treatment group had higher LPO level than the control group (P<0.05); at 3d, 5d, and 14d after operation, blood MDA level gradually decreased in both groups, which was lower in the treatment group than in the control group (P<0.05); at 3d, 5d and 14d after operation, the treatment group had higher TAC activity in the blood than the control group (P<0.05). At 3d, 5d and 14d after operation, the treatment group had lower S100B level than the control group (P<0.05). Conclusion: Dexmedetomidine can relieve TBI-induced oxidative stress state and reduce the levels of brain injury markers (ICAM-1, S100B), which has a protective effect on the brain tissue with TBI.

scholarly journals Single-Wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus Praecox Embryogenic Callus

2020 ◽  
Author(s):  
Li Ren ◽  
Shan Deng ◽  
Yunxia Chu ◽  
Yiying Zhang ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Survival Rate ◽  
Stress Response ◽  
Embryogenic Callus ◽  
Oxidative Stress Response ◽  
Single Wall Carbon Nanotubes ◽  
Control Group ◽  
Single Wall Carbon ◽  
Agapanthus Praecox

Abstract Background: Cryopreservation is the best way for long-term preservation of plant germplasm resources. The preliminary studies found that ROS-induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration, and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during dilution step. Combined with the physiological and quantitative gene expression results, SWCNTs affect ROS signal transduction and the antioxidant system in plant cryopreservation. The EC treated with SWCNTs exhibited higher antioxidant levels, including POD, CAT and GSH than the control group EC. EC mainly depended on AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. Elevated antioxidant status in dehydration by addition of SWCNTs enhanced cells resistance to cryoinjury. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.Conclusions: SWCNTs regulated the oxidative stress response of EC during cryopreservation, and controlled cell oxidative damage via the maintenance of ROS homeostasis to achieve high survival rate after cryopreservation. This study is the first time to systematically describe the role of carbon nanomaterials in the regulation of plant oxidative stress response, and provided a novel insight into cell stress response mechanisms in cryopreservation.

scholarly journals Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

2017 ◽  
Vol 38 (3) ◽  
pp. 393-408 ◽  
Author(s):  
Nedelina Kostadinova ◽  
Solveig Tosi ◽  
Boryana Spassova ◽  
Maria Angelova
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Growth Temperature ◽  
Growth Conditions ◽  
Oxidative Stress Response ◽  
Optimal Temperature ◽  
Sod Activity ◽  
Glycogen Accumulation ◽  
Significant Difference ◽  
Different Temperatures

Abstract Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below – (10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below– and above–optimum caused strain–dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below– and above–optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.

The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress

Perfusion ◽  
2009 ◽  
Vol 24 (4) ◽  
pp. 231-237 ◽  
Author(s):  
N. Sohn ◽  
J. Marcoux ◽  
T. Mycyk ◽  
J. Krahn ◽  
QH Meng
Keyword(s):  
Oxidative Stress ◽  
Cardiopulmonary Bypass ◽  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Artery Bypass ◽  
Serum Levels ◽  
Control Group ◽  
Significant Difference ◽  
The Impact ◽  
And Oxidative Stress

This study was to compare the impact of different biocompatible coated circuits on inflammatory response and oxidative stress induced during cardiopulmonary bypass (CPB). Seventy-eight patients undergoing elective coronary artery bypass grafting (CABG) with CPB were randomly assigned to five groups with different biocompatible coated circuits: Trillium, Bioline, Phosphorylcholine, Polymethoxyethyl acrylate (PMEA), and the uncoated control group. Blood was drawn at three different time points: before CPB, 6 and 72 hours post CPB. Unlike the Trillium group, serum levels of TNF-α in the Bioline and Phosphorylcholine groups significantly increased only at 72 hours post CPB (p < 0.05). Serum levels of IL-6 significantly increased at 6 and 72 hours post CPB in all groups (p < 0.01). The Trillium group showed a significant increase of IL-10 compared to the control group at 72 hours post CPB (p < 0.05). Serum levels of NOx in the Phosphorylcholine group significantly decreased at 6 hours post CPB compared to baseline (p < 0.05). Both the Bioline and Phosphorylcholine groups showed statistical decreases in serum NOx levels compared with other groups at 6 hours post CPB (p < 0.05). A significant difference in NOx levels between the Bioline and the control group was also observed at 72 hours post CPB. Myeloperoxidase levels were significantly elevated at 6 and 72 hours post CPB in all groups (p < 0.05). Inflammatory response and oxidative stress are elevated during CABG with CPB. Heparin-coated and the Phosphorylcholine-coated circuits induce less inflammatory responses and oxidative stress compared to other circuits.

scholarly journals Analysis of the Value of Elojumab in Patients with Acute Coronary Syndrome after Percutaneous Coronary Intervention

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Baojun Fu ◽  
Daqing Song ◽  
Shuyin Sun
Keyword(s):  
Percutaneous Coronary Intervention ◽  
Acute Coronary Syndrome ◽  
Adverse Reactions ◽  
Coronary Intervention ◽  
Control Group ◽  
Study Group ◽  
Coronary Syndrome ◽  
Cerebrovascular Events ◽  
Percutaneous Coronary ◽  
Significant Difference

Objective: To analyze the application value of Elouzumab in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI), so as to lay a foundation for the follow-up treatment. Methods: 84 ACS patients who underwent PCI in our hospital from December 1, 2018 to December 1, 2019 were selected and divided into control group (n = 42) and study group (n = 42) according to the random number table. The control group was treated with statins, and the study group was treated with alloxan combined therapy. The changes of blood lipid index, quality of life score, adverse cardiovascular and cerebrovascular events and adverse reactions were compared before and after treatment. Results: There was no significant difference in TCHO, TG, HDL-C and LDL-C between the two groups before treatment (P>0.05);After treatment, the levels of TCHO, TG, HDL-C and LDL-C in the study group were significantly lower than those in the control group (P<0.05);There was no significant difference in the scores of WHOQOL-BREF before treatment (P>0.05);After treatment, the WHOQOL-BREF scores of the two groups were improved, and the study group was significantly higher than the control group (P<0.05);The incidence of adverse cardiovascular and cerebrovascular events and adverse reactions in the study group was lower than that in the control group, but the difference was not statistically significant (P>0.05). Conclusion: After percutaneous coronary intervention in patients with acute coronary syndrome, the use of Elojumab can effectively reduce the blood lipid index, improve the quality of patients and reduce the incidence of adverse cardiovascular and cerebrovascular events and adverse reactions, which can be effectively promoted in clinical practice.

scholarly journals “Free radical induced oxidative stress” (frios) parameters: key to reduce feto-maternal mortality in high risk pregnancies

2018 ◽  
Vol 7 (7) ◽  
pp. 2840
Author(s):  
Shalini Chauhan ◽  
Kiran Pandey
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
High Risk ◽  
Maternal Mortality ◽  
Control Group ◽  
Study Group ◽  
Term Infants ◽  
Super Oxide Dismutase ◽  
Significant Difference ◽  
The Mean

Background: ROS plays role during pregnancy and normal parturition and in recurrent pregnancy loss, initiation of preterm labor, anaemia, preeclampsia, eclampsia, Intrauterine growth retardation. Elevated oxidative stress is found in term infants with fetal distress and in preterm infants. With this background this study was conducted to evaluate the role of FRIOS (free radical induced oxidative stress) in reducing feto -maternal mortality in high risk pregnancies in District Kanpur.Methods: This prospective study was conducted on pregnant women at high risk attending department of Obstetrics and Gynaecology, GSVM Medical College, Kanpur after taking permission from the institutional ethical committee. Informed consent was obtained from all patients. Investigations were carried out to measure oxidant level of Malonaldialdehyde (MDA) enzyme & to measure anti oxidant Super Oxide Dismutase (SOD) enzyme. Levels of these enzymes were compared between normal & each high risk sub groups separately. SPSS software was used for statistical analysis and suitable tests were applied.Results: The mean value in study group was highest in severely anaemic patients (8.53±1.398 Nmoles/ml of plasma) followed by pre eclamptic & eclamptic patients (8.33±1.355 Nmoles/ml of plasma). The mean levels of in study group was lowest in pre eclamptic & eclamptic patients (0.394±.191 u/mg of protein) followed by pre term patients (0.413±0.141 u/mg of protein). Significant difference between MDA enzyme levels in control group and all sub groups of study group was found.Conclusions: Measurement of Malonaldialdehyde (MDA) & Super Oxide Dismutase (SOD) enzymes at an earlier stage can be a valuable tool for early diagnosis, so that we can timely intervene & improve the maternal outcome.

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