scholarly journals The Effect of Golden Sea Cucumber (Stichopus hermanii) Extracts to Serum LDL Cholesterol level as a Result of Insulin Resistence

2020 ◽  
Vol 1 (1) ◽  
pp. 7-12
Author(s):  
Herin Setianingsih ◽  
Fatima Sari Suryono Putri ◽  
Wahyu Prasasti Mutia Desi ◽  
Erlina Yatnasari
Keyword(s):  
Sea Cucumber ◽  
Ldl Cholesterol ◽  
Design Method ◽  
White Male ◽  
Simple Random Sampling ◽  
Serum Levels ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Cholesterol Levels

ABSTRACT Diabetes mellitus is a metabolic disease characterized by conditions of hyperglycemia as a result of abnormalities in the secretion of the insulin hormone, the work of the insulin hormone, or both. Metabolic disorders caused by diabetes cause lipolysis to increase, thereby increasing the level of VLDL, which will be converted to LDL. LDL disorders are the effects of insulin resistance. Streptozotocin (STZ) can increase serum LDL cholesterol serum levels due to the mechanism of ROS formation. Golden sea cucumber act as anti-hyperlipidemic because they contain EPA-DHA, saponins, and flavonoids. The purpose of these studies to determine the effect of Golden sea cucumber act as anti-hyperlipidemic. Experimental laboratory research is done using the post-test only control group design method. Using simple random sampling with 25 white male Wistar rats divided into five groups, all rats were given standard feeding. Group 1 without STZ induced, group 2-5 were induced STZ first, and then group 3-5 were given golden sea cucumber extract for 21 days with dose 4,25 mg/kg BW, 8,5 mg/kg BW, 12,75 mg/kg BW, per day respectively. At the end of the study, the LDL serum level was measured. The result of One – Way Anova test showed LDL cholesterol serum levels of group rats with STZ induction and given 12,75 mg/kg BW golden sea cucumber extracts (x=7,4 mg/dl) significantly decreased (p=0,005) compared to LDL cholesterol levels of group rats which were only induced by STZ without given golden sea cucumber extracts (x=12,6 mg/dl). This research showed that the administration 12,75mg/kg BW of golden sea cucumber extracts for 21 days reduced serum LDL cholesterol levels because golden sea cucumber contains compounds that work as antioxidants.

scholarly journals PERBEDAAN KADAR KOLESTEROL LDL DARAH TIKUS SPRAGUE DAWLEY PADA PEMBERIAN KOPI FILTER DAN TANPA FILTER

2012 ◽  
Vol 1 (1) ◽  
pp. 72-77
Author(s):  
Raysa Tanjung Sari ◽  
Niken Puruhita
Keyword(s):  
Ldl Cholesterol ◽  
Simple Random Sampling ◽  
Control Group ◽  
Chlorogenic Acids ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Filtered Coffee

Background : Coffee contains cafestol and kahweol which can increase LDL cholesterol levels. Effect of cafestol and kahweol with chlorogenic acids are antagonist in coffee. Chlorogenic acids is strong antioxidant in coffee. Unfiltered coffee contains more chlorogenic acids and cafestol than filtered coffee. The study aims to determine the differences of LDL cholesterol levels of Sprague Dawley rats in giving filtered and unfiltered coffee. Methods : a laboratory experimental study, with randomized pre-post test control group design, with a simple random sampling to 28 Sprague Dawley rats were divided into 3 groups. The first treatment was given unfiltered coffee 4x2.7 ml, the second treatment was given filtered coffee 4x2.7 ml and the control was given water 4x2.7 ml for 28 days. LDL cholesterol levels were determined by the homogenous enzyme method. Data were analyzed by paired t-test and Oneway ANOVA test at 95% confident level. Results : Changes levels of LDL cholesterol in filtered coffee (0.85 mg/dl) are higher than unfiltered coffee (0.15 mg/dl). However statistical test shows there is no significant difference of LDL cholesterol in filtered and unfiltered coffee (p>0.05). Conclusion : There is no significant difference of LDL cholesterol levels of Sprague Dawley rats in filtered and unfiltered coffee.

scholarly journals PERBEDAAN EFEKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorriza Roxb.) DENGAN EKSTRAK DAUN SALAM (Eugenia polyantha Wight) PADA PENURUNAN KADAR KOLESTEROL TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayu Ulan Rizki ◽  
Cholid Cholid ◽  
Muttia Amalia
Keyword(s):  
Total Cholesterol ◽  
Leaf Extract ◽  
Disease Risk ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Standard Drug ◽  
Cholesterol Levels ◽  
White Rats ◽  
Ginger Rhizome

Dyslipidemia is a coronary heart disease risk factor. Dyslipidemia is characterized by the elevated levels of LDL, triglycerides, total cholesterol, and the decrease in HDL. Ginger rhizome (Curcuma xanthorriza Roxb.) and bay leaf (Eugenia polyantha Wight.) are local plants that have the abilities to decrease total cholesterol. This research design was true experimental with pre- and post-control group design. Criteria of sample were white rats, wistar strain, male, age of 8-12 weeks, and body weight range of 150-200 grams. Sampling method was simple random sampling. The experimental rats were acclimatized for seven days. Hypercholesterol diet was fed two weeks, and then the rats were given intervention and get hypercholesterol diet for two weeks. During intervention, the experimental rats were divided into 6 groups of administrationi.e, rats administered with 1) carboxymethil celluse/CMC, 2) simvastatin 0,9 mg/kg BW/day, 3) ginger rhizome extract 200 mg /kg BW/day, 4) ginger rhizome extract 400 mg/kg BW/day, 5) bay leaf extract 200 mg/kg BW/day, and 6) bay leaf extract 400 mg/kg BW/day. Blood samples were taken from caudal vein and total cholesterol levels were measured by spectrophotometry. The analysis used paired T test, One Way ANOVA test. The results showed ginger rhizome extract did not effectively reduce total cholesterol. However, bay leaf extract effectively decreased total cholesterol levels with the highest total cholesterol reduction was found in rats administered with dose of 400 mg/kg BW/day i.e, 15.4 mg/dl. Bay leaf extract have the same effectivity with simvastatin as a standard drug in lowering cholesterol level.

scholarly journals PENGARUH PEMBERIAN KEFIR SUSU SAPI TERHADAP KADAR KOLESTEROL LDL TIKUS JANTAN SPRAGUE DAWLEY HIPERKOLESTEROLEMIA

2012 ◽  
Vol 1 (1) ◽  
pp. 358-364 ◽  
Author(s):  
Sandy Eka Pratama ◽  
Enny Probosari
Keyword(s):  
Ldl Cholesterol ◽  
Direct Method ◽  
Wilcoxon Test ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
Sprague Dawley Rats ◽  
Post Test ◽  
Cause Of Deaths

Background: Hypercholesterolemia is a main risk factor of cardiovascular disease that remains the higher cause of deaths in the world. Milk kefir contains lactic acid bacteria which can reduce LDL cholesterol. The objective of this study was to determine the effect of milk kefir on LDL cholesterol levels in male Sprague dawley hypercholesterolemia rats. Methods: This research was true-experimental using pre-post test with randomized control group design. Subjects were male Sprague dawley rats, inducted hypercholesterolemia, given milk kefir diet using 1,5 ml, 2 ml, and 3 ml dosage for 15 days. The LDL cholesterol levels were measured by direct method using homogenous enzymatic LDL cholesterol. Normality of the data were tested by Shapiro Wilk test. Data were analyzed by Wilcoxon test and Kruskall-Wallis continued by Mann-Whitney test. Result: The study revealed that milk kefir of dosage 2 ml/day decreased LDL cholesterol significantly (p<0,05)  from 196,57 ± 84,11 mg/dl to 38,95 ± 18,22 mg/dl and also for 3 ml/day decreased LDL cholesterol significantly (p<0,05) from 100,14 ± 36,20 mg/dl to 26,91 ± 9,93 mg/dl. Other dosage of 1,5 ml/day is not significantly decrease LDL cholesterol level. Conclusion: Milk kefir able to reduce LDL cholesterol levels in all groups. By giving mik kefir 2 ml/day and 3ml/day during 15 days able to  reduce LDL cholesterol levels 80,18% and 73,12%.

scholarly journals Decrease of LDL Cholesterol through Increase of HDL Cholesterol by Administering Garcinia mangostana L. Peel Extract in White Mice

2021 ◽  
Vol 57 (4) ◽  
pp. 298
Author(s):  
Hasyim As'ari
Keyword(s):  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Negative Control ◽  
Laboratory Research ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Significance Level ◽  
High Fiber ◽  
Peel Extract

Highlight:Ethanol extract of Garcinia mangostana L. peel reduce malondialdehyde.Garcinia mangostana L. peel extract can increase non-radical products which impact in decreasing LDL cholesterol and increasing HDL cholesterol. Abstract:Atherosclerosis contributes to coronary heart disease which may lead to fatality. High cholesterol consumption, stress, and smoking can increase LDL cholesterol in the blood. Consumption of unsaturated fats, high fiber foods, exercise, quitting smoking, losing weight, and giving hypolipidemic drugs, especially herbs, can increase HDL cholesterol and decrease LDL cholesterol. Garcinia mangostana L. peel  extract can decrease LDL cholesterol by increasing reverse HDL cholesterol transport to the liver. The study used post test control group design. This study was experimental laboratory research with population of hypercholesterolemic male white mice aged 3-4 weeks with 100-200 grams weight. The HDL and LDL cholesterol data were collected through an enzymatic method by spectrophotometer. This study used analysis of variance (Anova) with significance level of α <0.05. The experiment divided the subjects into positive and negative control groups with dosage variations of 50, 150, 250, and 350 mg/kgBW.  Examination of hypercholesterolemia in white mice was conducted on the 8th day. The examination of HDL and LDL cholesterol given peel extract of Garcinia mangostana L. was conducted on the 22nd day. The analysis showed that giving Garcinia mangostana L. peel extract for various dosages could significantly decrease LDL cholesterol and increase HDL cholesterol (p <0.05). Peel extract of Garcinia mangostana L. that contained mangosteen could increase non-radical products that could prevent the transfer of ester cholesterol from HDL to VLDL which impact in increasing HDL cholesterol and decreasing LDL cholesterol.

scholarly journals THE EFFECT OF GIVING BENGKOANG JUICE (PACHYRRHIZUS EROSUS) ON BLOOD CHOLESTEROL LEVELS AND COMFORT COMPLAINTS TO MENOPAUSE WOMEN IN BENGKULU CITY

2020 ◽  
Vol 9 (1) ◽  
pp. 37-44
Author(s):  
Kurnia Dewiani
Keyword(s):  
Sampling Technique ◽  
Simple Random Sampling ◽  
Study Groups ◽  
Statistical Testing ◽  
Blood Cholesterol ◽  
Control Group ◽  
P Value ◽  
Menopausal Syndrome ◽  
Control Group Design ◽  
Cholesterol Levels

Most of the women have been experienced with menopausal syndrome 70-80 %. Estrogen and progesterone hormones activity decrease which the result of the cessation of menstruation followed by various physical and psychological changes. Blood cholesterol levels and complaints of discomfort are the effects of pre and menopause. Reducing the effect of menopause could be done with non-pharmacologically by utilizing natural materials that are easily available, cheap and minimal negative side effects, namely by consuming Bengkoang juice regularly for 21 days. This research aims to recognize the effect of giving Bengkoang juice by cholesterol levels and complaints of discomfort during menopause This research was a quasi-experimental pre-posttest with control group design. 30 pre and menopausal women participated in their resident of Tanah Patah Village, Bengkulu City with a purposive sampling technique was divided into two groups by simple random sampling. Each group was consisting of 15 women for treatment (Bengkoang juice) and control (soy milk). Assessment of cholesterol levels through an examination used a stick of cholesterol (easy touch), complaints used the questionnaire complaints, and respondents' compliance used the observation sheet. Statistical testing was applied paired T-Test analysis to see differences in cholesterol levels and complaints of discomfort between the two study groups with the significance of the test results based on p <0.05. The results present a decrease in cholesterol levels and complaints in both groups with a p-value <0.05. Furthermore, in terms of decreased levels of cholesterol (33%) and complaints (45%) were higher than the control group namely cholesterol levels (11%) and complaints ( 21%). This research concluded that the effect of Bengkoang juice on cholesterol levels and complaints of discomfort during menopause is confirmed.

PENGARUH PRACTICE BASED SIMULATION MODEL TERHADAP CRITICAL THINKING DI NURSING SKILL LABORATORY

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Retno Issroviatiningrum ◽  
Shanti Wardaningsih ◽  
Novita Kurnia Sari
Keyword(s):  
Critical Thinking ◽  
Simulation Model ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Skill Laboratory ◽  
Paired T Test

ABSTRAK Upaya untuk meningkatkan mutu pendidikan keperawatan khususnya untuk mendukung peserta didik menjadi perawat profesional memerlukan proses pembelajaran dengan menggunakan fasilitas keterampilan klinis. Practice based simulation model didasarkan pada teori belajar konstruktif yang menegaskan bahwa pengetahuan tidak pasif ditransfer dari pendidik kepada peserta didik, tetapi dibangun oleh peserta didik melalui pengolahan pengalaman dan interaksi dengan lingkungan mereka. Dengan metode simulasi di laboratorium dapat mendorong mahasiswa untuk menggunakan critical thinking dalam mengambil keputusan dalam mengatasi masalah tanpa merugikan pasien yang sebenarnya. Penelitian ini bertujuan untuk menganalisis pengaruh practice based simulation model terhadap critical thinking pada mahasiswa semester VI di FIK Unissula Semarang. Penelitian ini menggunakan metode Quasy – Experiment dengan pendekatan pretest-posttest with control group design. Pengambilan sampel dengan teknik  simple random sampling dengan jumlah 21 responden baik kelompok intervensi maupun kelompok kontrol. Analisis data menggunakan uji Paired T-Test dan Independent Samples T-Test. Hasil penelitian selisih peningkatan critical thinking pada kelompok intervensi sebanyak 11.95 poin dan pada kelompok kontrol 2.05. Practice based simulation model berpengaruh terhadap critical thinking dengan nilai p=0.00<0.05. Disimpulkan bahwa practice based simulation model mempengaruhi critical thinking pada mahasiswa semester VI FIK Unissula Semarang. Kata kunci: Practice based simulation model, critical thinking

PENGGUNAAN MODEL PEMBELAJARAN STAD (STUDENT TEAMS-ACHIEVMENT DIVISIONS) DALAM PEMBELAJARAN MENULIS SURAT DINAS SISWA KELAS VIII DI SMP NEGERI SE-KECAMATAN PANYINGKIRAN KABUPATEN MAJALENGKA TAHUN AJARAN 2013/2014

2017 ◽  
Vol 3 (1) ◽  
pp. 447
Author(s):  
Iyay Robia Khoerudin ◽  
Neneng Titin ◽  
Eki Kiyamudin
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Student Teams ◽  
Post Test ◽  
Test Control

Penelitian ini bertujuan untuk mengetahui kemampuan menulis surat dinas siswa kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka, mengetahui kemampuan menulis surat dinas dengan menggunakan model pembelajaran STAD (Student Teams-Achievment Divisions) siswa kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka, serta menjelaskan efektifitas model pembelajaran STAD (Student Teams-Achievment Divisions) untuk meningkatkan  pengajaran menulis surat dinas siswa Kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka.Penelitian ini menggunakan pendekatan kuantitatif dan kualitatif dengan metode eksperimen Pre test-Post test Control Group Design, populasinya yaitu SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka tahun ajaran 2013/2014, sedangkan  sampelnya ditentukan melalui teknik simple random sampling yaitu kelas VIII G SMP Negeri 2 Panyingkiran sebagai kelas eksperimen dan kelas VIII B SMP Negeri 1 Panyingkiran sebagai kelas kontrol. Instrumen pengumpulan data yang digunakan tes tertulis tes awal dan tes akhir, kuesioner, lembar observasi guru dan siswa, setelah data diperoleh maka dilakukan analisis data. Data kuesioner dan observasi dianalisis dalam bentuk uraian sedangkan data tes dianalisis dengan statistik dan di deskripsikan.Berdasarkan perhitungan statistik program SPSS 16.0 diperoleh hasil uji t pretes pada kedua kelas tersebut memiliki nilai t=5.429 derajat kebebasan (df)=n-1=38 nilai probability (sig 2-tailed) sebesar 0,000.  Hal ini berarti t hitung <0,005. Artinya 0,000<0,05, pada uji t ini bahwasanya pretes pada kedua kelas terdapat perbedaan yang signifikan antara kelas eksperimen dengan kelas kontrol.  Apabila ditinjau dari segi rata-rata pretes eksperimen lebih efektif daripada kelas kontrol. Hal ini terbukti dari rata-ratanya pretes kelas eksperimen 64,8 kelas kontrol 62,3 selisih rata-rata 2,5. Sedangkan rata-rata postes kelas eksperimen 81,2 dan kelas kontrol 69 terjadi selisih postes 12,2.

Efektivitas Model Pembelajaran Kooperatif Tipe Make-A Match Dalam Meningkatkan Kompetensi Sikap Siswa dan Kompetensi Pengetahuan Siswa Pada Pelajaran IPS

2020 ◽  
Vol 4 (1) ◽  
pp. 80
Author(s):  
Dina Anika Marhayani ◽  
Fajar Wulandari
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Penelitian ini bertujuan untuk mengetahui keefektifan model pembelajaran kooperatif tipe  Make- A Match terhadap: (1) kompetensi sikap siswa, (2) kompetensi pengetahuan siswa pada pelajaran IPS di kelas V SD N 1  Singkawang. Jenis penelitian ini adalah penelitian quasi eksperimen dengan desain pretest-posttest control group design. Teknik simple random sampling dilakukan untuk menentukan kelas eksperimen dan kontrol. Kelas eksperimen yakni V C diberi perlakuan dengan menggunakan model pembelajaran kooperatif tipe Make-A Match. Kelas kontrol V A diberi perlakuan dengan menggunakan metode ceramah dan diskusi. Pengumpulan data menggunakan tes, penilaian diri dan penilaian teman sejawat. Hasil penelitian menunjukkan bahwa model pembelajaran kooperatif tipe Make- A Match efektif terhadap: (1) kompetensi sikap siswa sebesar 0,016 <0,05, (2) kompetensi pengetahuan siswa pada kelas eksperimen diperoleh rata-rata N gain sebesar 61 % atau (0,61). Maka, model pembelajaran kooperatif tipe Make- A Match efektif terhadap hasil belajar siswa dilihat dari ranah kompetensi sikap dan pengetahuan. 

Ekstrak Ethanol Kulit Jeruk Purut (Citrus hystrix) Berpotensi sebagai Agen Penurun Kolesterol : Studi In Vivo

2020 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Henas Deliara ◽  
Arum Kartikadewi ◽  
Dyah Mustika Nugraheni
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Latar Belakang: Hiperkolesterolemia dapat menyebabkan penyakit cerebrovaskuler bahkan kematian. Salah satu pencegahan hiperkolesterolemia adalah dengan pemberian suplemen contohnya menggunakan kulit jeruk purut (Citrus hystrix) yang mengandung saponin, tanin dan flavonoid. Tujuan penelitian ini adalah membuktikan adanya pengaruh pemberian ekstrak ethanol kulit jeruk purut terhadap kadar kolesterol total pada tikus wistar jantan yang diberi diet tinggi lemak. Metode: Penelitian eksperimental  ini menggunakan metode post test only control group design. Jumlah sampel 30 ekor tikus wistar jantan dikelompokkan secara simple random sampling menjadi kelompok kontrol negative (K-), kontrol positif (K+), perlakuan 1 (P1), perlakuan 2 (P2) dan perlakuan 3 (P3). Pada kelompok K+, P1,P2 dan P3 tikus diberi minyak babi sebanyak 3 mg/200grBB/hari selama 3 minggu. Kelompok P1, P2 dan P3 diberikan ekstrak ethanol kulit jeruk purut sebesar 35 mg/200grBB/hari, 70 mg/200grBB/hari, dan 140 mg/200grBB/hari selama 3 minggu. Kadar kolesterol total dihitung dengan metode CHOD-PAP lalu dianalisis secara statistic dengan uji One Way Annova dan uji beda dengan Pos Hoc. Hasil: Dari 25 sampel, rerata kadar kolesterol total (K-):73,90 mg/dl±19,11 mg/dl; (K+):80,00 mg/dl±4,72 mg/dl; (P1):69,94 mg/dl±6,52 mg/dl; (P2):59,10 mg/dl±11,70 mg/dl; (P3):59,74 mg/dl±7,52 mg/dl. (p=0,032). Hasil uji Pos Hoc kelompok yang berbeda signifikan adalah K- dengan P2 (p=0,049), K+ dengan P2 (p=0,008) dan K+ dengan P3 (p=0,010). Kesimpulan: Terdapat pengaruh pemberian ekstrak ethanol kulit jeruk purut terhadap kadar kolesterol total pada tikus wistar jantan yang diberi diet tinggi lemak. Dosis terendah ekstrak kulit jeruk purut yang dapat menurunkan kadar kolesterol total adalah 70 mg/200 grBB/hari.

Pengaruh Konsumsi Cookies Kerang Dara (Anadara Granosa) terhadap Perubahan Kadar Haemoglobin Wanita Usia Subur

2020 ◽  
Vol 7 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Vera Iriani Abdullah ◽  
C.H Haumahu
Keyword(s):  
Data Collection ◽  
Perinatal Death ◽  
Design Method ◽  
Statistical Tests ◽  
Total Sample ◽  
Control Group ◽  
Childbearing Age ◽  
Control Group Design ◽  
Quasi Experimental ◽  
Anadara Granosa

In the world, around 1.62 billion people have low HB levels, around 30.2% occur in the group of women aged 15-49. In Indonesia, anemia cases rank 4th in the top 10 most disease groups. Prolonged iron deficiency can cause anemia, so it needs to be treated immediately so it doesn’t continue into pregnancy age which can cause complications until maternal and perinatal death. Papua has an abundance of marine wealth; one of those is Kerang Dara. Through this study, researchers wanted to explore the health benefits of nature. The aim is to see the effect of Consumption of Kerang Dara Cookies (Anadara Granosa) on Changes of Hemoglobin Levels of in Woman of Childbearing Age as an Effort to Prevent Anemia in District Aimas, Klaigit Village. Type of this research is quasi-experimental by pretest-posttest control group design method. The population of all the women of Childbearing who live in Klaigit Village, with a total sample of 14 people divided into 2 groups: control and intervention. Data collection techniques using random sampling. The time of data collection occurred for 2 weeks, starting from September 20th to October 4th. The results based on statistical tests using T-Test, then the value 884 is greater than the value of the table 0.05, then the conclusion is there is an Effect of Consumption Kerang Dara Cookies (Anadara Granosa) Towards the Increased of Woman of Childbearing Hemoglobin Levels in Klaigit Village in District Aimas in 2019.

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