Effect of Administration of CHAGURO Made of Chayote (Sechium edule) and Tuna (Thunnus sp.) on Rats Induced with Streptozotocin-Nicotinamide and a High-Fat Diet

This study was conducted to develop Chaguro, a low-cost supplementary food made of chayote (Sechium edule (Jacq.) Swartz) and tuna fish (Thunnus sp.), for diabetes and dyslipidemia diet therapy. In order to find a formula with effective hypoglycaemic and antidyslipidemic properties, dried tuna and chayote were mixed at different ratios: F1 (75% tuna, 25% chayote), F2 (50% tuna, 50% chayote), and F3 (25% tuna, 75% chayote). Thirty male Sprague Dawley rats were assigned into healthy control group or groups induced with streptozotocin-nicotinamide and a high-fat diet. Chaguro was administered 2.7 g/ kgBW/ day using a gavage for 28 days. The administration of all Chaguro formulas improved blood markers compared to the negative control group (p < 0.001). Chaguro F2 lowered fasting blood glucose (97.07±1.18 vs 266.31±5.31), total cholesterol (113.59±2.22 vs 208.78±4.31), triglycerides (89.93±2.51 vs 142.35±2.83), LDL-c (33.87±1.87 vs 87.85±3.34) and increased HDL-c (69,08±1,85 vs 23,91±1,64) level the most compared to the negative control group (p < 0.001). Streptozotocin-induced weight loss was also prevented in all diabetic rats fed with Chaguro, with the bodyweight being similar to that of healthy controls at the end of the intervention (p < 0.001). This study found that Chaguro may be a potential food product to help lower blood glucose and improve lipid profile in diabetes and dyslipidemia.

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The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed High-fat Diet or a High-fat Diet Combined with STZ Treatment

10.20944/preprints201911.0343.v1 ◽

2019 ◽

Author(s):

Zhen-hong Xia ◽

Wen-bo Chen ◽

Li Shi ◽

Xue Jiang ◽

Ke Li ◽

...

Keyword(s):

High Fat Diet ◽

Curcuma Longa ◽

Food Pellet ◽

Fasting Blood Glucose ◽

Density Lipoprotein ◽

Diet Group ◽

Lipoprotein Cholesterol ◽

Control Group ◽

Sprague Dawley ◽

High Fat

Curcumin is the main secondary metabolites of Curcuma longa and other Curcuma spp, and has been reported to have some potential in preventing and treating some physiological disorders. This study investigated the effect curcumin in inhibiting high-fat diet and streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Twenty-six male Sprague-Dawley (SD) rats (170-190 g) were randomly divided into a standard food pellet diet group (Control group), a high-fat diet and streptozotocin group (HF+STZ group), and a high-fat diet combined with curcumin and STZ group (HF+ Cur +STZ group). Compared with the HF+STZ group, the HF+Cur+STZ group exhibited significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (AST) and aspartate transaminase (ALT) levels, and liver coefficients; in the livers of these rats, the expression of malondialdehyde (MDA) and Bax was downregulated, whereas that of superoxide dismutase (SOD) and Bcl-2 was upregulated. Moreover, the liver histology of these rats was improved and resembled that of the control rats. These results suggest that curcumin prevents high-fat diet and STZ-induced hyperglycemia and hyperlipidemia, mainly via anti-oxidant and anti-apoptotic mechanisms in the liver.

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The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed a High-Fat Diet Combined With STZ Treatment

Molecules ◽

10.3390/molecules25020271 ◽

2020 ◽

Vol 25 (2) ◽

pp. 271 ◽

Cited By ~ 1

Author(s):

Zhen-Hong Xia ◽

Wen-Bo Chen ◽

Li Shi ◽

Xue Jiang ◽

Ke Li ◽

...

Keyword(s):

High Fat Diet ◽

Curcuma Longa ◽

Food Pellet ◽

Fasting Blood Glucose ◽

Density Lipoprotein ◽

Diet Group ◽

Lipoprotein Cholesterol ◽

Control Group ◽

Sprague Dawley ◽

High Fat

Curcumin is the main secondary metabolite of Curcuma longa and other Curcuma spp, and has been reported to have some potential in preventing and treating some physiological disorders. This study investigated the effect of curcumin in inhibiting high-fat diet and streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Twenty-six male Sprague-Dawley (SD) rats (170–190 g) were randomly divided into a standard food pellet diet group (Control group), a high-fat diet and streptozotocin group (HF + STZ group), and a high-fat diet combined with curcumin and STZ group (HF + Cur + STZ group). Compared with the HF + STZ group, the HF + Cur + STZ group exhibited significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (AST), and aspartate transaminase (ALT) levels, as well as liver coefficients. In the livers of these rats, the expression of malondialdehyde (MDA) and Bax was downregulated, whereas that of superoxide dismutase (SOD) and Bcl-2 was upregulated. Moreover, the liver histology of these rats was improved and resembled that of the control rats. These results suggest that curcumin prevents high-fat diet and STZ-induced hyperglycemia and hyperlipidemia, mainly via anti-oxidant and anti-apoptotic mechanisms in the liver.

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Antihiperlipidemi and Atheroprotective activity of Kepuh (Sterculia foetida) Leaves Ethanolic Extract on High-Fat-Diet Rat Models

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v3i1.4097 ◽

2017 ◽

Vol 3 (1) ◽

pp. 44

Author(s):

Ika Rahmawati Sutejo ◽

Imama Rasyada ◽

Annafira Yuniar

Keyword(s):

Cardiovascular Disease ◽

High Fat Diet ◽

Foam Cell ◽

Ethanolic Extract ◽

Foam Cells ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Aortic Tissue ◽

High Fat

Cardiovascular disease causes the highest mortality rate in the world. Atherosclerotic plaque is the most common etiology of cardiovascular disease. High levels of cholesterol in circulation is the pathophysiology of atherosclerosis formation. Atherosclerosis risk can be lowered to 20-40% with statins. Statins are effective in lowering total and LDL cholesterol, but these drugs have been reported to cause side effects. Therefore, it needed an alternative medicine to prevent the process of atherosclerosis using kepuh leaves (Sterculia foetida). Flavonoids contained in Kepuh leaves improve lipid profiles. The aims of this study is to prove the effectiveness of Sterculia foetida in lowering cholesterol, triglyceride and reduce the amount of foam cells on high-fat-diet induced rats. The research design is quasi-experimental with post test only design. Animals are grouped into normal group, negative control group and three treatment groups that were given kepuh leaves extract at the dose of 200 mg/kgBB, 400 mg/kgBB, and 800 mg/ kgBB. Rats were given high-fat diet of initial intravenous adrenaline injection 0.006 mg/200 gBB and duck egg yolks 5 g/kgBB for 21 days. Cholesterol and triglycerides were measured by enzymatic methods and aortic tissue stained with HE. ESf reduce cholesterol significantly and reduce the amount of foam cells on aorta.Keywords: cholesterol, triglyceride, kepuh leaves, foam cell, high-fat-diet

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Supplementation of Non-Dairy Creamer-Enriched High-Fat Diet with D-Allulose Ameliorated Blood Glucose and Body Fat Accumulation in C57BL/6J Mice

Applied Sciences ◽

10.3390/app9132750 ◽

2019 ◽

Vol 9 (13) ◽

pp. 2750 ◽

Cited By ~ 1

Author(s):

Ga Young Do ◽

Eun-Young Kwon ◽

Yun Jin Kim ◽

Youngji Han ◽

Seong-Bo Kim ◽

...

Keyword(s):

Adipose Tissue ◽

Lipid Metabolism ◽

Blood Glucose ◽

Body Fat ◽

High Fat Diet ◽

Fasting Blood Glucose ◽

Control Group ◽

High Fat ◽

Food Component ◽

Fat Accumulation

D-allulose, which has 70% of the sweet taste of sucrose but nearly no calories, has been reported to inhibit the absorption of lipids and suppress body weight gain in obese mice. Fats in non-dairy creamer consist of highly saturated fatty acids, which can cause various lipid disorders when consumed over a long period. We investigated whether D-allulose supplementation alleviates the effects of a non-dairy creamer-enriched high-fat diet on lipid metabolism. High-fat diets enriched with non-dairy creamer were administered to C57BL/6J mice with or without D-allulose supplementation for eight weeks by the pair-feeding design. Lipid metabolic markers were compared between the non-dairy creamer control group (NDC) and non-dairy creamer allulose group (NDCA). Body, adipose tissue, and liver weights, and fasting blood glucose levels, were significantly lower in the NDCA group than in the NDC group. Fecal fatty acid and triglyceride levels were significantly higher in the NDCA group than in the NDC group. Supplementing a non-dairy creamer-enriched high-fat diet with D-allulose improved overall lipid metabolism, including the plasma and hepatic lipid profiles, hepatic and adipose tissue morphology, and plasma inflammatory adipokine levels in mice. These results suggest that D-allulose can be used as a functional food component for preventing body fat accumulation from a high-fat diet that includes hydrogenated plant fats.

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Feeding Sprague Dawley Rats With Jordanian Wild Edible Plants and a High Fat Diet Reduced the Malondialdehyde Levels

Journal of Agricultural Science ◽

10.5539/jas.v11n10p71 ◽

2019 ◽

Vol 11 (10) ◽

pp. 71

Author(s):

Anwar A. Al-Assaff ◽

Hamed R. Takruri

Keyword(s):

High Fat Diet ◽

Fasting Blood Glucose ◽

Serum Levels ◽

The Other ◽

Control Group ◽

Sprague Dawley ◽

High Fat ◽

Treatment Groups ◽

Sprague Dawley Rats ◽

Plant Powders

The objective of this study was to determine the effect of selected Jordanian wild edible plant on lipid peroxidation and lipid profile in adult male Sprague Dawley rats fed high-fat diet. Fiftysix male, adult Sprague-Dawley rats at eight weeks of age, weighing about 200g were distributed into 7 experimental groups, 7 rats each . The groups included a negative control group that was fed a normal fat diet (NFD) and a possitve control group that was fed a high fat diet (HFD) (45% calories from fat). The six treatment groups were fed a HFD for the first 4 weeks of the experiment and a HFD with 9% of one of the selected dried plants for another 4 weeks. The treatment groups are sumac, thyme, clary, gundelia, garden rocket and wild mint. Blood samples were collected from the right heart ventricle. Serum malondialdehyde, lipid profile and fasting blood glucose were measured for rats. Results showed that the addition of different dried plant powders to the HFD did not significantly affect serum levels of TG, TC, HDL, LDL and fasting blood glucose. On the other hand, malondialdehyde (MDA) levels were significantly (p < 0.05) higher in the HFD group (4.09±0.45 mmol/ml) than those of other groups. MDA serum levels for the other groups were as follows: NFD (2.47±0.05), sumac (2.45±0.13), thyme (2.88±0.07), clary (2.97±0.16), garden rocket (2.96±0.11), gundelia (2.92±0.16) and wild mint (2.68±0.09). These levels were not sinificantly different from each other. It is concluded that incorporating dried plant powders in rat diets had a significantly positive effect only on lipid peroxidation assay as indicated by serum MDA levels.

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The Effect of Herbal Infusion on Fasting Blood Glucose (FBS) and Malondyaldehyde (MDA) Levels in Diabetic Rats

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1009.9 ◽

2020 ◽

Vol 1009 ◽

pp. 9-14

Author(s):

Apriliani Ismi Fauziah ◽

Lintang Dian Saraswati ◽

Ari Udiyono ◽

Beti Safitri

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Aloe Vera ◽

Control Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Diabetic Rats ◽

Control Group ◽

Albino Rats

Aloe vera, ginger, and sappan wood have recently gained attention as a potent antioxidant. This study was undertaken to analyze the possible effects of oral administration of herbal infusion (the combination of Aloe vera, ginger, and sapang wood) on fasting blood glucose (FBS) and malondialdehyde (MDA) levels in diabetic rats. Forty male albino rats (150-250 gm) were divided into five groups (n=8 in each group): alloxan-induced diabetic (negative control group/K-), acarbose treated (positive control group/K+), diabetic rats treated with herbal infusion dose 6.75 mg/150 gmbw (P1), diabetic rats treated with herbal infusion dose 13.5 mg/150 gmbw (P2), and diabetic rats treated with herbal infusion dose 20.25 mg/150 gmbw (P3). Male albino rats induced with a dose of alloxan 120 mg/kgbw and also 5% glucose after 6 hours of alloxan induction. FBS and MDA levels of each rat were measured before induction of alloxan was performed as a control sample. After seven days and 14 days of herbal infusion administration, FBS parameters was investigated with a glucometer. In the last week, the concentration of MDA in serum blood was determined using a UV-VIS spectrophotometer with a wavelength of 545 nm. Diabetic rats exhibited a significant decrease in FBS and MDA. Administration of herbal infusion was reduced FBS significantly between groups (ANOVA, Bonferroni, p<0.05). The concentration of MDA in serum blood was decreased significantly on K-, K+, P1, P2, P3 compared to the control group (ANOVA, LSD, P<0.05). It could be postulated herbal infusion has anti-hyperglycemic and anti-diabetic effects of FBS and MDA in male albino rats Wistar strain.

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Pemberian Ekstrak Buah Pare dan Diet Tinggi Lemak pada Penurunan Berat Badan Tikus Putih Jantan

Hang Tuah Medical journal ◽

10.30649/htmj.v17i1.185 ◽

2019 ◽

Vol 17 (1) ◽

pp. 60

Author(s):

I MADE SUBHAWA HARSA

Keyword(s):

Rattus Norvegicus ◽

High Fat Diet ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Momordica Charantia ◽

Control Group ◽

Bitter Melon ◽

High Fat ◽

White Rats

<div class="Section1">AbstractBackground : Obesity is an energy balance disorder, ketogenic diet can reduce body weight. Bitter melon (Momordica charantia L.) is a type of vegetable material that is often used as an antioxidant, hypocholesterolemia and hypotriglyceridemia drug. Cucurbitasin is a substance produced by bitter melon (Momordica charantia L.) with a bitter taste which causes in decreasing appetite, resulting in a decrease in triglycerides. This is caused by the bioactivity of bitter melon (Momordica charantia L.) which can increase lipid oxidation, which causes the accumulation of decreased fat tissue which can reduce weight.Methods: This research using pre and post test randomized controlled group design with sample size of 30 male white rats (Rattus norvegicus) wistar lines divided into 3 groups, group 1 as negative control group given standard feed, group 2 as positive control group given high-fat diet with a dose of 2.5 ml and group 3 as a treatment group given a high-fat feed with dose of 2.5 ml and extract of bitter melon (Momordica charantia L.) at dose of 37.5 mg / 150 gBB by feeding tube every morning for 28 days. Data were analyzed by T-paired tests.Result: The T-paired test in each group found a significantly different result of p = 0,000.Conclusion: given bitter melon (Momordica charantia L.) extract and high-fat diet can reduce weight in male white rats (Rattus norvegicus). Keywords:Bitter molon extract, high fat feed, weight loss. </div>

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The Anti-Atherogenic Activity of Luteolin and Luteolin-7-O-glucoside in High-Fat Diet-Administered Hyperhomocysteinemia-Induced C57BL/6J Mice

Current Developments in Nutrition ◽

10.1093/cdn/nzaa045_109 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 476-476

Author(s):

Youngsun Song ◽

Chung-Mu Park

Keyword(s):

Protein Expression ◽

Adhesion Molecules ◽

High Fat Diet ◽

Inflammatory Markers ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

High Fat ◽

Funding Sources ◽

Tnf Α

Abstract Objectives The anti-atherogenic activity of luteolin and one of its glycoside form, luteolin-7-O-glucoside, was compared in high fat fed homocysteinemia-induced C57BL/6J mice. Methods Forty male C57BL/6J mice (12 wks) were divided into 4 groups; negative control group, homocystein (Hcy) control group, luteolin and luteolin-7-O-glucoside groups and fed by modified AIN-93 with 45% fat and 0.5% cholesterol. Flavones were administered by oral gavage (50 mg/Kg BW) and Hcy (0.9 g/L) were provided in drinking water for 6 weeks. Results The serum concnetrations of Hcy, TG, cholesterol, TNF-α and MCP-1 were significantly increased in Hcy control group, which were mitigated in luteolin and luteolin-7-O-glycoside groups (P < 0.05). Protein expression levels of adhesion molecules, inflammatory markers, such as VCAM, ICAM and COX-2 in liver and aorta were attenuated in luteolin and luteolin-7-O-glycoside groups compared to Hcy control group (P < 0.05). Histopathologic examination by H&E staining and immunohistochemistry in liver and aorta was compatible with protein expression data. Conclusions Luteolin and luteolin-7–O-glycoside exhibited anti-atherogenic activity through the regulation of inflammatory markers and adhesion molecules in high fat-fed hyperhomocysteinemia induced mice. Efficacy of luteolun and luteolin-7-O-glycoside was not different. Funding Sources This work was supported by the National Research Foundation of Korea (NRF) grant by the Korea government of MOE (No 201,704,340,001).

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Gambaran Glukosa Darah Setelah Latihan Fisik pada Tikus Wistar Diabetes Melitus yang Diinduksi Aloksan

Jurnal Kesehatan Andalas ◽

10.25077/jka.v4i1.179 ◽

2015 ◽

Vol 4 (1) ◽

Author(s):

Ahmad Syukri Harahap ◽

Rahmatina B. Herman ◽

Eti Yerizel

Keyword(s):

Blood Glucose ◽

Physical Exercise ◽

Fasting Blood Glucose ◽

Negative Control Group ◽

Treated Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Diabetes Melitus

AbstrakDiabetes Melitus telah dikategorikan sebagai penyakit global yang prevalensinya terus meningkat dan penyebab utama morbiditas dan mortalitas. Latihan fisik merupakan salah satu tatalaksana untuk mengontrol glukosa darah secara nonfarmakologis. Tujuan dari penelitian ini adalah untuk mengetahui gambaran kadar glukosa darah pada tikus Wistar diabetes melitus yang diinduksi aloksan setelah pemberian latihan fisik. Penelitian eksperimental ini menggunakan 18 ekor tikus Wistar dengan berat badan 150-200 gram yang dibagi menjadi tiga kelompok, yaitu 6 ekor kelompok kontrol negatif (K-), 6 ekor kelompok kontrol positif (K+) dengan induksi aloksan tanpa pemberian latihan fisik dan 6 ekor kelompok perlakuan (P) dengan induksi aloksan dan diberi latihan fisik selama 4 minggu. Pengukuran kadar glukosa darah dilakukan dengan glucometer merek Accu-Check. Hasil penelitian menunjukkan peningkatan rata-rata kadar glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan perlakuan (P) setelah periode induksi yaitu, pada kelompok kontrol negatif 84,83±6,88 mg/dl, kelompok kontrol positif 220,80±12,29 mg/dl, dan kelompok perlakuan 248,50±94,55 mg/dl (p<0,05). Setelah periode latihan fisik, terdapat penurunan rata-rata glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan Perlakuan (P), namun penurunan pada kelompok perlakuan (P) lebih besar secara bermakna dibandingkan dengan kelompok kontrol positif (K+), yaitu rata-rata glukosa darah puasa kelompok kontrol positif 192,00±12,00 mg/dl dan kelompok perlakuan 163,00±20,26 mg/dl (p<0,05). Kesimpulan hasil penelitian ini adalah terdapat penurunan kadar glukosa darah puasa setelah latihan fisik.Kata kunci: latihan fisik, glukosa darah, diabetes melitusAbstractDiabetes Melitus has been categorized as a global disease which have increasing prevalence and the mine cause of morbidity and mortality. Phyisical exercise is one of the nonpharmacological treatment to control blood glucose. The objective of this research was to determine the blood gucose levels of aloksan induced’s wistar rat after physical exercise.The research used 18 wistar rats 150-200 gram weight, divided into three groups, as six negative control group (K-), six positive control group inducing aloksan without physical exercise (K+) and six treated group inducing aloksan with physical exercise for four weeks (P). Blood glucose was measured by Accu Check’s glucometer.The result showed an increase in fasting blood glucose level significantly on positive control group (K-) and treated group (P) after induced period, which was 84,83±6,88 mg/dl on negative control group, 220,80±12,29 mg/dl on positive control group, and 248,50±94,55 mg/dl on treated group (p<0,05). After physical exercise period, there was a decrease in fasting blood significantly on K+ and P, but in P group have more greater than K+ group, 192,00±12,00 mg/dl on positive control group (K+) and 163,00±20,26 mg/dl on treated group (P).Conclusion of this research is a decrease in fasting blood glucose levels after phyisical exercise.Keywords: physical exercise, blood glucose, diabetes melitus

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Ekstrak Kulit Pisang Kepok (Musa paradisiaca) Menurunkan Kadar AST pada Tikus Putih yang Diinduksi Diet Tinggi Lemak

Hang Tuah Medical journal ◽

10.30649/htmj.v18i2.513 ◽

2021 ◽

Vol 18 (2) ◽

pp. 172

Author(s):

STEFANUS DJONI HUSODO ◽

INDRI NGESTI RAHAYU ◽

ASAMI RIETTA KUMALA

Keyword(s):

High Fat Diet ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Banana Peel ◽

Control Group ◽

High Fat ◽

Musa Paradisiaca ◽

Peel Extract ◽

K2 Group

Background. Non-alcoholic fatty liver disease (NAFLD) causes many health problems in overweight person. There is an increase in the incidence of NAFLD with the increased incidence of metabolic syndrome. NAFLD is closely related to the incidence of obesity and dyslipidemia. Increased levels of AST and ALT are the most frequently performed liver function tests that can be used as a benchmark for suspicion of NAFLD. Banana peels contain flavonoids and phenolics which are antioxidants that can function as hepatoprotectors.Aim. Proving the effect of Kepok banana peel extract (Musa paradisiaca formatypica) on AST and ALT of Wistar rats induced high fat diet.Method. A laboratory experimental study with a post-test only control group design, with four treatment groups. There is one negative control group only given four weeks standard diet, one positive control group only given four weeks high-fat diet, and two treatment group where the rats were given two weeks high-fat diet then were given two weeks high-fat diet plus Kepok banana peel extract at a dose of 200 mg/kg BW and 400 mg/kg BW.Result. Based on the results of descriptive analysis, the highest mean AST levels were in the negative control group and the lowest was the K2 group. Meanwhile, the highest mean of ALT was in the positive control group and the lowest was the K2 group. Based on the Kruskal Wallis test, the significance of the AST variable was not different (p>0.05), but the ALT variable was significantly different (p<0.05). Significant differences from the Mann Whitney-U test (p<0.05) were obtained between the negative control group and the positive control, the negative control group with K2 and the positive control group with the K2 group).Conclusion. The administration of Kepok banana peel extract (Musa paradisiaca formatypica) showed a significant decrease in ALT levels in Wistar strain white rats induced by a high-fat diet, but it was not significant for AST levels.

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