scholarly journals The Anti-Atherogenic Activity of Luteolin and Luteolin-7-O-glucoside in High-Fat Diet-Administered Hyperhomocysteinemia-Induced C57BL/6J Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 476-476
Author(s):  
Youngsun Song ◽  
Chung-Mu Park
Keyword(s):  
Protein Expression ◽  
Adhesion Molecules ◽  
High Fat Diet ◽  
Inflammatory Markers ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
High Fat ◽  
Funding Sources ◽  
Tnf Α

Abstract Objectives The anti-atherogenic activity of luteolin and one of its glycoside form, luteolin-7-O-glucoside, was compared in high fat fed homocysteinemia-induced C57BL/6J mice. Methods Forty male C57BL/6J mice (12 wks) were divided into 4 groups; negative control group, homocystein (Hcy) control group, luteolin and luteolin-7-O-glucoside groups and fed by modified AIN-93 with 45% fat and 0.5% cholesterol. Flavones were administered by oral gavage (50 mg/Kg BW) and Hcy (0.9 g/L) were provided in drinking water for 6 weeks. Results The serum concnetrations of Hcy, TG, cholesterol, TNF-α and MCP-1 were significantly increased in Hcy control group, which were mitigated in luteolin and luteolin-7-O-glycoside groups (P < 0.05). Protein expression levels of adhesion molecules, inflammatory markers, such as VCAM, ICAM and COX-2 in liver and aorta were attenuated in luteolin and luteolin-7-O-glycoside groups compared to Hcy control group (P < 0.05). Histopathologic examination by H&E staining and immunohistochemistry in liver and aorta was compatible with protein expression data. Conclusions Luteolin and luteolin-7–O-glycoside exhibited anti-atherogenic activity through the regulation of inflammatory markers and adhesion molecules in high fat-fed hyperhomocysteinemia induced mice. Efficacy of luteolun and luteolin-7-O-glycoside was not different. Funding Sources This work was supported by the National Research Foundation of Korea (NRF) grant by the Korea government of MOE (No 201,704,340,001).

scholarly journals Antihiperlipidemi and Atheroprotective activity of Kepuh (Sterculia foetida) Leaves Ethanolic Extract on High-Fat-Diet Rat Models

2017 ◽  
Vol 3 (1) ◽  
pp. 44
Author(s):  
Ika Rahmawati Sutejo ◽  
Imama Rasyada ◽  
Annafira Yuniar
Keyword(s):  
Cardiovascular Disease ◽  
High Fat Diet ◽  
Foam Cell ◽  
Ethanolic Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aortic Tissue ◽  
High Fat

Cardiovascular disease causes the highest mortality rate in the world. Atherosclerotic plaque is the most common etiology of cardiovascular disease. High levels of cholesterol in circulation is the pathophysiology of atherosclerosis formation. Atherosclerosis risk can be lowered to 20-40% with statins. Statins are effective in lowering total and LDL cholesterol, but these drugs have been reported to cause side effects. Therefore, it needed an alternative medicine to prevent the process of atherosclerosis using kepuh leaves (Sterculia foetida). Flavonoids contained in Kepuh leaves improve lipid profiles. The aims of this study is to prove the effectiveness of Sterculia foetida in lowering cholesterol, triglyceride and reduce the amount of foam cells on high-fat-diet induced rats. The research design is quasi-experimental with post test only design. Animals are grouped into normal group, negative control group and three treatment groups that were given kepuh leaves extract at the dose of 200 mg/kgBB, 400 mg/kgBB, and 800 mg/ kgBB. Rats were given high-fat diet of initial intravenous adrenaline injection 0.006 mg/200 gBB and duck egg yolks 5 g/kgBB for 21 days. Cholesterol and triglycerides were measured by enzymatic methods and aortic tissue stained with HE. ESf reduce cholesterol significantly and reduce the amount of foam cells on aorta.Keywords: cholesterol, triglyceride, kepuh leaves, foam cell, high-fat-diet

Effect of Administration of CHAGURO Made of Chayote (Sechium edule) and Tuna (Thunnus sp.) on Rats Induced with Streptozotocin-Nicotinamide and a High-Fat Diet

2021 ◽  
Vol 9 (1) ◽  
pp. 258-266
Author(s):  
Toto Sudargo ◽  
Bianda Aulia ◽  
Atika Anif Prameswari ◽  
Alim Isnansetyo ◽  
Indun Dewi Puspita ◽  
...  
Keyword(s):  
Blood Glucose ◽  
High Fat Diet ◽  
Fasting Blood Glucose ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Tuna Fish ◽  
Sechium Edule

This study was conducted to develop Chaguro, a low-cost supplementary food made of chayote (Sechium edule (Jacq.) Swartz) and tuna fish (Thunnus sp.), for diabetes and dyslipidemia diet therapy. In order to find a formula with effective hypoglycaemic and antidyslipidemic properties, dried tuna and chayote were mixed at different ratios: F1 (75% tuna, 25% chayote), F2 (50% tuna, 50% chayote), and F3 (25% tuna, 75% chayote). Thirty male Sprague Dawley rats were assigned into healthy control group or groups induced with streptozotocin-nicotinamide and a high-fat diet. Chaguro was administered 2.7 g/ kgBW/ day using a gavage for 28 days. The administration of all Chaguro formulas improved blood markers compared to the negative control group (p < 0.001). Chaguro F2 lowered fasting blood glucose (97.07±1.18 vs 266.31±5.31), total cholesterol (113.59±2.22 vs 208.78±4.31), triglycerides (89.93±2.51 vs 142.35±2.83), LDL-c (33.87±1.87 vs 87.85±3.34) and increased HDL-c (69,08±1,85 vs 23,91±1,64) level the most compared to the negative control group (p < 0.001). Streptozotocin-induced weight loss was also prevented in all diabetic rats fed with Chaguro, with the bodyweight being similar to that of healthy controls at the end of the intervention (p < 0.001). This study found that Chaguro may be a potential food product to help lower blood glucose and improve lipid profile in diabetes and dyslipidemia.

scholarly journals Pemberian Ekstrak Buah Pare dan Diet Tinggi Lemak pada Penurunan Berat Badan Tikus Putih Jantan

2019 ◽  
Vol 17 (1) ◽  
pp. 60
Author(s):  
I MADE SUBHAWA HARSA
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Control Group ◽  
Bitter Melon ◽  
High Fat ◽  
White Rats

<div class="Section1"><p align="center"><strong>Abstra</strong><strong>ct</strong></p><p><strong>Background</strong> : Obesity is an energy balance disorder, ketogenic diet can reduce body weight. Bitter melon (<em>Momordica charantia L.)</em> is a type of vegetable material that is often used as an antioxidant, hypocholesterolemia and hypotriglyceridemia drug. <em>Cucurbitasin</em> is a substance produced by bitter melon (<em>Momordica charantia L.)</em> with a bitter taste which causes in decreasing appetite, resulting in a decrease in triglycerides. This is caused by the bioactivity of bitter melon (<em>Momordica charantia L.)</em> which can increase lipid oxidation, which causes the accumulation of decreased fat tissue which can reduce weight.</p><p><strong>Methods</strong>: This research using pre and post test randomized controlled group design with sample size of 30 male white rats (<em>Rattus norvegicus</em>) wistar lines divided into 3 groups, group 1 as negative control group given standard feed, group 2 as positive control group given high-fat diet with a dose of 2.5 ml and group 3 as a treatment group given a high-fat feed with dose of 2.5 ml and extract of bitter melon (<em>Momordica charantia L.)</em> at dose of 37.5 mg / 150 gBB by feeding tube every morning for 28 days. Data were analyzed by T-paired tests.</p><p><strong>Result</strong>: The T-paired test in each group found a significantly different result of p = 0,000.</p><p><strong>Conclusion</strong>: given bitter melon (<em>Momordica charantia L.)</em> extract and high-fat diet can reduce weight in male white rats (<em>Rattus norvegicus</em>).</p><p> </p><p><strong>Keywords</strong>:Bitter molon extract, high fat feed, weight loss.</p><p> </p></div><strong><br clear="all" /></strong>

scholarly journals Ekstrak Kulit Pisang Kepok (Musa paradisiaca) Menurunkan Kadar AST pada Tikus Putih yang Diinduksi Diet Tinggi Lemak

2021 ◽  
Vol 18 (2) ◽  
pp. 172
Author(s):  
STEFANUS DJONI HUSODO ◽  
INDRI NGESTI RAHAYU ◽  
ASAMI RIETTA KUMALA
Keyword(s):  
High Fat Diet ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Banana Peel ◽  
Control Group ◽  
High Fat ◽  
Musa Paradisiaca ◽  
Peel Extract ◽  
K2 Group

<p class="ABSTRACT">Background. Non-alcoholic fatty liver disease (NAFLD) causes many health problems in overweight person. There is an increase in the incidence of NAFLD with the increased incidence of metabolic syndrome. NAFLD is closely related to the incidence of obesity and dyslipidemia. Increased levels of AST and ALT are the most frequently performed liver function tests that can be used as a benchmark for suspicion of NAFLD. Banana peels contain flavonoids and phenolics which are antioxidants that can function as hepatoprotectors.</p><p class="ABSTRACT">Aim. Proving the effect of Kepok banana peel extract (Musa paradisiaca formatypica) on AST and ALT of Wistar rats induced high fat diet.</p><p class="ABSTRACT">Method. A laboratory experimental study with a post-test only control group design, with four treatment groups. There is one negative control group only given four weeks standard diet, one positive control group only given four weeks high-fat diet, and two treatment group where the rats were given two weeks high-fat diet then were given two weeks high-fat diet plus Kepok banana peel extract at a dose of 200 mg/kg BW and 400 mg/kg BW.</p><p class="ABSTRACT">Result. Based on the results of descriptive analysis, the highest mean AST levels were in the negative control group and the lowest was the K2 group. Meanwhile, the highest mean of ALT was in the positive control group and the lowest was the K2 group. Based on the Kruskal Wallis test, the significance of the AST variable was not different (p&gt;0.05), but the ALT variable was significantly different (p&lt;0.05). Significant differences from the Mann Whitney-U test (p&lt;0.05) were obtained between the negative control group and the positive control, the negative control group with K2 and the positive control group with the K2 group).</p><p class="ABSTRACT">Conclusion. The administration of Kepok banana peel extract (Musa paradisiaca formatypica) showed a significant decrease in ALT levels in Wistar strain white rats induced by a high-fat diet, but it was not significant for AST levels.</p>

scholarly journals BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 225
Author(s):  
Sulaeman A ◽  
Patonah Patonah ◽  
Patonah Patonah ◽  
Negara Gg ◽  
Negara Gg
Keyword(s):  
Test Group ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Adipose Tissues ◽  
Obese Male

  Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

scholarly journals Therapeutic effect of various ginsenosides on rheumatoid arthritis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Meng Zhang ◽  
Hongwei Ren ◽  
Kun Li ◽  
Shengsheng Xie ◽  
Ru Zhang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Flow Cytometry ◽  
T Cell ◽  
Therapeutic Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Potential Candidate ◽  
Tnf Α ◽  
Huvec Cells

Abstract Background Rheumatoid arthritis (RA) is an autoimmune disease which causes disability and threatens the health of humans. Therefore, it is of great significance to seek novel effective drugs for RA. It has been reported that various ginsenoside monomers are able to treat RA. However, it is still unclear which ginsenoside is the most effective and has the potential to be developed into an anti-RA drug. Methods The ginsenosides, including Rg1, Rg3, Rg5, Rb1, Rh2 and CK, were evaluated and compared for their therapeutic effect on RA. In in vitro cell studies, methotrexate (MTX) and 0.05% dimethyl sulfoxide (DMSO) was set as a positive control group and a negative control group, respectively. LPS-induced RAW264.7 cells and TNF-α-induced HUVEC cells were cultured with MTX, DMSO and six ginsenosides, respectively. Cell proliferation was analyzed by MTT assay and cell apoptosis was carried out by flow cytometry. CIA mice model was developed to evaluate the therapeutic efficacy of ginsenosides. The analysis of histology, immunohistochemistry, flow cytometry and cytokine detections of the joint tissues were performed to elucidate the action mechanisms of ginsenosides. Results All six ginsenosides showed good therapeutic effect on acute arthritis compared with the negative control group, Ginsenoside CK provided the most effective treatment ability. It could significantly inhibit the proliferation and promote the apoptosis of RAW 264.7 and HUVEC cells, and substantially reduce the swelling, redness, functional impairment of joints and the pathological changes of CIA mice. Meanwhile, CK could increase CD8 + T cell to down-regulate the immune response, decrease the number of activated CD4 + T cell and proinflammatory M1-macrophages, thus resulting in the inhibition of the secretion of proinflammatory cytokine such as TNF-α and IL-6. Conclusion Ginsenoside CK was proved to be a most potential candidate among the tested ginsenosides for the treatment of RA, with a strong anti-inflammation and immune modulating capabilities.

scholarly journals PENGARUH PEMBERIAN JUS BUAH BELIMBING WULUH (AVERRHOA BILIMBI L.) TERHADAP KADAR KOLESTEROL LDL DARAH TIKUS PUTIH (RATTUS NORVEGICUS) JANTAN GALUR WISTAR DENGAN DIET TINGGI LEMAK

2019 ◽  
Vol 8 (2) ◽  
pp. 35-41
Author(s):  
Kartika Dwi Rahminiwati ◽  
◽  
IGM Antara Hambarsika ◽  
Fitri Handajani ◽  
◽  
...  
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
High Fat ◽  
Cholesterol Levels ◽  
The Mean

A high-fat diet can increase lipoprotein levels, total cholesterol, (Low Density Lipoprotein) LDL, and triglycerides. Starfruit has saponin and flavonoid compounds which are expected to reducing LDL cholesterol levels. The aim of the study was to determine the effect of starfruit juice on lowering the blood cholesterol LDL of Wistar rats fed with high-fat diet. Experimental study with post-test only control group design. As many as 24 male white rats from the Wistar strain were divided into 3 groups: negative control groups (K-) that were given standard feed for 28 days; positive control group (K+) who were given a high-fat diet for 28 days; the treatment group (KP) was given a high-fat diet for 28 days and on the 15th day 28th they were given a starfruit juice with a dose of 4ml / 200grBB / day. Day 29 measured LDL cholesterol. The statistic test showed a significant increase in LDL cholesterol levels (p=0.001) in the positive control group (x=12.125±2.642 mg/dL) compared to the negative control group (x=7.625±1.506 mg/dL). There was no significant different the mean cholesterol level of the treatment group (x=11±1.927) compared to the mean LDL cholesterol level of the positive control group (x=12.125±2.642). A high-fat diet significantly increases the mean LDL cholesterol level. Starfruit juice did not significantly reduce LDL cholesterol level.

scholarly journals Regulation of Inflammatory Response in Human Osteoarthritic Chondrocytes by Novel Herbal Small Molecules

2019 ◽  
Vol 20 (22) ◽  
pp. 5745 ◽  
Author(s):  
Ziadlou ◽  
Barbero ◽  
Stoddart ◽  
Wirth ◽  
Li ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Catabolic Genes ◽  
Osteoarthritic Chondrocytes ◽  
Culture Model ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Safranin O ◽  
Vanilic Acid

In this study, 34 Traditional Chinese Medicine (TCM) compounds were screened for potential anabolic and anti-inflammatory properties on human osteoarthritic (OA) chondrocytes. The anabolic effects were assessed by measuring the glycosaminoglycan (GAG) relative to the DNA content using a 3D pellet culture model. The most chondrogenic compounds were tested in an inflammatory model consisting of 3 days of treatment with cytokines (IL-1β/TNF-α) with or without supplementation of TCM compounds. The anti-inflammatory effects were assessed transcriptionally, biochemically and histologically. From the 34 compounds, Vanilic acid (VA), Epimedin A (Epi A) and C (Epi C), 2′′-O-rhamnosylicariside II (2-O-rhs II), Icariin, Psoralidin (PS), Protocatechuicaldehyde (PCA), 4-Hydroxybenzoic acid (4-HBA) and 5-Hydroxymethylfurfural (5-HMF) showed the most profound anabolic effects. After induction of inflammation, pro-inflammatory and catabolic genes were upregulated, and GAG/DNA was decreased. VA, Epi C, PS, PCA, 4-HBA and 5-HMF exhibited anti-catabolic and anti-inflammatory effects and prevented the up-regulation of pro-inflammatory markers including metalloproteinases and cyclooxygenase 2. After two weeks of treatment with TCM compounds, the GAG/DNA ratio was restored compared with the negative control group. Immunohistochemistry and Safranin-O staining confirmed superior amounts of cartilaginous matrix in treated pellets. In conclusion, VA, Epi C, PS, PCA, 4-HBA and 5-HMF showed promising anabolic and anti-inflammatory effects.

scholarly journals Inhibitory effect of baicalin on orthodontically induced inflammatory root resorption in rats

2020 ◽  
Vol 48 (9) ◽  
pp. 030006052095507
Author(s):  
Peng Lin ◽  
Xin-xing Guo ◽  
Yi-ling Wang ◽  
Zhu-liang Wei ◽  
Hai-yan Xin ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Negative Control Group ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Area Ratio ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Movement Distance

Objective This study investigated the inhibitory effect of baicalin on orthodontically induced inflammatory root resorption in rats. Methods Forty-five male Wistar rats were randomly divided into three groups of 15 rats each. Fifty grams of force was used to establish an orthodontic tooth movement model. Baicalin (40 mg/kg) was locally injected into rats in the baicalin group at 3-day intervals; concurrently, normal saline was injected into rats in the negative control group. On the 21st day after orthodontic treatment, the tooth movement distance and root resorption area ratio were measured. Histomorphology changes were observed by hematoxylin and eosin staining and immunohistochemistry. Results There was no significant difference in tooth movement distance between groups. The root resorption area ratio was significantly lower in the baicalin group than in the negative control group. Runx-2 expression was significantly higher in the baicalin group than in the negative control group, while tumor necrosis factor (TNF)-α expression was significantly lower in the baicalin group than in the negative control group. Conclusions Baicalin inhibits orthodontically induced inflammatory root resorption by enhancing the expression of Runx-2 and reducing the expression of TNF-α, but does not affect tooth movement distance.

Effect of dietary curcumin and capsaicin on testicular and hepatic oxidant–antioxidant status in rats fed a high-fat diet

2019 ◽  
Vol 44 (7) ◽  
pp. 774-782 ◽  
Author(s):  
Sevda Tanrıkulu-Küçük ◽  
Canan Başaran-Küçükgergin ◽  
Muhammed Seyithanoğlu ◽  
Semra Doğru-Abbasoğlu ◽  
Hikmet Koçak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
High Fat Diet ◽  
Antioxidant Status ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Transferase Activity ◽  
High Fat

This study investigated the effects of curcumin and capsaicin on testicular and hepatic oxidant–antioxidant status in rats fed a high-fat diet (HFD). Male Sprague–Dawley rats were divided into 5 groups (8 rats per group). The control group was fed a normal control diet (standard laboratory chow), the HFD group was fed HFD (60% of total calories from fat), the HFD+CUR group received HFD supplemented with curcumin (1.5 g curcumin/kg HFD), the HFD+CAP group was given HFD supplemented with capsaicin (0.15 g capsaicin/kg HFD), and the HFD+CUR+CAP group received HFD supplemented with curcumin and capsaicin for 16 weeks. Hepatic and testicular thiobarbituric acid reactive substances (TBARS), reactive oxygen species (ROS), glutathione (GSH) levels, glutathione transferase activity, and Cu-Zn superoxide dismutase, glutathione peroxidase, and catalase protein expression and enzyme activities were measured. Protein expression was determined by Western blotting. GSH levels and antioxidant enzyme activities were measured with colorimetric methods. HFD slightly increased hepatic and testicular oxidative stress parameters. GSH levels did not change between groups. TBARS and ROS levels were significantly reduced in the HFD+CUR+CAP group compared with the HFD group. Liver and testis antioxidant enzyme activities and expression increased significantly with combined capsaicin and curcumin treatment. Curcumin and capsaicin treatment attenuated testicular and hepatic oxidative stress and enhanced the antioxidant defense system. The combination of capsaicin and curcumin with HFD seems to have some remarkable and beneficial effects on testicular oxidative damage in the fatty liver rat model.

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