scholarly journals Effective methods for increasing coumestrol in soybean sprouts

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260147
Author(s):  
Tomoe Ohta ◽  
Takuhiro Uto ◽  
Hiromitsu Tanaka
Keyword(s):  
High Performance ◽  
Health Benefits ◽  
Biologically Active ◽  
Analysis Method ◽  
Light Conditions ◽  
Soybean Sprouts ◽  
Water Conditions ◽  
Quantitative Analysis Method ◽  
The Difference ◽  
Growth Of Fungi

Coumestrol (CM), a biologically active compound found in Leguminosae plants, provides various human health benefits. To identify easy and effective methods to increase CM content in vegetables, we developed a quantitative analysis method using high-performance liquid chromatography (HPLC). Using this method, we found that soybean sprouts (1.76 ± 0.13 μg/g) have high CM contents among nine vegetables and evaluated the difference in CM contents between two organs of the sprouts: cotyledons and hypocotyls. Next, soybean sprouts were cultivated under different light, temperature, and water conditions and their CM contents were evaluated. CM content was higher in hypocotyls (4.11 ± 0.04 μg/g) than in cotyledons. Cultivating soybean sprouts at 24°C enhanced CM content regardless of light conditions, the growth of fungi and bacteria, and sprout color. Thus, we identified methods of soybean sprout cultivation to increase CM content, which may provide health benefits and enhance value.

A HPTLC Densitometer Determination of Sinapic acid in Chandrasur (Lepidium sativum)

2008 ◽  
Vol 1 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Preeti Sagar Nayak ◽  
Sunil D Upadhyaya ◽  
Anubha Upadhyaya
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
High Performance ◽  
Sinapic Acid ◽  
Lepidium Sativum ◽  
Analysis Method ◽  
Qualitative And Quantitative Analysis ◽  
Quantitative Analysis Method ◽  
Acetic Acid Water

A qualitative and quantitative analysis method was established to improve quality assessment standards for Lepidium sativum seeds. The sinapic acid was identified by comparing with standards and quantified simultaneously by High performance thin layer chromatographic (HPTLC). HPTLC of Lepidium sativum methanolic extract was performed on Silica gel 60F254 [20 cm × 10 cm] plates with butanol: acetic acid: water (4:1:5), as mobile phase. Quantitative evaluation of the plate was performed in the absorbance-reflectance mode at 326 nm. The sinapic acid was separated on a thin layer of silica gel and determined by HPTLC-photo densitometry. The proposed method is simple, sensitive and can be used for the routine assay. Keywords: Lepidium sativum; Sinapic acid; HPTLC; Photo densitometry. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1196  

scholarly journals Development and Validation of an Analytical Method for Deacetylasperulosidic Acid, Asperulosidic Acid, Scopolin, Asperuloside and Scopoletin in Fermented Morinda citrifolia L. (Noni)

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 80
Author(s):  
Sun-Il Choi ◽  
Hee-Yeon Kwon ◽  
Im-Joung La ◽  
Yeon-Hui Jo ◽  
Xionggao Han ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Biologically Active ◽  
Morinda Citrifolia ◽  
Coefficient Of Determination ◽  
Analysis Method ◽  
Food Ingredients

Fermentation is a technology that enhances biologically active ingredients, improves the absorption rate and induces the generation of new functional ingredients by the catalytic action of enzyme systems possessed by microorganisms. In this study, changes in the content of five kinds of bioactive compounds (deacetylasperulosidic acid, asperulosidic acid, scopolin, asperuloside and scopoletin) of Morinda citrifolia L. were confirmed by fermentation, and a high-performance liquid chromatography-photodiode array (HPLC-PDA) analysis method for measuring analytes was developed and validated. HPLC method for the determination of five bioactive compounds in Morinda citrifolia L. extracts (MCE) was validated in terms of sensitivity, linearity, selectivity, limit of detection (LOD) and quantification (LOQ), precision and accuracy. The coefficient of determination of the calibration curve for bioactive compounds (1.56–100 μg/mL) showed linearity (R2 ≥ 0.9999). LOD and LOQ were in the range 0.04–0.97 and 0.13–2.95 μg/mL, respectively. The range of intra- and intraday accuracies values (recovery) were 97.5–121.9% and 98.8–118.1%, respectively, and precision value (RSDs) of the bioactive compounds were <4%. In addition, changes in the content of five bioactive compounds in MCE by fermentation were confirmed. These results indicate that the developed fermentation and analysis method could be applied in the development of potential functional food ingredients.

Implementasi Sistem Informasi Monitoring Pengolahan Data Inventory Gudang Pada PT.Talaga Mulya Indah

2020 ◽  
Vol 6 (2) ◽  
pp. 187-197
Author(s):  
Nurlaila Suci Rahayu Rais ◽  
Dedeh Apriyani ◽  
Gito Gardjito
Keyword(s):  
Data Processing ◽  
Integrated Control ◽  
Unified Modeling Language ◽  
Review Literature ◽  
Analysis Method ◽  
Inventory Data ◽  
Literature Study ◽  
Unified Modeling ◽  
The Difference ◽  
The Right

Monitoring of warehouse inventory data processing is an important thing for companies. PT Talaga mulya indah is still manual using paper media, causing problems that have an effect on existing information, namely: problems with data processing of incoming and outgoing goods. And the difference between data on the amount of stock of goods available with physical data, often occurs inputting data more than once for the same item, searching for available data, and making reports so that it impedes companies in monitoring inventory of existing stock of goods. Which aims to create a system that can provide updated information to facilitate the warehouse admin in making inventory reports, and reduce errors in input by means of integrated control. In this study, the authors used the data collection method used in this analysis using the method of observation, interviews, and literature review (literature study). For analysis using the PIECES analysis method. Furthermore, the system design used is UML (Unified Modeling Language). The results of this study are expected to produce the right data in the process of monitoring inventory data processing, also can provide the right information and make it easier to control the overall availability of goods.

Pandhawa Gubah sebagai Representasi Interaksi Metafisik Manusia Jawa dan Perbandingannya dengan Cheritera Pandawa Lima

Manuskripta ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Novarina Novarina
Keyword(s):  
Comparative Study ◽  
Literary Theory ◽  
Research Method ◽  
Descriptive Analysis ◽  
Literary Study ◽  
Analysis Method ◽  
Research Objects ◽  
Similarities And Differences ◽  
The Difference ◽  
The Comparative Study

Abstract: This research is a comparative literary study that uses Malay and Javanese versions of Mahabarata text sources. The research objects used were the text edition of Pandhawa Gubah (PG) by Sudibjo Z. Hadisutjipto and the text of Cheritera Pandawa Lima (CPL) by Khalid Hussain. The research method used is descriptive-analysis method. In the comparative study used a comparative literary theory proposed by Endraswara (2011). The results of the text comparison reveal the similarities and differences in the image of Bima figures in the Javanese and Malay versions. The equation as a whole is that both texts contain the same heroic storyline and heroic character, Bima. In addition, Indian influence is still evident in the two texts seen from the nuances of Hinduism that exist in both texts. While the difference is seen in the events that accompany Bima's struggle in achieving his victory. Based on these similarities and differences, it can be seen that the authors attempt to represent the concept of metaphysical interactions vertically and horizontally expressed through PG text. --- Abstrak: Penelitian ini adalah satu kajian sastra bandingan yang menggunakan sumber teks Mahabarata versi Melayu dan Jawa. Objek penelitian yang digunakan adalah edisi teks Pandhawa Gubah (PG) karya Sudibjo Z. Hadisutjipto dan teks Cheritera Pandawa Lima (CPL) karya Khalid Hussain. Metode penelitian yang digunakan adalah metode deskriptif-analisis. Dalam telaah perbandingan digunakan teori sastra bandingan yang dikemukakan Endraswara (2011). Hasil perbandingan teks mengungkapkan adanya persamaan dan perbedaan citra tokoh Bima dalam versi Jawa maupun versi Melayu. Persamaan secara keseluruhan adalah kedua teks tersebut mengandung alur cerita kepahlawanan dan tokoh pahlawan yang sama yaitu Bima. Selain itu, pengaruh India masih tampak dalam kedua teks tersebut dilihat dari nuansa Hinduisme yang ada dalam kedua teks. Sementara perbedaannya tampak pada peristiwa-peristiwa yang menyertai perjuangan Bima dalam mencapai kemenangannya. Berdasarkan persamaan dan perbedaan tersebut tampak adanya upaya penulis untuk merepresentasikan konsep interaksi metafisik secara vertikal dan horizontal yang diungkapkan melalui teks PG.

Kesalahan Penggunaan Bahasa Perancis dalam Tulisan Mahasiswa Program Studi Sastra Perancis Unpad

Metahumaniora ◽  
2017 ◽  
Vol 7 (3) ◽  
pp. 368
Author(s):  
Gilang Januarsyah
Keyword(s):  
General Public ◽  
Qualitative Approach ◽  
French Language ◽  
Analysis Method ◽  
Writing Skill ◽  
Language Writing ◽  
The Difference ◽  
Observation Method ◽  
Bahasa Indonesia

ABSTRAKUntuk menghasilkan sebuah karangan berbahasa Perancis yang dapat dipahamioleh khalayak umum, mahasiswa harus menguasai kemampuan berbahasa Perancis ragamtulis yang baik dan benar sesuai dengan kaidah yang berlaku. Penelitian ini bertujuanuntuk menjelaskan kesalahan sintaksis yang dilakukan oleh mahasiswa Program StudiSastra Perancis Universitas Padjadjaran. Data yang digunakan dalam penelitian ini diambildari sepuluh karangan mahasiwa yang duduk di semester lima dan tujuh dan dianalisisdengan menggunakan metode simak dan metode analisis padan referensial translasionaldengan pendekatan kualitatif. Hasil dari penelitian ini menunjukkan bahwa kesalahantertinggi yang dilakukan mahasiswa adalah kesalahan dalam penggunaan passé composédan imparfait sebesar 50%, kemudian diikuti oleh kesalahan dalam penggunaan pronomrelatif simple et composé ‘pronomina penghubung bebas dan turunan’ sebesar 30%, danterakhir kesalahan dalam penggunaan pronom COD et COI ‘pronomina COD dan COI’ sebesar20%. Faktor yang menyebabkan terjadinya kesalahan tersebut adalah faktor linguistikyang disebabkan oleh perbedaan kaidah tatabahasa bahasa Indonesia dan Perancis.Selain itu terdapat pula faktor sosiolinguistik yang disebabkan oleh jarangnya mahasiswamembiasakan diri mereka untuk mengaplikasikan bahasa Perancis di luar kelas.Kata kunci: kesalahan, sintaksis, bahasa Perancis, tulis, pengajaranABST RACTTo produce a French-language essay that can be understood by the general public,the students should master the ability of French language in good and true writing skill inaccordance with the applicable rules. The aim of this research is to explain the syntactic errorsmade by students of French literature’s program at University Padjadjaran. The sources ofdata are taken from the ten student essays who sit in the fifth and seventh semesters, and themethods used in this research are Observation Method ‘Metode Simak’ and the analyticalreference translational analysis method with a qualitative approach. The results of thisresearch indicate that the highest errors that students make are the use of passé composéand imparfait as much as fifty percent, followed by errors in the use of pronom relatifsimple et composé ‘the free and derivative pronouns’ thirty percent, and the last error is inthe use of pronom CO D et CO I ‘‘The COD and COI pronouns’ twenty percent. The factors thatcause those errors are a linguistic caused by the difference rules of Indonesian and Frenchgrammar. In addition there are also sociolinguistic factors caused by the rarity of studentsfamiliarize themselves to apply the French language outside the classroom.Keywords: Error, syntax, French language, writing, teaching

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

scholarly journals Identification and Quantification of Coumarins by UHPLC-MS in Arabidopsis Thaliana Natural Populations

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1804
Author(s):  
Izabela Perkowska ◽  
Joanna Siwinska ◽  
Alexandre Olry ◽  
Jérémy Grosjean ◽  
Alain Hehn ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
High Performance ◽  
Biological Activities ◽  
Natural Populations ◽  
Biologically Active ◽  
Stress Factors ◽  
Mass Spectrometry Analysis ◽  
Engineering Research ◽  
Spectrometry Analysis ◽  
Model Plant Arabidopsis Thaliana

Coumarins are phytochemicals occurring in the plant kingdom, which biosynthesis is induced under various stress factors. They belong to the wide class of specialized metabolites well known for their beneficial properties. Due to their high and wide biological activities, coumarins are important not only for the survival of plants in changing environmental conditions, but are of great importance in the pharmaceutical industry and are an active source for drug development. The identification of coumarins from natural sources has been reported for different plant species including a model plant Arabidopsis thaliana. In our previous work, we demonstrated a presence of naturally occurring intraspecies variation in the concentrations of scopoletin and its glycoside, scopolin, the major coumarins accumulating in Arabidopsis roots. Here, we expanded this work by examining a larger group of 28 Arabidopsis natural populations (called accessions) and by extracting and analysing coumarins from two different types of tissues–roots and leaves. In the current work, by quantifying the coumarin content in plant extracts with ultra-high-performance liquid chromatography coupled with a mass spectrometry analysis (UHPLC-MS), we detected a significant natural variation in the content of simple coumarins like scopoletin, umbelliferone and esculetin together with their glycosides: scopolin, skimmin and esculin, respectively. Increasing our knowledge of coumarin accumulation in Arabidopsis natural populations, might be beneficial for the future discovery of physiological mechanisms of action of various alleles involved in their biosynthesis. A better understanding of biosynthetic pathways of biologically active compounds is the prerequisite step in undertaking a metabolic engineering research.

scholarly journals The Mechanical Properties and Damage Evolution of UHPC Reinforced with Glass Fibers and High-Performance Polypropylene Fibers

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2455
Author(s):  
Jiayuan He ◽  
Weizhen Chen ◽  
Boshan Zhang ◽  
Jiangjiang Yu ◽  
Hang Liu
Keyword(s):  
Mechanical Properties ◽  
Glass Fiber ◽  
Damage Evolution ◽  
High Performance ◽  
High Performance Concrete ◽  
Glass Fibers ◽  
Ultra High Performance Concrete ◽  
Concrete Damaged Plasticity ◽  
The Difference ◽  
Experimental Values

Due to the sharp and corrosion-prone features of steel fibers, there is a demand for ultra-high-performance concrete (UHPC) reinforced with nonmetallic fibers. In this paper, glass fiber (GF) and the high-performance polypropylene (HPP) fiber were selected to prepare UHPC, and the effects of different fibers on the compressive, tensile and bending properties of UHPC were investigated, experimentally and numerically. Then, the damage evolution of UHPC was further studied numerically, adopting the concrete damaged plasticity (CDP) model. The difference between the simulation values and experimental values was within 5.0%, verifying the reliability of the numerical model. The results indicate that 2.0% fiber content in UHPC provides better mechanical properties. In addition, the glass fiber was more significant in strengthening the effect. Compared with HPP-UHPC, the compressive, tensile and flexural strength of GF-UHPC increased by about 20%, 30% and 40%, respectively. However, the flexural toughness indexes I5, I10 and I20 of HPP-UHPC were about 1.2, 2.0 and 3.8 times those of GF-UHPC, respectively, showing that the toughening effect of the HPP fiber is better.

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