scholarly journals Effects of stress during commercial hatching on growth, egg production and feather pecking in laying hens

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262307
Author(s):  
Louise Hedlund ◽  
Per Jensen

Every year, billions of egg layer chicks around the world are hatched under highly stressful, industrial circumstances. Here, it is investigated how the stressful procedure in the commercial hatchery, including incubation, hatching, processing, and transport affects the chicks with regards to traits relevant for the egg production industry. These traits were compared to those of a control group hatched in a small incubator and handled gently och quietly in a quiet room without any processing and transport. The chicks were weighed at hatch and at eight additional time points: 4 days, 1 week (w), 2 w, 3 w, 5 w, 8 w, 20 w and 25 w of age. Feather pecking was studied at 15 w of age and damages to the feathers and injuries on the comb and wattle were assessed at 25 w of age. From 19 w of age, eggs were collected on three days per week, counted and weighed. Chicks from a commercial hatchery had a lower hatch weight than control chicks (p<0.001). At 20 w of age, the weight of the commercial hatched chicks was still numerically lower, although this did not reach statistical significance. Commercially hatched chicks tended to show more feather pecking behaviour at 15 w of age compared to control chicks (p<0.1), although feather condition at 25 w of age showed the opposite pattern. Regarding production, commercially hatched chickens laid fewer (p<0.05) and smaller (p<0.05) eggs than chicks hatched and handled under calm circumstances. From this experiment, it is concluded that the stressful experience in the commercial hatchery has an overall negative effect on traits relevant for the industry.

2021 ◽  
Vol 10 (36) ◽  
pp. 115-118
Author(s):  
Érika Cristina Ferreira ◽  
Paula Fernanda Massini ◽  
Caroline Felicio Braga ◽  
Ricardo Nascimento Drozino ◽  
Neide Martins Moreira ◽  
...  

Introduction: Toxoplasmosis is a zoonosis that represents a serious public health problem, caused by Toxoplasma gondii, which affects 20-90% of the world human population [1,2]. It is a serious problem especially when considering the congenital transmission due to congenital sequels. Treatment with highly diluted substances is one of the alternative/complementary medicines most employed in the world [3,4]. The current ethical rules regarding the number of animals used in animal experimental protocols with the use of more conservative statistical methods [5] can not enhance the biological effects of highly diluted substances observed by the experience of the researcher. Aim: To evaluate the minimum number of animals per group to achieve a significant difference among the groups of animals treated with biotherapic T. gondii and infected with the protozoan regarding the number of cysts observed in the brain. Material and methods: A blind randomized controlled trial was performed using eleven Swiss male mice, aged 57 days, divided into two groups: BIOT-200DH - treated with biotherapic (n=6) and CONTROL - treated with hydroalcoholic solution 7% (n=7).The animals of the group BIOT-200DH were treated for 3 consecutive days in a single dose 0.1ml/dose/day. The animals of BIOT – 200DH group were orally infected with 20 cysts of ME49-T. gondii. The animals of the control group were treated with cereal alcohol 7% (n=7) for 3 consecutive days and then were infected with 20 cysts of ME49 -T. gondii orally. The biotherapic 200DH T. gondii was prepared with homogenized mouse brain, with 20 cysts of T. gondii / 100μL according to the Brazilian Homeopathic Pharmacopoeia [6] in laminar flow. After 60 days post-infection the animals were killed in a chamber saturated with halothane, the brains were homogenized and resuspended in 1 ml of saline solution. Cysts were counted in 25 ml of this suspension, covered with a 24x24 mm coverglass, examined in its full length. This study was approved by the Ethics Committee for animal experimentation of the UEM - Protocol 036/2009. The data were compared using the tests Mann Whitney and Bootstrap [7] with the statistical software BioStat 5.0. Results and discussion: There was no significant difference when analyzed with the Mann-Whitney, even multiplying the "n" ten times (p=0.0618). The number of cysts observed in BIOT 200DH group was 4.5 ± 3.3 and 12.8 ± 9.7 in the CONTROL group. Table 1 shows the results obtained using the bootstrap analysis for each data changed from 2n until 2n+5, and their respective p-values. With the inclusion of more elements in the different groups, tested one by one, randomly, increasing gradually the samples, we observed the sample size needed to statistically confirm the results seen experimentally. Using 17 mice in group BIOT 200DH and 19 in the CONTROL group we have already observed statistical significance. This result suggests that experiments involving highly diluted substances and infection of mice with T. gondii should work with experimental groups with 17 animals at least. Despite the current and relevant ethical discussions about the number of animals used for experimental procedures the number of animals involved in each experiment must meet the characteristics of each item to be studied. In the case of experiments involving highly diluted substances, experimental animal models are still rudimentary and the biological effects observed appear to be also individualized, as described in literature for homeopathy [8]. The fact that the statistical significance was achieved by increasing the sample observed in this trial, tell us about a rare event, with a strong individual behavior, difficult to demonstrate in a result set, treated simply with a comparison of means or medians. Conclusion: Bootstrap seems to be an interesting methodology for the analysis of data obtained from experiments with highly diluted substances. Experiments involving highly diluted substances and infection of mice with T. gondii should be better work with experimental groups using 17 animals at least.


2015 ◽  
Vol 58 (1) ◽  
pp. 85-91 ◽  
Author(s):  
M. Petek ◽  
E. Topal ◽  
E. Cavusoglu

Abstract. The aim of this study was to investigate the effects of age on pecking behaviour and plumage quality of free-range laying hens during first access to range. Six hundred 16-week-old layer pullets obtained from a commercial company were housed in a experimental free-range house. The experimental house consisted of a slat-and-litter floor with range area and was divided into three similar pens. The pullets were randomly assigned to a control and two experimental groups according to age at first access to range area. The birds were allowed into the range area for the first time at 18, 20 and 22 weeks of age in the groups of treatment I, treatment II and control, respectively. Pecking behaviour and plumage quality of the birds were measured for 8-week intervals from 24 to 48 weeks of age. Total feather damage significantly increased with age in all groups (P < 0.040, P < 0.006, P < 0.001). Compared to the control group, birds allowed into the range area earlier had less feather damage. The feather score of the tail body region in all groups was significantly greater (more damage) than in the others (P < 0.001). There were no significant differences for the total pecking behaviour of the birds in the groups at 24, 32, 40 and 48 weeks of age. Incidents of gentle feather pecking in every period were more frequent, while aggressive pecking was less frequent (P < 0.026, P < 0.007, P < 0.001). It can be said that access to range area as early as possible at the beginning of the laying period may be useful to reduce the negative effect of feather pecking in free-range layer chickens.


2021 ◽  
Vol 13 ◽  
pp. 35-43
Author(s):  
I.F. Gorlov ◽  
◽  
Z.B. Komarova ◽  
A.V. Rudkovskaya ◽  
D.N. Nozhnik ◽  
...  

Aim. Study of the effectiveness of the use of the feed additive "Mega HenOn" in the training of chickens of an industrial herd of the cross "Highsex brown" under heat stress. Materials and Methods. Evaluation of the efficiency was carried out for 32 weeks on industrial chickens in the amount of 70 heads. During the experiment, the egg production of chickens (daily), feed costs, and the category of eggs were taken into account. To determine the quality characteristics of edible eggs, GOST 31654-2012 “Edible hen eggs. Technical conditions". Results. The studied additive had a positive effect on the egg productivity of chickens, both during heat stress and during normalization of the temperature regime. At the age of 47-50 weeks (June-July, the ambient temperature during the daytime increased to 38-40°C), egg production dropped sharply, and in the control group it was 79.03%, and in the experimental group, despite the decrease, it exceeded this indicator by 3.63%. At the age of 51-58 weeks (July-August, the increase increased to 36°C), the decrease in egg production continued, but in comparison with the previous period, there was a certain trend towards recovery, which amounted to 85.51%, exceeding the control by 2.22%. The studied feed additive, to some extent, neutralized the negative effect of heat stress on the body of birds. In general, over the period of the experiment, the egg-laying intensity in the experimental group was 89.1%, and in the control group - 87.09%. The cost of producing 10 eggs in the experimental group is 1.29 kg, which is 0.07 kg less than in the control group. The yield of eggs of the category "superior" and "selected" in the experimental group exceeded the control by 0.53 and 16.89%. The yield of eggs of the II category turned out to be higher in the control group and amounted to 29.56%, which is higher than in the experimental group by 13.73%. Conclusion. The use of the innovative “Mega HenOn” feed additive, in the diets of chickens during the heat stress period, contributed to a more complete realization of the genetic potential of birds, due to the stabilization of metabolic processes in the body of birds, an increase in egg production, a decrease in the yield of culling eggs and, as a consequence, an increase in the level of profitability by 2.38%.


Author(s):  
Peter Haščík ◽  
Miroslava Kačániová ◽  
Marek Bobko ◽  
Jaroslav Pochop ◽  
Michal Mihok ◽  
...  

In the experiment were verified the application of probiotic preparation through a water supply for feeding of cock’s hybrids Ross 308, Hubbard JV and Cobb 500 in the chemical composition of the most valuable parts of the carcass. Probiotic was based on the strain Lactobacillus fermentum with containing of 1.109 cfu.g−1 and potentially components of maltodextrin and oligofructose in 1% concentration. Length of feeding period was 42 days. Cocks were fed an ad libitum with the same starter mixture HYD-01 to 21th day and from 22nd to 42nd day of feeding with mixture HYD-02 in powdery form. The average of protein content of breast muscle was highest in Hubbard JV hybrid (23.93 g.100 g−1), lower in Cobb 500 hybrid (23.90 g.100 g−1) and lowest in Ross 308 hybrid (23.73 g.100 g−1), without significant differences (P ≥ 0.05) between hybrids and hybrids groups. Effect of probiotics had increased the protein content (P ≥ 0.05) in breast muscle of Ross 308 and Cobb 500 cocks and at the Hubbard JV only lower doses application during the feeding. The average of fat content in 100 g of breast muscle was lowest in Cobb 500 hybrid (1.09 g), higher in Hubbard JV hybrid (1.28 g) and highest in Ross 308 hybrid (1.35 g). Effect of probiotic to reduce fat content in breast muscle of cocks was at Ross 308 hybrid (1.33 and 1.23 g.100 g−1), Cobb 500 hybrid (0.98 and 1.02 g.100 g−1) and in second experimental group at Hubbard JV hybrid (1.03 g.100 g−1) statistically significant (P ≥ 0.05) in compared with control group, but significantly (P ≤ 0.05) between hybrids Cobb 500 and Hubbard JV in the first test groups. The average of energy value in 100 g of breast muscle was highest in Hubbard JV hybrid (449.24 kJ), lower in Ross 308 hybrid (448.40 kJ) and lowest in Cobb 500 hybrid (441.45 kJ), without significant differences (P ≥ 0.05) between hybrids and hybrids groups. The average of protein content of the femur was highest in Ross 308 hybrid (18.56 g.100 g−1), lower in Cobb 500 hybrid (18.42 g.100 g−1) and lowest in Hubbard JV (17.54 g.100 g−1) without statistical significance (P ≥ 0.05). Significant differences (P ≤ 0.05) of the femur were found at lower dose of probiotics between cock’s hybrids Hubbard JV (16.95 g.100 g−1) and Ross 308 (18.48 g.100 g−1) in the protein content. The average of fat content in the femur was highest in Ross 308 hybrid (10.58 g.100 g−1), lower in Hubbard JV hybrid (10.51 g.100 g−1) and lowest in Cobb 500 hybrid (10.29 g.100 g−1) without significant differences (P ≥ 0.05) between hybrids and hybrids groups. The higher fat and protein content in 100 g of the femur in Ross 308 hybrid was ensured the highest energy value (710.88 kJ), lower in Cobb 500 hybrid (696.40 kJ) and lowest in Hubbard JV hybrid (689.77 kJ) without significant differences (P ≥ 0.05) between hybrids and hybrids groups. The verified probiotic preparation in the final analysis had not negative effect on the chemical composition of the most valuable parts of the carcase cock’s hybrids Ross 308, Hubbard JV and Cobb 500.


2020 ◽  
Vol 29 (2) ◽  
pp. 175-179
Author(s):  
Melania Macarie ◽  
Simona Bataga ◽  
Simona Mocan ◽  
Monica Pantea ◽  
Razvan Opaschi ◽  
...  

Background and Aims: The importance of sessile serrated lesions (SSLs) in the pathogenesis of colorectal carcinoma has been recently established. These are supposed to cause the so-called “interval cancer”, having a rapidly progressive growth and being difficult to detect and to obtain an endoscopic complete resection. We aimed to establish the most important metabolic risk factors for sessile serrated lesions. Methods: We performed a retrospective case-control study, on a series of 2918 consecutive patients who underwent colonoscopy in Gastroenterology and Endoscopy Unit, County Clinical Emergency Hospital, Târgu-Mureș, Romania between 1 st of January 2015-31 th of December 2017. In order to evaluate the metabolic risk factors for polyps’ development, enrolled participants were stratified in two groups, a study group, 33 patients with SSLs lesions, and a control group, 138 patients with adenomatous polyps, selected by systematic sampling for age and anatomical site. Independent variables investigated were: gender, smoking, alcohol consumption, obesity, arterial hypertension, diabetes, hypercholesterolemia, hypertriglyceridemia, hyperuricemia, nonalcoholic liver disease. Results: For SSLs the most common encountered localization was the right colon in 30.55% of cases. By comparative bivariate analysis between SSLs group and control group, it was observed that hypertension (p=0.03, OR 2.33, 95 %CI 1.03-5.24), obesity (p=0.03, OR 2.61, 95 %CI 1.08-6.30), hyperuricemia (p=0.04, OR 2.72, 95 %CI 1.28-7.55), high cholesterol (p=0.002, OR 3.42; 95 %CI 1.48-7.87), and high triglycerides level (p=0.0006, OR 5.75; 95 %CI 1.92-17.2) were statistically associated with SSLs development. By multivariate analysis hypertension and hypertriglyceridemia retained statistical significance. Conclusions: Our study showed that the highest prevalence of SSLs was in the right colon and hypertension and increased triglycerides levels were associated with the risk of SSLs development. These risk factors are easy to detect in clinical practice and may help identifying groups with high risk for colorectal cancer, where screening is recommended.


2018 ◽  
Vol 6 (1) ◽  
pp. 133-153
Author(s):  
Toufan Aldian Syah

Banking industry has a very important role in economic development in a country. Indonesia, which is the largest Muslim country in the world, certainly has the prospect of the development of Sharia Banking Industry is very good in the future. However, the development of Sharia Bank has been slowing down in recent years and the profitability of sharia comercial banking is still below the ideal value. This study aims to determine the internal factors and external factors that affect the profitability of Sharia Bank in the year of January 2012 until August 2017. The variables used in this study are ROA, Inflation, NPF, and BOPO. The data used is aggregate data of all Sharia Commercial Banks recorded at Bank Indonesia. Measurement of Statistic Description, F-Test, T-Test, Correlation Coefficient, Coefficient of Determination and Multiple Linear Regression using IBM SPSS 21 software. The results showed that significant negative effect of BI rate, NPF and BOPO was found, while Inflation variable showed negative but not significant. Overall, the above variables affect the ROA of 87.7%, while 12.3% is likely to be influenced by other factors.


Author(s):  
J.E. Azimova ◽  
E.A. Klimov ◽  
E.A. Naumova ◽  
Z.G. Kokaeva ◽  
A.I. Zaitseva ◽  
...  

Перспективным в изучении биомаркеров мигрени может быть многолокусный анализ, в частности, анализ частот сочетанных генотипов. Цель исследования - поиск составных генетических биомаркеров индивидуальной предрасположенности к мигрени, полученных на основе полиморфизмов генов, уже показавших статистическую значимость при однолокусном ассоциативном анализе. Методика. Обследовано 155 пациентов с мигренью (104 пациента с эпизодической мигренью, 51 - с хронической мигренью), наблюдавшихся в Университетской клинике головной боли (Москва). Все пациенты - представители белой расы, жители Московского региона. Возраст пациентов - 30-50 лет. Контроль составили 365 необследованных лиц (популяционный контроль). Выявление исследуемых 22 генов (всего 31 SNP) осуществляли методом ПЦР, ПЦР-ПДРФ, аллель-специфичной ПЦР и ПЦР в реальном времени. Выявление ассоциированных с мигренью сочетанных генотипов проводили с использованием программы анализа полигенных данных APSampler v3.6. Результаты. Выявлено 8 сочетанных генотипов с высокой статистически значимой ассоциацией с мигренью (ОШ>20,0). В состав сочетанных генотипов вошли гены: CCKAR, CCKBR, COMT, MTHFR, MTR, MTRR. Так же выявлено 4 защитных сочетанных генотипа (ОШ<0,02), основным в которых является ген MAOA. Заключение. Полученные данные об ассоциированных с мигренью сочетанных генотипах указывают на значимую роль в патогенезе заболевания 2 биохимических систем: 1) холецистокининергической системы, регулирующей выброс и обратный захват дофамина, и 2) фолатного цикла, в ходе работы которого гомоцистеин метаболизируется в метионин. Результаты, полученные в данном исследовании, позволяют говорить о защитной роли аллеля VNT:R4 гена MAOA.Multilocus analysis, specifically, analysis of combined genotype frequencies may be promising in studying migraine biomarkers. The aim of the study was to search for composite genetic biomarkers, which would predict individual predisposition to migraine, obtained on the basis of gene polymorphisms that have already shown a statistical significance in a single-locus associative analysis. Methods. 155 patients with migraine aging 41.7 ± 12.5 who had been followed up at the University Clinic of Headache, Moscow, were evaluated (104 patients with episodic migraine and 51 with chronic migraine). All patients were white and residents of the Moscow region. The control group included 365 unexamined individuals (population control). Identification of The 22 genes under study (total, 31 SNPs) were identified by PCR, PCR-RFLP, allele-specific PCR, and real-time PCR. Combined genotypes associated with migraine were identified using the APSampler v3.6 software for polygenic data analysis. Results. Eight combined genotypes were identified with a highly significant association with migraine (OR> 20.0). The combined genotypes included the CCKAR, CCKBR, COMT, MTHFR, MTR, and MTRR genes. Four protective combined genotypes were also identified (OS <0.02) with the MAOA gene as the major one. Conclusion. Our data on migraine-associated combined genotypes indicate a significant role in the migraine pathogenesis of two biochemical systems, i) the cholecystokininergic system that regulates the release and reuptake of dopamine, and ii) the folate cycle, where homocysteine is metabolized to methionine. The results obtained in this study suggest a protective role of the VNT: R4 allele of the MAOA gene.


Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).


Author(s):  
Ahsha Vaksalla

Pornography is a debatable subject, both in Malaysia and around the world. Some feel it is harmless while others feel it is damaging. Pornography can bring about its own impact to the viewers. In Malaysia, the trend of viewing pornography among youngsters’ has been increasing. Studies have also shown Pornography can lead to unhealthy behaviors as a result of too much viewing. This study was conducted to discover the addiction level and the consumption effects on students at a Malaysian University. The researchers used purposive sampling to distribute questionnaires. The Scale used was the Internet Pornography Addiction Test and the Pornography Consumption Effects Scale consist of Positive and Negative dimension used to conduct the research. The research population (N=120) was students’ from University Tunku Abdul Rahman of Malaysia. There were significant relationship between the addiction and consumption. There were significant differences between the Positive and Negative Dimension as well. The Negative Effect Dimension is higher than the Positive Effect Dimension. There were significant differences between male and female students towards pornography addiction. However, there were not any significant differences among the genders in internet consumption. Male scored higher than female in the Internet Pornography Addiction. There were no significant differences between gender in the Positive and Negative Dimension.


2020 ◽  
Vol 62 (1) ◽  
pp. 38-42
Author(s):  
Anna Kostiukow ◽  
Wojciech Strzelecki ◽  
Mateusz W. Romanowski ◽  
Marta Rosołek ◽  
Ewa Mojs ◽  
...  

Introduction: The study is aimed at drawing the attention of the medical environment to the mental health aspects of young patients as a factor that significantly influences the efficiency of their rheumatic disease treatment. Aim: This paper is to check the risk of depression among a group of adolescents and young adults with rheumatic diseases. Material and Methods: The study was conducted among a group of 68 late adolescents and young adults (18-22 years old) with rheumatic diseases. The control group consisted of 102 young people (18-22 years old) without a diagnosed chronic disease. Risk of depression was measured using a screening tool – the Kutcher Adolescent Depression Scale (KADS). Results: The analysis showed that the probability of depression in the study group was 35.3%. In the control group, this rate was 19.6%. The results were statistical significance (p=0.028). Conclusions: The results of this study prove that the risk of depression among adolescents and young adults with rheumatic diseases is significantly higher than in healthy young people. The highest risk of depression is related to feeling tired, fatigue, low energy levels and lack of motivation as well as feeling worried, nervous, panicky, tense, keyed-up and anxious.


Sign in / Sign up

Export Citation Format

Share Document