scholarly journals Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) Hiperglikemia Setelah Pemberian Ekstrak Etanol Daun Mimba (Azadirachta Indica A. Juss)

2021 ◽  
Vol 6 (2) ◽  
pp. 193-202
Author(s):  
Fani Fahriyansyah ◽  
Sri Isdadiyanto ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi
Keyword(s):  
Epithelial Cell ◽  
Azadirachta Indica ◽  
Kidney Tissue ◽  
Ethanol Extract ◽  
Tubular Epithelial Cell ◽  
Albino Rats ◽  
White Rats ◽  
Chronic Hyperglycemia ◽  
Neem Leaves ◽  
Cell Thickness

Diabetes melitus merupakan penyakit kelainan metabolic yang ditandai dengan hiperglikemia kronis yang disebabkan tidak cukupnya produksi insulin dan resistensi insulin. Diabetes telah diketahui dapat menyebabkan komplikasi berupa kerusakan pada jaringan ginjal. Mimba (Azadirachta indica A.Juss) memiliki kandungan antioksidan flavonoid yang mampu menangkal radikal bebas dan memperbaiki jaringan ginjal yang rusak akibat diabetes. Penelitian ini menggunakan 18 tikus putih yang menjadi 6 kelompok perlakuan. P0 (kontrol normal) merupakan tukus normal diberi aquades. P1(Kontrol negatif) merupakan tikus hiperglikemia diberi aquades. P2 (kontrol positif) merupakan tikus hiperglikemia diberi glibenklamid dosis 2,25 mg/ kg BB. P3, P4, dan P5 merupakan tikus hiperglikemia yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data penelitian kemudian dianalisis dengan uji Anova. Untuk data yang tidak terdistribusi normal diuji dengan uji Kruskal-Wallis. Hasil menunjukkan bahwa pemberian ekstrak etanol daun mimba tidak memberikan pengaruh nyata terhadap bobot ginjal, konsumsi air minum, diameter glomerulus, ruang kapsula bowman, tebal sel epitel tubulus proksimal, dan tebal sel epitel tubulus distal. Kesimpulan dari penelitian ini adalah ekstrak etanol daun mimba  mampu melindungi jaringan ginjal tikus putih dari paparan hiperglikemia Diabetes is  a metabolic disorder that treats chronic hyperglycemia caused by insufficient insulin production and insulin resistance. Diabetes has been  known to cause complications in the form of damage to kidney tissue. Neem (Azadirachta indica A.Juss) has flavonoid antioxidants that can ward off free radicals and repair kidney tissue damaged by diabetes. The purpose of the study was to examine the ethanol extract of neem leaves repairing kidney tissue. The study used 18 albino rats were divided into 6 treatment groups. P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract The research data were analyzed by ANOVA. The non-normally distributed data were analyzed by  Kruskal-Wallis. The results showed that the ethanol extract of neem leaves had no significant effect on kidney weight, water consumption, glomerular diameter, bowman's capsule space, proximal tubular epithelial cell thickness, and distal tubular epithelial cell thickness. The conclusion of this study was  the ethanol extract of neem leaves  was able to protect the kidney tissue of white rats from hyperglycemia.

scholarly journals Potensi Rumput Laut (Eucheuma cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus norvegicus)

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Joni Tandi ◽  
Niluh Puspita Dewi ◽  
Resky Chandra Wirawan ◽  
Megawati R. Surat
Keyword(s):  
Blood Glucose ◽  
Kidney Tissue ◽  
Ethanol Extract ◽  
Seaweed Extract ◽  
Average Value ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Eucheuma Cottonii

ABSTRACT   This study aims to determine the effect of a multilevel dose of ethanol extract of seaweed (Eucheuma cottonii J. Agardh) on the decreasing blood glucose levels and kidney tissue regeneration of hypercholesterolemia-diabetic male white rats. 24 rats were divided into 6 groups, namely groups 1-3 (normal control, negative control, and positive control/Glibenclamide, respectively) and groups 3-6 (seaweed extract doses of 100, 200, and 400 mg/kg BW per oral, respectively). Rats were induced by high fat diet and Streptozotocin. Histopathological picture of the kidney was observed by HE staining using 400 magnifications. Data scoring of the level of kidney damage were analyzed by One Way ANOVA at a 95% confidence level and a Least Significant Difference (LSD) test was conducted. Seaweed ethanol extract at a dose of 100 mg/kg BW was effective in reducing blood glucose levels with an average value of 122.25 mg/dL and effective in regenerating the kidney tissue of male white rats with an average value of damage of 1.9. Therefore, seaweed extract has potency to be developed and further investigated as an antidiabetic agent.

scholarly journals Immunomodulator of Ethanol Extracts of The Leaves Azadirachta indica Against Macrophage Peritoneal Cell in Mice Induced The Vaccine BCG

2018 ◽  
Vol 7 (1) ◽  
pp. 8
Author(s):  
Yogi Khoirul Abror ◽  
Evy Diah Woelansari ◽  
Suhariyadi Suhariyadi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Azadirachta Indica ◽  
Clinical Manifestations ◽  
Ethanol Extract ◽  
Cell Number ◽  
Bcg Vaccine ◽  
Control Group ◽  
Control Group Design ◽  
Neem Leaves

This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.

scholarly journals Neem Leaf (Azadirachta indica A. Juss) Ethanolic Extract on the Liver and Kidney Function of Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Irma Seriana ◽  
Muslim Akmal ◽  
Darusman Darusman ◽  
Sri Wahyuni ◽  
Khairan Khairan ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Herbal Medicines ◽  
Safety Evaluation ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Male Rats ◽  
Control Group ◽  
Tropical Plants ◽  
Neem Leaves ◽  
Liver And Kidney

Neem (Azadirachta indica A. Juss) is one of the tropical plants found in Indonesia that has been used to prevent and treat various diseases. This study aimed to investigate the effect of the ethanol extract of neem leaves on the concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine in male rats. Twenty-four male Wistar rats were randomly divided into four groups (T0, T1, T2, and T3) with 6 rats in each group. T0 is the control group, and T1, T2, and T3 are the treatment groups that were administered 100, 200, and 300 mg/kg body weight of neem leaf ethanolic extracts for 48 days, respectively. On day 49, blood samples were collected to measure the concentration of AST, ALT, creatinine, and urea followed by an evaluation of liver and kidney histology. The results showed that the ethanolic extract of neem leaves did not affect the concentration of AST, ALT, and creatinine, The ethanol leaves reduced extract on the urea concentration, no abnormal changes were observed in the liver and kidney organs. In the future, it is required to carry out a comprehensive safety evaluation of the neem leaf ethanol extract for herbal medicines.

scholarly journals Bactericidal effect of Neem (Azadirachta indica) leaf extract on Helicobacter pylori

2021 ◽  
Vol 42 (6) ◽  
pp. 1591-1597
Author(s):  
A. Saxena ◽  
◽  
P. Arivaradarajan ◽  
A.K. Mukhopadhyay ◽  
S.P. Nandi ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Neem Leaves ◽  
Gastric Pathogen ◽  
H Pylori

Aim: The aim of the present study was to investigate the antibacterial effect of ethanolic extract of neem (Azadirachta indica) leaf against Gram-negative, gastric pathogen, Helicobacter pylori (H. pylori). Methodology: Extracts of neem leaf were prepared in different solventslike hexane, dichloromethane, chloroform, ethyl acetate, acetone and ethanol. Antibacterial activity was estimated in terms of zone of inhibition by performing Agar cup diffusion assay. Depending on the diameter of zone of inhibition, ethyl acetate, acetone and ethanol extract of neem leaves were selected for Thin Layer Chromatography. The presence of photochemicals were detected using iodine fumigation. Elution Assay was done to detect the bioactive components of the ethanol extract. Results: Out of sixsolvents used, ethanol extract of neem leavesshowed the maximum zone of inhibition against H. pylori. TLC separation of ethyl acetate, acetone and ethanol extract of plant products showed dark brown bands of phytochemicals on silica-gel G 60 plates. The contact bioautography assay showed a zone of 15 mm. Elution assay and agar cup bioassay was performed against H. pylori and the loading spot showed a zone of 11 mm. Interpretation: The findings of the present study revealed the anti-bacterial potency of ethanolic extracts of neem (Azadirachta indica) leaf against Gram-negative gastric pathogen H. pylori. The ethanolic extract of neem leaf can be used as an effective natural remedy in combating H. pylori infection.

scholarly journals HEPATOSOMATIK INDEKS DAN DIAMETER HEPATOSIT MENCIT (Mus musculus L.) BETINA SETELAH PAPARAN EKSTRAK ETANOL DAUN MIMBA (Azadirachta indica Juss.)

Pro-Life ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Anisa Nurul Hasana ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Ethanol Extract ◽  
Liver Weight ◽  
Hepatosomatic Index ◽  
Neem Tree ◽  
Randomized Design ◽  
Neem Leaves ◽  
Hepatocyte Damage ◽  
Paraffin Method

Neem leaves is one part of the neem tree which can be used as a traditional medicinal herb. The purpose of this study was to examine the effect of neem leaf ethanol extract on hepatosomatic index (HSI) and hepatocyte diameter in female mice. The study used a Complete Randomized Design consist of 4 treatments and 5 replications including K (aquadest), P1, P2 and P3 (neem leaf ethanol extract with dose of 8.4,11.2 and 14 mg/ kg BW/day. The treatment was administered orally with a volume of 0.2 mL for 21 days. Body weight were measured every 7 days. The liver was isolated, weighed and histologically prepared with paraffin method, HE staining and 5 μm incision thickness. HSI value determined from body weight and liver weight. Hepatocyte diameter were measured on 7 hepatocytes per lobule. The data was analyzed using ANOVA at 95% confidence level. The results showed that neem leaf ethanol extract had no significant effect (p> 0,05) on  body weight, liver weight, HSI value and hepatocyte diameter, but the liver microanatomy showed hepatocyte damage. It can be concluded that exposure of neem leaf ethanol extract for 21 days still safe to be used for reference of traditional medicine. Keywords: Neem leaf, HSI, hepatocyte diameter

scholarly journals HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS

2020 ◽  
Vol 8 (2) ◽  
pp. 92
Author(s):  
Irene Teh Kai Xin ◽  
Hani Plumeriastuti ◽  
Chairul Anwar ◽  
Kadek Rachmawati ◽  
Suzanita Utama ◽  
...  
Keyword(s):  
Heat Stress ◽  
Epithelial Cell ◽  
Broiler Chicken ◽  
Kidney Tissue ◽  
Tubular Epithelial Cell ◽  
Control Group ◽  
Adaptation Period ◽  
Histopathological Changes ◽  
Cell Necrosis ◽  
Scoring Method

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 

scholarly journals PENGARUH PEMBERIAN SALEP EKSTRAK ETANOL DAUN PILADANG(Solenostemonscutellarioides(L). Codd) SELAMA 15 HARI SECARA TOPIKAL TERHADAP AKTIVITAS PENYEMBUHAN LUKA EKSISI PADA TIKUS PUTIH JANTAN

2020 ◽  
Vol 9 (2) ◽  
pp. 192
Author(s):  
BA Martinus
Keyword(s):  
Wound Healing ◽  
Data Analysis ◽  
Epithelial Cell ◽  
Test Preparation ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Group Iii ◽  
White Rats ◽  
Solenostemon Scutellarioides ◽  
Group I

ABSTRACTA study has been conducted on the effect of ethanolic extract of piladang leaf (Solenostemon scutellarioides (L.) Codd) topically for 15 days on excision wound healing in male white rats. This study consisted of 5 groups of each group consisting of 3 rats, ie group I as control (Vaselin Flavum), Group II as comparator (Betadine® ointment), group III, IV, and V were the group given test preparation topical with an ointment concentration of 2%, 4% and 8% extract. The experimental animal was injured, then the initial wound was measured after it was applied to the back of the mouse 2 times a day given at 8 am and 4 pm for 15 days. On the 15th day, parameters such as percentage of wound healing and hydroxyproline measurement were performed, while epithelial time was performed every day until epithelial cell was formed. The results of data analysis using one way varian (ANOVA) showed that ethanol extract of pyuridic leaf can heal wound continued duncan test (SPSS 16.0) showed that there were significant differences between groups on wound healing parameters, wound healing percentage and hydroxyproline determination (p <0, 05), so it can be concluded that the preparation of leaf pyramid extract with 8% concentration more effective to give effect in wound healing. 

scholarly journals Post-natal development of rats` offspring treated with the ethanol extract of Neem leaves (Azadirachta indica A. Juss) during pregnancy and lactation

2015 ◽  
Vol 37 (2) ◽  
pp. 219
Author(s):  
Vanessa Carla Lima da Silva ◽  
Francine Maria de França Silva ◽  
Isabelle Maria Jacqueline Meunier ◽  
Tânia Maria Sarmento da Silva ◽  
Valdemiro Amaro da Silva Júnior ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Ethanol Extract ◽  
Neem Leaves ◽  
Pregnancy And Lactation

Intranuclear Crystals in Regenerating Canine Kidneys

1973 ◽  
Vol 31 ◽  
pp. 412-413
Author(s):  
D.G. Osborne ◽  
L.J. McCormack ◽  
M.O. Magnusson ◽  
W.S. Kiser
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Tubular Epithelial Cell ◽  
Tubular Epithelial Cells ◽  
Cell Nuclei ◽  
Crystalline Structures ◽  
Small Crystals ◽  
Membrane Bound

During a project in which regenerative changes were studied in autotransplanted canine kidneys, intranuclear crystals were seen in a small number of tubular epithelial cells. These crystalline structures were seen in the control specimens and also in regenerating specimens; the main differences being in size and number of them. The control specimens showed a few tubular epithelial cell nuclei almost completely occupied by large crystals that were not membrane bound. Subsequent follow-up biopsies of the same kidneys contained similar intranuclear crystals but of a much smaller size. Some of these nuclei contained several small crystals. The small crystals occurred at one week following transplantation and were seen even four weeks following transplantation. As time passed, the small crystals appeared to fuse to form larger crystals.

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