scholarly journals Cold atmospheric plasma risk assessment: stem cells

2020 ◽  
Vol 7 (4) ◽  
pp. 93-96
Author(s):  
R. Jamshidi ◽  
K. Hajizadeh
Keyword(s):  
Stem Cells ◽  
Cold Plasma ◽  
Cell Damage ◽  
Atmospheric Pressure Plasma ◽  
Plasma Source ◽  
Health Condition ◽  
Modern Technology ◽  
Atmospheric Plasma ◽  
Cell Deformation ◽  
Jet Plasma

Regarding the fact that cell shape indicates cell health and is of particular importance in the evaluation of new therapies, in this study, stem cell deformation during Atmospheric Pressure Plasma (APP) treatment was investigated. Given that, cell deformation is a warning of cell damage, it is therefore expected that APP-based therapy, a new modern technology that is expanding worldwide, will not lead to the deformation of normal cells. Here, the stem cells exposed to Helium-fed jet plasma, with two di erent powers of 15 and 25W. Moreover, the duration of exposure was changed (30, 50, 70, and 90 seconds) to determine the most appropriate exposure time and voltage, which maintains stem cells’ health condition. First of all, it was found that cold plasma at low power does not change the shape and elongation of stem cells. Besides, it was found that if the power of a cold plasma source is 25W, it will raise cell growth rate. In this paper, the gas ow rate of the helium plasma jet was set to 3.9 liters per minute, and a plasma source frequency of 30kHz was selected.

Polyolefinic Surface Activation by Low and Atmospheric Pressure Plasma Treatments

2014 ◽  
Vol 805 ◽  
pp. 149-154
Author(s):  
M.A. Martinez ◽  
J. Abenojar ◽  
N. Encinas
Keyword(s):  
Surface Energy ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Atmospheric Pressure Plasma ◽  
High Strength ◽  
Atmospheric Plasma ◽  
Adhesive Bonds ◽  
Lightweight Structures ◽  
Low Surface Energy ◽  
Plasma Treatments

Polyolefins are increasingly used in the construction of lightweight structures. Due to their low surface energy, it is difficult to have a proper bond with adhesives and paints. By using cold plasma treatments, these surfaces can be activated through the formation of highly reactive functional groups that can promote high strength adhesive bonds. This paper compares the results of cold plasma treatments using two techniques (low pressure and atmospheric plasma torch) applied on polypropylene and high density polyethylene. The obtained data allow the demonstration of a higher effectiveness of atmospheric plasma, with a significant increase in surface energy in both materials.

Hetero-Structure Integration using an Atmospheric Plasma Treatment for Surface Preparation before its Interconnection

2011 ◽  
Vol 2011 (DPC) ◽  
pp. 000817-000835
Author(s):  
Barbara Charlet ◽  
B. Charlet ◽  
L. Di Cioccio ◽  
N. Rochat ◽  
O. Renault ◽  
...  
Keyword(s):  
High Performance ◽  
Water Drop ◽  
Atmospheric Pressure Plasma ◽  
Surface Preparation ◽  
Plasma Source ◽  
Atmospheric Plasma ◽  
Hetero Structure ◽  
Passive Circuits ◽  
Wet Process ◽  
Novel Approach

One of the open challenges, related to the heterogeneous devices stacking, is its surface preparation, in order to assure the optimal electrical connections, and finally achieve the high performance structures having the stacked chips and passive components. Several techniques, using dry or wet process, allow the surface activation and preparation before the successive vertical interconnection achievement. We report the implementation of an environment friendly method, developed for chips and passive circuits surface preparation before its interconnection. This method is based on the use of atmospheric-pressure plasma source generating the radicals flux in the nitrogen or noble gases, mixed with a small volume of reactive gases, in order to stimulate the surface reactions. The surface properties of various treated materials were characterised by water drop contact angle, FTIR and TXRF method. The results of these behaviours allow defining a dedicated flow chart, in order to apply this surface treatment for the self-positioning of small silicon chips on the connection pads of printed passive circuits. Optimised process was applied to the silicon ICs chips assembly on the flexible polymeric substrates with passive devices; furthermore the treatment potentiality was also proved for other type of substrates and materials used for hetero structure integration. The application example given in this study concerns a novel approach of FRID tag assembly.

scholarly journals Evaluation of cold atmospheric-pressure plasma against burn wound infections and gene silencing

2021 ◽  
Author(s):  
Ensieh Abbasi ◽  
Jalil Fallah Mehrabadi ◽  
Mohamadreza Nourani ◽  
Yasaman Nazar Namini ◽  
Siamak Mohammadi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Atmospheric Pressure Plasma ◽  
Virulence Gene ◽  
Atmospheric Plasma ◽  
Medical Community ◽  
Diffusion Method ◽  
Histopathological Study ◽  
Pressure Plasma

Background and Objectives: Non-thermal atmospheric-pressure plasma or cold plasma is defined as an ionized gas. This study aimed to investigate the effect of cold plasma on Pseudomonas aeruginosa strains. Also, the expression level of the alp virulence gene before and after treatment with cold plasma was compared with the Housekeeping gene gyrA. Materials and Methods: P. aeruginosa isolates recovered from hospitalized burn patients at Shahid Motahari Burns Hos- pital, Tehran, Iran. The Kirby Bauer disk diffusion method was used to determine the antimicrobial susceptibility test. Then, the antibacterial effect of atmospheric non-thermal plasma was evaluated on P. aeruginosa in as in vitro and in vivo studies at different times on Muller Hinton agar and in mouse model (treated by plasma every day/ 90 sec). The histopathological study was evaluated by Hematoxylin-Eosin staining. Data were analyzed using SPSS software by the Chi-square test and Pvalues less than 0.05 considered as statistically significant. Results: Results indicated that non-thermal atmospheric plasma inhibited the growth of P. aeruginosa. The non-thermal helium plasma accelerates wound healing for 6 days. Results showed that cold plasma decreased virulence gene expression alp after treatment. Therefore, cold plasma can be suggested as a complementary therapeutic protocol to reduce bacterial infection and accelerate wound healing and reduce the expression of virulence genes of pathogens. Conclusion: Cold plasma showed pathogen inhibitory properties of P. aeruginosa and virulence alkaline protease and wound healing properties in animal models, so this inexpensive and suitable method can be presented to the medical community to disinfect burn wounds and improve wound healing.

scholarly journals Effect of Atmospheric Cold Plasma Treatments on Reduction of Alternaria Toxins Content in Wheat Flour

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 704 ◽  
Author(s):  
Elizabet Janić Hajnal ◽  
Milan Vukić ◽  
Lato Pezo ◽  
Dejan Orčić ◽  
Nevena Puač ◽  
...  
Keyword(s):  
Wheat Flour ◽  
High Performance ◽  
Cold Plasma ◽  
Plasma Source ◽  
Second Order ◽  
Experimental Results ◽  
Atmospheric Plasma ◽  
Alternariol Monomethyl Ether ◽  
Plasma Treatments ◽  
Alternaria Toxins

Beside Fusarium toxins, Alternaria toxins are among the most commonly found mycotoxins in wheat and wheat products. Currently, investigations of possibilities of reduction of Alternaria toxins in the wheat-processing chain are limited. Therefore, the aim of this study was to explore the potency of cold atmospheric plasma treatments, as a new non-thermal approach, for reduction of alternariol (AOH), alternariol monomethyl ether (AME) and tentoxin (TEN) content in spiked white wheat flour samples. Samples were treated with plasma generated in the air during 30 s to 180 s, with an increment step of 30 s, and at four varying distances from the cold plasma source (6 mm, 21 mm, 36 mm and 51 mm). The reduction of the Alternaria toxins content in samples after treatment was monitored by high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The maximum reduction of the examined Alternaria toxins was obtained by treatment performed at 6 mm distance from the plasma source, lasting 180 s, resulting in reductions of 60.6%, 73.8% and 54.5% for AOH, AME and TEN, respectively. According to the obtained experimental results, five empirical models in the form of the second-order polynomials were developed for the prediction of AOH, AME and TEN reduction, as well as the temperature and the moisture content of the wheat flour, that gave a good fit to experimental data and were able to predict the response variables successfully. The developed second-order polynomial models showed high coefficients of determination for prediction of experimental results (between 0.918 and 0.961).

scholarly journals Cold Atmospheric Plasma-Activated Water Irrigation Induces Defense Hormone and Gene expression in Tomato seedlings

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Bhawana Adhikari ◽  
Manish Adhikari ◽  
Bhagirath Ghimire ◽  
Gyungsoon Park ◽  
Eun Ha Choi
Keyword(s):  
Environmental Stress ◽  
Cold Plasma ◽  
Atmospheric Plasma ◽  
Defense System ◽  
Tomato Seedlings ◽  
Air Jet ◽  
Pathogenesis Related ◽  
Feasible Option ◽  
Jet Plasma ◽  
The Impact

Abstract Plants are very vulnerable to pathogen attacks and environmental stress as they are exposed to harsh environments in natural conditions. However, they have evolved a self-defense system whereby reactive oxygen and nitrogen species (RONS) act as double-edged swords by imposing (at higher concentration) and mitigating (at lower concentration) environmental stress. Cold plasma is emerging as a feasible option to produce a variety of RONS in a controlled manner when amalgamate with water. Cold plasma activated/treated water (PAW) contains a variety of RONS at concentrations, which may help to activate the plant’s defense system components. In the present study, we examine the effect of cold atmospheric-air jet plasma exposure (15 min, 30 min, and 60 min) on the water’s RONS level, as well as the impact of PAW irrigation, (assigned as 15PAW, 30PAW, and 60PAW) on tomato seedlings growth and defense response. We found that PAW irrigation (priming) upregulate seedlings growth, endogenous RONS, defense hormone (salicylic acid and jasmonic acid), and expression of key pathogenesis related (PR) gene. 30 min PAW contains RONS at concentrations which can induce non-toxic signaling. The present study suggests that PAW irrigation can be beneficial for agriculture as it modulates plant growth as well as immune response components.

scholarly journals The Whitening Effect and Histological Safety of Nonthermal Atmospheric Plasma Inducing Tooth Bleaching

2021 ◽  
Vol 18 (9) ◽  
pp. 4714
Author(s):  
Seoul-Hee Nam ◽  
Byul Bo Ra Choi ◽  
Gyoo-Cheon Kim
Keyword(s):  
Alternative Energy ◽  
Soft Tissues ◽  
Inflammatory Responses ◽  
Color Change ◽  
Atmospheric Pressure Plasma ◽  
Atmospheric Plasma ◽  
Tooth Bleaching ◽  
Histological Evaluation ◽  
Light Sources ◽  
Tooth Color

Various light sources have been applied to enhance the bleaching effect. This study was to identify the histological evaluation in oral soft tissues, as well as tooth color change after tooth bleaching by nonthermal atmospheric pressure plasma (NAPP). Nine New Zealand adult female rabbits were randomly divided into three groups (n = 3): group 1 received no treatment; group 2 was treated with NAPP and 15% carbamide peroxide (CP), which contains 5.4% H2O2, and group 3 was treated with 15% CP without NAPP. Color change (ΔE) was measured using the Shade Eye NCC colorimeter. Animals were euthanized one day later to analyze the histological responses occurring in oral soft tissues, including pulp, gingiva, tongue, buccal mucosa, and hard and soft palates. Changes in all samples were analyzed by hematoxylin and eosin staining and Masson’s trichrome. Teeth treated with plasma showed higher ΔE than that obtained with bleaching agents alone. Overall, the histological characteristics observed no appreciable changes. The combinational treatment of plasma had not indicated inflammatory responses as well as thermal damages. NAPP did not cause histological damage in oral soft tissues during tooth bleaching. We suggest that NAPP could be a novel alternative energy source to conventional light sources for tooth bleaching.

scholarly journals Electrical Supply Circuit for a Cold Plasma Source at Atmospheric Pressure Based on a Voltage Multiplier

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2132
Author(s):  
Ovidiu S. Stoican
Keyword(s):  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Plasma Source ◽  
Electrical Circuit ◽  
Jet Formation ◽  
Voltage Multiplier ◽  
Voltage Output ◽  
Intermediate Output ◽  
Electrical Supply ◽  
Lower Voltage

A cold plasma source operating at atmospheric pressure powered by a voltage multiplier is reported. In addition to its usual high voltage output, there is an intermediate output of lower voltage and higher current capability. A discharge current is drawn from both outputs. The ratio of the current supplied by each output depends on the operating state, namely, before or after the plasma jet formation. The electrical circuit is equivalent to two dc sources connected in parallel, used to initiate and sustain the electrical discharge. The plasma source is aimed to study the effect of cold plasma on the surface of various liquid or solid materials, including polymers.

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