scholarly journals СКРИНИНГ СОДЕРЖАНИЯ И ДИНАМИКА НАКОПЛЕНИЯ ПОЛИФЕНОЛЬНЫХ ВЕЩЕСТВ НЕКОТОРЫХ ВИДОВ БАЗИДИОМИЦЕТОВ

2015 ◽  
Vol 5 (03) ◽  
pp. 42-54
Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov
Keyword(s):  
Pleurotus Ostreatus ◽  
Total Content ◽  
Culture Fluid ◽  
Biomass Accumulation ◽  
Polyphenolic Compounds ◽  
High Positive Correlation ◽  
Fomes Fomentarius ◽  
Agrocybe Cylindracea ◽  
Laetiporus Sulphureus ◽  
Dry Biomass

<p>The <strong><em>aim </em></strong>of the study was to investigate the total content of polyphenolic substances in Basidiomycetes carpophores from 50 species, of which 27 belong to the order <em>Polyporales</em> and 23 to the order <em>Agaricales</em>. Introduced 23 strains of 8 species of Basidiomycetes. <strong><em>Methods.</em></strong> Gathered wild carpophores dried and crushed to a particle size of 0,1 till 0,01 mm and searching strains were cultured in Erlenmeyyers flasks by surface method on standard glucose-peptone culture medium. Determination of total content of polyphenolic compounds was carried out in ethanol extracts of mycological material by a modified method of Folin-Chokalteu. Completely dry biomass of carpophores and mycelium was determined gravimetrically. <strong><em>Results.</em></strong> There was identified the species of polyporal fungi <em>Ganoderma applanatum</em>, <em>Ganoderma lucidum</em>, <em>Laetiporus sulphureus</em> and <em>Fomes fomentarius</em> and types of agarical mushrooms <em>Stropharia rugosoannulata</em>, <em>Agrocybe cylindracea</em>, <em>Tricholoma flavovirens</em>, <em>Flammulina velutipes</em>, <em>Pleurotus ostreatus</em> and <em>Fistulina hepatica</em> high in polyphenolic compounds. It was determined the content of polyphenols ranging from more than 60 mg / g completely dry biomass. For introduced strains established dynamics of growth and accumulation of polyphenolic compounds in the mycelium and culture filtrate during fermentation on glucose-peptone medium. All cultures reach a maximum accumulation of biomass on the 12th day of growth. <em>Shizophyllum commune</em> Sc-1101 and 10 and <em>F. velutipes </em>F-202 have been identified as the most productive strains. The lowest accumulation of absolutely dry biomass was recorded for strain <em>P. ostreatus</em> P-192 and strain <em>F. fomentarius</em> Ff-09. Cultures have investigated individual value growth such as biomass accumulation in the applied cultivation conditions, which probably reflects the suitability of the medium for their growth and genotypic characteristics. Strains are overwhelmingly able to accumulate polyphenolic compounds in both mycelium and culture fluid during the whole period of cultivation. Maximum content of polyphenols in the mycelium to 96%, and in the culture fluid - for 91% of strains coincided with the end of their term cultivation. Calculated correlation coefficient between the content of polyphenols in the mycelium and culture fluid showed that there is a very high positive correlation of 73.2%, a positive high at 17.4% and the average 4.5% of experiment data. <strong><em>Conclusion.</em></strong> The strains of species <em>Shizophyllum</em> <em>commune</em>, <em>Pleurotus ostreatus</em>, <em>Fistulina hepatica</em> and <em>Laetiporus sulphureus</em> were selected for further research in order to obtain polyphenols mycelial and extracellular origin.<em></em></p> <p><em>Key words:</em> <em>polyphenols, Basidiomycetes, carpophores, mycelium, cultural filtrate</em></p>

scholarly journals ВПЛИВ ПЕВНИХ ВІТАМІНІВ НА СИНТЕЗ ПОЛІФЕНОЛЬНИХ РЕЧОВИН ДЕЯКИМИ БАЗИДІОМІЦЕТАМИ

2013 ◽  
Vol 3 (03) ◽  
pp. 24
Author(s):  
А. К. Veligodska ◽  
О. V. Fedotov
Keyword(s):  
Culture Filtrate ◽  
Polyphenolic Compounds ◽  
Fomes Fomentarius ◽  
Laetiporus Sulphureus ◽  
Dry Biomass ◽  
Species Effect

<p>We studied the influence of certain vitamins on the intensity of the synthesis of polyphenolic compounds and carotenoids by some Basidiomycetes strains, such as <em>Laetiporus sulphureus</em> Ls-08, <em>Fomes fomentarius </em>Ff-1201 and <em>Fistulina hepatica</em> Fh-18. The registration of accumulation of dry biomass and content of polyphenols and carotenoids in the mycelia and culture filtrate of strains that were cultivated on glucose-peptone substrates (GPS) with vitamins was performed. The vitamins A, E, C, B<sub>1</sub>, B<sub>12</sub>, and PP at the concentration of 0.005, 0.01 and 0.05 g/l were applied as modification of GPS. We founded the species effect on the synthesis of vitamins, polyphenols, and carotenoids. We suggested separate application of vitamins A, E, B<sub>1</sub>, and B<sub>12</sub> at concentration of 0.01 g/ l to induce the synthesis of polyphenols and carotenoids. Results of the study will be used to develop a modification of GPS for the cultivation of strains of polyphenolic substances of basidiomycete origin.</p> <p><em>Keywords: Basidiomycetes, mycelium, culture filtrate, polyphenols, carotenoids, vitamins.</em></p>

scholarly journals Migration of Hop Polyphenols in Beer Technology: Model Solution for Various Hopping Methods

2021 ◽  
pp. 628-638
Author(s):  
Marina Gernet ◽  
Irina Gribkova ◽  
Olga Borisenko ◽  
Maxim Zakharov ◽  
Varvara Zakharova
Keyword(s):  
Formation Rate ◽  
Influence Factors ◽  
Total Content ◽  
Production Method ◽  
Polyphenolic Compounds ◽  
Point Of View ◽  
Yeast Cells ◽  
Colloidal System ◽  
Migration Routes ◽  
Brewing Yeast

Introduction. The research featured the effect of various hopping conditions on the content of polyphenolic compounds associated with the extraction and biotransformation of hop compounds. This mechanism is responsible for uncharacteristic beer flavor in the traditional production method. The research objective was to study the migration routes, influence factors, and changes in the content of hop polyphenols in model experiments in order to reduce various factors in the production process chain. The experiment was important from the point of view of identifying the polyphenols contribution to the beer colloidal system. Study objects and methods. The study involved granulated aromatic hop of Tetnanger variety harvested in 2019, aqueous and 4% aqueous-alcoholic solutions simulating the wort and young beer liquid phase, and brewing yeast Sacharomyces cerevisiae of races Rh (lager) and Nottingham (ale). The work used the generally accepted methods for assessing the content of polyphenolic compounds. Results and discussion. The research established various factors that affected the migration of hop polyphenolic groups. The acidity effect on the polyphenol was established as follows: pH 4.4 contributed to a 12% greater isoxanthohumol accumulation, while pH 5.2 promoted a six times greater accumulation of anthocyanogens than pH 4.4. The total content of polyphenols during boiling was constant and correlated with the phenolic compound in different groups. The conditions of “dry” hopping, simulating the wort clarification in Wirpool, increased the dissolution of anthocyanogens by six times in comparison with kettle hopping, which was associated with the turbulent flow. The isoxanthohumol sorption and formation rate during “dry” hopping was established when modeling the maturation conditions for different temperatures, oxygen levels, and yeast races. A lower temperature (5°C) had a negative effect on the isoxanthohumol sorption. The quercetin content was found to be in the range of 0.9–2.0 mg/dm³ at 5°C and 0.8–4.7 mg/dm³ at 20°C, which determined the temperature effect on extraction during “dry” hopping. The presence of yeast cells in the medium promoted the quercetin accumulation: the quercetin content doubled at 5°C and quadrupled at 20°C compared with the control. The rutin content in the control increased for two days, and minor fluctuations in the content of yeast cells were 5.0 ÷ 7.4 mg/dm3. A comparative analysis of the simple phenolic acids and aldehydes amounts under “dry” hopping conditions showed a greater decrease in their concentration because they were involved in the yeast consumption and biotransformation processes. Conclusion. The research made it possible to establish the phenolic compounds in various groups of migration routes under the conditions of classical (kettle) and “dry” methods of hopping, as well as their dependence on such factors as medium acidity, stirring intensity, temperature, oxygen content, and yeast race. The sorption rates of the polyphenolic compounds were established as follows: absorption of isoxanthohumol was at its highest during the first day of “dry” hopping, and that of rutin – within two days, while quercetin was not absorbed at all. Therefore, an additional fermentation stage can be considered as the most expedient method of “dry” hopping.

scholarly journals Development of a method for assessing the structural heterogeneity of a population of different strains of tick-borne encephalitis virus

2018 ◽  
pp. 108-111
Author(s):  
К.К. Тучинская ◽  
В.П. Волок ◽  
В.В. Илларионова ◽  
О.И. Ковалева
Keyword(s):  
Total Concentration ◽  
Surface Protein ◽  
Total Content ◽  
Culture Fluid ◽  
Structural Heterogeneity ◽  
Encephalitis Virus ◽  
Virus Particles ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Different Strains

Вирус клещевого энцефалита (ВКЭ) является возбудителем тяжелого неврологического заболевания человека и широко распространен на территории Евразии. При репродукции флавивирусов помимо инфекционных вирионов накапливается набор неинфекционных вирусных структур: незрелые формы вирионов, пустые формы (не содержащие геном вирусные частицы), а также агрегаты поверхностного белка Е, способные оказывать влияние на иммунный ответ, и патогенез. Штаммы ВКЭ могут различаться по соотношению этих форм в инфекционном материале, т.е. по характеру структурной гетерогенности. Цель: подобрать комплекс методов, способных выявить данные различия. Методы. Общую концентрацию белка Е определяли методом ИФА, число частиц, содержащих геном (ГСЧ) - ПЦР в реальном времени, а для выявления инфекционных вирусных частиц - титрование в культуре клеток. Результаты. Разработан метод оценки структурной гетерогенности популяции ВКЭ. Было показано, что повышенное содержание неинфекционных вирусных частиц, содержащих геном, и несвязанных с ними белка Е не зависит от подтипа вируса. Выводы. Штаммы ВКЭ отличились по соотношению общего числа ГСЧ к числу инфекционных вирионов и по содержанию белка Е, связанного и несвязанного с ГСЧ. Tick-borne encephalitis virus (TBEV) is widespread in Europe and Asia and causes severe neurological disease in humans. It has been established that the reproduction of flaviviruses leads to the accumulation of a whole set of non-infectious viral structures aside from infectious virions. These structures include immature virions, empty forms (containing no genome) and aggregates of the surface protein E. These structures, despite being non-infectious, are able to influence the immune response and, consequently, the pathogenesis of TBEV infection. The aim of this work was to select a set of methods which can be implemented to identify these differences. Methods. Virus samples were analised for protein E concentration, number of genome-containing particles and infectivity. The total concentration of protein E in samples was evaluated using ELISA. The number of genome-containing particles was determined by a real-time PCR, and to assess the number of infectious virus particles titration in PEK cell culture was used. Results. An assay for total concentration of protein E in culture fluid of cells infected with different strains of TBEV based on the commertially available ELISA kit was developed. TBEV strains used in the study varied by the ratio of genome-containing particles to infectious virions. The amount of protein E not associated with genome-containing virions was calculated as a difference between total content of protein E and the amount of protein E bound to genome-containing particles. This amount was also different for studied samples of TBEV strains. Conclusion. No correlation was observed between the increased content of non-infectious genome-containing particles or the amount of residual protein E and TBEV subtypes.

scholarly journals Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3648
Author(s):  
Lucia Godočiková ◽  
Eva Ivanišová ◽  
Grzegorz Zaguła ◽  
Luis Noguera-Artiaga ◽  
Ángel A. Carbonell-Barrachina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Content ◽  
Composition Analysis ◽  
High Positive Correlation ◽  
Antioxidant Power ◽  
Single Origin ◽  
Ferric Reducing Antioxidant Power

The biological activity of chocolates gains more and more attention of consumers. Its antioxidant properties depend, among other factors, mainly on the origin of cocoa and the characteristics that this origin gives to the final product. Therefore, the aim of the study was to measure and compare the total content of polyphenols, antioxidant activity, and key odorants of commercial chocolates made from blend cocoa with single-origin ones. The highest content of polyphenols was found in 90% blend cocoa chocolate and single-origin samples, while the lowest content was exhibited by 100% chocolate from blend cocoa mass. The highest antioxidant activity measured by 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays was observed in the sample of chocolate with 90% cocoa solids from blend mass, followed by single-origin chocolates. A high positive correlation between ABTS assay and the total polyphenol and phenolic acids’ content, as well as among the total content of polyphenols, flavonoids, and phenolic acids was found. Mineral composition analysis showed that dark chocolate is a valuable source of some elements, especially Mg, Fe, and Zn. Potentially toxic elements were not detected or below permitted limits. Moreover, it was noticed that the main volatile compound in all tested samples was acetic acid, but pyrazines were considered the most important group of chocolate odorants.

scholarly journals The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 178 ◽  
Author(s):  
Izabela Grzegorczyk-Karolak ◽  
Katarzyna Hnatuszko-Konka ◽  
Mariola Zarzycka ◽  
Łukasz Kuźma
Keyword(s):  
Therapeutic Potential ◽  
Shoot Culture ◽  
Total Content ◽  
Biomass Accumulation ◽  
Hormonal Control ◽  
Polyphenolic Compound ◽  
Physiological Processes ◽  
Best Response ◽  
Alternative Sources ◽  
Metabolite Content

The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

scholarly journals Direct and subsequent effect of compost and poultry manure on the bioavailability of cadmium and copper and their uptake by oat biomass

2008 ◽  
Vol 54 (No. 7) ◽  
pp. 271-278 ◽  
Author(s):  
A. Hanč ◽  
P. Tlustoš ◽  
J. Száková ◽  
J. Habart ◽  
K. Gondek
Keyword(s):  
Correlation Coefficient ◽  
Avena Sativa ◽  
Organic Materials ◽  
Poultry Manure ◽  
Total Content ◽  
Cd Uptake ◽  
Dry Biomass ◽  
Subsequent Effect ◽  
Oat Biomass ◽  
Cu Uptake

Direct and subsequent influence of added organic materials on changes of cadmium and copper bioavailability in soil and their accumulation in aboveground oat (<I>Avena sativa</I> L.) biomass was studied in a three-year experiment. Mineral NPK, poultry manure and two types of composts were used as fertilizers. The average portion of available cadmium and copper from their total content in soil in all treatments was 0.94% and 0.25%, respectively. After application of poultry manure the concentration of available Cd increased during experimental years, contrary to Cu. The Cd uptake increased proportionally with available Cd content in soil almost in all treatments during experimental years (correlation coefficient <I>R</I> = 0.54; <I>P</I> < 0.05). The Cu uptake was however different than Cd uptake, mainly in the second and third year. The lowest uptake of Cu was found in the first experimental year and was in close positive correlation with yields of dry biomass.

Pleurotus ostreatus requirements for P, K, Mg, and Mn in submerged culture

1988 ◽  
Vol 34 (5) ◽  
pp. 620-624 ◽  
Author(s):  
Wireko Manu-Tawiah ◽  
Antonio M. Martin
Keyword(s):  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Mycelial Growth ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Biomass Concentration ◽  
Linear Relationships ◽  
Dry Biomass ◽  
The Media ◽  
Extract Medium

The nutritional requirements of P, K, Mg, and Mn for the mycelial growth of the fungus Pleurotus ostreatus have been studied. Experiments were conducted in submerged culture using a synthetic medium and a peat-extract-based medium. Requirements for both additional phosphorus and additional potassium were detected, and supplementing the peat extract medium with potassium phosphate enhanced the mycelial growth. The addition of yeast extract and potassium phosphate, combined with the peat extract, produced the highest dry biomass concentration in this work, 6.8 g/L. Linear relationships were found between the dry biomass concentration of P. ostreatus and the concentration of KH2PO4 added to the media. No additional requirement for magnesium beyond that existing in the peat extract medium was detected. Supplementing this medium with manganese in the range of 0.1 to 0.15 g/L produced a slight increase in the growth.

scholarly journals Zirconia Nanoparticles Impact On Morphophysiological Data And Mineral Composition Of P. ostreatus

2015 ◽  
Vol 22 (2) ◽  
pp. 169-188
Author(s):  
Nelya V. Doroshkevich ◽  
Marina V. Frontasyeva ◽  
Viktor S. Doroshkevich ◽  
Olena S. Lygina ◽  
Artem V. Shylo ◽  
...  
Keyword(s):  
Physiological Activity ◽  
Culture Fluid ◽  
Biomass Accumulation ◽  
Weight Percent ◽  
Biological Tissues ◽  
Extracellular Proteins ◽  
Physiological Processes ◽  
Low Concentrations ◽  
Biomedical Technologies

Abstract Neutron activation analysis of the Pleurotus ostreatus showed that adding of solid solution of ZrO2-Y2O3 hydroxide and oxide (3 mol % Y2O3) nanoparticles of size 4 and 9 nm at a concentration of 0.2 weight percent in a nutrient medium (Czapek) alters the character of physiological processes in the biological tissues of the mushrooms. This is manifested in the form of a significant change in morphological and physiological characteristics of the mushrooms and the elemental composition of the dry biomass. In particular, it is shown that the intercalation of nanoparticles into the tissues of the mushrooms leads to an increase of 1.3-1.4 times (more than 2.6 g/dm3) of biomass accumulation (industrial strain HK 35) and decrease of 1.7-1.8 times (below 1.7-2.5 mg/mm3) of concentrations of extracellular proteins into the culture fluid at a substantially constant value of the acidity. It is shown that the addition of ZrO2+3 mol % Y2O3 nanoparticles of sizes 4 or 9 nm into tissue of mushroom at step of the mother mycelium in very small concentrations can alter effectively the chemical composition of the substances produced by the cells and consequently, its physiological activity. It is shown that the use of low concentrations of ZrO2 nanoparticles allow to increase the yield and resistance of crops to diseases up to 1.2-1.5 times, as well as in the long term can be used in biomedical technologies for the treatment of cancer diseases.

scholarly journals EFFECT OF NaCl ON HYEMATOCOCCUS PLUVIALIS PRODUCTIVITY AND CONTENT IN ITS CELLS OF PHOTOSYNTHETIC PIGMENTS, ACTIVE OXYGEN FORMS AND ASTAXANTINE

2018 ◽  
Vol 63 (3) ◽  
pp. 263-275 ◽  
Author(s):  
N. G. Averina ◽  
N. V. Kozel ◽  
R. A. Sherbakov ◽  
M. S. Radyuk ◽  
E. E. Manankina ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Haematococcus Pluvialis ◽  
Total Content ◽  
Dry Weight ◽  
Control Culture ◽  
Maximum Effect ◽  
Cell Diameter ◽  
Chl A ◽  
Dry Biomass ◽  
Β Carotene

The effect of NaCl (25, 50, 100, 200 and 300 mM) on the productivity of the Haematococcus pluvialis strain IBCE H-17 on such parameters as dry biomass, content of protein, photosynthetic pigments, as well as astaxanthin and reactive oxygen species (ROS) was studied. It was found that NaCl at low and medium concentrations (25, 50 and 100 mM) in the culture medium stimulated the accumulation of dry biomass during the first 7 days of cultivation on average 1,3 times as compared to the control (Rudik’s standard medium). After 12 days of incubation, stimulation averaged 33 % using 25 and 50 mM salt. The protein content on a dry weight basis fell, averaging 70 % of the control on the 7th day of incubation with 50–300 mM salt and 55 % on the 12th day for a salt concentration of 100–300 mM. When the algae was grown for 7 days on solutions containing NaCl, the total content of photosynthetic pigments – chlorophylls (Chl) a and b as well as the carotenoids neoxanthine, violaxanthin, lutein and β-carotene decreased. Chl b was more resistant to salinity than Chl a. Of all the pigments, NaCl exerts the greatest negative effect on β-carotene. Stress conditions created by NaCl led to the generation of ROS, in particular, after 7 days of cultivation the total ROS content in the “NaCl-100” variant was 1.7 times higher than that in the control culture and 3.0 times higher than the control in the 12-day culture. A significant positive effect of salinity on the content of astaxanthin was noted. The maximum effect was observed with 100 mM NaCl. After 7 days of incubation, the content of astaxanthin exceeded the control indices by 2.8 times, and after 12 days – by 20.5 times. The number of algae cells after 7 days of incubation in the “NaCl-100” variant decreased on average by 33 %, while the cell diameter increased by 29 %. 

scholarly journals EXOGENOUS PHYSIOLOGICALLY ACTIVE SUBSTANCES OF TRICHODERMA HARZIANUM 128 AND THEIR SYNTHESIS WHILE INTRODUCTION OF MICROMYCETES INTO COMPOSTED SUBSTRATE

2019 ◽  
Vol 29 ◽  
pp. 37-45
Author(s):  
S. М. Derkach ◽  
V. V. Volkohon ◽  
V. P. Horban
Keyword(s):  
Trichoderma Harzianum ◽  
High Performance ◽  
Culture Fluid ◽  
Culture Liquid ◽  
Cytokinin Activity ◽  
Active Growth ◽  
Dry Biomass ◽  
Physiologically Active Substances ◽  
Chicken Litter ◽  
Active Substances

Objective. To study the possibility of production of physiologically active substances by the as-sociation of micromycetes Trichoderma harzianum 128, which is used for enrichment composted substrates based on chicken litter. Methods. Microbiological, physiological, accumulative thin lay-er chromatography, high-performance liquid chromatography (HPLC / MS). Results. T. harzianum 128 produces a significant amount of physiologically active growth stimulanting substances. Soak-ing of corn seeds in the culture liquid of micromycetes association, diluted with water in 100– 10,000 times, provides a reliable growth stimulation of seedlings and indicates the absence of phy-totoxicity in microorganisms. The instrumental determination of the content of exogenous phyto-hormones in pre-purified and concentrated phytohormonal extracts shows a significant amount of auxins in a culture fluid — their total amount reaches 18.33 μg/g of dry biomass of the producer, and of cytokinins, in particular, isopentenylidenidine (5.6 μg/g of dry biomass) and zeatin (0.88 μg/g dry biomass). Association T. harzianum 128 in small quantities produces gibberellic acids — GK3 (0.34 μg/g dry biomass) and GK4 — 0.23 μg/g of dry biomass). Absorbent acid was also found in the culture fluid (5.3 μg/g dry biomass), but its amount is four times less than the cor-responding measures in the known strain T. viride F100001, which was used as a positive control in the studies. While the introduction of association T. harzianum 128 into the composted chicken litter substrate, the obtained compost shows high auxin and cytokinin activity. Conclusion. Phyto-hormones, which are produced by the micromycetes association of T. harzianum 128, can positively influence the growth and development of plants, play a protective role in adverse environmental conditions. After introduction of the investigated fungi association to a composted substrate on the basis of chicken litter it accumulates significant amounts of physiologically active substances of auxin and cytokinin action. Under these conditions compost acquires new qualitative features.

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