scholarly journals Мікробоносійство личинок Strongyloides westeri

2017 ◽  
Vol 19 (73) ◽  
pp. 3-6
Author(s):  
V.O. Yevstafieva ◽  
I.N. Shendryk ◽  
Yu.A. Gugosyan
Keyword(s):  
The Body ◽  
Genus Bacillus ◽  
Nutrient Media ◽  
Experimental Animals ◽  
Nematode Larvae ◽  
Cultural Properties ◽  
Strongyloides Westeri

The associations of parasites in animals are diverse both in taxonomic respect (helminthes, protozoa, viruses, bacteria) and due to pathology caused by them. Strongyloides are often associated with parasitocenosis of animals, forming close symbiotic links with pathogens of infections. Penetrating into the body percutaneous or alimentary, larvae of helminthes inoculate bacteria and viruses, thereby complicating the course of diseases. In order to study the probability of persistence of microorganisms in the body of Strongyloides westeri larvae, larva homogenate was sown to nutrient media. Isolated microorganisms differed in terms of appearance, pigmentation and cultural properties, forming on colonies of S- and R-forms on dense nutrient media. In the broth, turbidity was detected, the formation of a precipitate and a film on its surface. Bacteriological studies of Strongyloides westeri homogenate larvae identified 7 cultures of microorganisms that were identified as representatives of the families: Enterobacteriaceae (genera Enterobacter, Escherichia, Klebsiella, Proteus) Micrococcaeae (genus Streptococcus, Staphylococcus) Bacillaceae (genus Bacillus). In the homogenate samples of larvae of all experimental animals (100%), bacteria of genera were found: Enterobacter, Escherichia, Klebsiella, Bacillus, Staphylococcus, 60% identified bacteria of the genus Proteus, 20% of the genus Streptococcus. The conducted studies established the parasitocenotic connections of Stongyloides westeri larvae and the microorganisms of seven different genera belonging to three families. The results of the studies confirm the ability of nematode larvae of the species Strongyloides westeri to be bacterial reservoirs. Helminthes is one of the probable ways of getting microorganisms into the body of a receptive host.

Indicators of metabolism and biochemical status of blood in horses of Yakut breed in winter and spring seasons

2020 ◽  
pp. 52-65
Author(s):  
U. Khompodoeva ◽  
R. Ivanov
Keyword(s):  
Classical Method ◽  
Live Weight ◽  
Weather Conditions ◽  
The Body ◽  
Metabolic Energy ◽  
Temperature Changes ◽  
Experimental Animals ◽  
Spring Period ◽  
Biochemical Status ◽  
Adaptive Mechanisms

The results of researches on the peculiarities of metabolism in the body of horses of Yakut breed in the winter and spring periods under the environments of Central Yakutia have been presented in the article. Physiological experiments on the digestibility of the main nutrients of the feed have been carried out in the winter (February) and spring (April) periods under the environments of the physiological yard in the Yakutsk Scientific and Research Institute of Agriculture named after M. G. Safronov with using the classical method. Five mares and five geldings of Yakut breed have been selected for researches. The average live weight of mares was 378–410 kg, geldings 394–403 kg. The experimental animals had average fatness. It has been found a higher intake of energy and the main nutrients of hay as a mono feed in winter compared to the spring period. At the same time pregnant mares in winter have been consumed 103,8±3,12 MJ of metabolic energy per head/day, which was 27,3 % significantly higher than in the spring period – 75,4±2,18 MJ (P ≥ 0,999). Geldings have been consumed in the experiment 67,70±2,85 MJ, which was by 43,7 % higher than in the spring period – 38,05 ± 4,40 MJ of metabolic energy per head/day (P ≥ 0,999). Experimental animals have been housed in stalls; there was no energy expenditure for movement, so the amount of energy consumed in winter indicates the production of heat in the animal’s body. Pregnant mares per 100 kg of live weight have consumed 31,5±4,11 MJ of metabolic energy or 33,3 % more than in the spring period – 21,0±1,98 MJ; geldings have consumed 27,69±1,12, which was 41,78 % significantly higher than in the spring – 16,12 ± 0,75 MJ (P ≥ 0,999). The high intake of metabolic energy into the body of Yakut horses in winter is explained by the manifestation of adaptive mechanisms that affect the effective metabolism for survival, adapted to frequent changes in weather conditions and sharp temperature changes. The established differences in the biochemical parameters of blood serum of horses of Yakut breed are associated with changes in the consumption and digestibility of basic nutrients and energy, depending on the ambient temperature.

Studies on Tapeworm Physiology

1949 ◽  
Vol 26 (1) ◽  
pp. 1-15
Author(s):  
J. D. SMYTH
Keyword(s):  
Bacterial Infections ◽  
Detrimental Effect ◽  
Horse Serum ◽  
Body Cavity ◽  
The Body ◽  
Nutrient Media ◽  
Medium Strength ◽  
By Products ◽  
Histochemical Examination

1. Plerocercoid larvae of the pseudophyllidean cestode Ligula intestinalis from the body cavity of roach, were cultured in vitro at 40°C. in a variety of saline and nutrient media. About 65% of such cultures were aseptic. 2. During cultivation, larvae produced acid by-products (unidentified) and the pH fell rapidly. 3. The presence of these acid by-products slowed down development, or, if present in sufficient quantity, caused death. 4. In order to obtain development in nutrient media in a period (3 days) comparable to that required in a bird (the normal host) it was necessary to renew the medium 24-hourly. 5. 6% of the eggs produced from a worm cultured in horse serum were fertile. Fertile eggs were never obtained from larvae cultured in any other media. 6. Certain bacterial infections had no apparent detrimental effect on development, but others were toxic. 7. Some larvae underwent development in non-nutrient medium (¾ strength Locke's solution). The exact conditions under which this occurred was not determined. 8. Fragments (3 cm. long), of larvae or larvae with either scolex or posterior half removed, underwent development to the stage of oviposition in nutrient media. 9. Histochemical examination revealed that the plerocercoid larvae were almost fat-free. During cultivation, very large quantities of cytoplasmic fat were produced the quantity being proportional to the duration of cultivation. Fat was produced even under starvation conditions (i.e. during cultivation in saline) and can be considered a metabolic by-product. 10. The fresh plerocercoid contained great quantities of glycogen in the parenchyma and muscle regions. After cultivation in nutrient or saline media, considerable quantities were still present.

EVALUATION OF TOXIOLOGICAL INDICATORS OF MONTHMORRILLONITE CLAY FOR THE PURPOSE OF USING THEM AS ALUMOSILICATE SOBENT AGAINST THE NEGATIVE EFFECT OF MYCOTOXINS ON THE BODY OF ANIMALS AND BIRDS

2021 ◽  
Vol 1 (1) ◽  
pp. 72-78
Keyword(s):  
Experimental Studies ◽  
The Body ◽  
Montmorillonite Clay ◽  
Test Substance ◽  
Negative Effects ◽  
Experimental Animals ◽  
Eyelid Edema ◽  
White Rats ◽  
Mucous Membranes ◽  
Negative Effect

The article presents the results of laboratory studies to assess the toxicological parameters of montmorillonite clay from the Pogadaevskoye deposit in the West Kazakhstan region in order to use them as an aluminosilicate sorbent in the composition of feeds that reduce the negative effects of mycotoxins on the body of animals and birds. The relevance of research is associated with the cultivation of healthy and highly productive animals and poultry in order to ensure the food security of the Republic of Kazakhstan.The studies carried out to assess the toxicological parameters of montmorillonite clay in order to use them as an aluminosilicate sorbent in experimental animals (rabbits and white rats) allowed the following results to be obtained: Visual study of the intensity of erythema when exposed to the test substance on the skin of rabbits showed their absence (0 points). The study of the intensity of edema (an increase in the thickness of the skin clutch of rabbits) when exposed to the test substance on the skin of rabbits showed no reaction (0 points). Studies evaluating the irritating effect of the test substances on the mucous membranes of the eyes of rabbits by symptoms of damage showed the absence of hyperemia (0 points). Weak eyelid edema (1 point), the minimum amount of discharge in the corner of the eye (1 point). The results of studies on the classification assessment of the test substance for the severity of the irritant effect on the eyes of rabbits showed that the average total score of the severity of the irritative effect corresponds to 1 point. A comprehensive analysis of the results obtained on the basis of scientific and experimental studies to assess the toxicological indicators of montmorillonite clay from the Pogadaevskoye deposit in relation to irritating effects on the skin and mucous membranes of experimental animals (rabbits) showed their harmlessness.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals Удосконалення методів діагностики піометри у кішок за допомогою сонографії

2015 ◽  
pp. 182-185
Author(s):  
Д. О. Вусик
Keyword(s):  
The Body ◽  
Surrounding Tissue ◽  
Maximum Diameter ◽  
Experimental Animals ◽  
Healthy Female

Протягом 2008–2014 років в умовах ветеринарно-го центра «Хелс» м. Суми сонографічним методомбуло досліджено 221 кішку. Сонографічним мето-дом насамперед визначили ультразвукову харак-теристику морфологічного стану матки клінічноздорових кішок у період анеструсу. Ехограма здо-рової матки характеризується рівними та однорід-ними стінками, відсутністю візуалізації порожнини,середньої або незначно зниженою по відношенню дооточуючих тканин ехогенністю. Яєчники у здоровихкішок під час сонографічного дослідження не ві-зуалізувались. Ехограма матки в разі піометри ха-рактеризується розширенням рогів матки, потов-щенням або розширенням порожнини матки. Макси-мальний діаметр тіла та рогів матки становив від-повідно 2,4 см і 5,8 см. Розширення порожнини мат-ки відмічалося у всіх дослідних тварин у випадку піо-метри. Водночас у тварин ширина порожнини маткистановила 1,22 ± 0,35 см. Вмітиме порожнини маткимало гіпоехогенне або анехогенне відображення, вдеяких випадках відзначалася помірна гіпоехогенназернистість. During 2008–2014 years 221 cats were investigated with the help of sonographic method in Sumy veterinary center «Health». Primarily the ultrasound characteristic of morphological condition of the uterus of clinical healthy female of cats during anoestrus was determined by the sonographic method. Echogram of healthy uterus is characterized by equal and uniform walls, lack of visualization of cavities, medium or slightly reduced relative to the surrounding tissue echogenicity. The ovaries of healthy cats at sonographic research were not visualized. Sonogram of the uterus with pyometra is characterized by enlargement of uterine horns, thickening or enlargement of the uterus. The maximum diameter of the body and uterine horns made pursuant 2,4–5,8 cm. Expanding uterus was noted in all experimental animals with pyometra. This animal uterus width was 1,22±0,35 cm. Contents of uterus were hypoechoic or anechoic display, in some cases hypoechoic moderate graininess was marked.

scholarly journals Toxicity assessment of a technical product of the triazole class

2020 ◽  
Vol 99 (11) ◽  
pp. 1276-1279
Author(s):  
Valery N. Rakitskii ◽  
Tatiana M. Epishina ◽  
Elena G. Chkhvirkiya
Keyword(s):  
Body Weight ◽  
The Body ◽  
Male Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
High Biological Activity ◽  
Experimental Animals ◽  
Technical Product ◽  
White Rats ◽  
Toxicological Studies

Introduction. Historically, pesticides are evaluated more strictly from a medical point of view than other chemicals. Since their features, such as deliberate introduction into the environment, the possibility of contact with them by large masses of the population, and the high biological activity determine their potential danger to humans. Purpose of research - study of the biological effect of a technical product derived from triazoles when it is repeatedly ingested orally in mammals (rats), establishment of inactive and active doses, justification of the permissible daily dose (DSD) for humans. Material and methods. In acute experiments, white rats were used, including 6 animals in the group. Tested dose: 500-4000 mg/kg of body weight. A chronic (12 months) experiment was performed on 80 male rats with a bodyweight of 180-190 g at the beginning of the study. Tested doses: 5.0; 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental animals, 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water, and food consumption, recorded the timing of death, changes in body weight, physiological, biochemical, and hematological indices. Results. Indices of the acute oral toxicity on the studied product LD50 male rats were 2250 ± 483 mg/kg body weight. The dose of 5.0 mg / kg of body weight was not found to cause significant changes in all studied indices. The doses of 16.0 and 55.0 mg/kg of body weight had a polytropic effect on the body in experimental animals. Discussion. The studied product for the acute oral toxicity refers to low-hazard compounds, the doses of 16.0 and 55.0 mg/kg of body weight has a polytropic effect on the mammalian body, causing changes in carbohydrate, lipid, and lipoprotein metabolism in the body of rats - was accepted as acting. The dose of 5.0 mg / kg of body weight, when administered in rats, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. Based on the inactive dose-5.0 mg/kg of body weight and taking into account the reserve factor of 100, we have scientifically justified DSD for a person at the level of 0.05 mg/kg. Summary. The conducted sanitary and Toxicological studies indicate the need to assess the toxicity of new technical products to the mammalian body, to increase the reliability of the developed hygiene standards in environmental objects and food products.

scholarly journals Fruits of plants of Amelanchier genus (Amelanchier Medic) as source of biologically active substances and minerals

2018 ◽  
Vol 26 (2) ◽  
pp. 296-304
Author(s):  
Elena A. Laksaeva
Keyword(s):  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Erythrocyte Membranes ◽  
Human Organism ◽  
High Concentration ◽  
Experimental Animals ◽  
Polysaccharide Complex ◽  
Physiological Processes ◽  
Active Substances

An interest in plants of Amelanchier genus is stimulated by the necessity of search for plant sources rich in biologically active substances and possessing the ability to influence different physiological processes in a human organism. The article gives information about places of growth of different species of Amelanchier (Amelanchier Medic) and about the fact that its fruits contain high concentration of free sugars, relatively low level of organic acids, sufficient amounts of various vitamins and provitamins and a complex of mineral substances. Nutritive and biological value of Amelanchier fruits in complex may produce a beneficial effect on different biochemical and physiological processes of normal vital activity of a human organism. The data are given about a positive influence of enteral introduction of polysaccharides isolated from Amelanchier fruits, on different physiological processes that enhance adaptational reserves of an organism of experimental animals. In particular, it was shown that watersoluble polysaccharide complex (WSPC) of Amelanchier fruits activates erythropoiesis increasing the amount of erythrocytes and hemoglobin in blood of experimental animals and raises concentration of iron. WSPC improves physical working capacity and increases the body mass of experimental animals. Addition of watersoluble polysaccharide complex of Amelanchier fruits to blood of a healthy donor increases thermal, osmotic and peroxide resistance of erythrocyte membranes thus protecting cells against damage under action of adverse factors in the experiment. Fruits of plants of Amelanchier genus may be recommended to be used as a food additive or a product in dietary and prophylactic therapy.

To the doctrine of the intravital coloration of the reticuloendothelial system in experimental animals

1930 ◽  
Vol 26 (4) ◽  
pp. 438-438
Author(s):  
L. I. Vilensky
Keyword(s):  
Connective Tissue ◽  
Reticuloendothelial System ◽  
The Body ◽  
Experimental Animals ◽  
White Rats ◽  
Medical Societies ◽  
Physiological Properties ◽  
Reticulo Endothelial System

Meetings of medical societies. Society of Physicians at Kazan University. General Sessions Session 4 March 1930 L.I. Vilensky. Towards the doctrine of the intravital coloration of the reticuloendothelial system in experimental animals. On the basis of a study of 267 animals of various species (rabbits, rats, pigs, white mice, white rats and dogs) the vivo coloring of trypanblau, pyrrholblau, carmine and collargol, the author comes to the following conclusions: 1) All active cellular elements of the indifferent connective tissue that has retained the embryonic properties of the mesenchyme. 2) Teaching-Aschoff's, in the light of intravital coloration, should be expanded, as the teaching of an active mesenchyme, scattered throughout the body, but united by common physiological properties. 3) The process of gradual absorption of colloidal paint in individual elements of the reticulo-endothelial system has a certain pattern, which is exactly the same in different animals.

Effect of Bitters on the Body Weight, Lipid Profile, Catalase and Lipid Peroxidation in Experimental Animals

2012 ◽  
Vol 13 (1) ◽  
pp. 62-66 ◽  
Author(s):  
M.A. Alabi ◽  
R.M. Sunday ◽  
T. Olowokere ◽  
F.A. Kareem ◽  
F. Osanaiye
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Lipid Profile ◽  
The Body ◽  
Experimental Animals

On the larval migration of some parasitic nematodes in the body of the host and its biological significance

1929 ◽  
Vol 7 (1) ◽  
pp. 15-26 ◽  
Author(s):  
F. Fülleborn
Keyword(s):  
Pure Culture ◽  
Subcutaneous Tissue ◽  
Biological Significance ◽  
Culture Method ◽  
The Body ◽  
Parasitic Nematodes ◽  
Healthy Skin ◽  
Suitable Host ◽  
Nematode Larvae ◽  
Short Time

As Looss has shown, the larvæ of Ancylostoma can infect a suitable host by penetrating the healthy skin. If we place the infective material on the surface of the skin, after a short time we find the larvæ in the subcutaneous tissue. The same behaviour is shown by the filariform larvæ of Strongyloides siercoralis, which provide more convenient material for examination, because they can be collected very easily in pure culture and free from fluid by the special culture-method described by me (1924), the principle of which depends on the well-known fact that the filariform larvæ of Strongyloides and other nematode larvæ, accumulate in the form of white filaments of several millimetres in length (fig. 3), each consisting of thousands of individual larvæ, on little prominences in their vicinity, all being guided to similar places by the same sets of “tropisms” (Fülleborn, 1924).

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