scholarly journals Cell Viability of Candida albicans Against the Antifungal Activity of Thymol

2014 ◽  
Vol 25 (4) ◽  
pp. 277-281 ◽  
Author(s):  
Laís César de Vasconcelos ◽  
Fabio Correia Sampaio ◽  
Allan de Jesus dos Reis Albuquerque ◽  
Laurylene César de Souza Vasconcelos
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Cell Viability ◽  
Acrylic Resin ◽  
Mean Value ◽  
Denture Stomatitis ◽  
Minimum Fungicidal Concentration ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Syto 9

Candida albicans is a commensal fungus, but circumstantially it may cause superficial infections of the mucous membranes, such as denture stomatitis, when a biofilm is formed on the surface of dental prostheses. This study evaluated the cell viability of C. albicans biofilms against the antifungal activity of thymol when compared with miconazole, by the fluorescence imaging using SYTO 9 and propidium iodide dyes, and counting of colony forming units. C. albicans standard strains (ATCC 11006) were used. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of drugs were determined by broth microdilution tests and the inoculum was standardized to match 0.5 on the McFarland scale (106 cfu/mL). Biofilms were grown on the surface of acrylic resin disks in parallel flow chambers from Sabouraud broth supplemented with 10% dextrose. For counting of colony forming units, the fungal solution was sequentially diluted and plated in Sabouraud dextrose agar. Data were analyzed using two-way ANOVA and Tukey's test (a=5%). Biofilms treated with thymol and miconazole presented low numbers of viable cells at the evaluated exposure times. There was statistically significant difference (p<0.05) when compared with control, and the mean value of the exposure times between miconazole and thymol did not differ significantly (p>0.05). In conclusion, both drugs have similar efficiency as antifungal agents against biofilms of C. albicans formed on acrylic surfaces.

Pengaruh perendaman plat resin akrilik dalam ekstrak kayu manis (Cinnamomum burmanii) terhadap jumlah blastospora Candida Albicans

e-GIGI ◽  
2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Christian Dama
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
T Test ◽  
Control Group ◽  
Dilution Method ◽  
Denture Stomatitis ◽  
Cinnamon Extract ◽  
Acrylic Plate ◽  
Removable Denture ◽  
Significant Difference

Resin akrilik sering digunakan sebagai bahan dari gigi tiruan, khususnya basis gigi tiruan. Adanya rongga-rongga mikro pada akrilik menjadi tempat perlekatan sisa-sisa makanan yang dapat meningkatkan jumlah mikroorganisme dalam rongga mulut, salah satunya ialah jamur Candida albicans. Pertumbuhan yang pesat dari jamur Candida albicans menjadi penyebab utama infeksi pada mukosa rongga mulut pemakai gigi tiruan lepasan akrilik, disebut denture stomatitis. Ekstrak kayu manis yang mengandung minyak atsiri, sinamaldehid, eugenol dan juga senyawa seperti flavonoid, Saponin, serta tanin memiliki efek antifungi. Tujuan penelitian ini untuk mengetahui pengaruh dari ekstrak kayu manis dan konsentrasi ekstrak kayu manis terhadap jumlah blastospora Candida albicans pada plat akrilik sehingga mampu mencegah dan menanggulangi penyakit denture stomatitis. Plat akrilik yang telah terkontaminasi dengan jamur Candida albicans direndam dalam ekstrak kayu manis dengan konsentrasi 20%, 30%, 40%, 50%, dan aquades sebagai kontrol. selanjutnya, plat akrilik di getarkan untuk menjatuhkan Candida albicans dalam tabung reaksi dan dihitung jumlah blastosporanya dengan metode pengenceran menggunakan cairan NaCl dan metode hitung langsung pada mikroskop. Hasil perhitungan statistik dengan uji Independent t-test diketahui terdapat perbedaan yang bermakna antara kelompok ekstrak kayu manis dan kelompok kontrol (p ≤ 0,05). Dari hasil penelitian dapat disimpulkan bahwa ekstrak kayu manis dapat memengaruhi pertumbuhan jumlah blastospora Candida albicans pada plat resin akrilik dan meningkat seiring dengan meningkatnya konsentrasi ekstrak kayu manis (cinnamomum burmanii) yang digunakan.Kata kunci: Resin akrilik, Candida albicans, ekstrak kayu manis.ABSTRACTAcrylic resin is often used as an ingredient of artificial teeth, denture base in particular. The existence of micro cavities in acrylic attachment to place leftover food that can increase the number of microorganisms in the oral cavity, one of which is Candida albicans. The rapid growth of Candida albicans is a main cause of infection in the oral mucosa acrylic removable denture wearers, called denture stomatitis. Cinnamon extract that contains essential oils, sinamaldehid, eugenol and also compounds such as flavonoids, saponins, and tannins have antifungal effects. The purpose of this study to determine the effect of cinnamon extract and cinnamon extract concentrations against Candida albicans blastospora number on acrylic plate so as to prevent and control disease denture stomatitis. Acrylic plate that has been contaminated with Candida albicans soaked in cinnamon extract with a concentration of 20%, 30%, 40%, 50%, and sterile water as a control. furthermore, acrylic plate vibrate to drop Candida albicans in a test tube and counted the number of blastospora by using saline dilution method and direct count method on the microscope. The results of calculations with the statistical test known Independent t-test significant difference between cinnamon extract group and the control group (p ≤ 0,05). From the results of this study concluded that cinnamon extract may affect the growth of Candida albicans blastospora on acrylic resin plate and increases with increasing concentration of the extract of cinnamon (Cinnamomum burmanii) were used.Keywords : Acrylic resin, Candida albicans, cinnamon extract.

scholarly journals Titanium Dioxide Addition to Heat Polymerized Acrylic Resin Denture Base Effect on Staphylococcus aureus and Candida albicans

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Dwi Tjahyaning Putranti ◽  
Afrina Fadilla
Keyword(s):  
Staphylococcus Aureus ◽  
Titanium Dioxide ◽  
Candida Albicans ◽  
Tio2 Nanoparticles ◽  
Base Material ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Significant Difference ◽  
Almost All

  Introduction: Heat-polymerized acrylic resin is used to manufacture almost all denture bases. One of the disadvantages of acrylic resin is its porosity and surface roughness; food scraps attach easily. If the dentures are not cleaned, they become a place for microbial species development and cause denture stomatitis, with Staphylococcus aureus and Candida albicans as the major etiologic agents. Adding nanoparticles of titanium dioxide to heat-polymerized acrylic resin may provide antimicrobial activity to the acrylic resin base.   Objectives: This study aimed to determine whether adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base materials affected the amounts of Staphylococcus aureus and Candida albicans.   Methods: The samples in this study used heat-polymerized acrylic resin without the addition of TiO2 nanoparticles and with the addition of 2%, 3%, and 4% TiO2 nanoparticles on test bar sizes of 10 mm × 10 mm × 1 mm. There were 48 total test samples: 24 of Staphylococcus aureus and 24 of Candida albicans. The obtained data were analyzed by a one-way ANOVA test.   Results: The results showed that adding TiO2 nanoparticles influenced the amount of Staphylococcus aureus (p<0.05) and Candida albicans (p<0.05). A least significant difference test showed differences of effect in the amounts of Staphylococcus aureus when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base and there were differences in effect to amounts of Candida albicans when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base.   Conclusion: Adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base material affects the amounts of Staphylococcus aureus and Candida albicans.

DIFFERENCES IN THE NUMBER OF CANDIDA ALBICANS COLONIES ON ACRYLIC RESIN AND THERMOPLASTIC NYLON IN SOURSOP LEAF EXTRACT IMMERSION

2021 ◽  
Vol 17 (2) ◽  
pp. 123-131
Author(s):  
Ria Koesomawati ◽  
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Test Analysis ◽  
Denture Stomatitis ◽  
Treatment Groups ◽  
Significant Difference

Introduction: Denture stomatitis is a chronic inflammation caused by wearing dentures, mainly caused by Candida albicans. Heat-polymerized acrylic resins are often used as denture bases, but lack the ability to absorb liquids due to their porosity and surface roughness. The basis of the latest dentures is thermoplastic nylon because it is more aesthetically, hypoallergenic and more flexible. Soursop leaf extract contains alkaloids, tannins, flavonoids, and saponins as antifungal. The purpose of this study was to examinate whether there were differences in the number of Candida albicans colonies on heat-polymerized acrylic resin plates and thermoplastic nylon in soursop leaf extract immersion. Materials and Methods: The research design was an experimental pre-post test with control group design, n = 50 plates were divided into 2 groups, heat-polymerized acrylic resin (n=25) and thermoplastic nylon (n=25), divided into 5 groups, 3 treatment groups using 10%,15%,25% soursop leaf extract, Fittident®and aquadest in the control groups. Samples were contaminated with Candida albicans suspension and incubated, counted before immersion for 8 hours, then put in Saboroud’s bath and counted again. Results and Discussions: Wilcoxon test analysis showed significant differences in all groups, except the aquadest group. The Mann-Whitney test showed significant differences between the treatment groups, except between the 25% extract and the Fittident®control group. Also, showed a significant difference in the 15% concentration group between heat-polymerized acrylic resin and thermoplastic nylon. Conclusion:There was a difference in the decrease in the number of Candida albicans colonies on heat-polymerized acrylic resin plates and thermoplastic nylon in soursop leaf extract at a concentration of 15%, while at concentrations of 10% and 25% there was no difference

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Ekstrak bonggol nanas cayenne menghambat pertumbuhan Candida albicans pada plat resin akrilik heat curing Hump extract of cayenne pineapple inhibit the growth of Candida albicans on heat cured acrylic resin plate

2013 ◽  
Vol 12 (2) ◽  
pp. 86
Author(s):  
Prizka Brigitasari ◽  
Moh. Dharmautama
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
Negative Control ◽  
Distilled Water ◽  
Traditional Medicines ◽  
Heat Curing ◽  
Significant Difference ◽  
Protein Molecules ◽  
Complex Protein ◽  
The Media

Hump of pineapple is one of traditional medicines found in Indonesia, containing many enzyme bromelains that canparse complex protein molecules into simpler compounds. The enzyme bromelain can inhibit the growth of Candidaalbicans. The increasing of colonies of C.albicans could be due to the increased use of denture. This study shouldexplore effectiveness of hump concentration of cayenne pineapple against C.albicans growth at 30 heat curing acrylicresin plates sized 10 x 10 x 2 mm. The plates immersed in 10 ml of C.albicans and incubated for 24 hours at 37°C. Theplates were divided into 6 groups, subsequently 5 acrylic resin plates, which were soaked for 8 hours at roomtemperature in an hump extract of cayenne pineapple with the concentration of 5%, 15%, 25%, 35%, and 45%.Negative control was soaked in distilled water. 0.01 ml of a 10-3dilution and dropped into the media sabouraunddextrose agar (SDA), and then incubated for 48 hours at 37°C, and counted the number of C.albicans colonies. Analyzed with ANOVA and LSD test, it was known a significant difference of hump extract of cayenne pineapple 5%,15%, 25%, 35%, and 45% against C.albicans growth (p <0,05). On from this fact, it was concluded that hump extractof cayenne pineapple can inhibit the growth of C.albicans, which is effective at a concentration of at least 15%.

scholarly journals EFEK FUNGISTATIS, FUNGISIDAL EKSTRAK KAYU MANIS TERHADAP CANDIDA ALBICANS DAN EFEK BAKTERISTATIS BAKTERISIDAL TERHADAP STAPHYLOCOCCUS AUREUS DARI DENTURE STOMATITIS

2018 ◽  
Vol 12 (1) ◽  
pp. 15-20
Author(s):  
Minasari Minasari ◽  
Dennis Dominika
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Bactericidal Effect ◽  
Active Components ◽  
Denture Stomatitis ◽  
Cinnamon Extract ◽  
Significant Difference ◽  
Long Time ◽  
And Staphylococcus Aureus ◽  
Fungistatic Effect

Cinnamon is one of the spices that has been used for a long time, even before 2100 BC as an essential material to mummify a king's body and also as addition for foods and drinks to increase the flavours. Cinnamon has three active components such as cinnamaldehyd, eugenol and linalool. Cinnamon has good fungicidal and fungistatic effect against Candida albicans and bacteristatic, bactericidal effect against Staphylococcus aureus. Cinnamon can be used to decrease the amount of Candida albicans and Staphylococcus aureus colonies, so the researcher is interested to examine the fungistatic, fungicidal, bacteristatic dan bactericidal effect against Candida albicans and Staphylococcus aureus. The goal of this research is to determine the effect of different concentrations of cinnamon extract against Candida albicans and Staphylococcus aureus.In this research, the extract's concentrations that are used are 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, 0,195% and also two controls which are formaldehyd and aquadest. The extract used in this research is obtained by extraction method. Dilution technique is used in the test with three times replication. This research is experimental laboratorium type with pretest-postest design. Kruskal-Wallis and Mann-Whitney test is used to analyze the data.The result show that concentration 0,78 % extract has shown fungistatic effect and concentration 25% has shown fungicidal effect against Candida albicans, and concentration 1,562% bacteristatic, concentration 50% bactericidal Staphylococcus aureus. From double comparison table, we can conclude that there are significant difference between each concentration's effect against Candida albicans and Staphylococcus aureus.The result shows that the increase of cinnamon extract's concentration will lessen the remaining colonies of Candida albicans.

scholarly journals JUMLAH KOLONI CANDIDA ALBICANS PADA PLAT RESIN AKRILIK HEAT CURED SETELAH DILAKUKAN PERENDAMAN EKSTRAK DAUN KEMANGI (OCIMUM BASSILICUM LINN) 50%

2020 ◽  
Vol 16 (2) ◽  
pp. 61-63
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
Sintha Nugrahini
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Parametric Test ◽  
Group Design ◽  
Cleaning Methods

Accumulation of plaque and food scraps on an acrylic resin base will increase bacterial colonies and C. albicans which will cause denture stomatitis. Maintenance of denture hygiene using mechanical, chemical and combination of two methods. Basil leaf extract contain essential oils which are important in fight against resistant C. albicans biofilms. The purpose of this study was to determine the comparison of C. albicans colonies after cleaning various denture cleaning methods. The method of this study is used an experimental method, the study design is posttest only with control group design. Sample size of heat-cured acrylic resin plate is 40x12x3mm. this study use Kruskall-Wallis test and Mann-Whitney test as data analysis for comparison tests between groups (non-parametric test). The results showed that there were significant differences between the various cleansing methods used in reducing C. albicans colonies. The compotition of flavonoids basil leaf extract is anti-microbial which can prevent the entry of fungi that harm the body.

scholarly journals Antifungal Activity of Rosmarinus Officinalis Essential Oil and Nystatin on Store Isolate of Candida species from HIV/AIDS Patients with Oral Candidiasis

2020 ◽  
Vol 32 (3) ◽  
pp. 167
Author(s):  
Bernadya Yogatri Anjuwita ◽  
Iskandar Zulkarnain ◽  
Muhammad Yulianto Listiawan ◽  
Evy Ervianti ◽  
Rahmadewi Rahmadewi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Candida Species ◽  
Oral Candidiasis ◽  
Rosmarinus Officinalis ◽  
Minimum Fungicidal Concentration ◽  
Microdilution Method ◽  
Rosemary Essential Oil

Background: Oral candidiasis is caused by the mycotic activity of Candida albicans present in the oral cavity, and it is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/acquired immune deficiency syndrome (AIDS). The growing resistance and side effects to common antifungal drugs have promoted herbal essential oils as antifungal agents in recent years. In this study, essential oils (EO) of Rosmarinus officinalis (Lamiaceae) were examined for in vitro antifungal activ­ity against Candida species. Purpose: To evaluate the antifungal activity of essential oils of Rosmarinus officinalis (Lamiaceae) and nystatin using the microdilution technique by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of Candida spesies. Methods: This was an experimental laboratory study with a post-test-only design conducted in Dr. Soetomo General Academic Teaching Hospital, Surabaya. Forty isolates consisted of twenty isolates of Candida albicans and twenty isolates of Candida non-albicans were collected. The isolates were tested for antifungal activity using the microdilution on 96-well plates. Result: There was a significant difference from the results of the MIC concentration of rosemary essential 100% to 6.25% microdilution method between nystatin and rosemary essential oil (p < 0.05). Conclusion: The antifungal activity of rosemary essential oil was better than nystatin that the lowest MIC value, which was 6.25%, has been obtained the microdilution method. The minimum fungicidal concentration of rosemary essential oil was 25%, while the minimum fungicidal concentration nystatin was higher than 100%.

scholarly journals Effectiveness of a Chlorhexidine Digluconate 0.12% and Cetylpyridinium Chloride 0.05% Solution in eliminating Candida albicans Colonizing Dentures: A Randomized Clinical in vivo Study

2015 ◽  
Vol 16 (6) ◽  
pp. 433-436 ◽  
Author(s):  
Antoine Cassia
Keyword(s):  
Candida Albicans ◽  
Clinical Evidence ◽  
Cetylpyridinium Chloride ◽  
Test Group ◽  
Control Group ◽  
Chlorhexidine Digluconate ◽  
Denture Stomatitis ◽  
Colony Forming Units ◽  
Age Range

ABSTRACT Background Effective denture hygiene is important for patients suffering from denture stomatitis (DS). This study aimed to evaluate the efficacy of a solution containing 0.12% chlorhexidine (CHX) digluconate and 0.05% cetylpyridinium chloride (CPC) in eliminating Candida albicans colonizing dentures. Materials and methods Forty denture wearers (11 men, 29 women; age range 40 to 80 years) with clinical evidence of DS were randomly divided into two groups, one test and one control. The dentures of the test group were treated by immersion in a solution of 0.12% CHX and 0.05% CPC while those of the control group were immersed in distilled water. Swabs were collected from the fitting surfaces of the upper dentures prior and post cleaner use and examined mycologically. Results Reduction in the number of colony-forming units (CFU) of Candida albicans after immersion of the dentures in a solution of 0.12% CHX and 0.05% CPC was significantly greater than that of the control group. Conclusion A solution of 0.12% CHX and 0.05% CPC tested as a product of disinfection of the acrylic dentures showed significant results after immersion of 8 night hours for 4 days. How to cite this article Aoun G, Cassia A, Berberi A. Effectiveness of a Chlorhexidine Digluconate 0.12% and Cetylpyridinium Chloride 0.05% Solution in eliminating Candida albicans Colonizing Dentures: A Randomized Clinical in vivo Study. J Contemp Dent Pract 2015;16(6):433-436.

scholarly journals The effect of Zirconium Dioxide nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells (in vitro study)

2021 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Rudy S ◽  
Titik Ismiyati ◽  
Endang Wahyuningtyas
Keyword(s):  
Cell Viability ◽  
Zirconium Dioxide ◽  
Acrylic Resin ◽  
Zro2 Nanoparticles ◽  
Fibroblast Cells ◽  
Denture Base ◽  
Group I ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Heat cured acrylic resin is the most commonly used denture base materials.  Zirconium dioxide (ZrO2) nanoparticles can be applied as additional filler to increase mechanical strength and to reduce the amount of residual monomer. The aim of this research is to analyze the effect of ZrO2 nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells. Twenty four disc-shaped heat cured acrylic resin plate (diameter 5 mm; width 2 mm), were divided into 4 groups (n=6), they were consisted of group I control (acrylic resin), group II acrylic resin with 2.5% ZrO2, group III acrylic resin with 5% ZrO2, and group IV acrylic resin with 7.5% ZrO2. Cell viability was obtained using MTT assay and ELISA plate reader. The result is examined with one way ANOVA followed by LSD post hoc assessment. The result showed highest cell viability percentage on experimental group of 2.5% ZrO2 with value as high as 97.49%. One way ANOVA test and LSD post hoc test showed a significant difference between groups (p<0.05). The conclusion of this research is ZrO2 nanoparticles concentration utilized as filler on heat cured acrylic resin denture base is effect to viability of fibroblast cells, and ZrO2 nanoparticles 2.5% shows the highest viability of fibroblast cell compared to 5% and 7.5% ZrO2 nanoparticles concentrations  

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