scholarly journals Pulp Tissue Dissolution Capacity of Sodium Hypochlorite Combined with Cetrimide and Polypropylene Glycol

2013 ◽  
Vol 24 (5) ◽  
pp. 477-481 ◽  
Author(s):  
Luiza Helena Silva de Almeida ◽  
Natália Gomes e Silva Leonardo ◽  
Ana Paula Neutzling Gomes ◽  
Luciano Giardino ◽  
Erick Miranda Souza ◽  
...  

This study evaluated the influence of the addition of cetrimide and polypropylene glycol to sodium hypochlorite (NaOCl) on its capacity to dissolve pulp tissue. Bovine pulp fragments with standardized weight and volume were immersed for 5, 15 and 30 min in 2 mL of NaOCl and Hypoclean (NaOCl added with cetrimide and polypropylene glycol) solutions at 5.25%, 2.5%, 1%, 0.5% and 0.25% and afterwards re-weighted. Distilled water was used as a control. The percentage of tissue loss was considered for statistical analysis (univariate ANOVA, SPSS, v. 17.0) at 5% significance level. There was no tissue dissolution in the control group. NaOCl added with surfactants (Hypoclean) dissolved more pulp tissue (p<0.05) than NaOCl alone. Tissue dissolution was directly dependent on the concentration of solutions (p<0.05), and also on the time range (p<0.05). The combination of NaOCl at high and low concentrations with the surfactants cetrimide and polypropylene glycol increased significantly its capacity to dissolve pulp tissue.

2018 ◽  
Vol 10 (1) ◽  
pp. 13-19
Author(s):  
Kiran Kumar N ◽  
Biji Brigit K ◽  
Savitha B Naik ◽  
Seema Merwade

BACKGROUND: The cytotoxicity of Sodium hypochlorite has always been a concern when used as an endodontic irrigant. Quest for an equally effective, but less toxic substitute has led to researches on Calcium hypochlorite (Ca(ClO)2 ) solutions. But it has been proven that 10% Calcium hypochlorite solution at room temperature (250 C) demonstrated slower rate of tissue dissolution than Sodium hypochlorite. Aim: This study was performed to assess if preheating enhanced the tissue dissolution capacity of 10% Ca(ClO)2 . MATERIALS AND METHODS: Human pulp tissue was obtained from freshly extracted single rooted mandibular premolars. The tissue weight was standardized to 9.5mg. The test groups consisted of freshly prepared Ca(ClO)2 solutions at room temperature (250 C), 450 C and 600 C. Ten specimens were assigned to each group with each specimen immersed in 2ml of the test solution. Five specimens immersed in normal saline made the control group. Every five minutes the tissue was retrieved, blotted dry, weighed and immersed back in fresh 2 ml of solution. This was continued for 60 minutes or till complete dissolution. The percentage difference in weight at each interval was calculated. The values obtained were statistically analyzed using one –way ANOVA. Intergroup comparison at different time intervals was done using Bonferroni test. RESULTS: Ca(ClO)2 solution at 600 C dissolved the tissue significantly faster than other solutions. The tissue dissolution rate of solution at 450 C was greater than that at 250 C. (P< 0.05) CONCLUSION: Within the limitations of this study, it can be concluded that preheating 10% Ca(ClO)2 solution significantly enhanced its tissue dissolution capacity.


Author(s):  
Alfredo Iandolo ◽  
Alberto Dagna ◽  
Riccardo Beltrami ◽  
Claudio Poggio ◽  
Mariano Malvano ◽  
...  

INTRODUCTION The goal of root canal treatment is to shape and clean the endodontic space, reducing the bacterial load and removing the pulp tissue. Obviously, the action of the endodontic instruments is limited to the main canals, regardless of the complexity of the endodontic space. Consequently, finding the best possible cleaning technique, which can be obtained chemically using irrigation solutions, is a fundamental aid in the endodontic therapy. One of the most commonly used root canal irrigant is sodium hypochlorite (NaOCl), available in various commercial formulations. The effectiveness of NaOCl is undeniable. However, the action of dissolution of the pulp tissue is merely dependent on the concentration and the characteristics of the irrigant itself. AIM The aim of this study is to evaluate the effective concentration of different commercial formulas of sodium hypochlorite, by evaluating the percentage of total chlorine in each product. The dissolution capacity of the pulp tissue of each of the tested products was then analyzed by measuring the required time. MATERIALS AND METHODS Three commercial types of sodium hypochlorite were selected for this study: 5% NaOCl (ACE, Procter &amp; Gamble), 5% NaOCl (N5, Simit Dental) and 6% NaOCl (CanalPro, Coltene). For each product, 10 packages were used, from which samples of the product were taken and 30 x 5 ml tubes were filled. All samples were divided into 3 groups and were analyzed using the DIN EN ISO 7393-2 method and the percentage of total chlorine (expressed as a percentage) was calculated. 40 samples of vital pulp were obtained from teeth freshly extracted for periodontal reasons and stored in physiological solution. In order to unify the size and weight of the samples (0.0001 mg), a microtome and a precision balance (Pro Explorer Ohaus) were used. Each sample, carefully examined by stereomicroscope (40x), was placed in artificial plastic containers and submerged in 0.1 ml of irrigating solution at room temperature (26 &deg; C). A fourth control group used saline solution as irrigant. Simultaneously with the insertion of the irrigating solution, a digital stopwatch was activated and the time necessary for the complete dissolution of the pulp sample was measured. The data obtained were subjected to statistical analysis. RESULTS The average percentages of chlorine detected for each group were: 4.26% (ACE), 5.16% (N5) and 5.97% (CanalPro). The Kruskal-Wallis test showed statistically significant differences between the different commercial formulations of hypochlorite (P &lt;0.05). CanalPro showed the lowest values, while ACE showed the highest values of dissolution time of the pulp. DISCUSSION The analysis of the total chlorine percentage found that the actual concentration of the sodium hypochlorite in the samples is close to the values declared by the manufacturers both in the case of N5 and CanalPro. On the contrary, the concentration detected in the samples of common bench bleach (ACE) is significantly lower, which has average values less than 5%. This explains the longer time taken for the complete dissolution of the pulp tissue. The average dissolution time of the pulp samples was in fact inversely proportional to the concentration detected in the tested irrigants, so that a lower time corresponds to a higher concentration.


2011 ◽  
Vol 12 (4) ◽  
pp. 252-258 ◽  
Author(s):  
Cintia Iara Oda Carvalhal ◽  
José Antônio Nunes de Mello ◽  
Lourenço Correr Sobrinho ◽  
Américo Bertolazzo Correr ◽  
Mário Alexandre Coelho Sinhoreti

ABSTRACT Aim The aim of this study was to evaluate the influence of immersion period in two disinfectant solutions on dimensional change of four elastomeric impression materials. Materials and methods Four representative materials of each class of elastomers: Xantopren (polydimethylsiloxane—PDS), Express (polyvinyl siloxane—PVS); Permlastic (polysulfide— PS) and Soft Impregum (polyether—EP) were mixed according to manufacturers’ instructions and then inserted into a metal matrix prepared according to the specification of ISO 4823. The molds were removed from the matrix after 7 minutes and immersed in disinfectant solutions (sodium hypochlorite 0.5 and 2% glutaraldehyde) for 5, 10, 20, 30 and 60 minutes (n = 7), except the control group, which was not immersed. Once removed from solutions, the test samples were washed in water for 15 seconds, dried and measured three times, using a comparative optical microscope, with accuracy of 0.0005 mm. The analysis of variance with three criteria and Tukey's test with significance level of 5% showed that differences in linear dimensions of the materials analyzed were not statistically significant (p > 0.05) after soaking in sodium hypochlorite 0.5 and 2% glutaraldehyde, for the tested periods of time. Clinical significance Combinations of molding material and disinfecting solution can be used in the dental clinic for infection control, without changing the dimensional molds, for immersion periods not longer than 20 minutes. How to cite this article Carvalhal CIO, de Mello JAN, Sobrinho LC, Correr AB, Sinhoreti MAC. Dimensional Change of Elastomeric Materials after Immersion in Disinfectant Solutions for Different Times. J Contemp Dent Pract 2011;12(4): 252-258.


2015 ◽  
Vol 26 (5) ◽  
pp. 537-540 ◽  
Author(s):  
Renata Senger Niewierowski ◽  
Luíza Rodrigues Scalzilli ◽  
Renata Dornelles Morgental ◽  
José Antônio Poli de Figueiredo ◽  
Fabiana Vieira Vier-Pelisser ◽  
...  

Abstract: The aim of this study was to evaluate the tissue dissolution ability of different irrigating solutions associated or not to ultrasonic agitation. Ninety bovine pulp fragments (n=10 per group) were weighed and then placed individually in Eppendorf test tubes containing the following irrigants: G1- 2.5% sodium hypochlorite (NaOCl); G2- 2.5% NaOCl + ultrasonic agitation (US); G3- 2.5% NaOCl + 0.2% cetrimide (CTR); G4- 2.5% NaOCl + 0.2% CTR + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- 0.2% CTR; G8- 0.2% CTR + US; G9- saline solution. Two blinded observers assessed the samples continuously for the first 4 h, and then every hour for the next 12 h. Dissolution speed was calculated by dividing the initial pulp weight (mg) by the period of time until complete dissolution (min). Data were compared by ANOVA and Tukey post hoc test with a 5% significance level. G1 to G4 dissolved pulp fragments completely and G2 was significantly faster than the other groups. G5 to G9 did not exhibit dissolving activity. In conclusion, only groups containing NaOCl were capable of pulp tissue dissolution, which was enhanced by ultrasonic agitation, but did not alter when 0.2% cetrimide was associated. This isolated solution and Sterilox showed no dissolving capacity, regardless the use of ultrasound.


2016 ◽  
Vol 15 (1) ◽  
pp. 70
Author(s):  
Kiany Scarssi Nunes ◽  
Letícia Feron ◽  
Francisco Montagner ◽  
Tiago André Fontoura de Melo

Aim: To analyze the root canal organic tissue dissolution capacity promoted by irrigating solutions, with or without the use of different agitation techniques. Methods: Bovine pulp tissue fragments were initially weighed. The following irrigating solutions were tested: 2.5% sodium hypochlorite, 2% chlorhexidine digluconate solution, and distilled water. The irrigating protocols were: immersion, mechanical agitation with endodontic files, and ultrasonic or sonic systems (Endoactivactor® and Easy Clean®). At the end of the protocols, the pulps were weighed to determine their final weight. For comparison, the average percentage of tissue dissolution in relation to the groups was analyzed using the Kruskal-Wallis nonparametric test complemented by multiple comparisons test. The significance level was set at 5%. Results: Among the irrigation solutions, 2.5% sodium hypochlorite showed a higher dissolving power than 2% chlorhexidine digluconate and distilled water. Furthermore, ultrasonic and sonic systems were more effective irrigating protocols than immersion and mechanical agitation with endodontic files. Conclusions: The combination of sodium hypochlorite with an agitation system promotes a greater degree of tissue degradation.


2017 ◽  
Vol 4 (1) ◽  
pp. 12
Author(s):  
Dessy Lutfiasari ◽  
Mahmudah Mahmudah

The use of the current method of learning very big influence on the growth and development of students' creativity and interest for all subjects to be taught, especially in the use of partograf. From interviews to the 10 students of IV semester Prodi Midwifery (D-III) Kadiri University is known that 4 (40%) of students said it was understood, 4 (40%) of other students say they are confused and 2 (20%) of them said that he was a student not familiar with partograf. This shows the lack of understanding of students in filling partograph. The research objective is to determine the effectiveness of the use of learning methods for skills training simulation with filling partograph the second semester students in Midwifery (D-III) Kadiri University Faculty of Health Sciences in 2015. The research design used is pre experiment with design Static Group Comparison/Posttest Only Control Group Design. The population studied were all students of the second semester in Midwifery (D-III) Faculty of Health Sciences University of Kadiri numbered 50 students and sampling techniques Federer totaled 32 students. This is a research instrument partograph sheet. Results of the study were analyzed using the Mann Whitney test with a significance level of 0.05 were used.The results showed 7 respondents (46.7%) are adept at using partograf with simulation teaching methods and 6 respondents (40.0%) are adept at using partograph with practice learning methods. Data were analyzed by Mann Whitney test obtained ρ = 0.965; α = 0.05 means that H0 is accepted and H1 rejected. This means there is no difference in the effective use of learning methods for skills training simulation with partograph filling. Based on the results of this study are expected to choose the method of learning as a learning method in charging partograph because both methods equally effective.; Keywords: simulation methods, drilling methods, partograph filling


Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde


2021 ◽  
Vol 9 (1) ◽  
pp. 20-31
Author(s):  
Hasan Huseyin Aksu ◽  

The purpose of this study is to answer if there is a reasonable difference on academical success of students who get education with traditional and RME approach question on “Teaching geometrical objects to 8th grade students” subject. Study group consists of 47 students which contains 21 experimental and 16 control group from “Ordu Anadolu İmam Hatip High School Project School” in Altinordu, Ordu. Experimental and control group have same academical success level, as the school which this study has runned is a school which accepts students with an exam only. After the experimental and control groups were created, a 25 question pre-test was performed to understand the level of knowledge of the group regarding geometrical objects. The same test was performed on the same groups 8 weeks later as retention test. To determine opinions of the students in experimental group regarding RME and related learning activities, semi-structured interviews are conducted. The data obtained from the pretest, posttest and retention tests were analyzed with t-test for independent samples and t-test for dependent samples and variance analysis for mixed measurements with 0.05 significance level. According to the results, it is seen that learning activities prepared according to RME approach are much more effective than learning activities prepared according to the traditional approach on students’ academic success.


2018 ◽  
Vol 1 (3) ◽  
pp. 26-37
Author(s):  
Rana Wijaya ◽  
M.Ihsan Dacholfany

Reading is one of the important aspects of language learning. By reading, the learners get knowledge and information. But in reality, students are often ignoring to mastering this skill. It is the researchers found in the second grade of SENIOR HIGH SCHOOL Kartikatama Metro Lampung Indonesia, where students have difficulty in reading comprehension, so this leads to lower their learning. Therefore, to improve students' reading comprehension ability, researchers used Written Retelling Strategy (WRS) in the learning process.        The purpose of this study was to determine whether the use of Retelling Written Strategy (WRS) has a positive effect on students' reading comprehension in learning English. Written Retelling Strategy (WRS) considered as one strategy that makes students more active and fun in the learning process to comprehend some text.        Forms of this research are Quantitative Research conducted in two classes. Each class is divided into the trial and reserve grade. Author implements True Experimental Design (Pure Research) by applying a pre-test and post-test as an instrument. In accordance with the method of this study, the data were analyzed using t-test that allows knowing the difference between the experimental group that used a class of written retelling strategy and the control group who did not use written retelling strategy. Finally, the research data show that t observations = 3,590 and is included in the category of high impact. Furthermore, t observation T Table consult with a significance level of 5% = 2.010 and 1% = 2,682, the data states that t observations greater than the T Table, this could mean that Hi is received. And could mean that there is a positive and significant influence between experiment class and class control on the use of written retelling strategy at second grade of Senior High School  Kartikatama Metro.


2017 ◽  
Vol 68 (2) ◽  
pp. 323-327
Author(s):  
Cristian Levente Giuroiu ◽  
Maria Vataman ◽  
Gabriel Melian ◽  
Dragos Bularda ◽  
Ludmila Lozneanu ◽  
...  

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.


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