scholarly journals The long-term administration of a clinoptilolite-supplemented feed to layers and its effect on performance, haematological parameters and metabolic profile

2008 ◽  
Vol 53 (No. 5) ◽  
pp. 212-218 ◽  
Author(s):  
E. Straková ◽  
P. Suchý ◽  
I. Herzig ◽  
V. Šerman ◽  
N. Mas

120 selected layers (Bovans Goldline hybrid) aged 19 weeks with an average weight of 1 735 g were divided into two balanced groups: control group (Group C) and experimental (Group E). Layers were reared in three-tier cages with automatic watering, manual feeding, and at controlled light and temperature regimens. One cage accommodated two layers, the floor surface area was 0.1125 m<sup>2</sup> per layer. The experiment started after a 20-day adaptation period with layers aged 22 weeks (Week 22) and ended when layers were 68 weeks old (Week 68). In a period of initiation (i.e. Weeks 19–38), layers were fed the complete feeding mixture N1. Then they received the feeding mixture N2 until the end of the experiment. Feeding mixtures in both groups (C and E) had the same composition; the only difference between mixtures was that the feeding mixture in the experimental group was enriched with 1% clinoptilolite (i.e. the commercially available additive ZeoFeed). Layers received feeding mixtures and drinking water <I>ad libitum.</I> In the course of the experimental period, control layers laid 16 289 eggs while experimental layers laid 16 474 eggs. It follows from the results that the laying intensity in experimental layers was 1.7% higher as compared to control layers, i.e. the number of laid eggs in experimental layers increased by 5.6 eggs per layer. The mean weight of all laid eggs was 66.3 ± 6.25 g in the control group and 65.6 ± 5.44 g in the experimental group (<I>P</I> ≤ 0.01). Such performance was achieved at the consumption of feeding mixture being 141.7 g per laid egg in the control group and 137.6 g per laid egg in the experimental group. The consumption of feeding mixture in the experimental group was 4.1 g lower than that in the control group. The mean values of parameters monitored in blood plasma such as uric acid, cholesterol, glucose, lactose, calcium, phosphorus, ALP, and LDH in both groups of layers ranged within reference intervals, with no significant differences being detected between both groups.However, statistically significant differences between both groups were found in total plasma protein (<I>P</I> ≤ 0.01), triacylglycerol levels (<I>P</I> ≤ 0.05), and magnesium (<I>P</I> ≤ 0.01), which were elevated in the control group, and in AST (<I>P</I> ≤ 0.05) whose level in the control group was significantly lower than that in the experimental group. The results of haematological tests performed with layers’ blood revealed statistically significant changes in parameters such as the erythrocyte count (<I>P</I> ≤ 0.01), haemoglobin level (<I>P</I> ≤ 0.01), and MCHC (<I>P</I> ≤ 0.05), which were elevated in the experimental group, and in the leukocyte count (<I>P</I> ≤ 0.05), which was lower in the experimental group, as compared with the control. However, the values found varied within physiological ranges.

2015 ◽  
Vol 36 (5) ◽  
pp. 3329 ◽  
Author(s):  
Leison Rocha Bezerra ◽  
Ricardo Loiola Edvan ◽  
Ronaldo Lopes Oliveira ◽  
Aderbal Marcos de Azevedo Silva ◽  
Geraldo Fábio Viana Bayão ◽  
...  

<p>This paper aims to evaluate the influence of different levels of supplemental product-dehydrated cashew in the feed for crossbred Girolando cows on milk production and hemato-biochemical profile. The experiments were conducted using eight cows that were randomly distributed in a Latin square design (4×4), in which treatments consisted of four supplementation levels with product-dehydrated cashew (PDC) in animal diets, namely: 0% PDC (control diet), 1.0 kg of DCP, 1.5 kg of PDC and 2.0 kg of PDC in total dry matter . The milk and animals were weighed and the body condition of the animals were evaluated within seven days of milk collection during each experimental period. To determine the serum biochemistry and blood count of cows, three blood samples were taken in each period, at 7, 14 and 21 days before the daily supply of supplementation with samples collected from 5 ml of blood by puncturing the jugular vein. Supplementation with 2 kg of PDC reduced milk production and the levels of glucose and total plasma protein compared to the control group, due to the higher level of tannin in this group. Supplementation with PDC raised the total count of the erythrocytes, reduced the mean corpuscular volume (MCV) and non-changed number of leukocytes. Increased levels of phenolic compounds in the diet inhibited the absorption of dietary proteins, which decreased milk production.</p>


2014 ◽  
Vol 71 (5) ◽  
pp. 491-498 ◽  
Author(s):  
Vlado Cvijanovic ◽  
Danilo Vojvodic ◽  
Dragan Djurdjevic ◽  
Milena Jovic ◽  
Vojkan Stanic ◽  
...  

Bacgraund/Aim. The use of new therapeutic methods to prevent development of fibrothorax as the final complication of the human pleural infections requires research with experimental animals. The aim of this study was to standardize the procedures for the establishment of our own experimental model of empyema in rabbits, since it should be able to offer similar conditions found in human pleural infections. Methods. This experiment included 15 chinchilla rabbits, weighing from 2.3 to 2.8 kg. There were 12 rabbits in the experimental group, while 3 rabbits formed the control group. On the first day, we administered 0.4-0.5 mL of turpentine in the right pleural space of the rabbits from the experimental group in order to provoke sterile exudative pleurisy. After 24 h we injected 1 mL of Staphylococcus aureus and 1 mL of Escherichia coli bacteria in the same concentration of 4.5 x 108 bacteria/mL. Thoracocentesis for the pleural fluid analysis was performed 24, 48, 72, and 96 h after bacteria instillation. In these pleural samples we estimated the number of leucocytes and the values of lactate dehydrogenase (LDH), glucose and pH in pleural fluid, as well as the presence of bacteria. We did not protect the animals with antibiotics, and on the day 7 of the experiment they were sacrificed with the lethal dose of barbiturate (iv). The lung from the empyemic side of all experimental animals and the lung of one control animal were histopathologically examined. Results. A total of 4 animals had a small amount of clear pleural fluids or there was no fluid obtained with thoracocentesis 24 and 48 h after the bacteria instillation. after the bacteria instillation. In the remaining 8 rabbits 24 h after bacteria administration the mean values (? SD) of the parameters monitored were as follows: Le 34.75 ? 6.13 x 109/L, LDH 17,000 ? 4,69 U/L, glucose 1.23 ? 0.45 mmol/L, and pH 6.975 ? 0.15. The obtained values met the criteria for the evaluation of effusion as pleural empyema or complex and complicated pleural effusion (LDH > 1000 U/L, glucose < 2.31 mmol/L and pH < 7.20). Bacterial cultures were positive in 5 out of 8 first pleural samples and in only 2 samples after 48 h of bacteria administration. There was a positive correlation between the number of leukocytes and the LDH value (r = 0.071, p < 0.001), and a negative correlation between the number of leukocytes and the glucose level (r = 0.864, p < 0.001), and the leukocytes number and pH of the pleural fluid (r = 0.894, p < 0.001). The mean glucose value increased after 48 h (3.23 ? 0.44 mmol/L), and the pH value rose after 72 h (7.22 ? 0.03) which was beyond the empyema level. Conclusion. The creation of the experimental empyema model is a very delicate work with uncertain success. Its value and importance are crucial for pleural pathology research. With the intention to obtain a more empyemic pleural reaction we created a model with two different human pathogen bacteria. We generated the satisfactory results, but not as good as those contained in some of the reference literature data.


2019 ◽  
Vol 90 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Kadir Kolcuoğlu ◽  
Aslihan Zeynep Oz

ABSTRACT Objective To evaluate the difference in orthodontic root resorption between root-filled and vital teeth. Material and Methods Sixteen individuals who required bilateral premolar tooth extraction due to orthodontic treatment and had a previously root-filled premolar tooth on one side were included in the study. The experimental group consisted of root-filled premolar teeth, and the control group consisted of contralateral vital premolar teeth. A 150-g buccally directed force was applied to these teeth using 0.017 × 0.025-inch TMA cantilever springs. The premolars were extracted 8 weeks after the application of force. Images were obtained using micro–computed tomography. Resorption measurements were obtained using the Image J program. Results The mean values for resorption were 0.08869 mm3 for the root-filled teeth and 0.14077 mm3 for the contralateral teeth, indicating significantly less resorption for the root-filled teeth compared with the contralateral teeth after the application of orthodontic force (P = .003). In both groups, the most resorption was seen on the cervical-buccal and apical-lingual surfaces. The mean resorption value of the cervical region was 0.06305 mm3 in the control group and 0.0291 mm3 in the experimental group, and the difference was statistically significant (P = .002). Conclusions Root-filled teeth showed significantly less orthodontic root resorption than vital teeth.


2021 ◽  
Vol 8 (1) ◽  
pp. 037-042
Author(s):  
Emmanuel TonbraEgoro ◽  
Ikhide Godwin Ilegbedion ◽  
Prudence NkemdinimOkara

This study was aimed on the biochemical and histomorphological study of abuse in the consumption of alabukun powder in Rattus norvegicus rats. Five milliliter of blood specimen was collected into lithium heparin bottles from seven rats weighing 240±2g respectively with each of them administered with 0.2mg/kg alabukun powder daily for a period of two weeks (experimental group) while another seven rats weighing 240±2g each were not administered with alabukun powder (control group). Thereafter alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein were measured quantitatively in both groups of rats. The mean values of all the measured biochemical parameters in Rattus norvegicus rats in the experimental group were statistically significant (p<0.05) as compared to that in the control group. This established biochemical finding was in conformity with the histomorphological examination of the kidney and liver organs. In conclusion, this study has established that administration of 0.2mg/kg of alabukun powder on daily basis for a period of two weeks on Rattus norvegicus rats may induce hepato-renal and inflammatory disorders. It is thus recommended that consumption of alabukun powder by humans should strictly be in compliance with its prescription. However, kidney, liver and inflammatory biomarkers should be quantitatively measured in humans that have abused its consumption with a view to ascertain their health status and prevent any deleterious risks.


2017 ◽  
Vol 13 (12) ◽  
pp. 24
Author(s):  
Ricardo Zambrano Moreira ◽  
Juan Carlos Gómez Villalva ◽  
Johns Rodríguez Álava ◽  
Hugo Alvarado Álvarez ◽  
Luis Quezada Gallardo ◽  
...  

The objective of this research was to evaluate different levels of mannans oligosaccharides (MOs) in productive parameters and intestinal health in broilers. Two hundred and forty one-day-old broiler chickens (Ross 308 line) were used during a 6-week experimental period. A completely randomized design with four treatments and six replicates was used. The evaluated levels were 0.0 kg t-1 (T0); 0.5 kg t-1 (T 1); 1 kg t-1 (T2) and 1.5 kg t-1 (T3). For the mean comparisons, a Tukey test (P≤0.05) was used. The source of oligosaccharide mannans derived from the cell wall of Saccharomyces cerevisiae and it was added to the commercial balanced feed. Productive parameters, intestinal villi and economic performance (costbenefit) were evaluated. The data was analyzed with the statistical program SAS V9 (2012). At 42 days of the test, T2 reached the highest average weight of 2455.48 g. For feed consumption, the mean average for T0, T1, T3 and T2 were 4342.63 g, 4304.5 g, 4254.32 g and 4216.80 g respectively. T2 had higher weight gain compare to the control group, 535.70 and 388.84 g, respectively. Feed conversion was higher for T2 (1.71) compared to the control group (1.85). T1 obtained had a carcass yield of 75.89%. Mortality did not show significant differences (P≥ 0.05). Length and width (μm) of the intestinal villi were different (P≤0.05) among treatments, at 21 days of, T3 showed the longest (1939.74 μm) and widest (326.03 μm) measures at the duodenum site. The economic analysis, all the treatments were profitable results, although T1 had the highest cost-benefits ($ 1.30).


2021 ◽  
Vol 16 (3) ◽  
pp. 103-108
Author(s):  
Emmanuel Tonbra Egoro ◽  
Emmanuel Sunday Oni ◽  
Otaraku Jonathan Oye ◽  
Annabel Awele Idama

This study was aimed on assessment of selected biochemical parameters among food hawkers along the streets of Yenagoa, Bayelsa State, Nigeria. Five milliliter of blood specimen was collected from fifteen food hawkers with ≤ 5 years working experience (experimental group one), ≥ 5 years working experience (experimental group two) and nonfood hawkers (control group) respectively into lithium heparin anti-coagulated bottles. Thereafter alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein were measured quantitatively. The mean values of volunteers in experimental group one showed no statistically significant difference (p>0.05) in alanine aminotransferase (7.46 ± 1.02), aspartate aminotransferase (7.02 ± 0.86), urea (7.70 ± 1.29) and creatinine (70.44 ± 3.54) as compared with the control group (7.42 ± 0.98), (6.98 ± 0.82), (7.65 ± 1.28) and (70.40 ± 3.52) respectively, but there was a statistically significant difference (p<0.05) in C-reactive protein (17.40 ± 1.98) when compared with the control group (2.70 ± 0.70). However, the mean values of food hawkers in experimental group two showed statistically significant difference (p< 0.05) in all the measured biochemical parameters 27.70±2.98, 21.40±2.06, 15.50±2.02, 110.70±3.74, 29.42±3.20 as compared with that of the control group 7.42±0.98, 6.98±0.82, 7.65±1.28, 70.40±3.52, 2.74±0.70. The volunteers in experimental groups one and two showed 13% -27% and 53% -80% abnormal values of the measured biochemical parameters respectively as compared with that of the control group. In conclusion, alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein may be altered in food hawkers with ≥ 5 years working experience. It is therefore recommended that food hawkers in this category should go for hepato-renal and inflammatory biochemical parameters checkup occasionally in any registered and licensed Medical Laboratory Facility.


2018 ◽  
Vol 11 (6) ◽  
pp. 1 ◽  
Author(s):  
Ersin Nacaroglu ◽  
Onder Karakoc

This is an experimental study and it was aimed to identify the effects of 8-week plyometric training of hearing-impaired athletes on balance. 24 Male hearing-impaired volleyball players participated in the study. Random method was used when experimental and control groups were identified. Subjects used in the study were divided into two groups as experiment (12 male age = 19.08 ± 1.62) and control group (12 male age = 17.75 ± 1.28). 3 days/90 min. volleyball training programme was applied for two groups per week.For the experimental group, plyometric training was applied after warming before the volleyball training sessions. The control group continued their regular volleyball training. Statistical results were evaluated at 95% confidence interval and p <0.05 significance level. Because the pre-test and post-test measurements of the groups were normal and homogeneous distribution, the Paired Samples T-Test was applied for the significance between them. To analyze the differences between the experimental group and control group, Independent Samples T-test was used.As a result of the plyometric exercises, the mean values of the pre-test and post-test differences of the static balance measurement parameters for the experimental and control groups were found to be significant in the values of overall stability R, medial lateral R, and overall stability L values (p <0.05). But, in the mean values, there was no significance of anterior posterior R., anterior posterior L. and medial lateral L. mean (p> 0.05).Consequently, it can be said that plyometric exercises have a positive effect especially on static balances of hearing-impaired volleyball players.


2008 ◽  
Vol 53 (No. 1) ◽  
pp. 31-35 ◽  
Author(s):  
P. Suchý ◽  
E. Straková ◽  
F. Vitula

The main aim of the study was to assess the effect of a diet supplemented with L-carnitine on egg weight and laying rate in hens of pheasant (<I>Phasianus colchicus</I>). The experiment lasted 14 weeks and was performed with 210 experimental and 210 control laying hens. The birds were kept in cages; one cock and seven hens per cage. Both control and experimental hens were administered the complete feed mixture, with the only difference that the feed mixture administered to experimental hens was supplemented with L-carnitine at a level of 0.01%. During the experimental period, eggs were collected and weight of individual eggs was determined. Control and experimental groups provided 8 039 and 8 499 eggs, respectively. The results showed that L-carnitine increased egg weight (<I>P</I> < 0.01) and laying rate. The effect of L-carnitine on egg weight and laying rate manifested itself until weeks 11 and 13, respectively. The average weight of eggs laid during the experimental period was (mean ± SD) 32.22 ± 3.03 g in the control group and 32.51 ± 2.83 g in the experimental group, with the overall laying rate being 44.29% in the control group and 47.30% in the experimental group.


Author(s):  
J. Santoantonio ◽  
L. Yazigi ◽  
E. I. Sato

The purpose of this study was to investigate the personality characteristics in adolescents with SLE. The research design is a case-control study by means of the Rorschach Method and the Wechsler Intelligence Scale. Study group: 30 female adolescents with lupus, 12–17 years of age. The SLE Disease Activity Index was administered during the period of psychological evaluation. Control group: 32 nonpatient adolescents were matched for age, sex, and socioeconomic level. In the Wechsler Intelligence Scale the mean IQ of the experimental group was significantly lower than that of the control group (77 and 98, respectively, p < .001). In the Rorschach, the lupus patients showed greater difficulty in interpersonal interactions, although they displayed the resources to process affect and to cope with stressful situations. A positive moderate correlation (p = .069) between the activity index of the disease and the affect constriction proportion of the Rorschach was observed: the higher the SLEDAI score, the lower the capacity to process affect. There is a negative correlation between the activity index of the disease and the IQ (p = .001): with a higher activity index of the disease, less intellectual resources are available.


Author(s):  
Ashraf Albrakati

Tramadol, a broadly in recent years, is an effective analgesic agent for the treatment of moderate to acute pain. Its metabolites are excreted by the kidney which may cause nephrotoxicity. Moringa oleifera leaves are commonly used to provide herbal and plant-derived medicinal products especially in developing nations. The present study was carried out to determine the biochemical and histopathological changes in the kidney of tramadol-treated albino mice and to evaluate the possible protective role of Moringa oleifera leaves against tramadol-induced nephrotoxicity. Twenty adult albino mice were divided into four groups. Control group (group i) received daily intraperitoneal injection of normal saline only, group ii received oral dose of Moringa oleifera leaves extract (20 mg/kg/bw) for three weeks, group iii received daily intraperitoneal dose of tramadol (0.3 mg/kg/bw) for the same period, group iv, received daily oral dose of Moringa oleifera leaves extract, (20 mg/kg/bw) three hours before injecting intraperitoneal dose of tramadol (0.3 mg/kg/bw), for the same period. Blood samples were withdrawn at the end of the experiment for kidney function tests and specimens from the kidney were processed for histological study. No significant differences in the mean values of the kidney function tests were noticed between Moringa oleifera group and control group. However, there was highly significant increase in the mean values of serum, urea and creatinine in tramadol-treated group as compared to the control group. Although tramadol + Moringa oleifera group revealed significant difference in the mean values of urea and creatinine when compared with tramadol-treated group. So, Moringa oleifera leaves extract have been shown to attenuate the renal dysfunction, improve the renal architecture, with nearly normalization of serum urea and creatinine levels which indicate improvement of renal function. In conclusion, in the light of biochemical results and histological findings, co-administration of Moringa oleifera leaves lessened the negative effects of tramadol-induced nephrotoxicity; possibly by its antioxidant action. Further investigation of these promising protective effects of Moringa oleifera leaves against tramadol-induced renal injury may have considerable impact on developing an adjunct therapy aiming to improve the therapeutic index of some nephrotoxic drugs.


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