scholarly journals THE EFFECT OF SALTWATER FISH NANOPARTICLE POWDER CONSUMPTION ON TOOTH ENAMEL DENSITY In Vivo Study of Mice (Mus musculus)

2021 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Sandy Christiono ◽  
Fera Putri Ardiani ◽  
Welly Anggarani ◽  
Fourier Dzar Eljabbar

ABSTRACTBackground: Dental caries is rarely found in children who eat saltwater fish. Overfished fish can be used as powder of saltwater fish nanoparticles, which is useful as an alternative material for the prevention of dental caries in dentistry can be used as a powder for saltwater fish nanoparticles, which is useful as an alternative material for preventing dental caries in dentistry. A Cone Beam Computed Tomography (CBCT), commonly known as a μ-CT Scanner, is a device used to quantify the increase in tooth enamel density Purpose: This study aimed to determine the effectiveness of saltwater fish nanoparticle powder consumption on tooth enamel density of Mus musculus. Method This study used an experimental research method with a randomized posttest only control group design. The research subjects were 16 mice taken from 2 pregnant female mice which were divided into two groups, namely the treatment group with 2.17 mg / 0.5 mL of saltwater fish nanoparticle powder and the control group which was given distilled water. Administration was carried out during the intrauterine period until the teeth of the mice grew, then observed the tooth enamel density with CBCT OP 3D Pro (KaVo, Germany). The data obtained were analyzed by Levene and continued with the Independent T-test. Result: The average value of enamel density in mandibular incisors in the treatment group was greater than the control group (p <0.05). Conclusion: Saltwater fish nanoparticle powder can increase tooth enamel density of Mus musculus. Keywords: CBCT, CT nanoparticle powder, saltwater fish, tooth enamel density,

2020 ◽  
Vol 10 (2) ◽  
pp. 48
Author(s):  
Sri Kunarti ◽  
Aulia Ramadhani ◽  
Laskmiari Setyowati

Background: Dental caries is one of the most common infectious diseases and often occurs in the community caused by bacteria. Attached bacteria in the tooth surface for a long time will form a biofilm and will lead to demineralization characterized by damage in the structure of the tooth enamel. The bacteria that cause dental caries and can form biofilms is Streptococcus mutans. The bacteria inside biofilms are more resistant to antibacterial agents. Flavonoids in mangosteen pericarp extract can be a cleaner alternative for the anti-biofilm cavity that has properties against Streptococcus mutans. Purpose: To determine the activity of flavonoids in mangosteen pericarp extract at a certain concentration against Streptococcus mutans bacteria. Methods: This study was a laboratory experimental study with a post-test only control group design. Streptococcus mutans were diluted according to the Mc Farland dilution standard 106 in Tryptic Soy Broth (TSB) medium and put in a flexible U-bottom microtiter plate. Then it was incubated for 5x24 hours and checked using crystal violet simple staining to see the formation of biofilms. Flavonoid extract of mangosteen pericarp performed serial dilution in a concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% was added, and the incubation process were conducted for 1x24 hours. OD (Optical Density) readings were done with a wavelength of 595 nm. Results: There was a significant difference between the test groups and the positive control group. The concentration of 100% had the anti-biofilm activity and showed the value of the highest percentage of inhibition, whilst the concentration of 0.78% showed a minimum biofilm inhibition concentration. The results were demonstrated by a statistical analysis test. Conclusion: Flavonoid extract of mangosteen pericarp at a certain concentration has anti-biofilm activity against Streptococcus mutans biofilm.


Author(s):  
Khaerul Anam ◽  
I P G Adiatmika ◽  
I Putu Adiartha Griadhi ◽  
I Made Muliarta ◽  
Luh Putu Ratna Sundari ◽  
...  

Boxing is the basic gesture for all martial arts, because all of them definitely use the shadow shot and common shot. These two types of shots are related each other because anaerobic capacity must go along with aerobic capacity.To attain the proper knowledge about the method of shadow shot and common shot in increasing VO2max for the endurance of arm muscles for boxer athletes in Central Lombok Regency. Research was experimental with pre-test and post-test control group design, this research wasconducted in Pertina Lombok Tengah Regency. The research subjects involved 15 people divided into two groups, 7 and 8 samples respectively. The control group given only shadow shot while treatment group given common shot. This training held 3 times a week in six weeks for both groups. The analyses data with independent t-test. Researcher found The endurance of arm muscles (p<0,05) that training in twu group his’not significant to VO2max (p>0,05).Keywords: Shadow blows, regular blows, Boxing, VO2max and Endurance of arm Muscl


2017 ◽  
Vol 53 (3) ◽  
pp. 180
Author(s):  
Alis Nur Diana ◽  
Reny I’tishom ◽  
Sri Agus Sudjarwo

Lead that enters the body may lead to increased production of ROS (Reactive Oxygen Species) that may affect reproductive system. Black cumin (Nigella sativa) extract contains high antioxidant, tymoquinone, that may be used to suppress oxidative stress induced by lead in animal experiments. This study aimed to prove that black cumin (Nigella sativa) extract improves the thickness of seminiferous tubular epithelium in Balb/c mice exposed to lead (Pb) acetate. This study used post-test only control group design. The subjects in this study were 30 Balb/c male mice (Mus musculus), divided into 5 groups. K-group: control group without lead acetate and black cumin extract, K+ group: group with 50 mg/kgBW of lead acetate for 28 days, P1 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.3 mg/gBW of black cumin extract for 21 days, P2 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.6 mg/gBW of black cumin extract for 21 days, and P3 group: treatment group with 50 mg/gBW of lead acetate for 28 days + 1.2 mg/gBW black cumin extract for 21 days. The results showed that the mean ± standard deviation of the highest thickness of seminiferous tubular epithelium was 45.80 ± 2.73 in the group that was exposed to acetate + 0.6 mg/gBW of black cumin (P2), and the lowest was 32.75 ± 4.07 in the group that was exposed to lead acetate (K+). The results were then analyzed by Anova test. The results showed that there was significant differences in the thickness of seminiferous tubular epithelium between P1, P2, P3 and K+. In conclusion, black cumin extract administration was proved to improve the thickness of seminiferous tubular epithelium in lead (Pb) acetate-exposed Balb/c mice (Mus musculus).


2017 ◽  
Vol 29 (2) ◽  
Author(s):  
Siti Rusdiana Puspa Dewi ◽  
Deratih Putri Utami AF ◽  
Indah Octantia ◽  
Nurul Ifadah

Introduction: The most common dental and oral health problem in the world is dental caries. In Indonesia, as many as 89% of children under 12 years old suffered from dental and oral disease. Lack of dental knowledge and awareness can be overcome by conducting intensive dental and health education to the community. 3D Dentobox is a simple three-dimensional game designed to deliver dental and oral health messages, including dental hygiene, ways to overcome dental health problems and some dental games. 3D Dentobox is an educational media prevention of caries in children. Methods: The method used was Quasi experiment with the type of pretest and posttest control group design. The sample of this study was 30 children aged 9-12 years old. The research subjects filled out a questionnaire containing information on the level of knowledge, attitudes, and actions in caries prevention efforts followed by outreaching to the media and 3D props Dentobox and was given back the same questionnaire to be filled. Data were collected and evaluated using Guttman scale and was analyzed by paired T test. Results: The results showed that the average level of knowledge, changing attitudes, and actions before and after use 3D Dentobox increased significantly (p<0.05). Conclusion: 3D Dentobox can be used as a media of education to prevent dental caries in children.


2019 ◽  
Vol 3 (1) ◽  
pp. 13
Author(s):  
Celia Lazarus ◽  
Henry Yonatan Mandalas ◽  
Winny Suwindere

Pendahuluan: Derajat keasaman (pH) saliva merupakan faktor kunci utama keseimbangan demineralisasi dan remineralisasi gigi. Demineralisasi email terjadi pada keadaan pH <5,5 dan terjadi dalam waktu beberapa menit setelah asupan sukrosa. Saliva memiliki peran signifikan dalam proses meningkatkan pH rongga mulut sehubungan dengan kemampuan buffering, yaitu kandungan bikarbonat yang dapat menetralkan pH sehingga mencegah enamel gigi dari demineralisasi. Tujuan penelitian ini untuk mengukur atau menilai peranan keju Brie dalam menaikan pH saliva. Metode: Penelitian ini merupakan eksperimental semu bersifat komparatif. Jumlah subjek penelitian adalah 32 orang yang dibagi menjadi kelompok perlakuan, yaitu mengonsumsi keju Brie dan kelompok kontrol, yaitu tidak mengonsumsi keju Brie. Subjek penelitian diukur nilai pH saliva awal dan akhir menggunakan pH test strip. Hasil: Rerata selisih nilai pH awal dan akhir pada kelompok perlakuan adalah sebesar 0,48 dan pada kelompok kontrol adalah sebesar -0,29. Rerata selisih pH saliva pada kelompok perlakuan, yaitu mengonsumsi keju Brie, lebih besar dibandingkan dengan kelompok kontrol, dengan perbedaan yang signifikan (p<0,05). Simpulan: Mengonsumsi keju Brie efektif dalam meningkatkan pH saliva.Kata kunci: Efektivitas, keju Brie, pH saliva ABSTRACTIntroduction: Salivary acidity degree (pH) is the main key factor in the balance of tooth demineralisation and remineralisation. Enamel demineralisation occurs at the pH < 5.5 and occurs within minutes after sucrose intake. Saliva has a significant role in the process of pH increase in the oral cavity due to the buffering ability, which is the bicarbonate content which able to neutralise the pH value to prevent tooth enamel demineralisation. The purpose of this study was to measure or assess the effect of Brie cheese consumption in raising the salivary pH. Methods: This study is a quasi-experimental comparative. The number of research subjects was 32 people who were divided into treatment groups which consumed Brie cheese, and the control group which did not consume Brie cheese. Each research subject was measured the initial and final salivary pH values using a pH test strip. Results: The average difference in the initial and final pH values of the treatment group was 0.48, and in the control group was -0.29. The average difference of the salivary pH in the treatment group, which was consuming Brie cheese, was higher than the control group, with a significant difference (p < 0.05). Conclusion: Consuming Brie cheese is effective in increasing the salivary pH.Keywords: Effectiveness, Brie cheese, salivary pH


2021 ◽  
Vol 16 (1) ◽  
pp. 1
Author(s):  
Luluk Hidayatul Maghfiroh ◽  
A Fahmy Arif Tsani ◽  
Fillah Fithra Dieny ◽  
Etisa Adi Murbawani ◽  
Rachma Purwanti

Low consumption of vegetable and fruit in children was still commonly found. Appropriate and effective nutrition education methods are needed to increase knowledge and consumption of vegetable and fruit in children. This study aimed to know the effectiveness of nutrition education through socio-dramatic method of vegetable and fruit knowledge and consumption in children 5-6 years old.This study was a quasi-experiment with pre-test and post-test control group design. Research subjects were 36 Taman Putra Kindergarten children who were divided into treatment group who were given nutrition education with socio-dramatic method, and control group who were given nothing. The improvement of nutrition knowledge was assessed by pre-test and post-test with pictured form, while vegetable and fruit consumption were assessed by 3 Days Food Record instrument. A total of 15 subjects had low fruit vegetable knowledge, and more than 50% of subjects had less fi ber intake, types of vegetable consumption <7 types, the amount of vegetable intake <250 grams, and fruit <150 grams. The provision of nutritional education through sociodrama method was effective in increasing knowledge of fruit vegetables in pre-school children (p <0.001), as seen from the increase in average knowledge of treatment group compared to control group. But unfortunately, nutrition education through sociodrama method has not been able to signifi cantly increase fruit vegetable consumption in terms of both quantity and type (p> 0.05).


2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Suyatno Hadi Saputro ◽  
Nugroho Ari Wibowo

The purpose of this study was to determine the effectiveness of Chinese jatropha sap on the development of incision wound bacteria colonization in experimental animals Mus musculus Strain Balb / cThe design of this study used a true experiment post only control group design by administering Chinese jatropha tree sap to male mice (bal mus / musculus) strains aged 2 to 2.5 months and weighing 20-30 grams with a total of 30 mice. Mice made incision wounds and observed the number of colonies in the inflammatory and proliferation phases of Chinese jatropha sap and compared with bioplacentonsThe results of the study in the treatment group were 410 bacteria on average while the control group were 585 bacteria in the inflammatory phase. Furthermore, in the proliferation phase the treatment group averaged 39 bacteria while in the control group there were an average of 79 bacteria. Independent t-test results in the inflammatory phase showed that р = 0.042 <α = 0.05 Furthermore, in the proliferation phase р = 0.041 <α = 0.05, in both phases there was an effect of giving Chinese jatropha tree sap to the amount of bacterial colonization in animals try Mus musculus Strains Balb / c.Chinese jatropha tree sap contains alkaloids, saponins, flavonoids, tannins, and anti-microbial β-lactams so as to stop the development of bacterial colonies in wounds. It is hoped that the Chinese jatropha sap can be an alternative in the initial treatment of open wounds and incisions in the limitations of medicine when a disaster occurs.


2020 ◽  
Vol 9 (1) ◽  
pp. 87
Author(s):  
Nurul Fatimah Susanti ◽  
Reny I’tishom ◽  
Siti Khaerunnisa

Background : Flavanoids are antioxidants that can prevent the negative effects caused by lead. The flavonoids contained in the Solanum betaceum extract have the potential to prevent the adverse effects of lead on the reproductive tract of rats because it can prevent oxidative stress. The purpose of this study was to prove the effect of Solanum betaceum extract on spermatogenic cells of mice exposed to lead acetate. The hypothesis in this study is that there is an effect of the administration of Solanum betaceum extract to an increase in the number of spermatogenic cells of mice (Mus musculus) exposed to lead Method : this type of research was a true experimental laboratory (true experimental), the research design uses a randomized posttest only control group design approach. The total sample of 40 heads was divided into 5 groups. Group K-: control group without the provision of lead acetate and Solanum betaceum extract, group K +: group with 75 mg / KgBB of lead acetate for 32 days, group P1: group of treatment with 75 mg / KgBB of lead acetate extract + Solanum betaceum extract for 31 days + group 100 mg / gBB for 35 days, group P2: treatment group with 75 mg / kg lead acetate for 31 days + Solanum betaceum extract 200 mg / KgBB for 35 days, and group P3: treatment group with 75 mg lead acetate KgBB for 31 days + Solanum betaceum extract 400 mg / gBB for 35 days.. Results : The mean ± standard deviation of the highest cell spermatogenic was highest 2107.88±78.70.Conclusion : The administration of Solanum betaceum extract can increase the thickness of the seminiferous tubules epithelium and the diameter of the seminiferous tubules in rats (Mus musculus) which are exposed to lead acetat.


Author(s):  
Ismi Puji Astuti

Background: The first labor anxiety is an unpleasant psychological condition due to physiological changes that cause psychological instability. The condition of excessive anxiety, worry, fear without cause, and stress, causes the muscles of the body to tense up, especially the muscles in the birth path to become stiff and hard, making it difficult to expand. This study analyzed the effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth. Methods: Quasi experimental design with nonrandomized pretest-postest control group. The sampling technique used was purposive sampling with 34 research subjects. 17 people for the treatment group were given pregnancy exercise 8 times, and 17 people for the control group were given health education about preparation for childbirth. Results: The results of data analysis using the Wilcoxon signed rank test showed that the average reduction in the treatment group was 8.00 and the control group was 3.00. The effect of pregnancy exercise on third trimester primigravida anxiety in the treatment group with a p-value (0.000) <(0.05), while the control group with a p-value (0.180)> (0.05). Conclusion: There is an effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth in the Wuryantoro Health Center work area. 


2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Yurike Septianingrum ◽  
Nety Mawarda Hatmanti

Introduction: Menstrual pain or dysmenorrhea is a common problem that is often complained of by women during menstruation. Menstrual pain can interfere learning activities, especially final year students in completing their thesis. The purpose of this study were to analyze the effect of endorphin massage on menstrual pain in final year nursing students of UNUSA. Method: This study using an experimental with pre-test and post-test control group design. Research subjects of this study was recruited using simple random sampling who was included inclusion and exclusion criteria. Research subjects in this study was of 46 students who were then divided into two groups, 23 students into intervention group and 23 students into control group. Data were collected by using observation with VAS. Data were analyzed by using Wilcoxon and Mann-Whitney U test. Result and Analysis: The results showed that: 1) There was difference VAS score between pre test and post test in intervention group (p= 0,000), 2) There was difference VAS score between intervention and control group (p= 0,017). Conclusion: Endorphin massage could reduce menstrual pain in final year nursing student of UNUSA. Further research, is expected that endorphin massage can be compared with music therapy to reduce menstrual painKeywords: endorphin massage, menstrual pain, primary dysmenorrhea


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